Controlling swelling and release of hyaluronic acid during aqueous storage by in situ cross-linking during spray drying with alginate

Controlling swelling and release of hyaluronic acid during aqueous storage by in situ cross linking during spray drying with alginate 1 Submitted Article 1 2 3 Controlling swelling and release of hyal[.]

Hyaluronic acid or hyaluronan, is an important naturally occurring anionic

the-counter cosmetics has been limited by its high cost and poor storage stability in aqueous

(CLAMs) provide a storage method that is capable of incorporating cargo without chemical

hydrogen phosphate which was included at 0.25% (w/w) in solution All formulations were

Moisture Content (%) 5

Extent of (alginate) Cross- linking (%) 6

2.2.2 Monitoring Hyaluronic Acid Release in Water

aqueous solution (Strobel et al., 2017, Strobel et al., 2019b) To measure the extent of

The carbazole assay measures total uronic acids in solution (Bitter and Muir, 1962,

compared to its dry counterpart, is a measure of the product volume change due to absorption of

95% CI (p˂0.05) was done by One-Way ANOVA with Tukey’s pairwise comparisons when

The formation of CLAMs uses a calcium salt such as calcium hydrogen phosphate that is

Chelate Empty CLAMs, Figure 2b and c, respectively) had rougher surface topography

reflecting the tendency of the particles to aggregate in isopropanol (Figure 3) Despite efforts to

industry (Zhao, 2006, Liu and Rempel, 1997, Kiatkamjornwong, 2007, Bencherif et al., 2008)

Figure 4: a) and WAC (Equation 4) b) Plumping Ratio (Equation 5) of all cross-linked

3.4 Alginate and hyaluronic acid release during storage of microparticles in water

HA-CLAMs (Figure 5b) while 0% released from LV HA-CLAMs (Figure 5a) By 15 min, 64 ±

difference may be because hyaluronic acid could form calcium mediated cross-links within the

Chelate HA-CLAMs limited hyaluronic acid release to less than 22 ± 9 % within the 2-hour

release control with a mixed CLAMs matrix include improved toggling of storage and release for

Gao, C., Zhang, M., Ding, J., Pan, F., Jiang, Z., Li, Y & Zhao, J 2014 Pervaporation

Nath, S D., Abueva, C., Kim, B & Lee, B T 2015 Chitosan-hyaluronic acid polyelectrolyte

Zhao, X 2006 Synthesis and characterization of a novel hyaluronic acid hydrogel Journal of

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Biomaterials Science, Polymer Edition, 17, 419-433

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