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In this paper, we describe the use of a non-contact, radio-fre-quency detector to externally interrogate the conductance of polyaniline films prepared by inkjet and screen printing polyan

Trang 1

Non-contact, radio frequency detection of ammonia

with a printed polyaniline sensor

N.B Clarka,*, L.J Maherb

a

CSIRO Materials Science and Engineering, Private Bag 10, Clayton South 3169, Victoria, Australia

b Detection Systems Pty Ltd., P.O Box 397, Bayswater 3153, Victoria, Australia

a r t i c l e i n f o

Article history:

Received 9 February 2009

Received in revised form 17 March 2009

Accepted 29 March 2009

Available online xxxx

Keywords:

Sensor

Remote interrogation

Radio frequency

Polyaniline

Inkjet printing

Screen printing

a b s t r a c t

A novel system for the detection of ammonia was developed by monitoring the conductance of inkjet printed or screen printed polyaniline films with a radio frequency detector The system has the advantage

of non-contact detection of ammonia within sealed packages Since the sensor is a passive printed film that is externally interrogated, it does not require an internal power source or associated circuitry, and therefore may be a low-cost device suitable for smart packaging applications When printed on a suitable substrate, the sensor can be cycled several times using heat or a volatile acid to regenerate the polyaniline surface

Crown Copyright Ó 2009 Published by Elsevier Ltd All rights reserved

1 Introduction

A range of devices are available for the detection of ammonia

but most are costly and complex precision instruments

Accord-ingly, there has been continuing interest in simpler, lower-cost

designs that could be deployed more widely, such as those based

on thin films of polyaniline However, even these simple

polyani-line ammonia sensors generally require internal electrical power

and associated circuitry to operate as designed

Polyaniline (PAn) is a polymer that changes conductivity with

change in pH as a result of changes in the degree of protonation

of the polymer backbone, making it useful as a sensor for volatile

bases such as ammonia Unfortunately, polyaniline in the

conduct-ing state is difficult to process because it cannot be dissolved in

common solvents or melted below the decomposition

tempera-ture A common approach to overcoming this difficulty is to

prepare polyaniline in the form of dispersions suited to

conven-tional application methods

One method of preparing aqueous dispersions with high

stabil-ity is based on polyaniline nanofibres, as extensively reported by

Kaner’s group[1–17] Information and insights have accumulated

over several years but broadly, aqueous dispersions of polyaniline

can be formed by manipulating the reaction conditions to favour

homogenous nucleation, either by interfacial polymerisation, or more recently, by rapid mixing during the polymerisation reaction induction period, then allowing the reaction to proceed to comple-tion undisturbed Both methods result in the formacomple-tion of polyan-iline nanofibres with diameters in the 30–50 nm range, depending upon the acid used in the reaction to protonate the polymer and make it hydrophilic The polyaniline nanofibres are small enough

to be stabilised by the positive charge they carry in aqueous disper-sion Such stable aqueous dispersions can be used in inkjet ink, but concentrations are often lower than desirable for printing, as several ink layers may be required before useful conductivity is obtained

An alternative approach is the preparation of dispersions with a bulky hydrocarbon component to increase solubility and plasticity; for example, using dodecylbenzenesulfonic acid (DBSA) as a dop-ant as reported by Killard’s group[18–23] The DBSA acts as both

an anionic dopant and as a surfactant that stabilises the polyaniline dispersion against agglomeration Because of the improved stabil-ity of the polyaniline dispersion, PAn loading concentrations can be higher, which is better for printing

In this paper, we describe the use of a non-contact, radio-fre-quency detector to externally interrogate the conductance of polyaniline films prepared by inkjet and screen printing polyani-line-nanofibre and polyaniline-DBSA dispersions Since the sensor

is externally interrogated, it does not require an internal power source or associated circuitry, allowing the detection of ammonia inside sealed packages

1381-5148/$ - see front matter Crown Copyright Ó 2009 Published by Elsevier Ltd All rights reserved.

doi:10.1016/j.reactfunctpolym.2009.03.011

* Corresponding author Tel.: +61 395452259; fax: +61 395452448.

E-mail address: noel.clark@csiro.au (N.B Clark).

Contents lists available atScienceDirect Reactive & Functional Polymers

j o u r n a l h o m e p a g e : w w w e l s e v i e r c o m / l o c a t e / r e a c t

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2 Experimental

2.1 PAn dispersion preparation

DBSA (90%, technical grade, Fluka, Germany), ammonium

per-oxydisulfate (Analar, BDH, UK), Teric BL8 surfactant (C12

ethoxy-lated fatty acid alcohol, Huntsman, Australia) and sodium

dodecylsulfate (Analar, BDH, UK) were used as received

Hydro-chloric acid (37%, AR-grade, Merck, Germany) was diluted to

1 M with deionised water Aniline (AR-grade, Univar, Australia)

was distilled under vacuum with vigorous stirring to prevent

bumping

A PAn-nanofibre dispersion was prepared using the methods

described by Huang and Kaner[11] The purified aniline (3.2 mmol

or 0.3 g) was mixed with 10 mL of 1.0 M HCl acid solution

Ammo-nium peroxydisulfate (0.8 mmol or 0.18 g) was mixed into another

10 mL aliquot of the acid solution The aniline-acid solution was

added to the oxidant and the two solutions rapidly mixed for

30 s and then allowed to react undisturbed overnight The

follow-ing day, the polyaniline was washed with water and centrifuged

After three washings, the supernatant liquor had a pH of 3.2 and

was strongly green in colour, indicating the presence of polyaniline

particles too small to settle Before use in printing trials, any

remaining particles larger than 1lm were removed by passing

the dispersion through a 55-mm glassfibre filter (Whatman GFA,

UK) with vacuum assistance Teric BL8 was added to adjust surface

tension to a level within the operating envelope of the printer

PAn-DBSA dispersions were prepared, based on the method

reported by Ngamna et al.[21], but with several minor variations

First, the amount of DBSA used was half of that found by Ngamna

et al to be optimum for maximum PAn concentration (i.e 0.095 M

DBSA, rather than 0.19 M; because of the other modifications

em-ployed, the stability of the dispersion at this DBSA addition level

was acceptable) Second, the reaction was conducted with mixing

only during the reaction induction period and none thereafter, as

Kaner’s group had shown that continuous mixing during PAn

syn-thesis may increase particle agglomeration Third, ultrasonication

was used to disperse the PAn formed prior to filtration, to break

up any large particles and maximise dispersion concentration

and stability The procedure used was as follows:

DBSA (1.24 g) was added to 20 mL H2O, 0.60 g An, 20 mL 1 M

HCl and 0.73 g (NH4)2S2O8, and mixed for 30 s, and then reacted

undisturbed for 2.5 h The dispersion formed was dialysed against

600 mL of 0.05 M sodium dodecylsulfate through a cellulose

mem-brane (Sigma–Aldrich D9402) for 48 h with two solution changes,

then ultrasonicated with a 750 W Sonics Vibracell 1-cm diameter

titanium probe (treatment cycle was 1 s on, 2 s off, 70% of

maxi-mum amplitude, 10 min treatment time, equivalent to 3.3 min

to-tal ultrasonication time) and passed through a 55-mm glassfibre

filter (GFA, Whatman, UK) with vacuum assistance

The viscosity of the dispersions was measured with a Brookfield

DV II+ viscometer and surface tension was measured with the

rod-pull method[24,25] The contact angle of water drops applied to

each of the substrates was measured with a KSV Instruments Ltd

Cam 200 The UV–Vis spectra of the dispersions were collected

with a Perkin–Elmer Lambda 35 spectrophotometer, after dilution

to comparable absorbance PAn film thickness was measured on

PVC substrates by taking microtome cross sections and imaging

them with a Philips XL 30 Field Emission Scanning Electron

Microscope

The two PAn dispersions were cast directly on selected

sub-strates, or injected into the cartridge of a Dimatix Materials Printer

(model 2811 FUJIFILM Dimatix, USA) for inkjet printing trials An

aliquot of the PAn–DBSA dispersion was also evaporated to a paste

for application with a screen printing kit (Riso PG-5, Japan)

2.2 Substrates For the application trials, a range of substrates were employed

of commercial and general laboratory origin, the majority being polymer label stock that may be candidates for future commercial sensor deployment Based on Raman spectra (data not given for commercial reasons) the label stock samples were composed of polyethylene with TiO2 filler, with an ink receptor layer applied

to the surface Substrate pH was measured with a Hanna Instru-ments model 211 pH meter, on solutions prepared by chopping 0.5 g of each substrate into 2 mm strips and soaking in 10 g of deionised water for 72 h

2.3 Radio frequency conductance measurements

A PCIS-3000 10-95 6536 radio frequency detector (Detection Systems, Melbourne, Australia)[26]was used to monitor the con-ductance of sensor strips coated with the polyaniline dispersions PCIS equipment is typically used to detect missing and damaged items inside sealed packs as they are conveyed on high speed pro-duction lines This electromagnetic inspection technique is based

on a low voltage, low frequency AC electric field Sealed packages are conveyed through the PCIS transducer by means of a motorised conveyor and a series of ‘slices’ are recorded and assembled into an overall picture of the internal contents of the package A computer analyses this overall picture for any defects In traditional PCIS applications it is desirable to focus on the contents and ignore the RF signal generated by the packaging However, for the exper-iment described here, it was desirable to detect the signal from a sensor embedded in, or on, the packaging Further, the sensor con-ductivity was modulated by the local environment (i.e the concen-tration of ammonia inside the box) To our knowledge this is the first time such a device has been used as a component of a RF sen-sor system

2.4 Lumped circuit approximation

An equivalent circuit of the sensor while centred in the scan-ning head can be described as follows (refer toFig 1) The trans-mitted signal reaches the receiving electrode by two parallel paths – (1) directly capacitively coupled, and (2) coupled via the sensor Here, Cbypassis the direct coupling from the transmitting

Fig 1 Simple lumped circuit approximation of sensor in the PCIS transducer.

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to receiving electrode across the scanning head (substantial

rela-tive to the sensor path) C1is the coupling from the transmitting

electrode to the sensor strip Zsensoris the impedance of the PAn

sensor strip The sensor strip is mostly resistive in its conducting

state C2is the coupling from the top of the sensor strip to the

receiving electrode This model is useful for understanding the

ba-sics of the scanning head Some limitations of this model include:

 Impedances involved are very high, circuit elements are

distrib-uted and stray capacitances are present

 Packaging materials are neglected The impedances of materials

generally thought of as insulating (paper, cardboard, plastic, or

the conveyor belt) can be significant in value, but in practice

their effect can generally be mitigated Their response is

gener-ally constant, localised, or at a lower frequency than that of the

sensor or product

For the polyaniline sensor tests, a hinged polypropylene box of

dimensions 195 mm  55 mm  45 mm was employed, with the

sensor strip taped to the inside, vertically near the centre of the

longitudinal axis of the box (Fig 2) The composition of the plastic

was not important as the only requirement was for a constant

cross section Three drops of the test liquid, either ammonia

solu-tion or acetic acid, were applied by pipette to the inside of the box

along the front edge When closed, the box would quickly fill with

vapour from the applied liquid, potentially inducing a change in

the degree of protonation of the PAn chains on the sensor surface

Passing the box over the transmitter on the conveyor belt

al-lows the conductance of the PAn strip to be monitored, which in

turn is an indicator of the atmosphere within the box The

trans-mitter generates a low sinusoidal voltage, typically 8–20 V rms,

in the low frequency range The conveyor was 3-mm thick and

was driven by a variable speed drive, but with the same speed used

for all experiments The receiving assembly on the PCIS equipment

actually has two ‘lanes’ or inspection zones Because only one zone

was required for these tests the two zones were electrically

con-nected together, effectively forming one double sized inspection

area The amplifier converted the received signal to a low

imped-ance suitable for subsequent signal processing The amplified

sig-nal was converted to a DC voltage of relatively low frequency

These waveforms are the ‘RF signal vs time’ plots presented in this

paper Positive RF signals resulted if a conductive strip or sensor

was placed in the scanning head The strip or sensor acts as a

‘par-tial reduced impedance path’, compared with the air/bypass path The computer converted and stored the waveforms to disk Each waveform consisted of 500 samples over a duration of 2 s (250 Hz sampling rate) The range of the ‘RF signal’ is 0–4095, with

a baseline (nothing in the scanning head) around 2270 The stored waveforms were analysed using a spreadsheet Of particular inter-est was the height of the peak caused by the PAn sensor in the mid-dle of the package The time series of the height of this peak over time was also of key interest

3 Results and discussion 3.1 Substrate properties Important properties of the substrates used to support polyan-iline sensor films were the contact angle of applied water drops and pH (Table 1) Contact angle predicts wettability, which in turn may affect the uniformity of the applied polyaniline film, whereas substrate pH may affect the performance of the polyaniline film as

an ammonia sensor Accordingly, the best performance would be expected from substrates that were both hydrophilic (contact an-gles less than 90°) and acidic (pH of <7) Only the glass and filter paper met both these criteria

3.2 PAn dispersion properties The properties of the prepared PAn dispersions are listed in Ta-ble 2 The PAn-nanofibre dispersion had a total solids concentra-tion less than 1%, and not surprisingly, had properties not dissimilar to water This meant that surface tension in particular was higher than desirable for the Dimatix printer and two drops

of Teric BL8 surfactant (C12 ethoxylated fatty acid alcohol, Hunts-man) was added to reduce surface tension to 37 dyn cm 1prior to printing The PAn-DBSA dispersion had a solids concentration al-most two and a half times higher than that of the nanofibre disper-sion, and surface tension was within the range of the Dimatix printer The UV–Vis spectra were consistent with those expected for PAn-nanofibre [10] and PAn-DBSA dispersions, respectively

[21] 3.3 PAn-nanofibre application Test prints were made with the PAn-nanofibre dispersion on filter paper Because the PAn concentration was low, print intensity

Fig 2 Test arrangement using PCTS-3000 radio frequency detector.

Table 1 Substrates tested.

Label stock B KW Doggett, Australia 101 7.6

Label stock D Avery Dennison, Australia 74 8.1

Table 2 Dispersion properties relative to dimatix printer requirements.

Liquid Total solids (%) Viscosity (mPa S) Surface tension (dyn/cm)

Trang 4

remained poor even after several layers were applied in succession

(examples are therefore not reproduced here) When the

PAn-nanofibre dispersion was cast (rather than printed) on glass, a thin,

uniform film was formed that was soft and easily damaged by

handling

3.4 PAn-nanofibre radio frequency response

The PAn-coated glass slide was installed in the test box and

passed beneath the radio frequency head several times, with

ammonia or acetic acid solutions added successively to change

the box atmosphere as described above The radio frequency

wave-form was collected at each pass Output traces representing acidic

and alkaline atmospheres are presented in Fig 3 Under acidic

conditions, a large peak was evident in the output from the radio

frequency detector, corresponding to the position of the

PAn-nanofibre sensor strip in the box axial dimension Since the

position of the peak relative to the horizontal axis of the chart

reflected the physical position of the PAn strip inside the plastic box, moving the sensor strip within the box would result in a cor-responding lateral movement of the peak in the output trace The PAn peak amplitude varied according to the atmosphere within the box Peak maxima were obtained either initially, after the sensor strip was first installed with the PAn-nanofibre in the emeraldine salt form, or after exposure to acetic acid following pre-vious exposure to ammonia Peak minima (i.e baseline levels) were reached after exposure of the sensor strip to ammonia, caused by de-protonation of the amine groups in the PAn polymer

to the emeraldine base form Overall, the results demonstrated that the PAn-nanofibre sensor worked as reported by Kaner’s group[2], although the low concentration of the PAn-nanofibre dispersion meant that satisfactory prints would require many passes of the print head

Since the detector was sensitive enough to register disturbances

in the radio frequency field caused by transitions from air to plastic

as the box was conveyed through the detector, two peaks were

Box axis (mm)

2250 2300 2350 2400 2450 2500 2550 2600 2650

Acid Alkali

Fig 3 Radio frequency signal obtained from PAn-nanofibre dispersion cast on glass, with successive exposure to ammonia and acetic acid.

2360 2380 2400 2420 2440 2460 2480

Surface resistivity (KOhms/sq)

Fig 4 Relationship between radio frequency signal and surface resistivity measured with a 4-point probe.

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evident in the device output corresponding to the two ends of the

box, and three smaller bumps on the baseline (at 60 mm, 75 and

90 mm in the axial dimension) associated with shallow decorative

mouldings incorporated by the manufacturer in the box design

3.5 PAn-DBSA application

The image intensity obtained by inkjet printing the PAn-DBSA

dispersion on filter paper was satisfactory However, sharpness

suffered as a result of lateral migration of the dispersion along

the fibres and coverage was not completely uniform, with mottle

and print head scan lines both evident To determine the

relation-ship between RF signal and electrical resistance, filter paper was

inkjet printed with varying layers of the PAn-DBSA The surface

resistivities of the prints were measured with a 4-point probe

and related to the corresponding RF signal (Fig 4) It was evident

that the relationship was linear over much of the surface resistivity

range, although at low resistivity the RF Signal appeared to plateau

To achieve a fair comparison, the PAn-DBSA was cast on glass for the initial tests with the radio frequency detector In this case, the dispersion shrank dramatically on drying, leaving only a small area of the glass covered by the polymer, which appeared to be a film much thicker than that achieved using the PAn-nanofibre dis-persion Nevertheless, successive exposure to ammonia and acetic acid produced a series of peaks in the radio frequency detector out-put similar to those obtained with the PAn-nanofibre dispersion (Fig 5)

By abstracting the signal maxima of the sensor, it was possible

to plot signal strength against time (Fig 6) The sensor could be cycled by successive exposure to ammonia and acetic acid but on the third such cycle the baseline showed evidence of a rise in con-ductance, probably from accumulation of ammonium acetate Repeating this process for PAn-DBSA cast on each of the sub-strates listed in Table 1, several observations were made First, the results were primarily influenced by pH, with those substrates with alkaline surfaces (label stocks A, B, D and E) giving poor

Box axis (mm)

2250 2300 2350 2400 2450 2500 2550 2600 2650

Initial signal After ammonia After acetic acid

Fig 5 Radio frequency signal obtained from PAn-DBSA dispersion cast on glass, with successive exposure to ammonia and acetic acid.

2250 2300 2350 2400 2450 2500 2550 2600 2650

Time (min)

Fig 6 Radio frequency maxima obtained from PAn-DBSA dispersion cast on glass, with successive exposure to ammonia (squares) and acetic acid (circles).

Trang 6

results because of partial de-protonation of the PAn-DBSA film

be-fore exposure to ammonia Second, shrinkage of the PAn-DBSA film

on drying was a significant issue for smooth substrates like glass

and PVC, and also for the non-absorbent label stocks Overall, the

best results were obtained using the filter paper substrate, which

was low in pH and had a micro-texture that prevented PAn film

shrinkage on drying

Another method of cycling the sensor may be by simply heating the printed substrate in air, driving off the volatile ammonia as re-cently detailed by Crowley et al.[23] This approach was adopted for PAn-DBSA inkjet printed on filter paper (Fig 7) Although a sta-ble baseline was obtained because ammonium acetate could not accumulate, the sensor did not recover quickly on heating The DBSA dispersion, while more concentrated than the PAn-nanofibre dispersion, was still quite low in viscosity, as required for inkjet printing A consequence of this low viscosity was deep penetration of the dispersion in the z-direction, almost to the underside of the paper sheet Such deep penetration could slow the evaporation of ammonia, as the diffusion path through the coated paper matrix would be tortuous To test this concept, the dispersion was thickened by evaporation, forming a paste that could be applied by screen printing The thickness of a screen-printed film on PVC was measured from SEM images of cross

2250 2300 2350 2400 2450 2500 2550 2600 2650

Box axis (mm)

Fig 7 Radio frequency maxima obtained from PAn-DBSA dispersion, inkjet printed on filter paper, with exposure to ammonia and recovery by heating in air on a hotplate at

70 °C Ammonia additions (square points) result in steep declines in the RF signal, followed by gradual recovery with heating.

Table 3

Film thickness of PAn dispersions applied to PVC.

2250 2300 2350 2400 2450 2500 2550 2600 2650

Time (min)

Fig 8 Radio frequency maxima obtained from PAn-DBSA dispersion, screen printed on filter paper, with repeated application of ammonia (square points) and recovery by heating in air on a hotplate at 70 °C.

Trang 7

sections and compared with inkjet printed DBSA and

PAn-nanofibre (Table 3) Screen printing the PAn-DBSA dispersion onto

filter paper gave a rough and thick film compared to other methods

of application However, it gave an ammonia sensor that could be

rapidly and repeatedly cycled by heating in air on a hotplate at

70 °C (Fig 8)

4 Conclusions

Ammonia sensors were prepared by printing polyaniline

disper-sions on various substrates and using a radio frequency detector to

monitor conductance Dispersions of polyaniline doped with

dode-cylbenzenesulfonic acid could be prepared at higher concentration

than those of polyaniline nanofibres and consequently the former

were more suitable for inkjet printing on a range of substrates

However, these substrates varied in their suitability as sensor

sup-ports because of variations in both pH and contact angle, with the

best substrate found to be a filter paper with a low pH and a

micro-texture that obviated PAn-DBSA film shrinkage on drying The

ink-jet printed PAn-DBSA dispersion worked as an ammonia sensor but

cycling time on exposure to volatile acid or heat was slow,

proba-bly because of deep penetration of the low viscosity PAn-DBSA

ink-jet formulation through the sheet A screen-print paste prepared by

evaporating the PAn-DBSA inkjet formulation to a higher viscosity

was more successful, rapidly and repeatedly cycling simply by

heating in air Together, the PAn-DBSA and the radio frequency

detector form a non-contact sensor for the detection of ammonia

within sealed packages, and is therefore a low-cost device that

might be suitable for smart packaging applications

Acknowledgements

The authors are indebted to Dr Orawan Winther-Jensen (nee

Ngamna), of Monash University, for advice on the preparation of

PAn-DBSA dispersions The SEM images were collected by Mark

Greaves at CSIRO Materials Science and Engineering Contact angle

measurements were made with the assistance of Dr Christian

Kugge of CSME, using equipment located at CSIRO Molecular and

Health Technologies The authors are indebted to Dr Ken Wong

of Scion, New Zealand, Dr Nafty Vanderhoek and Dr Warwick

Rav-erty at CSIRO Materials Science and Engineering for valuable discussions and suggestions The work formed part of a New Zea-land Government FRST project entitled Functional Packaging Sys-tems for Food Exports and was financially supported by the Cooperative Research Centre for Functional Communication Sur-faces (CRC SmartPrint) and CSIRO Materials Science and Engineering

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