The area of the lesions depends on Keywords cell-to-cell spread; endocytosis; entry; filopodia; glycoproteins; herpes simplex virus; herpes viruses; phagocytosis; viral surfing Correspon
Trang 1Viral entry mechanisms: cellular and viral mediators of
herpes simplex virus entry
Jihan Akhtar1and Deepak Shukla1,2
1 Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois at Chicago, IL, USA
2 Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, IL, USA
Introduction
The herpesvirus family consists of over 100
double-stranded DNA viruses divided into a, b and
c subgroups [1] Only eight herpesviruses are known to
commonly infect humans and the remainder are
animal herpesviruses infecting a wide variety of animal
species All members of the herpesvirus family cause
lifelong latent infections and, structurally, all have a
linear, double-stranded DNA genome packaged into
an icosahedral capsid (Fig 1) [1] This capsid, in turn,
is enclosed by the tegument, a layer of proteins The
tegument is then covered by a bilayer lipid membrane
with embedded proteins and glycoproteins The
pro-tected DNA genome is essential for viral infectivity
Closely-related herpes simplex type-1 (HSV-1) and
type-2 (HSV-2) viruses are members of the
alphaher-pesvirus subfamily and are responsible for highly pre-valent infections among humans [2], although a number of common experimental animals also demon-strate susceptibility to HSV infections Symptomatic disease caused by HSV-1 is typically limited to cold sores of the mouth and keratitis in the eyes HSV-2, in contrast, is mostly responsible for genital lesions However, both viruses are capable of causing lesions
on identical body sites and both can cause life-threat-ening diseases in immunocompromised individuals, including newborns, patients with HIV or patients undergoing immunosuppressive treatment [1,2] Trans-mission among humans requires physical contact and often occurs during kissing (HSV-1) or sexual inter-course (HSV-2) The area of the lesions depends on
Keywords
cell-to-cell spread; endocytosis; entry;
filopodia; glycoproteins; herpes simplex virus;
herpes viruses; phagocytosis; viral surfing
Correspondence
D Shukla, 1855 W Taylor Street
(M ⁄ C 648), Chicago, IL 60612, USA
Fax: +1 312 996 7773
Tel: +1 312 355 0908
E-mail: dshukla@uic.edu
(Received 18 June 2009, revised 9
September 2009, accepted 18 September
2009)
doi:10.1111/j.1742-4658.2009.07402.x
Herpes simplex virus type-1 and type-2 are highly prevalent human patho-gens causing life-long infections The process of infection begins when the virions bind heparan sulfate moieties present on host cell surfaces This ini-tial attachment then triggers a cascade of molecular interactions involving multiple viral and host cell proteins and receptors, leading to penetration
of the viral nucleocapsid and tegument proteins into the cytoplasm The nucleocapsid is then transported to the nuclear membrane and the viral DNA is released for replication in the nucleus Recent studies have revealed that herpes simplex virus entry or penetration into cells may be a highly complex process and the mechanism of entry may demonstrate unique cell-type specificities Although specificities clearly exist, past and ongoing studies demonstrate that herpes simplex virus may share certain common receptors and pathways that are also used by many other human viruses This minireview helps to shed light on recent revelations on the herpes simplex virus entry process
Abbreviations
HS, heparan sulfate; HSPG, heparan sulfate proteoglycan; HSV-1, herpes simplex virus type-1; HSV-2, herpes simplex virus type-2; HVEM, herpesvirus entry mediator; 3-OST, 3-O sulfotransferase; PILR, paired immunoglobulin-like receptor.
Trang 2the inoculation site; therefore, sores are most
com-monly found on the mouth or genital areas Medical
professionals and others not wearing surgical gloves
could also acquire lesions on their fingers from the
virions shed from their patients’ vaginal mucosa
and⁄ or mouth This is commonly known as finger
herpes or herpetic whitlow
After initial infection, the virus remains latent in
neurons, a key feature of alphaherpesviruses [1]
Dur-ing this period, hosts are still capable of spreadDur-ing
infection to other humans via asymptomatic shedding
of the virions Reactivation of the virus can be a result
of a variety of environmental triggers, including
emo-tional or physical stress, which subsequently leads to
virus replication in epithelial cells and a lifetime of
intermittent mucocutaneous lesions [2] The ability
of the virus to avoid immune detection and establish
latency in a significant patient population (up to 80%
human adults for HSV-1 and approximately 40% for
HSV-2) is facilitated by its unique ability to
produc-tively enter cells of the epithelia for viral gene
expres-sion, replication and eventual spread from cell-to-cell
to innervating nerves and, ultimately, to trigeminal
(HSV-1) or sacral (HSV-2) ganglia for the
estab-lishment of latency Thus, HSV entry into host cells
marks the first and possibly most critical step in viral
pathogenesis
Five viral glycoproteins have been implicated in the
viral entry process: gB, gC, gD, gH and gL [3,4] All
but gC are essential for entry The initial interaction,
or binding to cells, is mediated via interactions of gC and⁄ or gB with heparan sulfate proteoglycans (HSPGs) F-actin-rich membrane protrusions called filopodia may facilitate attachment by providing HSPG-rich sites for the initial binding (Fig 1) Although gC is not essential for viral entry, its absence decreases the overall viral binding to cell surfaces [1] After the initial attachment to cells, the process of pen-etration begins The latter, depending on the host cell type and the mode of entry [4,5], may require fusion
of the virion envelope with the plasma membrane or with the membrane of an intracellular vesicle (Fig 1) [5] In either case, the membrane fusion requires essen-tial participation from viral glycoproteins gB, gD,
gH and gL Although gD is not considered to be a fusogen, other essential glycoproteins, more impor-tantly, gB and gH, demonstrate many characteristics
of viral fusion proteins [4,6] Similar to attachment, membrane fusion also requires participation from cellular receptors A number of unrelated receptors for
gD have been discovered These include nectin-1 and -2, herpesvirus entry mediator (HVEM) and 3-O sul-fated heparan sulfate (3-O HS) [3,7] The current, widely accepted model for membrane fusion suggests that binding of gD to one of its cognate receptors induces conformational changes in gD that mobilize a fusion active multi-glycoprotein complex involving gB,
gD, gH and gL (Fig 2) [8] Fusion of viral envelope
HSV surfing
Glycoproteins Tegument DNA
Envelope
HSPG
gD receptor
Cytoplasm
Nucleus
Fig 1 HSV virion and its two major modes of entry into cells Structural components of a typical HSV virion are shown (box) HSV virions can enter into cells via a pH-independent fusion of viral envelope with the plasma membrane (I) or, alternatively, via an endocytic pathway that may be phagocytosis-like (II) in terms of the viral uptake In both pathways, HSV particles may initially associate with filopodia-like mem-brane protrusions via HSPG Unidirectional transport of extracellular particles bound to filopodia (HSV surfing) then brings the particles closer
to the cell body for entry via interactions with the cellular receptors, including gD receptor and possibly gB receptor Fusion at the plasma membrane results in the release of the naked viral nucleocapsid in the cytoplasm for transport to the nucleus Similarly, endocytosis also requires fusion of the enveloped particles with the vesicular membrane for the release of the viral nucleocapsid proximal to the nucleus.
Trang 3with a cellular membrane results in content mixing and
the eventual release of the viral nucleocapsid and
tegu-ment proteins into the host cytoplasm (Figs 1 and 2)
Subsequently, symbolizing post-entry steps, HSV
nucleocapsids dissociate with tegument proteins and
bind a microtubule-dependent, minus end-directed
motor, dynein [9] Although most of the tegument
proteins are required for activation and modulation of
viral gene expression and shut-off of host protein
syn-thesis, some may participate in dynein-propelled
trans-port of the nucleocapsids along microtubules toward
the nuclear membrane for uncoating and the release of
viral DNA into the nucleus Transcription, replication
of viral DNA and assembly of progeny capsids take
place within the host nucleus The intricate details of
the steps required for entry are discussed below
Viral binding to filopodia
Although the virus binding to cells results from the
interaction of gC and⁄ or gB with heparan sulfate (HS),
the location on the cell where this binding can occur
the earliest has only recently been elucidated In human
conjunctival epithelial cells, virions were observed attaching to filopodia-like membrane protrusions [10]
It was also observed that virus attachment to filopodia was followed by unilateral movement of the virions towards the cell body Staining of many natural target cells with anti-HSV receptor antibodies indicated the expression of HS but not other entry receptors
Nectin-1 expression, for example, was limited to cell bodies with no detectable expression on filopodia (M J Oh &
D Shukla, unpublished results) The phenomenon of extracellular HSV-1 moving unilaterally towards the cell body on filopodia has also been observed in retinal pigment epithelial (RPE) and P19N neural cells [11,12]
A similar phenomenon for the transport of extracellular virus particles, termed ‘viral surfing’, has been observed with other viruses, such as retroviruses and human pap-illomavirus type-16 [13,14] Surfing is also shared by many additional herpesviruses, including cytomegalo-virus and human herpescytomegalo-virus-8 (V Tiwari & D Shukla, unpublished results)
Exposure of HSV-1 to cells can induce the forma-tion of filopodia [5] This presumably enhances the effi-cacy of viral infection by targeted delivery (via surfing)
of virus particles to cell bodies for subsequent fusion with plasma membrane or endocytosis [11] Based on functional analogy with retroviral surfing, it is quite likely that myosin-dependent F-actin retrograde flow is responsible for the HSV movements along filopodia [13] Ongoing studies demonstrate that filopodial bridges formed between two cells can help transfer extracellular HSV-1 virions from an infected to an uninfected cell (V Tiwari & D Shukla, unpublished results) Although viral trafficking on filopodia has not yet been observed or studied in HSV-2, because of strong similarities between HSV-1 and HSV-2 viral entry mechanisms, including the use of HS as an attachment receptor, it is quite possible that this phe-nomenon also plays a role in HSV-2 entry It is also worth noting that filopodia are not essential for virus attachment, with the latter occuring at virtually any place on the plasma membrane as long as receptors such as HS are present However, because of the pres-ence of filopodia at the leading edges of tissue layers (e.g in vivo during wound healing), they may provide easy ‘roadways’ for HSV to reach cell bodies for infection
Fusion at cell and vesicular membranes Fusion at the plasma membrane is a pH-independent process that requires gB, gD, gH and gL (Fig 2) [3] Cellular receptors such as nectin-1, HVEM or 3-OS
HS are also required The process is triggered by
gC
Attachment
HSV
gD
gD receptor
HS PG
Fusion
complex
Lipid mixing Content mixing
+
gD receptor
Fig 2 Molecular interactions that facilitate HSV entry Initial
attachment to cells is mediated by interaction between HSPGs
with HSV glycoproteins gC and ⁄ or gB Membrane fusion is
required for penetration of the viral nucleocapsid and tegument into
the cytoplasm Interaction between gD, gH-gL and a gD receptor
may be sufficient to bring conformational changes within gD to
trig-ger merging of viral and cellular membranes or lipid mixing
How-ever, a fourth glycoprotein, gB, is also required for complete fusion
and content mixing, which essentially results in the release of the
tegument and nucleocapsid into the cytoplasm A receptor for gB,
PILR-a, is also expected to play a role during the fusion process.
Its precise role is still emerging and therefore it is not shown here.
Trang 4conformational changes in gD that occur upon
recep-tor binding [15] Alteration in gD conformation then
mobilizes gH and gL, a heterodimer, and gB to initiate
the fusion process at the cell surface Multiprotein
complexes involving gD⁄ gB and gD ⁄ gH ⁄ gL have been
detected previously [9,16] and the entire complex
colo-calizes at the membrane during fusion [9] Although it
is expected that members of fusion active complex
undergo additional changes in conformation, no clear
information is available because of the lack of 3D
structures of any fusion active glycoprotein complex It
is, however, known that gD bound to its receptor
dem-onstrates significant changes in its conformation [15]
The HSV-induced membrane fusion is accomplished
by initially bringing the membranes of both the host
cell and virus into close contact by receptor⁄
glycopro-tein interactions followed by mixing of the membranes
or lipids to create an intermediary state, sometimes
referred as ‘hemifusion intermediate’ [17]
Subse-quently, a fusion pore is formed that allows mixing of
the cytoplasmic contents with viral contents, which, in
the case of HSV entry, essnetially implies the delivery
of viral tegument proteins and the nucleocapsid into
the cytoplasm Although gD and the gH⁄ gL
hetero-dimer may be sufficient to initiate the lipid mixing, a
full-scale fusion resulting in the mixing of the contents
requires the presence of gB as well (Fig 2) [17] It has
been suggested that fusion may require a sequential
action by the glycoproteins [17], although a recent
study did not find any pressing evidence for this [16]
Recent studies have implicated gB and gH as having
multiple fusogenic domains [4,6,18] gB is highly
con-served among herpesviruses; however, this glycoprotein
demonstrates a unique characteristic in HSV-1 and
HSV-2 because it remains uncleaved, whereas many
other herpesvirus gBs are post-translationally cleaved
Interestingly, both uncleaved and cleaved forms of gBs
share mixed features of class I and class II viral fusion
proteins, and thereby define a new, hybrid class of viral
fusogens [6] Class I proteins contain hairpin trimers
with N-terminal hydrophobic membrane-penetrating
peptides and centrally located a-helical coiled-coils By
contrast, class II proteins contain b-structures with
internally located fusion domains Similar to class I,
the gB trimer also contains a central a-helical core but
the fusion loops, similar to class II, are part of the
elongated b-hairpins The gB trimer shows strong
resemblance to fusogenic glycoprotein G of vesicular
stomatitis virus [6] Similar to gB, gH was also reported
to contain two heptad repeat regions that were found
to be necessary for fusion induction [4] Peptides that
corresponded to these regions effectively prevented
HSV-1 infection, suggesting the importance of these
regions The exact significance of gL is still unclear, although it is likely to play an important role by stabi-lizing or regulating gH conformations
The role of gD and its receptor in the fusion process
is likely that of a catalyst [3,15] Although gD does not contain any fusion peptides or domains, binding
to its receptor is required for the initiation of fusion, unless a poorly understood dependent but gD-receptor-independent pathway is initiated [19] It is worth noting that gD homologs are rare and, among human herpesviruses, only HSV-1 and HSV-2 appear
to express gD [1] It is unclear why HSV virions have evolved with a gD-based fusion trigger mechanism, whereas many other herpesviruses can do without it It
is conceivable that a gD-based mechanism may pro-vide some explanation for the differences in tissue tro-pism demonstrated by HSV-1 and HSV-2 It is also possible that the involvement of gD, which is capable
of interacting with at least three distinct classes of entry receptors, may enhance the host range for HSV because a vast majority of cultured cells of human or animal origin are susceptible to HSV entry Other human herpesviruses demonstrate more restrictive host ranges in terms of the cell lines that they infect [1] Although fusion at the plasma membrane was origi-nally considered to be the only route of viral entry for HSV, recent studies have supported the existence of an alternate entry mechanism utilizing endocytosis [4,5] This atypical endocytosis resembles phagocytosis in the virus uptake mechanism (Fig 1) [5] Under this pro-cess, the endocytosed enveloped particles subsequently fuse with a vesicular membrane Similar interactions between viral gB, gD, gH, gL and host cell receptors are expectedly mirrored within an intracellular vesicle such as an endosome to facilitate the fusion The gD receptor has been colocalized with endosomal markers and electron micrographs show the fusion and exit of nuclecapsids from the endosomes [5] Interestingly, unlike other bacterial and viral entry mechanisms, HSV-1 endocytosis does not appear to be mediated
by clathrin-coated pits or caveolae Furthermore, although endosomes provide an acidic background that may augment viral infectivity in certain cell types, this
pH dependency is not required in all cell lines [20–22] The choice between endocytosis and fusion at the plasma membrane appears to depend on individual cell types In Vero and Hep2 cells, fusion at the plasma membrane is the mechanism of choice; however, in cell types such as CHO, HeLa, RPE, human epidermal keratinocytes and human conjunctival epithelial cells, evidence of endocytosis of virions has been observed [11,21,22] Interestingly, gD was shown to down-regu-late nectin-1 in cells utilizing endocytosis in HSV-1
Trang 5entry, such as HeLa cells [23] By contrast, this
down-regulation was not seen in cells where HSV fuses at the
plasma membrane, such as in Vero cells This suggests
that the gD⁄ nectin-1 interaction is a possible factor in
the cell-type specific mode of HSV-1 internalization
Cellular receptors for gD
The main receptors that gD utilizes for cell entry are
nectin-1, nectin-2, HVEM and 3-O HS HSV-1 and
HSV-2 differ in their preference of gD receptor types
HVEM and nectin-1 are utilized by both virus types;
however, 3-O HS can only be used by HSV-1
Simi-larly, nectin-2 has not been shown to allow substantial
wild-type HSV-1 entry and it may have a greater effect
on HSV-2 entry [3]
Various cell types rely on different gD receptors for
HSV-1 entry: T lymphocytes and trabecular meshwork
cells utilize HVEM, whereas neuronal and epithelial
cells require nectin-1 [3,24] The dependence of HSV-2
on nectin-1 and HVEM for entry and spread in vivo
has been successfully demonstrated using single and
double knockout mice [25] 3-O HS, which is not a
receptor for HSV-2, appears to play a major role in
HSV-1 entry into primary cultures of corneal
fibro-blasts [26] Because 3-O HS is the most recently
dis-covered and perhaps least understood gD receptor, a
relatively detailed analysis of 3-O HS is presented
below
HS essentially represents a structurally diverse
family of polysaccharides sharing a common backbone
structure with varying degrees of additional modifica-tions and funcmodifica-tions [27] Demonstrating its structural complexity, HS, which is essentially a polymer of repeating disaccharide units containing a glucosamine and a glucuronic acid residue (Fig 3), is expressed in a variety of chain lengths with varying degrees of addi-tional modifications on the cell surfaces and extracellu-lar matrices of almost all cell types [27] It is an important attachment receptor shared by many patho-genic viruses, including all human herpesviruses except Epstein–Barr virus [1] The significance of HS is partic-ularly higher for HSV-1 because, in addition to attach-ment, it can also mediate membrane penetration by HSV-1 virions [7] Although a relatively less modified backbone HS chain can facilitate HSV attachment, a highly modified version, 3-OS HS, is required for interaction with gD and for independently triggering membrane fusion during entry and cell-to-cell spread processes [7,28,29] This fusion triggering 3-OS HS is generated after numerous modifications, including 2-O-, 6-O- and 3-O sulfations and epimerization (Fig 3) [7,27] The final step of parent chain sulfation to create 3-O HS is performed by a number of 3-O sulfotrans-ferase (3-OST) isoforms: 3-OST-1, -2, -3A, -3B, -4, -5 and -6 [1,30] Each isoform may demonstrate cell-type specific expression patterns and may produce a unique 3-OS HS chain with uniquely different functions 3-OST-1 creates 3-O HS with anti-thrombin binding sites and no gD binding activity, whereas other iso-forms such as 3-OST-2, -3, -4 and -6, create iso-forms of 3-O HS that can act as gD receptors [1,7,30] Their
6-O
S
6-O
S
6-O
S
6-O
S
6-O
S
6-O
S
6-O
3-O sulfotransferase 6-O sulfotransferase 2-O sulfotransferase
N-deacetylase, N-sulfotransferase, epimerase
S
6-O
S
3-O S
S N
S N S
N
2-O
S 2-O
S
N
S
2-O
S
N
S
N S
2-O
S
N
S
2-O
S
N
S
N multiple copies
= Glucose
= Galactose
= Xylose
= Serine
= α-linkage
= β-linkage
= Glucuronic acid
= Iduronic acid
S
2-O
S
N
S
2-O
S
2-O N
S
N
Fig 3 Outline of HS maturation A number
of listed enzymes participate in the modifi-cation of the parent chain HS, which is a polymer of repeating disaccharide units containing a glucosamine and a glucuronic acid residue All possible modifications and their preferred sequences (arrows) are shown.
Trang 6individual physiological functions are yet not well
known
A 3D structure for 3-O HS interaction with gD has
been suggested [15] The crystal structure of gD contains
a positively-charged deep pocket and a flat region with
clusters of basic amino acid residues Mutagenesis
stud-ies indicate that the pocket proximal to N-terminus may
have a role in 3-OS HS binding because mutations in
this region affect 3-OS HS usage by HSV-1 [31]
Inter-estingly, the same mutations also affect HVEM usage
but not nectin-1 Thus, it is quite possible that at least
one 3-OS HS binding region of gD may overlap with
HVEM binding sites [15,31] Efforts have also been
made to identify the structural specificity within HS that
is required for gD binding Apart from a 3-O sulfated
glucosamine, an upstream 2-O sulfated iduronic acid
appears to be required as well [7] To define the
mini-mum size requirement for this interaction, a 3-O sulfated
octasaccharide was generated [32] This octasaccharide
binds gD and demonstrates an inherent ability to block
gD-triggered cell-to-cell fusion Because the fusion was
blocked in cells that may or may not use 3-OS HS as the
major receptor for entry, it again suggests that 3-OS HS
binding sites may overlap with other receptor (HVEM
and possibly nectin-1) binding sites on gD Another
interesting property of 3-OS HS is that HSV-2 gD
fails to use it as a receptor for entry [7] Indeed, even for
the attachment process, HSV-2 demonstrates many
dif-ferences in the HS structure recognition than HSV-1
[33] It remains to be determined whether the inability to
use 3-OS HS and additional differences in HS binding
may have a role in the tissue tropism shown by the two
HSV serotypes
Paired immunoglobulin-like receptor
(PILR)-a: a new receptor for HSV-1 gB
Although viral attachment to cells shows strong
depen-dence on the interactions of gB and gC with HS,
stud-ies have also shown that cells without HS are still
susceptible to low-efficiency HSV-1 infection and
retain the ability to bind soluble gB [34,35] The
sug-gestion that there may be other receptors interacting
with gB was upheld by a recent finding regarding the
ability of gB to act as a ligand for PILR-a, one of the
paired inhibitory receptors found on monocytes,
mac-rophages and dendritic cells [19] CHO-K1 cells,
natu-rally resistant to HSV-1 infection, demonstrate
susceptibility to infection after transfection with
PILR-a plPILR-asmid HumPILR-an cell lines expressing both HVEM
and PILR-a experienced decreased viral entry when
antibodies to either PILR-a or HVEM were used,
supporting a separate need for both receptors
Additionally, PILR-a contains a tyrosine-based motif in its cytoplasmic domain that delivers inhibitory signals to the host cell Thus, by interacting with this inhibitory receptor, gB may allow HSV-1 to escape host immune system recognition via suppression at the same time exploiting the receptor for viral entry The most current notion with respect to HSV-induced membrane fusion may include PILR-a as an important and a balancing component of the fusion machinery [36] Interestingly, HSV-2 entry may not be so strongly dependent on PILR-a as a co-receptor, as shown for RPE cells [37] and further confirmed by additional findings [38]
HSV-1 binding mediated by PILR-a also appears to mediate cell–virus fusion at the plasma membrane Susceptible CHO cells utilize only endocytosis as an HSV entry mechanism However, after inhibiting endo-cytosis and transfecting CHO cells with PILR-a, HSV-1 entry was still possible, presumably at the plasma membrane [38] Therefore, PILR-a may be responsible for an alternate, but poorly understood, plasma membrane fusion mechanism of entry (Fig 2)
It is likely that PILR-a mediated fusion is a gD-depen-dent process that may not require a gD receptor as an essential component Most recent studies appear to indicate that PILR-a plays a role both in binding and fusion; however, additional studies are needed to better elucidate the importance of this receptor
Additional receptors for HSV entry
A few cell surface molecules have been implicated as putative HSV-1 gH receptors These include B5 and avb3 integrins [39,40] Expression cloning in porcine kidney cells resistant for HSV-1 entry has led to the discovery of B5, a type-2 membrane protein containing
an extracellular heptad repeat potentially capable of forming an a-helix for coiled-coils [39] Such structures may facilitate membrane fusion by interacting with viral proteins containing a-helices A synthetic peptide identical to the heptad repeat region blocks HSV infec-tion of B5-expressing porcine cells and human HEp-2 cells, suggesting a possible role for B5 in HSV-1 entry Because gH also contains an a-helix, it is speculated that it may be a ligand for B5 [39] No direct interac-tion between B5 and gH has been demonstrated to date By contrast, in a separate study, a soluble form
of gH-gL heterodimer was found to specifically bind cells expressing avb3 integrins, although the effect of this interaction on HSV-1 entry remains elusive [40] The binding appears to be highly specific because it can be prevented by mutating a potential integrin-binding motif, Arg-Gly-Asp (RGD), in gH Again,
Trang 7additional studies are needed to determine and
estab-lish the significance of potential gH receptors
Cell-to-cell viral spread
HSV-1 virus is also unique in that the spread of
infec-tion is not dependent on a hematogenous or lymphatic
route Cell-to-cell contact is essential in HSV-1 and
HSV-2 infection and elucidating the mechanism of
cell-to-cell spread is important for fully understanding
the overall viral infectious process Viral spread from
cell-to-cell depends on the same gD interaction with its
receptor(s) as that observed when free virions initially
infect a host cell [41] Interestingly, gE and gI are
glycoproteins that form a heterodimer required for
cell-to-cell spread but are not required for the initial
entry of free virus particles [42] During HSV infection,
the gE⁄ gI heterodimers move from the trans-Golgi
network to epithelial cell junctions along with other
viral glycoproteins and virion particles Removing the
gene responsible for the early sorting through the
TGN prevents subsequent cell-to-cell spread and
viri-ons are observed to travel towards the apical surface
instead of toward cell–cell junctions Assortment
through the TGN early in infection was found to be
necessary for efficient cell-to-cell spread of HSV-1 In
addition, gK has also been shown to play an essential
role in cell-to-cell spread in corneal and trigeminal
ganglia cells, comprising two cell lines that lead to the
more devastating consequences of diseases such as
blindness and meningitis [43] Mice infected with
mutated virus with the gene for gK deleted were
dem-onstrated a significantly decreased corneal spread and
also had decreased clinical signs of infection
Nectin-1 helps induce HSV-1 cell-to-cell spread
AF6, a multidomain protein that interacts with
nectin-1, leads to decreased cell-to-cell HSV-1 spread when
knocked down [44] Similar to nectin-1, this protein is
also involved in cell–cell adhesion and is found at cell
junctions Interestingly, AF6 knockdown does not
affect the nectin-1 clustering that occurs at junctions
for cell-to-cell transmission Therefore, nectin-1
recep-tor clustering appears to take place independently of
expression of the gene for AF6 and the clustering is
insufficient for cell-to-cell spread
Although 3-O HS has been shown to act as an gD
entry receptor for free virions fusing at the plasma
membrane, 3-O HS has also been shown to play a role
in cell-to-cell fusion and spread [28] Previously,
HVEM and nectin-1 expression were considered to be
required for cell-to-cell spread [41] However, more
recent studies imply that 3-O HS can also mediate
cell-to-cell fusion, which is required during spread Cells
expressing gB, gD, gH and gL, and therefore mimick-ing the essential viral machinery for membrane fusion, were able to fuse with 3-O HS-expressing cells [28] Importantly, these cells expressed neither nectin-1 nor HVEM When heparinase was used to eliminate the total HS, cell-to-cell fusion was dramatically decreased
In separate studies, it was found that 3-OS HS is cru-cial for the HSV-1-induced cell-to-cell fusion observed with primary cultures of corneal fibroblasts [45] Therefore, the role of 3-O HS may be more extensive with respect to the process of viral entry and spread than previously thought
Conclusions HSV-1 and HSV-2 both utilize similar mechanisms of binding, fusion and subsequent cell-to-cell spread Although fewer studies have been performed on HSV-2, the two viruses share 82% of their amino acid sequence and demonstrate high structural similarities [1] Therefore, many of the concepts developed for HSV-1 are likely to be applicable for HSV-2, although further confirmation is needed Different glycoproteins play unique key roles in the infectious cycle Binding can be initiated on filopodia with the interaction of gC and gB; however, subsequent fusion and penetration utilize gB, gD, gH and gL Entry can directly occur at the plasma membrane or via an intracellular vesicle Fusion appears to be mediated by fusogenic regions of
gB and gH triggered by the interaction of gD with its receptor Finally, cell-to-cell spread utilizes gD recep-tors, all fusion essential glycoproteins, the gE⁄ gI heterodimer and gK Clearly, significant advances have been made in our quest to understand the mechanism
of HSV entry, although many areas still remain poorly understood For example, the role of cellular signaling pathways is poorly defined Activation of Rho signaling has been demonstrated [5,46], as has the involvement
of focal adhesion kinases [47], although a complete picture is yet to emerge It is also unclear why the virus undergoes endocytosis when identical receptors can promote fusion at the plasma membrane A better understanding of how the virus fuses with a vesicular membrane is also needed For commonly spreading viruses such as HSV, such studies would have a high impact In lieu of a protective vaccine [2], efficient microbicides or prophylactics can be designed by devel-oping a better understanding of virus entry mecha-nisms Spread of the virus within a host can be contained by antiviral agents that target membrane fusion required for the spread Similarly, targeting shared entry phenomena, such as surfing, can lead to the production of broad-spectrum antiviral agents In
Trang 8summary, viral entry is an important area of research
with strong potential for identifying new strategies
aim-ing to prevent epidemic and pandemic viral diseases
caused by HSV, HIV, papilloma, influenza and many
additional viruses Continuing research in this area can
lead to a greater understanding of the disease process
and new treatments for viral diseases
Acknowledgements
The authors thank Dr Beatrice Yue (UIC) for critically
reading the manuscript The work described here that
was performed in the laboratory of D Shukla was
supported by a NIH RO1 grant AI057860 and a NIH
core grant EY01792 D.S is a recipient of Lew
Wasserman Merit award from Research to Prevent
Blindness, New York, NY, USA
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