isotopic evidence for residential mobility of farming communities during the transition to agriculture in britain

We use strontium and oxygen isotope analysis of tooth enamel from an Early Neolithic burial population in Gloucestershire, England, to evaluate the residence patterns of early farmers..

Research

Cite this article: Neil S, Evans J, Montgomery

J, Scarre C 2016 Isotopic evidence for

residential mobility of farming communities

during the transition to agriculture in Britain

R Soc open sci 3: 150522.

http://dx.doi.org/10.1098/rsos.150522

Received: 29 September 2015

Accepted: 11 December 2015

Subject Category:

Earth science

Subject Areas:

environmental science/ecology/evolution

Keywords:

development of agriculture, Neolithic,

sedentism, mobility, strontium, isotope

analysis

Author for correspondence:

Samantha Neil

e-mail:s.a.neil@durham.ac.uk

Isotopic evidence for residential mobility of farming communities during the transition to agriculture in Britain

Samantha Neil 1 , Jane Evans 2 , Janet Montgomery 1 and Chris Scarre 1

1Department of Archaeology, Durham University, South Road, Durham DH1 3LE, UK

2NERC Isotope Geosciences Laboratory, Keyworth, Nottingham NG12 5GG, UK

Development of agriculture is often assumed to be accompanied by a decline in residential mobility, and sedentism is frequently proposed to provide the basis for economic intensification, population growth and increasing social complexity In Britain, however, the nature of the

agricultural transition (ca 4000 BC) and its effect on residence

patterns has been intensely debated Some authors attribute the transition to the arrival of populations who practised a system

of sedentary intensive mixed farming similar to that of the very

earliest agricultural regimes in central Europe, ca 5500 BC, with

cultivation of crops in fixed plots and livestock keeping close

to permanently occupied farmsteads Others argue that local hunter–gatherers within Britain adopted selected elements

of a farming economy and retained a mobile way of life We use strontium and oxygen isotope analysis of tooth enamel from an Early Neolithic burial population in Gloucestershire, England, to evaluate the residence patterns of early farmers Our results are consistent with the hypothesis that early farming communities in Britain were residentially mobile and were not fully sedentary Results highlight the diverse nature of settlement strategies associated with early farming in Europe and are of wider significance to understanding the effect of the transition to agriculture on residence patterns

1 Introduction The transition from hunting and gathering to farming is often considered to be accompanied by a decline in residential mobility

as sedentism is assumed to facilitate economic intensification, leading to population expansion and the development

2016 The Authors Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited

of complex societies (e.g [1 3] The agricultural transition in Britain (ca 4000–3500 BC) is marked by

the importation of non-native species of domesticated animals from continental Europe, evidence for cereal cultivation and the appearance of new traditions of pottery manufacturing, lithic technologies and monument construction However, both the processes that facilitated the transition and the nature of the first farming systems associated with it remain intensely debated (e.g [4 8]) Some authors attribute development of farming in Britain to the arrival of settled agriculturalists from continental Europe who practised a similar system of intensive mixed agriculture to that of the Linearbandkeramik (LBK), the first farming systems which developed in central Europe from approximately 5500 BC [9 11] Arable production is proposed to have been closely integrated with livestock keeping: cultivation is suggested

to have taken place in fixed plots with animals being kept close to permanently occupied farmsteads [10,

12,13] Archaeobotanical evidence is considered to rule out shifting cultivation, and agricultural regimes

in Early Neolithic Britain are proposed to have been similar to those of the LBK [14] Cereals are argued

to have been a dietary staple [15–17] and, due to the demands of cultivation, it is suggested that the first agriculturalists in Britain were fully sedentary [18,19]

In contrast, other authors argue that unlike the very first agriculturalists in central Europe, early farmers in Britain were not fully sedentary and farming regimes were based on agro-pastoralism [20–22] These authors suggest that the number of substantial timber buildings so far discovered that date to this period is limited, and question the interpretation that they functioned as permanently occupied farmsteads [23] Rather than a fully arable economy, subsistence practice is instead proposed to have been predicated on intensive dairying [24–29] and routine exploitation of wild plant species as well

as cereals [30–33] This is in turn considered by some authors to demonstrate that the transition to agriculture occurred through adoption of selected elements of a farming economy by local Mesolithic populations, who retained a mobile way of life thought to be characteristic of hunter–gatherers [8,34,35] Recent analysis of temporal changes in the robusticity of lower limb bones is also considered to support continued residential mobility during the Neolithic and a gradual, rather than abrupt, transition to sedentism [36] In Britain, Early Neolithic occupation evidence frequently comprises pits, stakeholes, lithic scatters and middens which are interpreted as the remains of temporary camps that were occupied episodically (e.g [37–39]) Rather than sedentism, it is therefore suggested that residence patterns were based on ‘tethered mobility’ [40,41], a system of cyclical transhumance in which communities repeatedly moved between favoured occupation sites

In view of these debates, we applied strontium and oxygen isotope analysis of tooth enamel to evaluate the land use and residence patterns of the first farmers in Britain Strontium isotope analysis

of tooth enamel is a robust and highly reliable technique that is routinely used for geographical provenancing (e.g [42,43]) Strontium (87Sr/86Sr) isotope ratios vary with the age and composition of bedrock [44] Strontium weathers from rocks into soils where it becomes available to plants and enters the human food chain [45] Tooth enamel is highly resistant to diagenesis (e.g [46,47]) and as mass-dependent fractionation does not affect conventionally measured87Sr/86Sr values [48], strontium isotope ratios in enamel directly reflect the location from which an individual obtained food during the period in which a tooth was mineralizing [49,50] Comparison of87Sr/86Sr values in teeth that form at successive stages of childhood to mapped values in modern vegetation and water (e.g [51,52]) can therefore be used

to evaluate whether an individual was residentially mobile

The oxygen isotope composition of water also varies geographically with factors such as temperature, latitude and altitude (e.g [53,54]) In Britain,δ18O values of contemporary groundwaters are primarily influenced by rainfall; western Britain receives higher rainfall, and therefore groundwaters have higherδ18O values than those in eastern Britain [55] A statistically significant difference in the mean

δ18Ophosphate values measured in tooth enamel of multi-period archaeological populations buried in western Britain (18.2 ± 1, 2σ ) from those in eastern Britain (17.2 ± 1.3, 2σ ) is considered to reflect the underlying geographical variation in the oxygen isotope composition of local drinking water between the two areas [56] It is argued that occupation of these different regions of Britain is associated with 95% ranges of 17.2 to 19.2 and 15.9 to 18.5, respectively (ibid.) These ranges were determined using isotope analysis of the phosphate (PO4 −) fraction of tooth enamel However, the carbonate (CO3 −) fraction is equally suitable for analysis and, as theδ18O values in theδ18Ophosphate andδ18Ocarbonate fractions are considered to be well correlated, conversion between the two can be undertaken using the

equation developed by Chenery et al [57] (see Material and methods) Interpretation ofδ18O results must, however, give consideration to the potential influence of culturally mediated behaviour, such as culinary practice (e.g stewing foods and brewing) [58] or consumption of fluids that have undergone fractionation through biological processes (e.g breast milk or cow’s milk) [59–65] on the oxygen isotope composition of ingested fluids

Isotope analysis of the structural carbonate fraction of enamel simultaneously yields carbon isotope ratios (δ13Ccarbonate) which provide additional dietary information The use of carbon isotope analysis for this purpose exploits the large variation in natural abundance of δ13C between plants that use the two dominant (C3 or C4) photosynthetic pathways during fixation of CO2 energy and variation

inδ13C values between terrestrial C3 and marine ecosystems (e.g [66,67]) Current understanding of dietary composition in the European Neolithic is based on analysis of δ13C andδ15N values in bone collagen, which predominantly reflect the protein component of the diet and support the exploitation

of C3 terrestrial sources of protein during the Early Neolithic in Britain (e.g [68–70]) In contrast,

δ13Ccarbonatevalues in bioapatite reflect the isotope composition of the diet as a whole, including lipids and carbohydrates [71,72] Individuals who obtain all of their diet from C3 terrestrial sources may be predicted to haveδ13Ccarbonatevalues between approximately−17.0 to −14.0 [73,74]

Thirty-eight teeth, including the consecutively mineralizing molars of 18 different individuals, were analysed to obtain strontium, oxygen and carbon isotope ratios (see Material and methods and

table 1) The sampled population derives from Hazleton North long cairn, one of the few Early Neolithic monuments in Britain which has been completely excavated to modern standards [75,76] The monument, which is estimated to have been constructed between 3710 and 3655 cal BC and used for burial over at least two to three generations (95% probability, OXCAL v 3.5) [77], is situated in the Cotswold region of England (figure 1) In addition to analyses undertaken on individuals buried

in chambers on the north and south sides of the monument, a tooth from a small scatter of human remains found stratified underneath the long cairn that is considered to represent the earliest dated burial activity at the site (ibid.) was also analysed The presence of a hearth, post-holes, a midden and evidence for cultivation directly beneath the monument are argued to indicate that it was constructed

at a site previously used for occupation [76,79] Samples of enamel from the three main domesticated species found in the midden (cattle, sheep/goat and pig) [80] were also taken for analysis

The lithology at the site and in the surrounding Cotswold region of Gloucestershire is composed of Oolitic limestone [81,82] a marine carbonate rock which has an87Sr/86Sr value equivalent to seawater in the Middle Jurassic period (0.7068–0.7073) [83] However, the bioavailable87Sr/86Sr range on Oolitic limestone is also influenced by rainwater [84] In an island facing the Atlantic such as Britain, the value of rainwater is close to that of seawater, which throughout the Holocene has had a ratio close

to 0.7092 [83,85] Due to the combination of these two sources of strontium, samples from plants and waters on Oolitic limestones in the Cotswolds give87Sr/86Sr values between 0.7076 and 0.7092, with a mean of 0.7086± 0.0004 (1σ , n = 17) [52,78,86] A sedentary self-sufficient population subsisting solely on resources obtained from a homogeneous lithological unit such as Oolitic limestone would be predicted

to plot on a diagonal mixing array between two sources of dietary strontium (end-members): the ratio bioavailable on that lithological unit and that of rainwater [42,87] Had the sampled population derived all their resources locally, cultivating fields and keeping herds of animals around a permanently occupied settlement at the site or in the surrounding Cotswold region of England, they would be expected to plot between the minimum value bioavailable on Oolitic limestone (0.7076) and rainwater (∼0.7092)

2 Material and methods

2.1 Sample selection

The human burial assemblage from Hazleton North consists of disarticulated and co-mingled human remains Care was therefore taken to avoid the potential for duplication of isotope results through inadvertent sampling of antimeres which could belong to the same individual Teeth that remained

in situ in left-sided mandibular fragments were therefore selected for sampling Teeth in right-sided

mandibular fragments were not sampled unless the refitting left-hand side of the dentition was present Maxillary teeth were only used if the re-fitting mandible belonging to the individual was present In total, 18 different individuals (14 adults and four pre-adults) were sampled In addition to the 18 discrete individuals sampled by this project, two chips of core enamel taken during sampling of maxillary dentition by a project unrelated to this study were also analysed to obtain isotope ratios: 4786 (LM2) and 10 494 (LM3) Like the human assemblage, the pre-cairn animal assemblage from Hazleton North

is also highly fragmentary Cranial remains are dominated by loose teeth which cannot be assigned to specific individuals [80] One tooth from each of the three main domesticated species (cattle, sheep/goat and pig) present in the pre-cairn assemblage (ibid.) was sampled in order to compare87Sr/86Sr values with those of the human group

Ta bl e Str on tium isot ope ra ,s tr tium co nc en tr ions and δ 18Ocarbona te and δ 13Ccarbona te va lues in enamel of humans and animals fr Hazlet on N th Appr ox ima te age at dea th is based on tooth eruption aft er Rogers [ ,p p 190–191]; L= lef t, righ t; first ,sec ond and thir d tm olar teeth ar designa ted as M1, M and M3, respec tiv ely; sec ond permanen tp re teeth ar des igna ted as PM2; first mandibular cen tr permanen ti teeth designa ted as LI1 co ntex t/ Sr pp m δ 13 Ccarbona te δ 18 Ocarbona te δ 18 Ocarbona te δ 18 Ophospha te sample number loca tion bo x age at dea th tooth 87Sr/ 86Sr (mg kg − 1)  VPDB  VPDB  VSMO W  VSMO W 10 414/individual G nor th chamber basal fill 336 3–4 years mandibular LM1 0.7 1027 54 − 16.0 − 3.8 27.0 18.2

10494 south chamber fill 412 adult maxillar y 0.70963 49 − 15.1 − 4.0 26.8 18.0

11456 south chamber fill 412 adult m LM2 0.7 1036 67 − 15.2 − 4.2 26.6 17.8

mandibular LM3 0.7 1016 58 − 16.7 − 4.4 26.4 17.6

11 903 pr cairn; SW quad ce ll 211 unkno w loose pr emolar 0.70866 45 − 16.0 − 3.5 27.3 18.5

12527 south chamber 453 6–9 ye ars m RM1 0.70833 62 − 16.1 − 2.5 28.3 19.5

35 96 south en tr e 354 adult mandibular RPM2 0.70853 47 − 16.1 − 3.8 27.0 18.2

3793 south en tr e 354 adult mandibular RM1 0.70818 63 − 14.9 − 3.1 27.8 19.0

mandibular RM2 0.70818 44 − 14.7 − 3.8 27.0 18.2

mandibular RM3 0.7 1033 101 − 15.7 − 3.1 27.7 18.9

3831 south en tr e 354 adult mandibular RM2 0.7 1036 144 − 15.0 − 4.0 26.8 18.0

mandibular RM3 0.70838 35 − 16.3 − 3.8 27.0 18.2

407 7/416 9 south en tr e 354 adult mandibular RM1 0.70835 37 − 16.0 − 2.9 28.0 19.2

mandibular RM2 0.70827 30 − 15.5 − 3.7 27.1 18.3

mandibular RM3 0.70806 24 − 16.3 − 4.2 26.6 17.8

4118 nor th en tr e 267 adult mandibular LI1 0.7 1027 65 − 15.4 − 3.5 27.3 18.5

4786 south chamber passage 187 adult m y 0.7083 9 48 − 14.7 − 3.1 27.7 18.9

4806/7387 south chamber passage 323 adult m LM1 0.70887 39 − 16.4 − 3.0 27.8 19.1

Ta bl e (C ued ) co ntex t/ Sr pp m δ 13 Ccarbona te δ 18 Ocarbona te δ 18 Ocarbona te δ 18 Ophospha te sample number loca tion bo x age at dea th tooth 87Sr/ 86Sr (mg kg − 1)  VPDB  VPDB  VSMO W  VSMO W

5037/Sk elet on 1 nor th en tr e 267 adult m axillar y 0.70797 22 − 16.5 − 3.9 26.9 18.1

maxillar y 0.70804 22 − 16.0 − 3.7 27.1 18.3

maxillar y 0.70825 27 − 15.9 − 3.8 27.0 18.1

5880 nor th chamber basal fill 336 adult m andibular LM1 0.70957 85 − 15.2 − 3.2 27.6 18.8

mandibular LM2 0.70912 52 − 15.8 − 3.2 27.6 18.8

mandibular LM3 0.70888 40 − 16.1 − 3.8 27.0 18.2

7386/6815 south chamber passage 323 adult m andibular LPM2 0.70838 45 − 15.5 − 4.4 26.4 17.6

7656 south chamber passage 323 adult m andibular RM1 0.70794 41 − 15.2 − 3.0 27.2 19.1

mandibular RM2 0.70855 55 − 14.5 − 3.6 27.2 18.4

mandibular RM3 0.70813 32 − 16.4 − 3.6 27.2 18.4

8701/individual E south chamber fill 412 12–15 years mandibular RM1 0.70807 40 − 16.1 − 3.9 26.9 18.1

8751 south chamber fill 412 adult m andibular LM2 0.70804 37 − 15.8 − 3.7 27.1 18.2

mandibular LM3 0.7 1066 84 − 16.0 − 3.3 27.5 18.7

897 4 south en tr e 353 adult m andibular LM2 0.70962 76 − 15.9 − 4.4 26.4 17.6

mandibular LM3 0.7 1120 88 − 15.6 − 4.0 26.8 18.0

9025 nor th chamber fill 435 adult m andibular LM1 0.7 1262 74 − 15.6 − 3.2 27.6 18.8

9951 south chamber fill 412 9–10 ye ars m andibular LM1 0.70810 62 − 15.5 − 4.0 26.8 18.0

HBG H 37 4 w pr cairn/NW quad ce ll 211/bo x unkno wn loose m tooth 0.7 105 9 180

HBG H 065 pig pr cairn/NW quad ce ll 211/bo x unkno wn maxillar y 0.707 74 82

HBG H 304 sheep/goa t pr cairn/SW quad cell S 211/bo x unkno wn loose m tooth 0.70821 216

Devonian

Silurian

Ordovician

Upper Devonian Rocks Lower Devonian mudstones and sandstones Lower Devonian sandstones and conglomerate

Pridoli rocks Ludlow rocks Llandovery rocks

Tremadoc rocks

Jurassic

Triassic

Grey Chalk White Chalk Gault Formation and Upper Greensand

South Wales Upper Coal Measures Dinantian Rocks

Lower Greensand

Corallian Group Upper Jurassic mudstones and sandstones Kellaways Formation and Oxford Clay Great Oolite

Inferior Oolite Lias Group

Triassic mudstones, siltstones and sandstones Triassic sandstones and conglomerate

N

20 km 0

Figure 1 Map of bedrock geology illustrating sites and locations discussed in the text Based on British Geological Survey and Ordnance

Survey map data, reproduced with permission of the British Geological Survey and Ordnance Survey, c NERC/Crown copyright 2015 Bioavailable87Sr/86Sr ranges associated with different lithologies are based on measured values by Evans et al [51], Warham [52] and

Chenery et al [78]

Due to the fragmentary nature of the assemblage and disarticulation of cranial remains from other skeletal elements, the sex of the majority of sampled individuals cannot be stated with confidence Only one individual, Skeleton 1, was found in a virtually complete fully articulated state and may be sexed

as male [75] Where available, information on the approximate age of the individuals, as determined by dental eruption, is provided intable 1, after Rogers [75, pp 190–191] Wherever present, consecutively mineralizing molar teeth were selected in order to examine the variability in isotope ratios between teeth that form at different stages of childhood Development of the crown of the first permanent adult molar

commences in utero, just prior to birth, and completes by approximately 4.5± 0.5 years of age, while the second molar crown forms between 2.5± 0.5 years and 8.5 ± 0.5 years of age [88,89] The timing of third molar formation is most variable [90], with initial cusp formation taking place at approximately 8.5± 0.5 years and crown completion by 14.5 ± 0.5 years [88] Strontium and oxygen isotope analysis

was conducted on samples of bulk enamel, and isotope ratios therefore represent the weighted average

of the sources to which the individual was exposed during the period the tooth was mineralizing As the process of enamel formation is highly complex (e.g [89]), and as strontium may have an extended residence time within the body prior to its incorporation in enamel [42], it is currently uncertain that greater chronological resolution can be achieved by serial sampling of human tooth enamel

2.2 Sample preparation and laboratory analysis

Teeth were processed following procedures developed by Montgomery [49] Surface enamel was thoroughly abraded using a tungsten carbide dental burr Enamel chips were then cut using a flexible diamond-edged rotary saw and surfaces again mechanically cleaned using a tungsten carbide dental burr to remove any adhering dentine An enamel chip of approximately 20–30 mg in weight from each tooth was taken for strontium isotope analysis and of approximately 10 mg in weight for oxygen isotope analysis Dental saws and burrs were cleaned ultrasonically for 5 min and rinsed three times in high purity de-ionized water between preparation of samples

2.3 87 Sr/ 86 Sr analysis

Samples were transferred in clean sealed containers to the Class 100, HEPA-filtered laboratory facilities

at the Natural Environment Research Council Isotope Geosciences Laboratory (Keyworth, Nottingham, UK) Enamel chips were cleaned ultrasonically and rinsed in high purity water (Millipore Alpha Q) They were then dried, weighed into pre-cleaned Teflon beakers and spiked with a known amount of

84Sr tracer solution to obtain strontium concentrations Each sample was dissolved in Teflon distilled

8 M HNO3 Samples were converted to chloride using 6 M HCl, taken up in titrated 2.5 M HCl and pipetted onto ion-exchange chromatography columns Strontium was separated with Dowex (AG50-R X8) resin (200–400 mesh) Procedural blanks were below 150 pg Samples were loaded on to Re filaments using a method adapted from Birck [91] Strontium isotope composition and concentrations were then determined by thermal ionization mass spectroscopy using a ThermoTriton automated multi-collector mass spectrometer To correct for fractionation during the process of mass spectrometry,87Sr/86Sr values are normalized to the accepted value for88Sr/86Sr= 0.1194 During the period of this study, the machine gave a value for the international standard for87Sr/86Sr (NBS 987) of 0.710253± 0.000012 (2σ , n = 350).

An estimate of the reproducibility of strontium concentration (Sr ppm) is provided by replicate analysis

of an aliquot of bone standard solution (NIST1486), which gave 7.22± 0.27 ppm (±3.75%, 1σ , n = 16).

2.4 δ 18 O and δ 13 C analysis

Initial preparation of core enamel chips for δ18O andδ13C analysis was undertaken using the same methods employed above for strontium isotope analysis Samples were then transferred as clean core enamel chips to the Natural Environment Research Council Isotope Geosciences Laboratory where they were powdered Oxygen (δ18Ocarbonate) and carbon (δ13Ccarbonate) isotope ratios in the carbonate fraction

of enamel were determined using approximately 3 mg of clean powdered enamel following the method

outlined in Chenery et al [57] Isotope ratios are reported as delta (δ) values, in parts per thousand (per mil;) normalized to the VPDB scale using an in-house carbonate reference material, Keyworth Carrera marble (KCM), which is calibrated against NBS19 certified reference material Analytical reproducibility for this run of KCM was±0.09 (1σ , n = 14) for δ18O and forδ13C± 0.04 (1σ , n = 14) δ18Ocarbonate values were normalized to the VSMOW scale using the equation of Coplen [92] (VSMOW= 1.03091 ×

δ18O VPDB + 30.91) Conversion between δ18Ocarbonate toδ18Ophosphate was then undertaken using

the regression equation of Chenery et al [57] (δ18Ophosphate= 1.0322 × δ18Ocarbonate− 9.6849) The error involved in calculatingδ18Ophosphateis considered to be low (0.28, 1σ , ibid.)

3 Results

The majority of the population plot on a diagonal array in which87Sr/86Sr increases with elemental concentration between a lower value less than 0.7085 and an upper value more than 0.7105 Adjacent molar teeth of individuals in the group also exhibit a shift in strontium isotope ratio and concentration between a lower value less than 0.7085 and an upper value more than 0.7105, or vice versa (figure 2) Isotope ratios do not vary with burial context: individuals from chambers on both the north and south side of the monument plot on the same array Only one individual appears to be an outlier from the

= first molar

= second molar

= third molar

= premolar / incisor

0.7130

0.7125

0.7120

0.7115

0.7110

0.7105

0.7100

0.7095

0.7090

0.7085

0.7080

0.7075

0.7070

pig sheep/goat

cow

local biosphere range

1/Sr ppm × 1000

86Sr

delineate the approximate87Sr/86Sr biosphere range available on Oolitic limestone Light green symbols indicate individuals who can be interpreted as sedentary and red symbols denote the rest of the population Tooth types are denoted by the key in the upper right of the diagram Cow, sheep/goat and pig labelled within the diagram are from pre-cairn contexts 2σ errors for87

Sr/86Sr are within the symbol

strontium isotope array with a value higher than 0.7125 Three individuals have an87Sr/86Sr value that

is consistent with the local biosphere87Sr/86Sr range on all three of their consecutively mineralizing molar teeth (highlighted in light green,figure 2)

δ18Ocarbonatevalues range between 26.4 and 28.3, with a mean of 27.1 ± 0.4 (n = 35, 1σ ) With the exception of first molar teeth, which more frequently exhibit oxygen isotope ratios higher than 27.5 (figure 3), the majority of teeth with87Sr/86Sr values near to 0.7085 haveδ18Ocarbonatevalues that plot in

a cluster close to 27.0 In contrast, teeth with higher strontium isotope ratios (more than 0.7105) that plot above the local biosphere range exhibit a less constrained range ofδ18Ocarbonatevalues.δ13Ccarbonate values of the sampled human population range between−16.6 and −14.5 (mean 15.6 ± 0.5, n = 35,

1σ ;table 1) and therefore fall within the range of values expected for a diet dominated by C3terrestrial sources Animals sampled from the pre-cairn contexts (figure 3) exhibit a comparable range of87Sr/86Sr values to the human group While the sheep/goat and pig have strontium isotope ratios comparable to the local biosphere range, the cow has a value which is higher than 0.7105 The herbivores that were sampled exhibit higher strontium concentrations than the human population This is consistent with the progressive discrimination against strontium which results from bio-purification of calcium with increasing trophic level within a food chain (e.g [93,94])

4 Discussion

A sedentary self-sufficient population subsisting solely on resources obtained from a homogeneous lithological unit such as Oolitic limestone would be predicted to plot on a diagonal mixing array between two sources of dietary strontium (end-members), the ratio bioavailable on that lithological unit and that

of rainwater [42,87] The majority of individuals do plot on a diagonal array, which indicates that they derived dietary strontium from two dominant sources (dietary end-members) that were incorporated in differing proportions during tooth mineralization [ibid.] However, the strontium isotope array does not conform to that predicted for a sedentary self-sufficient population who had subsisted solely on locally bioavailable resources One of the two dietary sources exploited by the group had a87Sr/86Sr value close to 0.7085 and is therefore comparable to the local87Sr/86Sr biosphere range However, the other dietary end-member (more than 0.7105) is not [51,52,78] In southern Britain lithologies that routinely give measured biosphere87Sr/86Sr values below 0.7085 are geographically separated from those that

28.5 28.0

27.5 27.0

26.5 26.0

0.7130

0.7125

0.7120

0.7115

0.7110

0.7105

0.7100

0.7095

0.7090

0.7085

0.7080

0.7075

0.7070

87Sr

= first molar

= second molar

= third molar

= premolar / incisor

have87Sr/86Sr values that are comparable to the local biosphere range andδ18Ocarbonatevalues that cluster close to 27.0.Toothtypesare illustrated within the key in the upper right of the diagram 2σ errors for87

Sr/86Sr are within the symbol Analytical error forδ18

Ocarbonate

is shown as±0.2 (2σ ).

give values higher than 0.7105, with the exception of the Lizard Peninsula in Cornwall where a small area of serpentinite crops out next to Devonian rocks, approximately 300 km away from Hazleton North [51] With the exception of the latter area, all current measured 87Sr/86Sr biosphere values suggest that strontium isotope ratios below 0.7085 and values above 0.7105 are not routinely bioavailable in close proximity in southern Britain Therefore, to generate the array seen in figure 2, a population who inhabited southern Britain would need to have sourced their diet from at least two different geographical locations In the absence of any evidence for a market economy during this period to suggest communities derived a significant component of their diet through trade, the strontium isotope array is consistent with movement of individuals between different localities to obtain dietary resources The closest proximal area to the site where87Sr/86Sr values above 0.7105 are routinely bioavailable is more than 40 km to the west or southwest Plants and waters on lithologies of Carboniferous, Devonian

or Silurian age in areas of southwestern Britain such as Gloucestershire, Herefordshire or Worcestershire routinely give values higher than 0.7105, although areas further afield, for example in Wales or Somerset, cannot be excluded [51,78] The interpretation that the group routinely derived dietary strontium from at least two separate locations is also supported by strontium isotope results from adjacent molar teeth which plot on the same strontium isotope array Several individuals in the group exhibit a shift in

87Sr/86Sr values from the upper (more than 0.7105) to the lower end-member (less than 0.7085), or vice versa (illustrated by arrows infigure 2) This shift in values between consecutively mineralizing molar teeth is consistent with regular movement backward and forward between at least two different geographical locations

δ18Ocarbonateresults may also support the interpretation that the group derived their diet from more than one location The majority of teeth with87Sr/86Sr values that are comparable to the local biosphere range haveδ18Ocarbonatevalues that plot in a cluster close to 27.0 Individuals who plot within this cluster appear to have derived ingested fluids from a source which conferred a very similar oxygen isotope value Deviation in values from the cluster, which appears to represent one of the dietary sources exploited by the sampled group, could be a consequence of localized variation in the oxygen isotope composition of groundwaters between the different geographical locations used by the population Adjacent molar teeth of different individuals within the sampled group exhibit a shift in oxygen isotope values backward and forward, into and out of this cluster, with those teeth that have higher strontium isotope ratios (more than 0.7105) being associated with a less constrained range ofδ18Ocarbonatevalues

First molar teeth, which begin to form just prior to birth [88,89], more frequently plot withδ18Ocarbonate values that are higher than 27.5 (figure 3) and it is possible that values within these teeth may be influenced by consumption of breast milk, which has a higherδ18O value relative to meteoric water as

a result of the metabolic fractionation that occurs in the mother’s body [63–65] The meanδ18Ophosphate value of second and third molar teeth 18.2± 0.4 (n = 20, 1σ ) is, however, comparable to that which has been proposed by Evans et al [56] to represent occupation of the western side of Britain (18.2 ± 1,

2σ ) and could support the interpretation that the group routinely moved around lithologies within

this region

The majority of the population, both adults and children of different ages at death, and consecutively mineralizing molars of different individuals, have87Sr/86Sr values which conform to the same strontium isotope array As such it is highly likely that the sampled group participated in a very similar residential routine throughout the period to which the burials are dated, over at least two to three generations during the thirty-seventh century BC [77] The tooth from pre-cairn contexts (table 1) plots within the cluster of individuals who have teeth with87Sr/86Sr values comparable to the local biosphere range and

δ18Ocarbonatevalues close to 27.0.This individual could therefore have derived their diet from one of the locations that was exploited by the population who were buried within the cairn The87Sr/86Sr value of the lower dietary end-member exploited by the human burial population (less than 0.7085) is consistent with the local bioavailable range and, in conjunction with the presence of a hearth, midden and evidence for cultivation beneath the monument [76,77], supports the hypothesis that the site itself was one of the two locations occupied during childhood and adolescence by the population who were subsequently buried within the cairn Occupation of other areas in southern Britain, such as the Cotswolds, or Cretaceous Chalk, which afford a similar bioavailable range [51], cannot be excluded (figure 1) However, the inference that the site itself may have been of significance within the residential tradition of the group who were later buried in the cairn is further supported by the presence of fragments of worked quartzitic sandstone found in pre-cairn contexts These fragments were imported to the site from at least 40 km away They derive from lithologies of Carboniferous or older age [82] which routinely give bioavailable

87Sr/86Sr values comparable to those that provided the upper end-member (more than 0.7105) for the group buried in the cairn [51,78] Strontium isotope ratios of animals in the midden below the cairn also appear to reflect the residential regime of the human population in the cairn The animals in the midden were raised at more than one geographical location They have87Sr/86Sr values that are comparable to the upper and lower dietary end-members exploited by the human group and thus are consistent with those of the two areas used by the population who were buried at the site

Only three individuals in the sampled human population (highlighted in light green,figure 2) possess values below 0.7085 on each of their consecutively mineralizing molar teeth This could be consistent with sedentism Although it is possible that these individuals moved between different areas which afford the same87Sr/86Sr bioavailable range (e.g between Oolitic limestone and Cretaceous Chalk), the presence of similar values on adjacent molar teeth may support the interpretation that they occupied one of the locations exploited by the group for a longer period during their early life Unlike these individuals, the majority of the sampled population do not exhibit values that are consistent with permanent occupation of the same location during early life, or with ‘radial mobility’ [11], brief visits

to temporary outlying camps from a single permanent settlement Ratios in bulk enamel represent the weighted average of all sources of strontium to which the individual had been exposed during the period the tooth was mineralizing [42] To gain a value higher than87Sr/86Sr 0.7105, an individual would need

to have derived a significant part of their diet from an area of radiogenic geology, more than would be obtained by a brief visit away from an area with a value below 0.7085 In addition, the regular shift in values exhibited by individuals between adjacent molar teeth, from 0.7105 to 0.7085 or vice versa, is also consistent with a change in location between the two areas used by the group The possibility that the array seen infigure 2represents a migrant population who had been fully sedentary at a distant location, for example on the continent, where lithologies that provided biosphere values above 0.7085 and below 0.7105 cropped out close together (i.e within the same field system), should also be considered However, evidence for cultivation and occupation beneath the cairn [76,79] in conjunction with evidence for the sourcing of artefacts (above) and strontium isotope results from animals in pre-cairn contexts support the hypothesis that the location at which the cairn was constructed was of pre-existing importance within the residential tradition of a group who inhabited southern Britain

Results therefore support the model of ‘tethered mobility’ proposed by Whittle [40,41, p 21, 43], a settlement system in which individuals repeatedly moved between favoured occupation sites Strontium isotope ratios in tooth enamel are a reflection of sources to which people were exposed during early life and as such the results could be compatible with routine movement of individuals during childhood