molecular epidemiology of streptococcus agalactiae isolated from mastitis in brazilian dairy herds

Pleasecitethisarticleinpressas:Carvalho-CastroGA,etal.MolecularepidemiologyofStreptococcus agalactiaeisolatedfrommastitisinBrazilianIntroduction Escherichia coli, Klebsiella pneumoniae,

h tt p : / / w w w b j m i c r o b i o l c o m b r /

Rafael O Moreirab, Glaucia F Miana, Ingrid A Pradoa, Antônio Chalfun-Juniorb,

Geraldo M Costaa, ∗

aFederal University of Lavras, Laboratory of Bacteriology, Lavras, Brazil

bFederal University of Lavras, Central Laboratory of Molecular Biology, Lavras, Brazil

a r t i c l e i n f o

Article history:

Keywords:

a b s t r a c t

Streptococcus agalactiaeisoneofthemostcommonpathogensleadingtomastitisindairy

hylB, fbsA, fbsB,PI-1,PI-2a,andPI-2b,whichareassociatedwithadhesion,invasion,tissue

licenses/by-nc-nd/4.0/)

∗ Corresponding author at: Laboratory of Bacteriology, Department of Veterinary Medicine, Federal University of Lavras, Lavras,

http://dx.doi.org/10.1016/j.bjm.2017.02.004

Pleasecitethisarticleinpressas:Carvalho-CastroGA,etal.MolecularepidemiologyofStreptococcus agalactiaeisolatedfrommastitisinBrazilian

Introduction

Escherichia coli, Klebsiella pneumoniae, Streptococcus agalactiae,

Streptococcus uberis,andStaphylococcus aureus.2,4,5S agalactiae,

herds

Materials and methods

Bacterial strains

identi-fiedasS agalactiaephenotypicallybythecatalasetest,CAMP

dysgalactiaestrain(ATCC27957)wasusedasthenegative

assays

Specific PCR, molecular capsular typing and sequencing

S agalactiae classificationbyusingS agalactiae-specificPCR

Multilocus sequence typing and clonal group assignment

pheS, phenylalanyl transfer RNA synthetase; atr, amino

S agalactiae MLST database The eBURST V3 programme

(http://eburst.mlst.net)20 was then used to identify clonal

Genetic relationship of S agalactiae isolated from different

hosts in Brazil

Detection of virulence genes

Statistical and data analysis

Results

Table 1 presents all of the data generated during our

(Table1 InST-103,ST-146,andST-314,thefrequencydropped

study

S agalactiaestrainsinthisstudywereclusteredintotwomain

Pleasecitethisarticleinpressas:Carvalho-CastroGA,etal.MolecularepidemiologyofStreptococcus agalactiaeisolatedfrommastitisinBrazilian

Table 1 – Characteristics and frequency of 59 Group BStreptococcus(GBS) isolates listed according to capsular type,

sequence type, and clonal complex.

STa Allelicprofile No.ofisolates

inST(%)

Capsulartype(no.ofisolates) Mastitistype(no.ofisolates) Stateoforiginc

(no.ofisolates)

CCb

61 13,1,1,13,1,1,1 11(18.33%) III(9),Ia(1),NT(1) Clinical(3)/Subclinical(8) MG(7),SP(3),PE(1) 61

67 13,1,1,13,1,1,5 21(35.59%) Ia(1),II(12),III(3),IV(3),NT(2) Clinical(5)/Subclinical(16) MG(7),PE(6),SP(8) 67

91 25,1,1,13,15,1 9(15.25%) III(8),IV(1) Clinical(4)/Subclinical(5) MG(8),PE(1) 91

570 16,1,1,2,1,1,5 7(11.8%) Ib(5),IV(1),NT(1) Subclinical(7) MG(1),PR(6) 67

a ST:sequencetype

b CC:clonalcomplex

c States:MG,MinasGerais;SP,SãoPaulo;PE,Pernambuco;PR,Parana

Discussion

tobeS agalactiae.Thisresultwasnotcompletelyunexpected

CC67 (ST–67; ST–570)

CC314 (ST–226; ST–314)

CC64 (ST–61)

CC17 (ST–146)

CC103 (ST–103)

Singleton 91

ST–91

ST–67 ST–570 ST–256 ST–26 ST–2 ST–103 ST–226 ST–314 ST–553 ST–260 ST–552

CC 552

CC 314 CC103 CC19 CC26 CC67 CC17

CC64 ST–91

ST–146

66 70 93

47

100

100 100

71 90

inStreptococcusbacteria.29,40 Anotherpossiblecauseforthis

Pleasecitethisarticleinpressas:Carvalho-CastroGA,etal.MolecularepidemiologyofStreptococcus agalactiaeisolatedfrommastitisinBrazilian

pres-sure/diversity

hypothesis

ofS agalactiaewereevaluated,CC17andCC61wereclustered

sev-eralStreptococcusspecieshaveretaineddistinctcharacteristics

inS agalactiaestrainsisolatedfrom bovines.Although

S agalactiaeisolatedfromBrazilianbovines,whichisessential

pathogen

Funding

fellowship

Conflict of interest

Appendix A Supplementary data

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