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assessment of hypotension during dialysis as a manifestation of myocardial ischemia in patients with chronic renal failure

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8 aThe Critical Care Department, Cairo university, Egypt 9 Q3 bThe Critical Department in the National Liver Institute, Egypt 10 Received 4 September 2013; revised 3 May 2014; accepted 1

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8 aThe Critical Care Department, Cairo university, Egypt

9 Q3 bThe Critical Department in the National Liver Institute, Egypt

10 Received 4 September 2013; revised 3 May 2014; accepted 15 May 2014

11

14

15 Intradialytic hypotension

17 With chronic renal failure

19 Myocardial perfusion;

Abstract Introduction: Intradialytic hypotension (IDH) remains to be a major complication of hemodialysis occurring in nearly 25% of dialysis sessions It is a significant independent factor affecting mortality in hemodialysis patients Autonomic nervous system dysfunction, blood seques-tration in the setting of hypovolemia, cardiovascular diseases and increased plasma level of end products of nitric oxide metabolism are possible causes In this controlled prospective study we examined patients with chronic renal failure and intradialytic hypotension to evaluate the relation-ship between this hypotension and myocardial ischemia after controlling other possible causes Materials and methods: Thirty patients with chronic renal failure who are on regular dialysis were enrolled Before dialysis, patients were subjected to history taking and clinical examination Echocardiography and several lab tests were done Glomerular filtration rate (GFR) was calculated using Cockcroft’s and Gault formula Autonomic dysfunction was also assessed The dialysis ses-sion was standardized in all patients Intradialytic blood pressure was monitored and hypotenses-sion was classified as mild (SBP > 100 mmHg), moderate (SBP 80–100) or severe (SBP < 80) After dialysis, myocardial ischemia was assessed using stress myocardial perfusion imaging (MPI) (Phar-macologic stress testing using Dipyridamole) and is further classified as mild, moderate or severe ischemia Patients with sepsis, hemoglobin level less than 9 g/dL, diabetes mellitus, low cardiac out-put, coronary artery disease, significant valvular lesion or body weight below the dry weight of the patient were excluded from the study Bronchial asthma, emphysema and severe COPD are contra-indications to Dipyridamole and thus were also excluded from the study

Results: Twenty patients had no or mild intradialytic hypotension whereas ten patients had

* Corresponding author Address: 20 Abou Hazem st., Madkour,

Haram, Giza, Egypt Tel.: +20 1222402018.

E-mail address: randaalysoliman@hotmail.com (R.A Soliman).

Peer review under responsibility of The Egyptian College of Critical

Care Physicians.

Production and hosting by Elsevier

The Egyptian College of Critical Care Physicians The Egyptian Journal of Critical Care Medicine

http://ees.elsevier.com/ejccm

www.sciencedirect.com

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34 Intradialytic hypotension (IDH) remains to be a major

compli-35 cation of hemodialysis It occurs in nearly 25% of dialysis

ses-36 sions[1]and often requires aggressive resuscitative measures

37 and sometimes premature termination of hemodialysis It is

38 also a significant independent factor affecting mortality in

39 hemodialysis patients[2]

40 Despite the advances of machines with ultrafiltration

con-41 trol devices, modifying dialysate composition, temperature

42 control, correction of nutritional deficiencies and treatment

43 of anemia with erythropoietin therapy, many patients still have

44 episodes of intradialytic hypotension Among other factors,

45 the major pathophysiology of these episodes is the removal

46 of large volume of blood water and solutes over a short period

47 of time, overwhelming normal compensatory mechanisms,

48 which include plasma refilling and reduction of venous

capac-49 ity (due to reduction of pressure transmission to veins)

50 In some patients, a seemingly paradoxical and

inappropri-51 ate reduction of sympathetic tone may occur, causing

reduc-52 tion of arteriolar resistance, decreased transmission of

53 pressure to veins with corresponding increase in venous

capac-54 ity Increased sequestration of blood in veins under conditions

55 of hypovolemia reduces cardiac filling, cardiac output and

ulti-56 mately blood pressure Hypotensive episodes during

hemodial-57 ysis in patients with end stage renal disease in the absence of

58 inadequate maintenance of the plasma volume, pre-existence

59 of cardiovascular disease, or autonomic nervous system

dys-60 function are accompanied by increased plasma concentrations

61 of the end-products of nitric oxide metabolism (above the

62 expected levels, based on the reduction of urea[3])

63 In this controlled prospective study, patients with chronic

64 renal failure and intradialytic hypotension episodes were

thor-65 oughly investigated, to evaluate the relationship between

hypo-66 tension and myocardial ischemia after controlling other

67 possible causes

68 2 Patients and methods

69 This prospective study was conducted in King Fahd

Hemodi-70 alysis unit in Kasr Aini hospital, Hemodialysis Unit in

Al-71 Zahraa hospital and Critical Care Medicine Department in

72 Kasr Al-Aini hospital over the time period from February

73 2010 to June 2011 All patients included in the study provided

74 informed written consent The study includes 30 patients with

75 chronic renal failure who receive regular hemodialysis sessions

76 Twenty patients developed hypotension during hemodialysis

77 session, and the remaining 10 patients did not develop

hypo-Table 1 Baseline characteristics of the study group (n = 30)

Clinical

Duration of hemodialysis (years) 3.7 ± 2.1 ECG and ECHO

Grades of Albuminuria *

Urine specific gravity* 1009 ± 2.1 Labs

Serum Triglycerides (mg/dL) 109.7 ± 43 Serum Cholesterol (mg/dL) 151 ± 60

Serum Creatinine (mg/dL) 7.9 ± 2.1

Serum Potassium (mEq/L) 5.2 ± 0.63 IHD, ischemic heart disease; LVH, left ventricular hypertrophy; RWMA, regional wall motion abnormalities; LVEDD, left ven-tricle end-diastolic diameter; LVESD, left venven-tricle end-systolic diameter; EF, ejection fraction Hb, hemoglobin; Hct, hematocrit; TLC, total leukocytic count; FBS, fasting blood sugar; PPBS, post prandial blood sugar; HB A 1C , hemoglobin A 1C ; GFR, glomerular filtration rate.

* Done only for the 15 patients who were not anuric.

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78 tension during the sessions Myocardial ischemia was assessed

79 in all patients using stress myocardial perfusion imaging (MPI)

80 (Pharmacologic stress testing using Dipyridamole)

81 Patients with sepsis, hemoglobin level less than 9 g/dL,

82 diabetes mellitus, low cardiac output, acute coronary

83 syndrome, significant valvular lesion or body weight below

84 the dry weight of the patient were excluded from the study

85 Bronchial Asthma, emphysema and severe COPD are

86 contraindications to Dipyridamole and thus were also

87 excluded from the study

88 2.1 Before dialysis

89 All eligible patients were subjected to full history taking and

90 clinical examination Serum Urea and Creatinine were

mea-91 sured Glomerular filtration rate (GFR) was calculated using

92 Cockcroft’s and Gault equation Autonomic dysfunction was

93 assessed using at least 2 of the following tests; blood pressure

94 (BP) response to standing, BP response to sustained handgrip,

95 Heart Rate (HR) response to standing, HR response to deep

96 breathing and HR response to valsalva Positive result of

97 any test indicates autonomic dysfunction[4]

98 2.2 During dialysis

99 Dialysis was done via AV fistula (23 patients) or dialysis

cath-100 eter (7 patients), using Fresenius 4008B, Nipro machine with

101 ultrafiltration volume control and polysulfone, Fresenius F6

102 filters Temperature of dialysate was kept at 36C Blood

103 pump was kept between 250 and 350 ml/min except during

104 the hypotensive episodes during which the blood pump was

105 decreased to only 200 ml/min and not less to insure adequate

106 dialysis session Dialysate flow was 500 ml/min All dialysis

107 sessions lasted around 4 h

108 BP is recorded using standard sphygmomanometer every

109 30 min in supine position Each time the mean of 3

measure-110 ments is recorded Intradialytic hypotension is defined as a

111 symptomatic decrease of more than 30 mmHg in systolic blood

112 pressure or as an absolute systolic blood pressure under

113 90 mmHg[5] Hypotension is further classified as mild

(Sys-114 tolic Blood Pressure (SBP) > 100 mmHg), moderate (SBP

115 80–100 mmHg) and Severe (SBP < 80 mmHg)[6]

116

Patients who required vasopressors were unstable and

117

accordingly were excluded from this study

118

2.3 After dialysis

119

Within 2–5 h after dialysis, patients had both trans-thoracic

120

echocardiography and MPI The echo was done using an

121

ATL machine HDI 5000 with the patient lying in the left

lat-122

eral decubitus using a 3.5 MHZ probe MPI was done at the

123

nuclear laboratory of the critical care medicine department,

124

Kasr Al-Aini hospital, Cairo University utilizing the ‘‘freeze

125

imaging protocol’’ The set of SPECT images was acquired

126

using a triple head Siemens gamma camera with high

resolu-127

tion collimators (model Symbia E) Pharmacological stress

128

testing using Dipyridamole was done as most patients with

129

CKD could not achieve target HR during treadmill stress

test-130

ing due to marked physical limitations

131

Patients were instructed to fast for at least 6–8 h, stop

the-132

ophylline medications for at least 24 h and not to have any

caf-133

feinated drinks or beverages for at least 24 h prior to the study

134

Dipyridamole 0.56 mg/kg was diluted with 40 cc normal saline

135

and infused over 4 min 2 min later, 20–25 mCi Tc-99 m

Sestam-136

ibi were injected intravenously through a wide bore cannula

fol-137

lowed by saline flush Patients were monitored for at least 5 min

138

or till vital signs returned to baseline Ambulant patients were

139

asked to walk for 4 min after Dipyridamole infusion

140

Twenty projections were acquired (30 s for each frame) at

141

120 degree arc and total acquisition time of 12 min SPECT

142

images were processed using the back-projection technique to

143

get trans-axial images then short axis, vertical long axis and

144

horizontal long axis cuts The twenty-segment scoring system

145

was applied to estimate the Myocardium At Risk (MAR),

146

and the severity of perfusion defect was assessed for each

seg-147

ment using a ‘‘0–4’’ scoring system with ‘‘0’’ indicating normal

148

perfusion and ‘‘4’’ indicating no photon activity The sum of

149

these scores is the Summed Stress Score (SSS)

150

Seventy two hours later, patients were re-injected with 20–

151

30 mCi Tc-99 m Sestamibi intravenously to acquire the second

152

set of SPECT images at rest, and to estimate the left

ventricu-153

lar ejection fraction (EF) utilizing the Gated SPECT

tech-154

nique The severity of perfusion defects of MAR in this set

155

of SPECT images is assessed similarly and the sum of these

156

scores is the Summed Rest Score (SRS)

Figure 1 Myocardial ischemia diagnosed by MPI in patients with moderate or severe intradialytic hypotension, in comparison to those with no or mild hypotension (p = 0.002)

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157 The difference between SSS and SRS is the Summed

Differ-158 ence Score (SDS) It is classified as follows; 0–4 indicates no

159 ischemia, 5–8 mild ischemia, 9–12 moderate ischemia and

160 more than 12 is severe ischemia

161 2.4 Statistical methods

162 Statistical analysis was done using Statistical Package for

163 Social Sciences (SPSS) software, release 16.0.0 for Windows

164 (SPSS Inc., Chicago, Illinois)

165

Categorical variables are described as frequency (n) and

166

percentage (%) Quantitative variables are described as

167

mean ± standard deviation (SD) whenever parametric

Non-168

parametric quantitative variables are described as median

169

and interquartile range (IQR) Bivariate analysis of categorical

170

variables was done using Chi-square test with Yates

Continu-171

ity correction for 2· 2 tables Whenever cell frequency is <5,

172

Fisher’s Exact test is used

173

Comparison of two groups of quantitative variables was

174

done using Independent-Samples Student’s t test for

paramet-175

ric data, and Mann–Whitney test for non-parametric data

Labs

MPI

Data are displayed as n (%) or median (inter-quartile range).

IHD, ischemic heart disease; LVH, left ventricular hypertrophy; RWMA, regional wall motion abnormalities; LVEDD, left ventricle end-diastolic diameter; LVESD, left ventricle end-systolic diameter; EF, ejection fraction; Hb, hemoglobin; Hct, hematocrit; TLC, total leukocytic count; FBS, fasting blood sugar; PPBS, post prandial blood sugar; HB A 1C , hemoglobin A 1C ; GFR, glomerular filtration rate.

* Done only for the 15 patients who were not anuric.

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176 In all cases, the 2-sided significance was always taken as p

177 value p value <0.05 is considered statistically significant

178 3 Results

179 Thirty patients were included in this study Their baseline

180 characteristics are listed inTable 1

181 We found that 70% (7/10) of patients who had moderate or

182 severe hypotension (Group B) had myocardial ischemia on

183 MPI, in comparison to 10% (2/20) of patients who experienced

184 no or mild intradialytic hypotension (Group A); a difference

185 that is statistically significant (p = 0.002) (Fig 1)

186 Several other variables were compared across both study

187 groups (Table 2) It is to be noted that patients of Group B

188 were more likely to have stress induced LV dysfunction on

189 MPI (7/10, 70%) than patients of Group A (2/20, 10%)

190 (p = 0.002) (Fig 2) LVED was wider in Group A (Median

191

52 mm, IQR 46.5–55) than in Group B (44.5 mm, 44–50.75)

192

(p = 0.046) (Fig 3)

193

4 Discussion

194

Intradialytic hypotension (IDH) is a major complication of

195

hemodialysis Two to four liters of fluid needs to be removed

196

during a regular session, equivalent to 40–80% of the blood

197

volume It is therefore not surprising that hypotension occurs

198

so often Although many factors – patient or treatment related

199

– play a role, a reduction of blood volume is crucial in its

path-200

ogenesis[1]

201

Hypotension is one of the clinical presentations of CVD in

202

patients with CKD[7] There are a number of possible

expla-203

nations for the independent association of reduced GFR and

204

CVD outcomes First, a reduced GFR may be associated with

205

an increased level of nontraditional CVD risk factors that

fre-Figure 2 Stress induced LV dysfunction diagnosed by MPI in patients with moderate or severe intradialytic hypotension in comparison

to those with no or mild hypotension (p = 0.002)

Figure 3 Left ventricular end-diastolic diameter in patients with moderate or severe intradialytic hypotension (44.5 mm [44–51]) in comparison to those with no or mild intradialytic hypotension (52 mm [46.5–55]) (p = 0.046)

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220 efit as patients with preserved GFR Finally, decreased GFR

221 itself may be a risk factor for progression of ventricular

remod-222 eling and cardiac dysfunction[7]

223 Different studies stated that, during hemodialysis, patients

224 are particularly susceptible to myocardial ischemia for number

225 of reasons including: LV hypertrophy[8], intradialytic

hypo-226 tension and instability[9], high prevalence of decreased

coro-227 nary flow reserve even in the absence of coronary vessel

228 stenosis[10,11]

229 Hakeem et al.[12]reported that in patients with CKD 40%

230 of perfusion scans were abnormal (SSS P 4) with 20% mild

231 defects and 20% moderated to severe defects

232 Q4 In line with Hakeem’s result, we found that patients who

233 experience moderate or severe intradialytic hypotension have

234 significantly higher prevalence of myocardial ischemia (70%,

235 7/10), in comparison to those who have no or mild

intradialyt-236 ic hypotension (10%, 2/20) (p = 0.002)

237 Paoletti et al.[13]stated that there is growing evidence that

238 patients with CKD have unrecognized LV dysfunction, both

239 systolic and diastolic They also pointed at the importance of

240 LV dysfunction (LV ejection fraction <40%) as a predictor

241 of cardiac death in patients with CKD

242 We similarly found that CKD patients who develop

moder-243 ate or severe intradialytic hypotension, have significantly

244 higher prevalence of stress induced LV dysfunction (70%, 7/

245 10) than those who have no or mild hypotensive episodes

246 (10%, 2/20) (p = 0.002)

247 Deterioration of renal function in CKD may lead to

dysli-248 pidemia or accumulation of uremic toxins, which can stimulate

249 oxidative stress and inflammation, which in turn contributes to

250 endothelial dysfunction and progression of atherosclerosis

251 [14] Renal failure causes changes in plasma components and

252 endothelial structure and function that favor vascular injury,

253 which may play a role as a trigger for inflammatory response

254 Dyslipidemia associated with CKD contributes to the

inflam-255 matory response in renal failure[15] However, in our study

256 we found that levels of serum cholesterol and triglycerides were

257 not significantly different between the two study groups, a

258 finding probably attributed to the small number of the study

259 group and the relatively low economic standard of those

260 patients

261 Patients with CKD also have a high prevalence of

arterio-262 sclerosis and remodeling of large arteries Remodeling may

263 be due to either pressure overload – which is distinguished

264 by wall hypertrophy and an increased wall-to-lumen ratio –

265 or flow overload, which is characterized by a proportional

266 increase in arterial diameter and wall thickness[16]

279

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280

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282

Leveille M, et al Impact of nitric oxide on blood pressure in

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[4] Ewing DJ, Campbell IW, Murray A, Neilson JM, Clarke BF.

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Immediate heart-rate response to standing: simple test for

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