The importance of PARP-1 in repair is revealed by the fact that PARP-1 knockout mice are hypersensitive to alkylating agents Keywords base excision repair; DNA polymerase b; DNA repair;
Trang 1strand breaks from deterioration during repair in human cell extracts
Jason L Parsons, Irina I Dianova, Sarah L Allinson* and Grigory L Dianov
MRC Radiation and Genome Stability Unit, Harwell, Oxfordshire, UK
Spontaneously derived DNA lesions, such as base
modifications and abasic (AP) sites, and products of
base oxidation and alkylation are removed by the base
excision repair (BER) pathway [1] In human cells,
BER is initiated by the removal of the damaged base
by a DNA glycosylase to generate an AP site that is a
substrate for an AP endonuclease (APE1) which
cleaves the phosphodiester backbone 5¢ to the lesion,
creating a 3¢-OH and 5¢-deoxyribose phosphate (dRP)
terminus [2,3] The majority of the incised AP site
pro-ceeds via so-called ‘short-patch’ repair [4] whereby
DNA polymerase b (Pol b) catalyses the removal of
the 5¢-dRP lesion through a b-elimination mechanism
and also inserts the correct nucleotide to fill the gap
[5,6] The remaining nick in the DNA backbone is
then sealed by X-ray cross-complementing gene 1
(XRCC1)–DNA ligase IIIa complex [7–9]
BER proteins also participate in processing of DNA single-strand breaks (SSB) and one-nucleotide gaps: Pol b is involved in gap filling, XRCC1–DNA ligase IIIa complex is involved in ligation, and APE1 partici-pates in processing of blocked 3¢-ends [10,11] Further-more, other proteins, such as polynucleotide kinase and poly(ADP-ribose) polymerase-1 (PARP-1) have been implicated in the repair of SSBs [11–13] PARP-1
is thought to be involved in BER and strand-break processing, as it has a high binding affinity for SSBs [14] On binding to the strand break, PARP-1 catalyti-cally synthesizes poly(ADP-ribose) polymers from NAD+ that covalently modify proteins, of which PARP-1 itself is a target Subsequently PARP-1 disso-ciates from the strand break [15] The importance of PARP-1 in repair is revealed by the fact that PARP-1 knockout mice are hypersensitive to alkylating agents
Keywords
base excision repair; DNA polymerase b;
DNA repair; poly(ADP-ribose) polymerase-1
(PARP-1); XRCC1
Correspondence
G L Dianov, Radiation and Genome
Stability Unit, Medical Research Council,
Harwell, Oxfordshire OX11 0RD, UK
Fax: +44 1235 841 200
Tel: +44 1235 841 134
E-mail: g.dianov@har.mrc.ac.uk
*Present address
Department of Biological Sciences,
Lancas-ter University, LancasLancas-ter LA1 4YQ, UK
(Received 13 December 2004, revised 19
January 2005, accepted 24 February 2005)
doi:10.1111/j.1742-4658.2005.04628.x
Base excision repair (BER), a major pathway for the removal of simple lesions in DNA, requires the co-ordinated action of several repair and ancillary proteins, the impairment of which can lead to genetic instability
We here address the role of poly(ADP-ribose) polymerase-1 (PARP-1) in BER Using an in vitro cross-linking assay, we reveal that PARP-1 is always involved in repair of a uracil-containing oligonucleotide and that it binds to the damaged DNA during the early stages of repair Inhibition of PARP-1 poly(ADP-ribosyl)ation by 3-aminobenzamide blocks dissociation
of PARP-1 from damaged DNA and prevents further repair We find that excessive poly(ADP-ribosyl)ation occurs when repair intermediates contain-ing scontain-ingle-strand breaks are in excess of the repair capacity of the cell extract, suggesting that repeated binding of PARP-1 to the nicked DNA occurs We also find increased sensitivity of repair intermediates to nuclease cleavage in deficient mouse fibroblasts and after depletion of
PARP-1 from HeLa whole cell extracts Our data support the model in which PARP-1 binding to DNA single-strand breaks or repair intermediates plays
a protective role when repair is limited
Abbreviations
APE1, apurinic ⁄ apyrimidinic endonuclease 1; BER, base excision repair; PARP-1, poly(ADP-ribose) polymerase 1; Pol b, DNA polymerase b; PVDF, poly(vinylidene difluoride); SSB, DNA single-strand break; WCE, whole cell extract; XRCC1, X-ray cross-complementing gene 1.
Trang 2and irradiation and that PARP-1 null cell lines display
symptoms of genomic instability after treatment with
DNA-damaging agents [16–18] Furthermore, the
involvement of PARP-1 in BER is also supported by
the ability of PARP-1 to interact with XRCC1 [7,19]
and Pol b [20] and the observation that PARP-1 is
required for the assembly of XRCC1 foci after
oxida-tive DNA damage [21,22] Although it has been
sug-gested that PARP-1 plays a role in BER, the reports
on PARP-1 involvement in BER are controversial
[14,23,24] and its function in BER is unclear
In this study, we addressed the role of PARP-1 in
BER and SSB repair and demonstrate that PARP-1
prevents excessive SSBs arising during BER or as a
result of direct DNA damage
Results
Cross-linking of BER proteins during repair
of damaged DNA
Although it has previously been shown that PARP-1
binds to nicked DNA and interferes with the repair
reac-tion, it is not clear whether this binding is an integral
part of BER and what the physiological significance is
A cross-linking protocol was used to examine the role of
PARP-1 during repair of damaged DNA [25] This
pro-tocol uses oligonucleotides containing a 3¢-biotinylated
end, which are used to form a uracil-containing duplex
oligonucleotide complete with a hairpin loop (Fig 1)
The oligonucleotide is subsequently bound to streptavidin
magnetic beads and incubated with HeLa whole cell
extract (WCE) before the addition of formaldehyde to cross-link proteins to DNA The beads are subsequently washed, the cross-links reversed, and released pro-teins separated by gel electrophoresis and identified by immunoblotting with the corresponding antibodies During incubation of the uracil-containing substrate with WCE, uracil-DNA glycosylase removes uracil from the substrate DNA, and APE1 incises the AP site, generating a strand break containing a 5¢-sugar phos-phate This is further processed by Pol b and XRCC1– DNA ligase IIIa heterodimer Cross-linking during incubation of the uracil-containing substrate with HeLa WCE revealed that PARP-1 was the first protein to be present at the substrate and it gradually dissociated from the substrate within 4 min of incubation, while Pol b and XRCC1–DNA ligase IIIa, which are required for strand break processing, are cross-linked more efficiently after PARP dissociation (Fig 2A; left panel) However, most PARP-1 cross-linking observed during the first 30 s was most probably due to its DNA-damage-independent binding to the 5¢ end, or the hairpin loop structure of the substrate oligonucleo-tide, as similar PARP-1 cross-linking was also observed
in the case of the control substrate (Fig 2A; right panel), although damage-specific PARP-1 binding was observed from 1 min onwards In contrast, cross-link-ing of Pol b and XRCC1 is highly damage-specific, as, using a control undamaged substrate, we were unable
to significantly cross-link any of these proteins (Fig 2A; right panel) The same filters were analyzed with antibodies raised against poly(ADP-ribose) poly-mers, and we found that bound proteins undergo sub-stantial poly(ADP-ribosyl)ation Interestingly, the peak
of poly(ADP-ribosyl)ation at 1 min of incubation using the uracil-containing oligonucleotide (Fig 2B, line 4) correlates well with the damage-specific binding of PARP-1 (Fig 2A, compare lines 4 and 10) Substan-tially less poly(ADP-ribosyl)ation was observed using the control oligonucleotide (Fig 2B; right panel) and occurred before 1 min of incubation of the substrate with WCE and was associated with damage-unspecific PARP-1 binding In comparison, poly(ADP-ribo-syl)ation using the uracil-containing oligonucleotide was still evident after up to 8 min of incubation with WCE These results suggest the involvement of PARP-1 and PARP-1 poly(ADP-ribosyl)ation during BER of a uracil-containing oligonucleotide
Inhibition of PARP-1 poly(ADP-ribosyl)ation prevents BER in cell extracts
To examine whether PARP-1 is involved in every sin-gle BER event on damaged DNA, or just simply
Fig 1 Structures of oligonucleotides used to construct 3¢-biotinylated
hairpin substrates Oligonucleotides were designed to contain the
complementary sequence with a TTTT hairpin loop and a
3¢-biotinylated moiety (designated with an asterisk) Substrates (1)
and (2) contain uracil and cytosine, respectively, which are
base-paired with guanine Substrates (3) and (4) contain a nick with
3¢-OH, 5¢-phosphate and 3¢-phosphate, 5¢-OH ends, respectively.
Trang 3randomly competes with BER enzymes for the
sub-strate, we incubated the uracil-containing
oligonucleo-tide with HeLa WCE in the presence of the
poly(ADP-ribosyl)ation inhibitor 3-aminobenzamide Preventing
PARP-1 poly(ADP-ribosyl)ation should block PARP-1
dissociation from nicked DNA and thus inhibit further
binding of BER proteins We found that 3-aminobenz-amide stimulated cross-linking of PARP-1 throughout the course of the reaction and completely blocked poly(ADP-ribosyl)ation (data not shown) It subse-quently blocked access of the substrate to BER pro-teins, as we were unable to cross-link XRCC1 or Pol b under these conditions (Fig 2C) These data suggest that PARP-1 binding to repair intermediates is always involved in BER and that poly(ADP-ribosyl)ation-dependent dissociation of PARP-1 is required for further BER progression
PARP-1 is not essential for binding of XRCC1 and
Polb to damaged DNA
As PARP-1 is the first protein to interact with a nicked AP site during BER, it was interesting to test whether XRCC1–DNA ligase IIIa and Pol b binding
to DNA is specifically associated⁄ affected by PARP-1 and poly(ADP-ribosyl)ation To test this, we generated HeLa WCE depleted of PARP-1 by using 3-amino-benzamide-Affigel beads Using immunoblotting analy-sis, we showed that, compared with the original HeLa extract (Fig 3A, WCE), the PARP-1-depleted extracts were 95% devoid of PARP-1 (Fig 3A, I.D.) without significantly affecting the concentrations of other BER proteins, such as Pol b and XRCC1 (data not shown)
On incubation of the uracil substrate with PARP-1-depleted extracts, Pol b and XRCC1 are still com-plexed to DNA (Fig 3B), although the concentrations peak at earlier time points than in the presence of PARP-1 and the amounts of protein subsequently plat-eau, as observed from 30 s up to 2 min As a result of faster binding, both Pol b and XRCC1 dissociate ear-lier from DNA There is also no significant formation
of poly(ADP-ribose) polymers in the absence of PARP-1 We thus conclude that the critical event in an
in vitro BER reaction is poly(ADP-ribosyl)ation and dissociation of PARP-1, rather than interaction of BER proteins with PARP-1
PARP-1 binding protects repair intermediates from deterioration
It was previously proposed that PARP-1 binding may protect DNA strand breaks from nuclease attack when BER enzymes are a limiting factor in repair [24] To test this hypothesis, we compared PARP-1 involvement
in the repair of a substrate containing either a 3¢-OH and 5¢-phosphate strand break or a 3¢-phosphate and 5¢-OH strand break The latter substrate requires poly-nucleotide kinase to dephosphorylate the 3¢ end and
to phosphorylate the 5¢ end before ligation and is
A
B
C
Fig 2 Involvement of PARP-1 in BER in human cell extracts (A) A
uracil-containing (left panel), or the corresponding control (right
panel) biotinylated hairpin substrate was bound to magnetic
strept-avidin beads before incubation with 100 lg HeLa WCE After
incu-bation, proteins were cross-linked to DNA using 0.5% (v ⁄ v)
formaldehyde, and the beads subsequently washed Cross-links
were reversed, proteins separated by SDS ⁄ PAGE (10% gel),
trans-ferred to PVDF membranes and analysed by immunoblotting with
the indicated antibodies (B) The membrane in (A) was stripped and
reprobed with antibodies raised against poly(ADP-ribose) polymers
(PAR) (C) A uracil-containing biotinylated hairpin substrate was
bound to magnetic streptavidin beads before incubation with
100 lg HeLa WCE in the presence of the poly(ADP-ribosyl)ation
inhibitor 3-aminobenzamide (1 m M ) After the times indicated,
pro-teins were cross-linked to DNA and the beads subsequently
washed Cross-links were reversed and proteins separated by
SDS ⁄ PAGE (10% gel), transferred to PVDF membranes and
ana-lysed by immunoblotting with the indicated antibodies Time zero
equates to cross-linking immediately after extract addition.
Trang 4expected to be repaired much more slowly than a
frank DNA nick Subsequently using 5¢-end-labelled
substrates, we showed that repair of the 3¢-OH and
5¢-phosphate strand break is accomplished within 4 min,
whereas only a small fraction of the 3¢-phosphate and
5¢-OH strand break was processed after 8 min
(Fig 4A,B) Correspondingly, repair of the latter
sub-strate involves more PARP-1 binding and more
poly-(ADP-ribosyl)ation, as observed in the formaldehyde
cross-linking assay (compare Fig 4C,D) Therefore, we
propose that persisting SSBs can cause several cycles
of PARP-1 binding and dissociation We further
spe-culate that this repetitive binding of PARP-1 is
required only when the number of unrepaired strand
breaks exceeds the amount of rate-limiting repair
enzyme, and it may play a protective role masking the
strand break from nuclease attack To further
demon-strate the protective role of PARP-1, we blocked repair
of a uracil-containing oligonucleotide substrate by removing deoxyribonucleotide triphosphates from the reaction mixture Under these conditions, repair is blocked at the stage of a one-nucleotide gap created
by the sequential action of uracil-DNA glycosylase, APE1 and Pol b, and PARP-1 is continuously poly (ADP-ribosyl)ated (Figs 5A and 6A) As the repair gap is not filled, the 18-mer repair intermediate is accu-mulated (Figs 5B and 6B) This repair intermediate can be attacked by cellular nucleases if not protected
by PARP-1 Indeed, we observed an increased rate of degradation of the 18-mer fragment in both PARP-depleted cell extract (Fig 5B,C) and cell extract prepared from PARP-knockout cells (Fig 6B,C) com-pared with the original HeLa cell extract or cell extract prepared from wild-type mouse cells, respectively Furthermore, addition of purified PARP-1 protein to PARP-1-deficient cell extracts restored the stability of the 18-mer repair intermediate
Discussion
We have used an in vitro cross-linking assay to study the role of PARP-1 protein during BER We find that PARP-1 binds to the incised AP site at the very early stages of BER, as has been previously observed by using photoaffinity cross-linking during repair in cell extract [26] We also find that inhibition of poly(ADP-ribosyl)ation by 3-aminobenzamide blocks PARP-1 dissociation and completely prevents further repair These data suggest that PARP-1 is always an integral part of the BER process and that processing of uracil
in human cell extracts may be divided into two major steps: removal of the damaged base and incision of the generated AP site as a first step and processing of the incised AP site as a second step These steps are clearly marked by PARP-1 binding (Fig 7) This is in agree-ment with previously published data [23,24] that suggest that BER is NAD+-dependent and that poly(ADP-ribosyl)ation is required for PARP-1 disso-ciation and subsequent BER progression It should be noted that PARP-1 antibodies are very specific and would not recognize poly(ADP-ribosyl)ated PARP-1 Therefore, the decreased binding of PARP-1 observed
in Fig 2A may be interpreted as either PARP-1 disso-ciation or poly(ADP-ribosyl)ation However, cross-linking dynamics in the same reaction support the model in which PARP is being poly(ADP-ribosyl)ated first and then dissociates from DNA The level of damage-specific PARP-1 cross-linking was maximal at 0.5 min (binding) and then decreased after 2 min of the repair reaction [poly(ADP-ribosyl)ation and disso-ciation from DNA] In contrast, the presence of other
A
B
Fig 3 PARP-1 is not required for assembly of BER proteins on
damaged DNA in human cell extracts (A) HeLa WCE was depleted
of PARP-1 with 3-aminobenzamide-Affigel and confirmed by
analy-sing 30 lg of the corresponding extracts by SDS ⁄ PAGE (10% gel)
and immunoblotting with PARP-1 antibodies (B) A uracil-containing
biotinylated hairpin substrate was bound to streptavidin magnetic
beads before incubation with 100 lg HeLa WCE depleted of PARP-1.
After the times indicated, proteins were cross-linked to DNA and
the beads subsequently washed Cross-links were reversed and
proteins separated by SDS⁄ PAGE (10% gel), transferred to PVDF
membranes and analysed by immunoblotting with the indicated
antibodies The membrane was subsequently stripped and
reprobed with antibodies raised against poly(ADP-ribose) polymers
(PAR).
Trang 5BER proteins was maximal at 4 min (Fig 2A, lane 6).
This suggests that when there are sufficient amounts of
repair enzymes present, they efficiently remove PARP-1
from nicked DNA In agreement with the previous
finding that PARP-1 deficiency is not decreasing the
rate of short-patch BER [20,23,27,28], we also did not
find any effect of PARP-1 depletion on the
cross-link-ing efficiency of BER proteins durcross-link-ing repair of a
ura-cil-containing substrate Instead, removal of PARP-1
accelerated the loading and subsequent dissociation of
XRCC1–DNA ligase IIIa and Pol b on the nicked
DNA (compare Figs 2A and 3B) This finding is in a
good agreement with previous observations of
acceler-ated BER in PARP-1-deficient cell extracts [23]
However, the dispensability of PARP-1 from an
in vitroBER assay may not correctly reflect the role of
PARP-1 in cellular BER Numerous studies have
indi-cated that PARP-1 may play an important role in
living cells, as PARP-1-deficient cells are genetically
unstable and sensitive to DNA-damaging agents [16–
18] A mechanism by which PARP-1 actively recruits
repair proteins to the site of the strand break may be applicable to an in vivo situation in which the task of strand-break identification and accumulation of repair enzymes needed for repair is extremely difficult consid-ering the size of the human genome Indeed, poly-(ADP-ribosyl)ation-dependent accumulation of XRCC1
in repair foci after oxidative DNA damage and during SSB repair has recently been reported [21,22] There-fore, most probably the purpose of poly(ADP-ribo-syl)ation of PARP-1 is not only to allow PARP-1 to dissociate from the repair site but also to attract BER proteins In support of this idea, it has been shown that DNA ligase III and XRCC1 proteins have poly-(ADP-ribose)-binding motifs and that they and Pol b have also been shown to preferentially interact with poly(ADP-ribosyl)ated PARP [19,29,30]
We also find that, when repair is inefficient, unmodified PARP-1 can rebind to the substrate con-taining the strand break and probably accomplish sev-eral cycles if enough NAD+ is provided We further speculate that this rebinding plays a role in protecting
Fig 4 Repair of 5¢ or 3¢-phosphorylated nick-containing oligonucleotides and analysis of PARP-1 binding and poly(ADP-ribosyl)ation by cross-linking A 5¢-phosphorylated (A) or a 3¢-phosphorylated (B) nick-containing hairpin substrate was 5¢-end-labelled with [ 32 P]ATP[cP], bound to magnetic streptavidin beads, and incubated with 100 lg HeLa cell extract for the times indicated DNA–beads were subsequently purified and washed, and formamide loading dye added DNA was separated by SDS ⁄ PAGE (10% gel) The phosphorimage of the corresponding gels is shown The 5¢-phosphorylated (C) or the 3¢-phosphorylated (D) nick-containing hairpin substrates were also bound to streptavidin mag-netic beads before incubation with HeLa WCE for the times indicated and subsequent cross-linking with 0.5% formaldehyde The beads were subsequently washed, the cross-links reversed, and the proteins separated by SDS ⁄ PAGE (10% gel), transferred to PVDF membranes, and analysed by immunoblotting with the indicated antibodies Time zero equates to cross-linking immediately after extract addition.
Trang 6strand-break-containing DNA from nuclease attack.
Indeed we find that when repair is blocked, the
degra-dation of repair intermediates is more intensive in
PARP-1-deficient cell extracts, although we find that
human cell extracts are more responsive to PARP-1
deficiency than mouse cells In conclusion, our study
suggests that PARP-1 is always involved in BER of
DNA base lesions, and SSB and is important for
pre-venting degradation of excessive unrepaired DNA
strand breaks by cellular nucleases However, the
end-protecting function may be just one of the multiple
functions of PARP-1 in DNA metabolism
Experimental procedures
Materials
Synthetic oligodeoxyribonucleotides were purchased from
MWG-Biotech (Ebersberg, Germany) and gel purified on
a 20% polyacrylamide gel Streptavidin magnetic beads
and magnetic separation rack were purchased from New
England Biolabs (Beverly, MA, USA) Recombinant human PARP-1 was obtained from Alexis Biochemicals (Notting-ham, UK)
Antibodies XRCC1 (ab144) and DNA ligase III (ab587) antibodies were purchased from Abcam Ltd (Cambridge, UK) PARP-1 antibodies (C2-10) were purchased from Alexis Corpora-tion Ltd, and antibodies raised against poly(ADP-ribose) polymers were purchased from Trevigen (Gaithersburg,
MD, USA) Antibodies against rat Pol b were raised in rabbit and affinity purified as described [31]
Cell extracts Mouse embryonic fibroblasts derived from normal and PARP-1 knockout mice were kindly provided by G de Murcia (ESBS-CNRS, Strasbourg, France) Cells were maintained in Dulbecco’s modified Eagle’s medium supple-mented with 10% (v⁄ v) fetal bovine serum and antibiotics
A
B
C
Fig 5 Increased degradation of repair
inter-mediates in PARP-depleted cell extracts (A)
HeLa WCE was incubated with a
FAM-label-led oligonucleotide duplex in the absence of
deoxyribonucleotide triphosphates for the
times indicated, and aliquots of the reaction
mixture were separated by SDS ⁄ PAGE
(10% gel), transferred to PVDF membranes,
and analysed by immunoblotting with
poly(ADP-ribose) polymer (PAR) antibodies.
(B) HeLa and PARP-1-depleted HeLa WCE
was incubated with a FAM-labelled
oligonucleotide duplex in the absence of
deoxyribonucleotide triphosphates for the
times indicated before the addition of
formamide loading dye PARP-1-depleted
HeLa WCE was also complemented with
100 ng PARP-1 protein and incubated for
6 min before separation of the DNA by
SDS⁄ PAGE (20% gel) (C) The oligonucleotide
fragments were analysed using Quantity
One software, which indicates the relative
density of the fragments produced.
Trang 7HeLa cell pellets were purchased from Paragon (Aspen,
CO, USA) WCEs were prepared by the method of Manley
et al [32] and dialysed overnight against buffer containing
25 mm Hepes⁄ KOH, pH 7.9, 100 mm KCl, 12 mm MgCl2,
0.1 mm EDTA, 17% glycerol and 2 mm dithiothreitol
Extracts were divided into aliquots and stored at)80 C
Cross-linking assay
Streptavidin magnetic beads were blocked in 5% (v⁄ v)
non-fat milk and subsequently washed with Binding buffer
(20 mm Tris⁄ HCl, pH 7.5, 0.5 m NaCl, 1 mm EDTA) using
the magnetic separator rack Beads were then incubated
with hairpin substrates containing a 3¢-biotinylated moiety,
at room temperature with agitation for 30 min in Binding
buffer The DNA–beads were subsequently washed with
Wash buffer [25 mm Hepes, pH 7.9, 100 mm KCl, 12 mm
MgCl2, 1 mm EDTA, 5% (v⁄ v) glycerol and 2 mm
dithio-threitol] The DNA–beads (250 fmol DNA per reaction)
were then included in a reaction containing 100 lg HeLa
cell extract in 50 lL buffer containing 50 mm Hepes⁄ KOH,
pH 7.8, 50 mm KCl, 10 mm MgCl2, 0.5 mm EDTA,
1.5 mm dithiothreitol, 2.5% (v⁄ v) glycerol, 20 lm dCTP,
20 lm dATP, 20 lm dGTP, 20 lm dTTP, 2 mm ATP,
25 mm phosphocreatine (diTris salt; Sigma), 2.5 lg creatine phosphokinase (type I; Sigma), 0.25 mm NAD+and 1 lg carrier DNA (single-stranded 30-mer oligonucleotide) Reactions were incubated for the time indicated at 30C before cross-linking with formaldehyde (0.5%, final concen-tration) for a further 10 min at 30C (time zero equates to cross-linking immediately after extract addition) The beads were washed twice with 50 lL Wash buffer and
resuspend-ed in 20 lL SDS⁄ PAGE sample buffer [25 mm Tris ⁄ HCl,
pH 6.8, 2.5% (v⁄ v) 2-mercaptoethanol, 1% (w ⁄ v) SDS, 5% (v⁄ v) glycerol, 1 mm EDTA, 0.15 mgÆmL)1 bromophenol blue] Cross-links were reversed by heating for at least 2 h
at 65C, and proteins were separated on a SDS ⁄ 10% poly-acrylamide gel followed by transfer to a poly(vinylidene difluoride) (PVDF) membrane and immunoblot analysis with the indicated affinity-purified antibodies For direct comparison, proteins cross-linked from different substrates were analysed on the same immunoblot Extracts were also preincubated with 3-aminobenzamide (1 mm) for 20 min before the addition of DNA–beads and subsequent cross-linking for studies investigating PARP-1 poly(ADP-ribo-syl)ation inhibition Formaldehyde cross-linking is essential
A
B
C
Fig 6 Increased degradation of repair inter-mediates in PARP-deficient cell extracts (A) PARP-1+⁄ +WCE was incubated with a FAM-labelled oligonucleotide duplex in the absence of deoxyribonucleotide triphosphates for the times indicated Aliquots of the reac-tion mixture were separated by SDS ⁄ PAGE (10% gel), transferred to PVDF membranes, and analysed by immunoblotting with PAR antibodies (B) PARP-1 + ⁄ + and PARP-1 – ⁄ –
WCE were incubated with a FAM-labelled oligonucleotide duplex in the absence of deoxyribonucleotide triphosphates for the times indicated before the addition of forma-mide loading dye PARP-1–⁄ –WCE was also complemented with 100 ng PARP-1 protein and incubated for 6 min before separation
of the DNA by SDS ⁄ PAGE (20% gel) (C) The oligonucleotide fragments were analysed using Quantity One software, which indicates the relative density of the fragments produced.
Trang 8for detecting protein interactions with DNA-bound
strept-avidin magnetic beads, as no proteins were pulled down
without formaldehyde treatment
Depletion of PARP-1 from HeLa WCE with
3-aminobenzamide-Affigel
3-Aminobenzamide was covalently attached to Affigel
(Bio-Rad) by the method of Ushiro et al [33] Then
0.5 mL 3-aminobenzamide-Affigel (50% slurry) was
washed three times with 5 mL buffer containing 50 mm
Hepes⁄ KOH, pH 7.8, 50 mm KCl, 10 mm MgCl2, 0.5 mm
EDTA, 1 mm dithiothreitol, 17% (v⁄ v) glycerol,
100 lgÆmL)1 BSA and protease inhibitors (1 lgÆmL)1 each
of chymostatin, pepstatin and leupeptin and 1 mm
phenyl-methanesulfonyl fluoride) Then 1 mL WCE (17 mgÆmL)1)
was mixed with 0.5 mL 3-aminobenzamide-Affigel and
incubated overnight at 4C Affigel beads were removed
by centrifugation, and the extract was divided into aliquots and stored at)80 C
Repair assays on streptavidin beads Oligonucleotides were 5¢-end-labelled with [32
P]ATP[cP] using T4-polynucleotide kinase, and unincorporated label was removed on a Sephadex G-25 spin column To prepare the hairpin substrates, the oligonucleotides were incubated
at 90C for 3–5 min before slow cooling to room tempera-ture Substrates were then bound to streptavidin beads as described above before determination of repair capacity of HeLa WCE Streptavidin beads bound with radiolabelled hairpin substrates (250 fmol DNA per reaction) were incu-bated with 100 lg HeLa WCE in 50 lL Reaction buffer at
37C for the time indicated before addition of 12.5 lL
500 mm EDTA to stop the reaction Beads were washed twice with 50 lL 10 mm Tris⁄ HCl (pH 8.0), 100 mm EDTA and resuspended in 20 lL formamide loading dye DNA was separated on a 10% denaturing polyacrylamide gel, and the gel exposed to intensifying screens at 4C before analysis by phosphorimaging
Oligonucleotide degradation in WCE Reactions were reconstituted in a reaction mixture (10 lL) that contained 45 mm Hepes, pH 7.8, 70 mm KCl, 7.5 mm MgCl2, 0.5 mm EDTA, 1 mm dithiothreitol, 2 mm ATP,
2 mgÆmL)1BSA and a FAM (6-carboxyfluorescein)-labelled 30-mer oligonucleotide duplex containing a uracil residue at position 19 ( 5 ng, 500 fmol), in the absence of dNTPs to prevent repair of incised AP sites The reactions were initi-ated by addition of 20 lg cell extract and incubiniti-ated for the indicated time at 37C When 1-depleted or PARP-deficient WCEs were complemented with purified recombin-ant human PARP-1 protein, 100 ng (890 fmol) PARP-1 protein was added before incubation The reactions were stopped by the addition of 10 lL gel loading buffer After incubation at 90C for 3 min, the reaction products were separated by electrophoresis on a 20% denaturing poly-acrylamide gel
Acknowledgements
We are grateful to Gilbert de Murcia for providing PARP-1-knockout cells
References
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Fig 7 Involvement of PARP-1 in BER After processing of the
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poly(ADP-ribosyl)ation of PARP-1, automodified PARP-1 then
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intermediate to Pol b and XRCC1–DNA ligase IIIa, which perform
the remainder of the repair process If not enough repair enzymes
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