glial and tissue specific regulation of kynurenine pathway dioxygenases by acute stress of mice

Glial and tissue-specific regulation of Kynurenine Pathway dioxygenases by acuteTo appear in: Neurobiology of Stress Received Date: 7 December 2016 Revised Date: 10 January 2017 Accepted

Glial and tissue-specific regulation of Kynurenine Pathway dioxygenases by acute

To appear in: Neurobiology of Stress

Received Date: 7 December 2016

Revised Date: 10 January 2017

Accepted Date: 7 February 2017

Please cite this article as: Dostal CR, Sulzer MC, Kelley KW, Freund GG, McCusker RH, Glial and

tissue-specific regulation of Kynurenine Pathway dioxygenases by acute stress of mice, Neurobiology of

Stress (2017), doi: 10.1016/j.ynstr.2017.02.002.

This is a PDF file of an unedited manuscript that has been accepted for publication As a service to our customers we are providing this early version of the manuscript The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Table 1 Assay specifics for analysis of murine Ido1, Ido2 and Tdo2 steady-state gene expression.

Probe 6-FAM CAGATTTCT Zen AGCCACAAGGACCCAGG IABkFQ

Reverse GCAGCTTTTCAACTTCTTCTCG

Probe 6-FAM TCGGGCAGC Zen TCCACATTACAATTCA IABkFQ

Probe 6-FAM ATTGAGAAC Zen GGGCAGCTTCGAGAA IABkFQ

Reverse CGCAGTAGGGAACAGCAATA

Probe 6-FAM CCAGAGGAT Zen TTGGAAGGAGAAAGCCAT IABkFQ

Reverse CGATTAAGTGAGGAAGTCTGAGG

Probe 6-FAM CCTCAGCTT Zen CTCGAACCCTGTAACTGTA IABkFQ

Reverse CTGCTCACGGTAACTCTTTAGG

Ido2-X2-mouse Set 1 Probe 6-FAM TGAAGAGAT Zen GAGCAATGAGCCGGT IABkFQ

Ido2-v2-ex3-4 set2 Reverse GAGGCATCTGTCCTGCCT

Probe 6-FAM CTGACCTGG Zen TGCTGACAAACTGGA IABkFQ

Reverse ACTGATTTCCAACGGTCCTTCT

Probe 6-FAM TAAAGAGTT Zen ACCGTGAGCAGCGCC IABkFQ

Reverse AAAGGTGCTGCCAAGATCTC

Probe 6-FAM TGAAGAGAT Zen GAGCAATGAGCCGGT IABkFQ

Reverse CCAAGTTCCTGGATACCTCAAC

Probe 6-FAM AGGACAGAT Zen GCCTCTCCTGGACT IABkFQ

Reverse ACGGTAACTCTTTAGGAATCTGC

Probe 6-FAM AAGAGGGTG Zen CTGCCAAGATCTCTT IABkFQ

Reverse CCAAGTTCCTGGATACCTCAAC

Probe 6-FAM ACCGCACAA Zen GTACAACCACACAGA IABkFQ

Reverse CTGGCGGTTCTCGATTAAGT

Tdo2-FL 1-2 Mm.PT.42.9084201.g Forward CCTGAGACACTTCAGTACTATGAG 99/99

Probe 6-FAM CCCGTTTGC Zen AGGAAACAGTGTAGGA IABkFQ

Tdo2-v2 0a-2 Tdo2 variant2 exons 0a 1.2 Forward GAAATGAGATCCGGGCTAAGAG 78

Probe 6-FAM TGGGTGCTG Zen ATTGGCTGTGTCT IABkFQ

Reverse GTGTATCTTTTATGTATCCTGATTGCC Ido2-v5 and Ido2-v6 sequence lie within Ido2/v2 (thus it is not possible to design an assay specific to Ido2-v5 or Ido2-

• Acute stress increased tryptophan metabolism to kynurenine

• Stress increased Ido1, Ido2 and Tdo2 expression by astrocytes

• Stress did not increase Ido2 and Tdo2 in astrocytes from Ido1KO mice

• Brain and hepatic Ido1, Ido2 and Tdo2 are differentially regulated by stress