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Diagnosis of autoimmune pancreatitis with cholesterol granuloma mimicking intraductal papillary mucinous carcinoma: a case report

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Diagnosis of autoimmune pancreatitis with cholesterol granuloma mimicking intraductal papillary mucinous carcinoma A case report D m Y K a b a A R R A A K P P P D C 1 i r d c i d c c m S h 2 c CASE RE[.]

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CASE REPORT – OPEN ACCESS

International Journal of Surgery Case Reports 33 (2017) 62–66

jo u r n al ho me p a g e :w w w c a s e r e p o r t s c o m

Yusuke Takahashia,∗, Naoyuki Yokoyamaa, Daisuke Satoa, Tetsuya Otania,

Koko Mitsumab, Hideki Hashidateb

a Department of Digestive Surgery, Niigata City General Hospital, Niigata, 950-1197, Japan

b Department of Pathology, Niigata City General Hospital, Niigata, 950-1197, Japan

a r t i c l e i n f o

Article history:

Received 8 November 2016

Received in revised form 27 February 2017

Accepted 27 February 2017

Available online 1 March 2017

Keywords:

Pancreatic diseases

Pancreatic neoplasms

Pancreatic cyst

Ductal carcinoma of the pancreas

Case report

a b s t r a c t

INTRODUCTION:Pancreaticcystsareoftenobservedincidentallyonabdominalcomputedtomography (CT).Forcystsinvolvingintracysticnodules,malignantneoplasmssuchasintraductalpapillary-mucinous carcinoma(IPMC)shouldbesuspected.Incontrast,cholesterolgranuloma(CG)rarelyoccursinthe pan-creas,andCG-associatedautoimmunepancreatitis(AIP)hasnotyetbeenreported.Toourknowledge, thisisthefirstreportedcaseofAIPwithCGmimickingIPMC

PRESENTATIONOFCASE:A56-year-oldwomanunderwentabdominalCTforpreoperativebreastcancer screening.Asymptomaticpolycysticlesionsweredetectedinthepancreatictail(maximumdiameter,

5cm).Magneticresonancecholangiopancreatographyandendoscopicultrasonographyrevealedmain pancreaticductobstructionandalesionwithintracysticnodules(maximumdiameter,10mm).Serum levelsofpancreaticcancertumormarkersandIgG4werewithinnormalranges.BecauseIPMCwas sus-pected,distalpancreatectomyandsplenectomywithregionallymphadenectomywereperformedafter surgeryforbreastcancer.Pathologicalexaminationofthespecimenrevealednoepithelialneoplasm; however,cholesterolcrystals withforeignbodygiantcellswereobserved.Moreover,IgG4-positive plasmacells,diffuselymphocyteinfiltration,storiformfibrosis,andobliterativephlebitiswereidentified

inthenon-cysticpancreaticparenchyma.ThefinaldiagnosiswasAIPwithCG

DISCUSSION:CGinthepancreasisrareanditspathogenesisremainsunclear.Thefindingsofthepresent casesuggestthatchronicinflammationduetoAIPmaycauselocalbleeding,andthatareactiontothe leakedbloodcellscausesCG

CONCLUSIONS:Althoughpreoperativediagnosismaybedifficult,AIPwithCGshouldbeconsideredasa differentialdiagnosisinpancreaticcystsinvolvingnodularlesions

©2017TheAuthor(s).PublishedbyElsevierLtdonbehalfofIJSPublishingGroupLtd.Thisisanopen accessarticleundertheCCBY-NC-NDlicense(http://creativecommons.org/licenses/by-nc-nd/4.0/)

Asymptomaticcysticlesionsinthepancreasareoftendetected

incidentallyoncomputedtomography(CT),abdominal

ultrasonog-raphy,ormagneticresonanceimaging(MRI).Candidatesforthe

differentialdiagnosisof theselesionsgenerallyinclude

pseudo-cysts, true cysts, and various neoplasms Candidate neoplasms

includeintraductalpapillary-mucinousneoplasmssuchas

intra-ductalpapillary-mucinouscarcinoma(IPMC),serousormucinous

cystneoplasm,andsolidpseudopapillaryneoplasm[1–3].Froma

Abbreviations: AIP, autoimmune pancreatitis; CG, cholesterol granuloma; CT,

computed tomography; IgG4, immunoglobulin G4; IPMC, intraductal

papillary-mucinous carcinoma; MRI, magnetic resonance imaging.

∗ Corresponding author at: Niigata City General Hospital, Department of Digestive

Surgery, 7-463 Shumoku, Chuo-ku Niigata City, 950-1197, Japan.

E-mail address: niigata.takahashi@hosp.niigata.niigata.jp (Y Takahashi).

clinicalperspective,theidentificationofamalignantneoplasmis particularlyimportantinthesecasesbecauseoftheassociatedpoor prognosis

Cholesterolgranuloma(CG)isanodule-formingbenigndisease thatisoftenobservedinthemiddleearandpetrousapex,butrarely occursinthepancreas[4–6].CGoccursbecauseofaforeignbody reactiontocholesterolcrystals,whicharederivedfromthe degra-dationofbloodcomponents[4].ThepathogenesisofCGisthought

toberelatedtolocalchronicinflammation;however,the patho-physiologyofpancreaticCGhasnotbeenclarified[6,7].Inthiscase report,wepresentasurgicallytreatedcaseofautoimmune pan-creatitis(AIP)withCGthatmimickedIPMC.Thisworkhasbeen reportedinlinewiththeSCAREcriteria[8]

http://dx.doi.org/10.1016/j.ijscr.2017.02.053

2210-2612/© 2017 The Author(s) Published by Elsevier Ltd on behalf of IJS Publishing Group Ltd This is an open access article under the CC BY-NC-ND license ( http://

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Fig 1.Preoperative images a) Enhanced abdominal computed tomography showing polycystic lesions in the pancreatic tail (arrows) b) The cystic lesion in the pancreas appearing as a low-intensity area on T1-weighted magnetic resonance imaging (arrow) c) Magnetic resonance cholangiopancreatography showing obstruction of the main pancreatic duct in the proximity of the cystic lesions (arrow) d) Endoscopic ultrasonography showing several nodules (maximum diameter, 10 mm) in the cystic lesion (arrow) in the pancreas.

2 Presentation of case

Shehad nohistory of acutepancreatitis,abdominal trauma,or

otherrelated conditions.Thepancreaticcystsappearedas

low-and high-intensity areas onT1- and T2-weighted MRI,

respec-tively (Fig 1b) Magnetic resonance cholangiopancreatography

revealedobstructionofthemainpancreaticductinthe

proxim-ityofthecysticlesions(Fig.1c) Intracysticnodules(maximum

diameter,10mm)weredetectedusingendoscopic

ultrasonogra-phy(Fig.1d).Endoscopicretrogradepancreatographycouldnotbe

performedbecausecannulatingthepancreaticductwas

infeasi-ble.SerumlevelsofimmunoglobulinG4(IgG4)werewithinthe

normallimits,aswereserumlevelsofthetumormarkers

carci-noembryonicantigen,carbohydrateantigen/cancerantigen19-9,

DUPAN-2,and s-pancreas-1antigen.Onthebasisof these

find-ings,we suspectedIPMCand plannedsurgicaltreatment.Gross

examinationduringsurgeryrevealedahard-cystictumoratthe

pancreatictailthatcompressednearbyorgans.However,neither

localinvasion,nordistalmetastaseswerefound.Ondissectingthe

pancreas,milkywhitepancreaticjuicedrainedfromthemain

pan-creaticduct.Distalpancreatectomyandsplenectomywithregional

lymphadenectomywereperformed.Theoperativetimeandblood losswere183minand3010g,respectively

Onthesurface,theresectedpancreasshowednodularandcystic changes(Fig.2a).Thecutsectionrevealedcysticlesions contain-ingmilkyfluid,whichwasfoundtocontaincholesterol crystals whenexaminedmicroscopically(Fig.2b).Basedongross appear-ancealone,itwasdifficulttodeterminewhetherthelesionwas

a malignantneoplasm(such asanIPMCor mucinouscyst neo-plasm)orabenignlesion.Histologicalanalysisrevealedthatthe cysticlesionconsistedofadilatedmainpancreaticductand cavi-tiesofdegeneratedgranulomascontainingcholesterolcrystalsthat weresurroundedbyforeignbodygiantcells(Fig.2candd).In addi-tion,therestofthepancreatictissuearoundthelesionshowed prominentlymphocyteinfiltration,storiformfibrosis,obliterative phlebitis,andIgG4-positiveplasmacells,meetingthepathological diagnosticcriteriaofAIP,asrevisedbytheJapanPancreas Soci-etyin2011(Fig.3).Noneoplasticepitheliumwasobservedinthe mainorbranchedpancreaticducts.Onthebasisofthesefindings,

weultimatelydiagnosedthelesionasAIPwithCG.The postoper-ativecoursewasuneventfulandthepatientwasdischargedfrom ourhospital10daysafterthesurgery.Duringthe4-yearfollow-up periodaftersurgery,shehadremnantpancreatitistwice.Her rem-nantpancreatitiswassuspectedtobedrug-induced,ratherthan AIP.Itwasthoughttohaveresultedfromdrugs(eldecalcitoland/or letrozole)thatshewasprescribedaftersurgeryforbreastcancer,

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CASE REPORT – OPEN ACCESS

64 Y Takahashi et al / International Journal of Surgery Case Reports 33 (2017) 62–66

Fig 2. Resected specimen and findings of cholesterol granuloma a) It was impossible to distinguish the cholesterol granuloma from a malignant neoplasm based on macroscopic examination of the resected specimen b) The cut surface of the cystic lesions showed milky fluid c) Granulomatous and cystic lesions (arrows) surrounded the main pancreatic duct (*) (H&E, 5× magnification) d) Cholesterol crystals with inflammatory cells and foreign body giant cells were observed (H&E, 40× magnification) H&E: hematoxylin-eosin stain.

3 Discussion

[9].According toboth thediagnostic criteria for AIPthat were

proposedby theJapanese PancreaticSociety andthediagnostic

criteriafor type 1 AIPthat wereproposedby theInternational

Consensus Group,all characteristic pathological findings of AIP

wereconfirmedinthepresentcase[10–12].PatientswithAIP

usu-allyexhibitclinicalsignssuchaselevatedserumIgG4levelsand

extra-pancreaticlesions,apparentlybecauseAIPisanIgG4-related

disease.However,inourcase,noclinicalsignswereobservedother

thanthepathologicalfindingsintheresectedpancreas.Fromthis

perspective,ourcasewasquiteunusualincomparisonwith

typ-icalAIPcases.Inpractice,theunusualfeaturesofthecasemade

itdifficulttosuspect abenign lesionand ruleout the

neoplas-ticdifferentialdiagnosesbasedonpreoperativefindings.AIPisa benigndisease,andseldomrequiressurgerybecauseitresponds welltosteroids.Owingtoalackofpreviousliteratureregardingthis entity,detailpathophysiologyandmechanismofAIPwithCGare unknown;thiscasereportprovidesnewinsightsintotheseaspects Moreover,interestingly,inourpatient,thepostoperativeclinical courseisalsounusual,becauseshecurrentlyhasnoAIP-or IgG4-related symptomswithout steroidadministration aftersurgery, excludingdrug-inducedpancreatitis.Toelucidatepathophysiology andtheclinicalsignificanceofAIPwithCG,furthercasereports willbenecessary.Consequently,itisimportanttodistinguishthis diseasefrommalignantneoplasmsofthepancreas,suchasIPMC

Inourcase,CGmighthavebeencausedbyfocalbleedingdueto autoimmunepancreatitis.Moreover,pancreatitismightalsohave beenresponsiblefortheobstructionofthemainpancreaticduct thatwasdetectedonmagneticresonance cholangiopancreatogra-phy

High-intensityareasinbothT1-andT2-weightedimagesand otherdistinctiveMRIfeaturesarereportedlycharacteristicofCGin themiddleearandotherorgans,includingthepancreas[4,5].Inthe presentcase,thepancreaticcystslackedthesedistinctivefeatures Moreover,owingtotheabsenceofcharacteristicclinicalfeatures

ofAIP,suchasdiffuseorsegmentalpancreaticenlargementonCT andelevatedserumIgG4levels,apreoperativedefinitivediagnosis

ofAIPseemedtobeimpossibleinthiscase.Generally,ifpolycystic lesionsthatinvolvenodulesaredetectedinthepancreas,a

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malig-Fig 3. Pathological findings that met the diagnostic criteria of autoimmune pancreatitis a) Prominent lymphocyte and plasma cell infiltration (H&E, 40× magnification) b) Storiform fibrosis (H&E, 100× magnification) c) Obliterative phlebitis (arrow) (H&E, 40× magnification) d) Immunohistochemical staining for IgG4, showing >10 IgG4-positive plasma cells per high-power field (400× magnification) H&E: hematoxylin-eosin stain.

AIPwithCGisapotentiallybenigndisease.Therefore,toavoid

unnecessarysurgeries,thedifferentialdiagnosisofpancreatic

cys-ticlesionsshouldincludeAIPwithCGmimickingIPMC

None

Funding

Thisresearchdidnotreceiveanyspecificgrantfromfunding

agenciesinthepublic,commercial,ornot-for-profitsectors

Ethicalapprovalwasnotobtainedforthiscasereport

Consent

Writteninformedconsentwasobtainedfromthepatientfor publicationofthiscasereportandaccompanyingfigures.Acopyof thewrittenconsentisavailableforreviewbytheEditor-in-Chiefof thisjournalonrequest

YusukeTakahashi,NaoyukiYokoyama,DaisukeSato,Tetsuya Otani,KokoMitsuma,andHashidateHidekicontributedtothecase reportconception and design, and alsodraftedthemanuscript NaoyukiYokoyamaandTetsuyaOtaniparticipatedinthetreatment

ofthepatient.KokoMitsumaandHidekiHashidatediagnosedthis diseasepathologicallyandprovidedspecialcommentsabout speci-menfindings.NaoyukiYokoyamarevisedthemanuscriptcritically Allauthorsreadandapprovedthefinalmanuscript

This case report was not registered in a publicly accessible database

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CASE REPORT – OPEN ACCESS

66 Y Takahashi et al / International Journal of Surgery Case Reports 33 (2017) 62–66

Guarantor

Acknowledgment

None

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