Production of cell culture based vaccine and DNA Recombinant vaccine against Hydro Pericardium Syndrome (HPS) virus of poultry and their comparison through ELISA.

Production of cell culture based vaccine and DNA Recombinant vaccine against Hydro Pericardium Syndrome (HPS) virus of poultry and their comparison through ELISA Research and Research and Development[.]

Research and Development on Hydro Pericardium Syndrome

virus vaccine

M Salah-ud-Din Shah, DVM, M.Sc

(Hons) Biological Chemistry Division, NIAB

disease of poultry caused by Avi Adeno

Karachi, Pakistan So the syndrome was

1988)

year, mortality rate is 50-70%

STATUS OF AVAILABLE

VACCINES

• At present vaccines available in

market are produced from crude

methods e.g formalized liver

homogenate, these vaccines have

following short comings

• They carry proteins of livers

• Pathogens other than adeno virus if birds are infected

• These vaccines have very short shelf life (only 2-3 months)

• Vaccine failure is very common

PREVIOUS WORK

• Vaccine is Produced from crude method with slight modifications

• Virus is extracted & purified by

centrifugation

• Contains no liver protein

• Monitoring of chicks for any other disease to avoid other pathogens

• Addition of antibiotics to increase shelf life for one year

• Addition of Vitamin E and Selenium

Marker 1 2 3 4 6

116 kDa

66.2 kDa

45 kDa

35 kDa

25 kDa

14.4 kDa

18.2 kDa

No of Daily Mortalities in infected flock

after vaccination

Weakly Antibody titer of broilers after vaccination in treated & control group

Brochure of HPS Vaccine

PLAN OF WORK

production of standard vaccine

comparison through ELISA

CELL CULTURE BASED

VACCINE

hepatocytes cultured in vitro by following

cell culture media (M - 199) to obtain

monolayer in cell culture flasks

cells and allow the virus to grow on cells

cytopathic effects, quantification by ELISA, then use this virus as antigen in vaccine

DNA RECOMBINANT

VACCINE

• Extraction of viral DNA from samples of

infected chicks (Kao et al 2000)

• Amplification of HPS virus surface

antigen gene by PCR (Jiang et al.,1999)

• Ligate HPS virus surface antigen gene in

cloning vector

• Transform ligated Product in E coli

• Screen and select the recombined clone

• Confirm the clone by running the sample

on 1% agarose gel

antigen in vaccine production

EVALUATION OF VACCINES

vaccinating the birds first on small scale then on large scale in field conditions

vaccinated birds

determination of antibody titers to

1998)

Thanks