Levan is a high-valued polysaccharide of fructose produced by several microbial species. These polysaccharides have been described as effective therapeutic agents in some human disease conditions, such as cancer, heart diseases and diabetes.
Trang 1Available online 27 August 2021
0144-8617/© 2021 Elsevier Ltd This article is made available under the Elsevier license (http://www.elsevier.com/open-access/userlicense/1.0/)
Production of levan from Bacillus subtilis var natto and apoptotic effect on
SH-SY5Y neuroblastoma cells
Amanda Mota Vieiraa, Farrah Zahedb, Alessandre Carmo Crispimc, Edson de Souza Bentoc,
Rafael de Freitas Oliveira Françad, Irapuan Oliveira Pinheiroa, Luis A Pardob, Bruno
Melo Carvalhoa,*
aBiological Sciences Institute, University of Pernambuco (ICB/UPE), Recife, Brazil
bMax Planck Institute for Experimental Medicine, G¨ottingen, Germany
cInstitute of Chemistry and Biotechnology, Federal University of Alagoas, Maceio, Brazil
dDepartment of Virology, Aggeu Magalh˜aes Institute (IAM/FIOCRUZ Pernambuco), Recife, Brazil
A R T I C L E I N F O
Keywords:
Exopolysaccharide
Neoplastic cells
Live-cell analysis
A B S T R A C T Levan is a high-valued polysaccharide of fructose produced by several microbial species These polysaccharides have been described as effective therapeutic agents in some human disease conditions, such as cancer, heart diseases and diabetes The objective of this study was to examine the effect of levan (β-(2 → 6)-fructan) produced
through sucrose fermentation by B subtilis var natto on the proliferation rate, cytotoxicity, and apoptosis of
human neuroblastoma SH-SY5Y cells It was obtained 41.44 g/L of levan in 18 h by biotechnological fermen-tation and SH-SY5Y cells were exposed to 1000 μg/mL of levan The treatment with 1000 μg/mL of levan induced apoptosis in SH-SY5Y cancer cells by the significant increase in Annexin V/7-AAD and caspase 3/7 activation, but did not decrease proliferation or triggered a cytotoxic effect 1000 μg/mL levan treatment is a promising therapeutic strategy for SH-SY5Y neuroblastoma cells
1 Introduction
Cancer is a disease characterized by the abnormal growth of cells,
which can invade adjoining parts of the body and/or spread to other
organs, affecting almost any part of the body According to the World
Health Organization, cancer is one of the leading causes of death
glob-ally, and it was responsible for 10 million deaths in 2020 (World Health
Organization, 2020) This disease rarely occurs before the age of 20
years, but childhood cancers do exist, raising a range of medical,
psy-chological, ethical, and societal concerns (Steliarova-foucher et al.,
2017)
Neuroblastoma (NB) is the most common neoplasm during infancy,
usually diagnosed in the first year of life Prevention and screening are
not possible, due to the formation of this tumor during sympathetic
nervous system development (Cheung & Dyer, 2013) The tumorigenesis
of neuroblastoma involves both embryonic and oncogenic factors, as this
is a highly heterogeneous and complex disease (Kågedal, 2009) Many
factors, such as age and stage of the disease at diagnosis, and molecular,
cellular, and genetic characteristics of the tumor, determine whether it
will spontaneously regress or metastasize and become highly malignant (Cheung & Dyer, 2013)
The standard treatment for NB is based on the combination of chemotherapeutic drugs such as doxorubicin, vincristine, cyclophos-phamide, and cisplatin; however, chemoresistance occurs and the tumor becomes highly aggressive and metastatic (Tibullo et al., 2018) In search of new therapeutic approaches, several compounds from pro-cesses have been developed and tested in the SH-SH5Y neuroblastoma cell line (Biedler, Roffler-tarlov, Schachner, & Freedman, 1978) Levan are fructose polysaccharides that are produced by plants and many microorganisms Consisting almost solely of fructosyl residues
linked via the β-2, 6 carbons, these fructan molecules are packed into
nano-sized, spherical forms, providing them with a remarkably low intrinsic viscosity (Arvidson, Rinehart, & Gadala-Maria, 2006)
Micro-organisms, such as Bacillus subtilis (Veerapandian, Ramiah, & Varadhan,
2020), Bacillus aryabhattai (Nasir et al., 2020), Brachybacterium
pheno-liresistens (Moussa, Al-qaysi, Thabit, & Kadhem, 2017), Gluconobacter
strains (H¨ovels, Kosciow, Kniewel, Jakob, & Deppenmeier, 2020) can produce levan from sucrose, syrups, or molasses in submerged cultures,
* Corresponding author at Av Gov Agamenon Magalh˜aes - Santo Amaro, Recife, PE 50100-010, Brazil
E-mail address: bruno.carvalho@upe.br (B.M Carvalho)
Contents lists available at ScienceDirect Carbohydrate Polymers
journal homepage: www.elsevier.com/locate/carbpol
https://doi.org/10.1016/j.carbpol.2021.118613
Received 15 June 2021; Received in revised form 20 August 2021; Accepted 23 August 2021
Trang 2and possible levan structural variability may be induced by different
production conditions and molecular weight (Hundschell, Jakob, &
Wagemans, 2020) Levansucrase EC 2.4.10 are fructosyltransferases
enzyme (E.C.2.4.1.9) that catalyzes β-(2,6)-levan synthesis through
su-crose hydrolysis to glucose and fructose, and also catalyzes formation of
fructooligosaccharides (FOS) It has been considered one of the most
important enzymes in levan's biotechnological field, with high levels of
efficiency (using 100 g/L of sucrose as substrate in production medium,
it was obtained 47 g/L of levan yield) (Ragab et al., 2019) The microbial
source of levansucrase determines the molecular weight, degree of
branching (Runyon, Nilsson, Ulmius, & Castro, 2014), diameter,
intrinsic viscosity, stability, and functionalities such as immunogenic
activity and adhesive strength Since the discovery of the molecular
versatility of levan, researchers have been attracted by the potential
health benefits of these natural product
Regarding anticancer activity, levan molecular weight execute an
important role (Calazans, Lima, de França, & Lopes, 2000), although
researchers have not yet reached a consensus on the ideal molecular
weight for the treatment The antineoplastic activity of levan has been
widely investigated for the potential in treating hepatocellular and
gastric carcinomas (Yoon, Yoo, Cha, & Lee, 2004) (Abdel-fattah, Gamal-
eldeen, Helmy, & Esawy, 2012a; Cabral de Melo, Borsato, Macedo, &
Celligoi, 2015; Dahech, Belghith, Belghith, & Mejdoub, 2012; Esawy
et al., 2013; Sarilmiser & Oner, 2014); however, information regarding
the effects of levan in other cancer cell lines is lacking Thus, the aim of
this study was to produce levan by microorganism B subtilis var natto
and evaluate the effects of levan on cellular proliferation, cytotoxicity,
and apoptosis processes in an aggressive neuroblastoma cell line (SH-
SY5Y)
2 Material and methods
2.1 Microorganisms and reagents for culture medium
B subtilis var natto was purchased from GEM Cultures (Ft Bragg, CA,
USA) B subtilis var natto was kept on Nutrient Agar (NA) medium,
containing: agar (15 g/L), beef extract (3 g/L), sodium chloride (5 g/L),
peptone (5 g/L) And also nutrient broth (NB) was composed of beef
extract (3 g/L), peptone (1.5 g/L), and NaCl (5 g/L) were purchased
from Becton Dickinson (BD) (Franklin Lakes, New Jersey, USA)
MgSO4⋅7H2O, NaH2.PO4.2 H2O, and NaH2⋅PO4⋅12H2O were obtained
from Sigma Chemicals (San Luis, Missouri, USA) All reagents used had a
high level of purity (≥98% of purity grade)
2.2 Inoculum preparation
B subtilis var natto was maintained at 4 ◦C on NA medium and
subcultured every 15 days For activation, bacteria were cultured on NA
at 37 ◦C, pH 7.4, overnight, then colonies were transferred into 5 mL of
nutrient broth (pH 7.4) and incubated at 37 ◦C for 24 h, with agitation at
150 rpm Cell growth was determined by turbidimetry at λ = 660 nm
using a spectrophotometer (Bel Photonics, S˜ao Paulo, Brazil) and the
inoculum was 1% (v/v) After incubation, cells were transferred to a 60
mL Erlenmeyer flask for 24 h, shaking at 150 rpm Following, 540 mL of
levan production medium, a modification of that previously described
by da Costa et al (da Costa, 2005), composed of sucrose (250 g/L), urea
(2 g/L), yeast extract (5 g/L), KH2PO4 (1 g/L), K2HPO4 (8 g/L),
MgSO4⋅7H2O (1 g/L), FeSO4.7H2O (0.1 g/L), CuSO4⋅5H2O (0.0088 g/L),
MnSO4⋅H2O (0.0076 g/L), and ZnSO4⋅7H2O (0.01 g/L), was added under
the same conditions
2.3 Levan production by batch fermentation
Fermentation was carried out in a fully instrumented and computer-
controlled 5-L SL-135/E stirred tank bioreactor (Solab, S˜ao Paulo,
Brazil), equipped with a pH probe (Type In PRO® 3255; Mettler Toledo,
Columbus, Ohio, EUA) and a dissolved oxygen probe (Type In PRO®; Mettler Toledo) Pre-cultured medium (600 mL) were inoculated into
2400 mL of levan production medium and cultured for 18 h Agitation was provided by a three-blade impeller operated at 950 rpm Aeration was provided by a sprinkler, and the aeration rate was maintained at 3 vvm pH was maintained at 7.0 during cultivation and was monitored according to previous pH optimization studies (Chidambaram et al.,
2019; Gojgic-Cvijovic et al., 2019; Ragab et al., 2019) To maintain the
pH, 2 N NaOH and 2 N HCl were automatically added to the culture broth The fermentation temperature was maintained using a re- circulating water bath at 37 ◦C During fermentation, samples were extracted hourly to evaluate bacterial growth by turbidimetry at λ =
660 nm Levan production were performed in duplicate
2.4 Sucrose concentration
Sucrose concentration was determined using the High Performance Liquid Chromatography Serie 1200 (Agilent Technologies, Waldbronn, Germany) using a 300 × 7.8 mm Aminex HPX-87H column, with 9 μm particle size (Bio-Rad, Hercules, CA, USA) The mobile phase used was 5
mM sulfuric acid at a flow rate of 0.6 mL/min and a temperature of
20 ◦C Sucrose was used for the standard curve (1 g/L–5 g/L), and integration of the peak area was performed using ChemStation Rev B.04.02.98 software (Agilent Technologies)
2.5 Levan molecular weight
Levan was isolated from the cell-free culture fluid by centrifugation
at 18,500 ×g at 4 ◦C, followed by 0.22 μm filtration, and precipitated using 70% v/v of cold ethanol The precipitate was redispersed in 1 L of distilled water and dried in a Spray dryer MSDi 1.0 (LabMaq, S˜ao Paulo, Brazil), at a flow rate of 0.8 L/h, with a 140 ◦C inlet temperature and a 113.3 ◦C outlet temperature At the end of the operation, the powder was weighed, and the levan concentration was estimated in g/L The average molecular weight (Mw), number average molecular weight (Mn), and molecular weight of the highest peak (Mp) polydispersity index (PDI = Mw/Mn) of levan was determined using a high- performance size-exclusion chromatography system (GPC), models BOM007, INJ 003, and CRL008 (Waters, Santa Clara, CA, USA) coupled with four serially connected Shodex columns (SB 806 M HQ, SB 804 HQ,
SB 803 HQ, and SB 802 HQ) The polysaccharide sample was dissolved
in ultrapure water and filtered through a 0.22 μm Millipore filter (Merck, Newark, NJ, USA) before injection The mobile phase was 0.1 M sodium nitrate at a flow rate of 0.4 mL/min at room temperature, and the Waters 2414 refractive index was used for detection Pullulan (Shodex, Tokyo, Japan) was used as a standard for the correlation curve (MP 5800, MP 12,200, MP 23,700, MP 48,000, MP 44,200, MP 100,000,
MP 186,000, and MP 1,660,000 g/mol) Finally, data acquisition and processing were performed using Waters Empower 2 software (Walters, Santa Clara, CA, USA)
2.6 1 H and 13 C NMR spectroscopy
Chemical structure of levan was confirmed by NMR All NMR spectra were measured on a AvanceTM 600 spectrometer (Bruker, Madison, WI), 600.09 MHz for 1H and 150.89 MHz for 13C nuclei at 25 ◦C, using a
5 mm PABBO probe Spectra were obtained at 298 K in D2O (10 mg/mL), and the residual H1 chemical shift signal of D2O was used as reference for chemical shift in the hydrogen spectrum Homo- and heteronuclear two-dimensional (2D) spectra H–H COSY (correlation spectroscopy), H–C HSQC (Heteronuclear Single-Quantum Coherence) and H–C HMBC (Heteronuclear Multiple Bond Correlation) applying standard Brucker's pulse sequences were used for full assignment of the signals TOPSPIN 3.2 (Bruker) was used for data acquisition and processing
Trang 32.7 Cell culture
SH-SY5Y neuroblastoma cancer cells were purchased from the
American Type Culture Collection (ATCC, Rockville, MD, USA) and
maintained in culture with Dulbecco's Modified Eagle Medium (1×) +
GlutaMAX (GIBCO-Invitrogen, Carlsbad, CA, USA) supplemented with
15% FCS and antibiotic/antimycotic (1% penicillin/streptomycin), in a
humidified atmosphere at 37 ◦C and 5% CO2 The medium was changed
as needed, and cells were sub-cultured upon reaching ~85% confluence
2.8 Cell proliferation assay
Cellular proliferation rate was measured using a live-cell imaging
system (IncuCyte ZOOM, Essen Bioscience, Birmingham, UK) and the
corresponding software application Cells (1.0 × 105) were seeded into
96-well plates, and levan treatment was initiated after 24 h, in triplicate
Levan concentration ranged from 200 μg/mL to 1000 μg/mL, and
im-ages were recorded hourly over 1–5 days Proliferation rate (%) was
determined as cell confluence over time every hour
2.9 Caspase 3/7 activity assay
Apoptotic processes dependent on caspase 3/7 activation were
evaluated by live-cell imaging (IncuCyte ZOOM®; Essen Bioscience)
using the CellPlayer96-Well Kinetic Caspase-3/7 reagent containing the
caspase substrate DEVD coupled to the DNA intercalating dye NucView
488 (Incucyte Caspase 3/7 Green reagent; Essen Bioscience) at a
con-centration of 2 μM, which was added along with cell culture medium to
the different levan test concentrations (200, 600, 800, and 1000 μg/mL)
in 96-well plates The wells were analyzed until they reached 80%–90%
cell confluence The number of apoptotic cells per well was determined
in triplicate, as the number of green positive signals (in the green
channel; 488 nm)/mm2 over time
2.10 Cytotoxicity assay
IncuCyte ZOOM® (Essen Bioscience) was used to measure levan
cytotoxicity, as well as CellPlayer 96-Well Kinetic CellTox™ (Promega,
Madison, USA), containing asymmetric cyanine dye as a fluorescent
marker that enters dead cells and fluoresces upon binding to DNA,
providing a signal that is directly proportional to cytotoxicity Medium
and dye at a concentration of 2 μM were added at the same time as levan
(200, 600, 800, and 1000 μg/mL) The total number of cytotoxic dead
cells was counted in the green channel (488 nm) over time and
calcu-lated as the number of green positive signal/mm2
2.11 Annexin V apoptosis assay
Apoptosis assays were assessed by FACS Aria III flow cytometry (BD
Biosciences, Heidelberg, Germany) according to the manufacturer's
protocol, using the Annexin V-FITC Apoptosis kit (Thermo-Fisher,
Darmstadt, Germany) SH-SY5Y cells (105 cells per well) were seeded in
24 well plates, in triplicate, and incubated with 1000 μg/mL of levan for
96 h at 37 ◦C and 5% CO2 (three independent experiments) Thereafter,
the cells were collected and centrifuged (380 ×g for 5 min) at room
temperature, washed three times with PBS, and then resuspended in
binding buffer The cells were then stained with Annexin V and
7-amino-actinomycin D (7 ADD) as dead cell marker, and incubated for 15 min at
37 ◦C in the dark The cells were analyzed by flow cytometry using FITC
and PerCP-Cy5.5 channels, and 3.0 × 104 events were acquired Late
apoptotic cells were determined by double marking of the
fluorochromes
2.12 Statistical analysis
All experiments were performed in triplicate Data are expressed as
mean ± SEM Cell fluorescence was represented by the green object
fluorescence (GOF) unit The Student's t-test and one-way analysis of
variance (ANOVA) were used to compare differences between the
groups Values of p < 0.05 were considered statistically significant
3 Result and discussion
3.1 Evaluation of the levan produced by B subtilis var natto
The growth curve of B subtilis var natto and sucrose concentration in
media changed during fermentation, as shown in Fig 1 The microor-ganisms remained in the lag phase for the first 4 h, grew linearly for 6 h, and declined from the tenth hour until the end of levan production (Fig 1) In medium with an initial 250 g/L of sucrose, the substrate was completely consumed after 14 h of fermentation The final levan con-centration after drying was estimated at 41.44 g/L Similar findings were previously reported using fermentation with the same microor-ganism and initial sucrose levels, reaching 61 g/L at 24 h (Wu, Chou, & Shih, 2013) In addition, using Bacillus licheniformis NS03, which is of
the same genus, approximately 53 g/L of levan was produced after 48 h
of fermentation (Gojgic-Cvijovic et al., 2019) Taking into account the total concentration of levan and the duration of fermentation time,
B subtilis var natto effectively produced levan in bioreactor
3.2 Levan molecular weight description
Levan generated in this study showed a bimodal distribution of two distinct Mw; the larger proportion had a lower Mw (8.8 kDa), and the smaller portion had a higher Mw (~2201 kDa), and both had a relatively low polydispersity index (around 1.3) (Supplementary data, Fig S1; Supplementary data, Table S1)
Levan's physicochemical characteristics and biological potential are determined by the microorganism and production conditions (Oner, ¨ Hern´andez, & Combie, 2016; Tanaka, Oi, & Yamamoto, 1980) A narrow polydispersity index is important for the suitability to various
applica-tions, and values <2.0 are considered low (Wolff et al., 2000) However,
it is challenging to precisely determine this characteristic, due to the lack of suitable techniques and standards (Raessler, Wissuwa, Breul, Unger, & Grimm, 2008) (Barclay, Ginic-markovic, Cooper, & Petrovsky,
2010), and most studied physical parameter (molecular weight) and correlate to some biological application
Another aspect to be considered is the enzyme that microorganisms synthesize levan Levansucrase enzyme generate levan as a mixture of low- and high-molecular-weight fractions, making possible a wide range
of molecular weights (Srikanth, Reddy, Siddartha, Ramaiah, & Uppu-luri, 2015) Furthermore, the molecular weight of the enzyme itself can influence in levan structure and consequently, in its properties
Fig 1 Kinetic and molecular characteristics of levan production by B subtilis
var natto Growth (-■-) and sucrose concentration (-●-) during fermentation
Trang 4Levansucrase with low molecular weight has an optimal enzymatic
ac-tivity at pH 8.2 and 45 ◦C (Salama et al., 2019); and these parameters
may not reflect in highest levan yield considering the microorganism
and production conditions (Ua-Arak, Jakob, & Vogel, 2017) Taking this
into account, the levan molecular weight itself will depend on the
microorganism used, the production medium, production conditions
and the specific aspects of the enzyme being produced All these
com-plex factors may not be in harmony, being extremely important to
characterize the molecular weight of levan in each batch (Ua-Arak,
Jakob, & Vogel, 2017) Levan produced by the genus Bacillus essentially
consist of a mix but with high molecular weight predominating (
Cala-zans, Lima, de França, & Lopes, 2000; Dahech, Belghith, Belghith, &
Mejdoub, 2012; Yoo, Yoon, Cha, & Lee, 2004) In contrast to literature,
the levan produced in the present study by B subtilis var natto it was
observed predominance of levan with low molecular weight
(Supple-mentary data, Table S1) Also, some authors have reported that high
molecular weights (>500 kDa) are more associated with antitumor
ac-tivity (Calazans, Lima, de França, & Lopes, 2000; Tanaka, Oi, &
Yama-moto, 1980) However, the relationship between levan molecular
weight and antineoplastic activity needs further investigation As well as
considering the complete fermentation process (microorganism,
enzyme, production medium and condition) in able to optimize
pro-duction on industrial scale
3.3 NMR levan characterization
NMR spectroscopy was used to confirm the chemical structural of
levan produced by B subtilis var natto In 13C spectra of levan
(Sup-plementary data, Fig S2), six main peaks are visible (104.2, 80.3, 76.5,
75.3, 63.4, 60.1 ppm) with positions similar reported by most of levan,
despite the producing microorganism, characterized for NMR (Table 1)
The 13C NMR, DEPT 90 and DEPT 135 were performed to report the
structure and their respective signals positions The absence of an
intense signal, at 104.2 ppm, present in 13C NMR spectrum, but not in
DEPT90 and 135 spectra was evidence for quaternary carbon of fructose
compounds (Supplementary data, Fig S2) disclosed by other authors
(Cai, Liu, Li, & Lu, 2019; Duymaz et al., 2019; Magri et al., 2020) The
spectrum of 1H–13C HSQC NMR (Fig 2) showed all expected
correla-tions for levan with the correlated signals (Table 2), and these
dis-placements are associated with corresponding to the resonance signals
from β-2,6 fructofuranose or levan (Aramsangtienchai, Kongmon,
Pechroj, & Srisook, 2020)
Additionally, the 1H NMR spectrum of levan (Supplementary data,
Fig S3) showed seven main proton signals in the ring proton region
3.5–4.1 ppm, implying that there are no anomeric protons in this fructan
(4.20 to 5.40 ppm), as described previously (Aramsangtienchai,
Kong-mon, Pechroj, & Srisook, 2020) Finally, COSY spectrum, allowed to
visualize the correlation between the vicinal and geminal hydrogens
(Supplementary data, Fig S4)
3.4 Effect of levan on proliferation in SH-SY5Ycells
Four different concentrations of levan (200, 600, 800, and 1000 μg/ mL) were used to generate a concentration-effect curve for the effect of levan on cell proliferation The proliferation rate (%) was not altered at any of the concentrations during 96 h of treatment (Fig 3) In other studies, levan showed an antiproliferative effect in other cancer cell lines, none of them related to neuroblastoma (Patel & Agrawal, 2019;
Poli et al., 2009; Sarilmiser & Oner, 2014; Tanaka, Oi, & Yamamoto,
Table 1
Chemical shifts in the 13C NMR spectra of levan produced by B subtilis var natto compared to other sources
Chemical shifts (ppm)
na – not available
Fig 2 1H–13C correlated NMR spectrum of B subtilis var natto levan
Table 2
1H, 13C, HSQC, HMBC and COSY NMR data (δ — ppm; J — Hz) for levan H/
13 C
13 C COSY δ 1 H
1 60.1 3.58 (H-1, d, J = 12.80 Hz);
3.67 (H-1, d, J = 12.80 Hz) 76.5, 104.3
2 104.3
4 75.3 4.00 (H-4, t, J = 18,0 Hz) 63.5, 76.5 4.09,
3.86
3.62
6 63.4 3.62 (H-6, dt, J = 72.5 Hz, J =
15.5 Hz);
s, singlet; d, doublet; t, triplet
Trang 51980; Yoon, Yoo, Cha, & Lee, 2004) The evaluation in most of the
literature is performed using the MTT of other colorimetric test, while in
the present study, proliferation was evaluated in real time using a live-
cell analysis system, which is an extremely sensitive method
3.5 Cytotoxic effect of levan in SH-SY5Ycells
A kinetic concentration-effect curve was developed for levan
treat-ment and showed that levan did not exert cytotoxicity at up to 96 h of
treatment (Fig 4) under any of the experimental conditions In several
studies, it has been reported that levan exhibit selective cytotoxic
ac-tivity, affecting some cell lines more than others, especially in human
hepatocellular carcinoma (HepG2) cells In those studies, the cytotoxic
effect was quantified through the MTT colorimetric assay (Abdel-fattah,
Gamal-eldeen, Helmy, & Esawy, 2012a; Sarilmiser & Oner, 2014)
Be-sides, levan are not cytotoxic when used at 1000 μg/mL against healthy
strains of human fibroblasts, osteoblasts, and murine macrophages
(Dom˙zał-Kędzia et al., 2019; Gonz´alez-Garcinu˜no et al., 2017)
3.6 Levan apoptotic effect in SH-SY5Ycells
In order to investigate the role of levan in neoplastic progression, we used the human neuroblastoma (NB) SH-SY5Y cell line Neuroblastoma
is aggressive, metastatic, and one of the most common cancers in infants (Goodman, Gurney, Smith, & Olshan, 1999; Gurney, Smith, & Ross,
1999; Khan, Pandian, Ramraj, Aravindan, & Herman, 2015) SH-SY5Y cells are regularly used in nervous system research for Parkinson's, Alzheimer's, Huntington disease, and other neurodegenerative disorders (Li, Peng, Deng, Li, & Tian, 2020; Rakshit et al., 2020; Schilling et al.,
2019; Yeo et al., 2018) The kinetics of activation of the apoptosis executor caspase 3/7 was evaluated at different concentrations for 96 h (Fig 5) Levan led to a significant increase in the number of cells posi-tively labeled for active caspase3/7 in SH-SY5Y cells after 72 h of treatment with 1000 μg/mL compared with the untreated control group
(Control 232.25 GOF ± 11.33 vs 1000 μg/mL Levan-treated 275.36 GOF ± 11.67) To confirm this result, we evaluated apoptotic processes using flow cytometry in triplicate, with negative and positive controls (Fig 6a) We observed that the number of cells treated with 1000 μg/mL levan at 96 h and labeled with 3/7 caspase was significantly increased
(Control 13.63 GOF ± 1.24 vs Treated 39.46 GOF ± 3.66) compared to
the control group The number of double positive apoptotic cells (Fig 6b) confirmed that levan induced apoptosis in SH-SY5Y cells, increasing the number of double-labeled cells in the late stage of apoptosis Taking into account molecular weight of levan (around 8.8 kDa), it can be speculated that apoptotic effect on neuroblastoma cells may be due to increased penetration in the cell by the size of the poly-saccharide We demonstrated that levan induced apoptosis by the annexin/7ADD double stain in NB cells and that these cells had a sig-nificant increase in caspase 3/7 activation for up to 72 h Other authors
found similar results using B subtilis NRC1aza and Halomonas smyrnensis
AAD6T, but levan's therapeutical application was tested in HepG2 and human breast cancer (MCF-7) cells, respectively Levan treatment of
100 μg/mL and 1000 μg/mL, separately, activates caspase 3 in different cancer lines (Abdel-fattah, Gamal-eldeen, Helmy, & Esawy, 2012a;
Queiroz et al., 2017; Sarilmiser & Oner, 2014) Considering that levan is
a carbohydrate and its specific effect on apoptosis, the mechanism used
to activate this pathway is not yet known It can be speculated that levan activates the apoptosis cascade in neuroblastoma cells through an intermediary, a membrane protein that functions as a levan transporter
or cellular signal transducer (Brodie & Blumberg, 2003; Carneiro & El- Deiry, 2020) Further experiments are needed to support this theory
Fig 3 Proliferation rate of SH-SY5Y cells after treatment with different
con-centrations of levan Control group (-●-), 200 μg/mL levan (-■-), 600 μg/mL
levan (-▴-), 800 μg/mL levan (-▾-), and 1000 μg/mL levan (-◆-) Results are
expressed as mean ± SEM
Fig 4 Levan cytotoxic effect on SH-SY5Y cells after treatment with different
concentrations of levan Control group (-●-), 200 μg/mL levan (-■-), 600 μg/
mL levan (-▴-), 800 μg/mL levan (-▾-), and 1000 μg/mL levan (-◆-) Results are
expressed as mean ± SEM
Fig 5 Kinetic activation of caspase 3/7 in SH-SY5Y cells treated with levan for
up to 96 h Control group (-●-), 200 μg/mL levan (-■-), 600 μg/mL levan (-▴-),
800 μg/mL levan (-▾-), and 1000 μg/mL levan (-◆-) Caspase 3/7 was analyzed
by the recognition motif (DEVD) to a DNA intercalating dye, and real-time quantification pictures were captured and analyzed Data are expressed as the mean of arbitrary fluorescence units ± SEM Replicates, n = 3
Trang 64 Conclusion
In the current study, levan was produced by B subtilis var natto, and
41.44 g/L of yield was obtained after purification, consisting of mostly
low molecular weight units Additionally, our results indicated that
treatment of a neuroblastoma cell line (SH-SY5Y) with 1000 μg/mL of
levan exhibited no effect on proliferation and lacked the cytotoxicity
However, levan showed apoptotic effect in neuroblastoma cells, by
caspase 3/7 activation which increases over time Nonetheless, further
investigations on purification parameters, application in other
neoplastic cell lines with description of the action mechanism of levan
are needed, together with in vivo testing
CRediT authorship contribution statement
Amanda Mota Vieira: Conceptualization, Methodology, Formal
analysis, Investigation, Data curation, Writing – original draft, Writing –
review & editing Farrah Zahed: Conceptualization, Methodology,
Formal analysis, Investigation Alessandre Carmo Crispim: Formal
analysis, Investigation, Writing – original draft Edson de Souza Bento:
Supervision, Formal analysis, Investigation, Writing – original draft
Rafael de Freitas Oliveira França: Supervision Irapuan Oliveira
Pinheiro: Supervision Luis A Pardo: Supervision, Writing – original
draft Bruno Melo Carvalho: Conceptualization, Formal analysis,
Writing – original draft, Writing – review & editing, Supervision, Project
administration
Acknowledgments
This study was financed in part by the Coordenaç˜ao de
Aperfeiçoa-mento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001
and PDSE scholarship - 88881.132835/2016-01 (AMV) The authors
also thank the Fundaç˜ao de Amparo `a Ciˆencia e Tecnologia do Estado de
Pernambuco (FACEPE) – grant APQ-0411-2.07/18 and Conselho
Nacional de Desenvolvimento Científico e Tecnol´ogico (CNPq) for
financial support of this work
Appendix A Supplementary data
Supplementary data to this article can be found online at https://doi
org/10.1016/j.carbpol.2021.118613
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