To evaluate the interaction properties of Rabaptin-5d with the small GTPases Rab4 and Rab5, we have applied protein interaction assays using the yeast two-hybrid system and a glutathione
Trang 1differ in their ability to interact with the small GTPase
Rab4
Elena Korobko1,2, Sergey Kiselev1, Sjur Olsnes3, Harald Stenmark3and Igor Korobko1
1 Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia
2 University of Oslo, Centre for Medical Studies at Moscow, Moscow, Russia
3 Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
Intracellular membrane transport is an important
pro-cess for eukaryotic cells Intracellular membranes are
organized in compartments, and transport between
compartments requires high specificity and tight
regu-lation The intercompartmental transport typically
occurs through transport vesicles budding from a
donor compartment and fusing to an acceptor
com-partment In this process, Rab GTPases were
demon-strated to play a central role by regulating vesicle
budding, motility and fusion [1] Another group of
molecules, v- and t-SNAREs, were suggested to
con-tribute to specificity of vesicle targeting [2–4] although
the specificity of the transport is also maintained by
Rab GTPases [5] A large number of Rab GTPases
have been identified in mammalian cells, and each of them seems to regulate a specific step in membrane transport through their downstream effectors, which are recruited by the GTPases in their active GTP-bound conformation [1]
The Rab5 GTPase is a regulator of early endocytic events in the cell [6] It plays a role in the formation of clathrin-coated vesicles at the plasma membrane [7], in their heterotypic fusion with early endosomes and homotypic fusion of early endosomes [8,9], and in microtubule-dependent motility of endocytic vesicles [10] By now, several Rab5 effector molecules have been identified that, in a coordinated way, act in a chain of molecular events (reviewed in [11]) One of
Keywords
endocytosis; Rab5; Rab4; Rabaptin-5
isoform; effector molecule
Correspondence
E Korobko, Institute of Gene Biology,
Russian Academy of Sciences, 34 ⁄ 5 Vavilov
Street, Moscow 119334, Russia
Fax: +7 095 1354105
Tel: +7 095 1359970
E-mail: alenavk@igb.ac.ru
(Received 19 July 2004, accepted 16 August
2004)
doi:10.1111/j.1432-1033.2004.04399.x
Rabaptin-5 is an effector for the small GTPase Rab5, a regulator of the early steps in endocytosis In addition, Rabaptin-5 interacts with the small GTPase Rab4 that has been implicated in recycling from early endosomes
to the cell surface Recently we have identified a ubiquitous transcript encoding the Rabaptin-5 isoform, Rabaptin-5d To evaluate the interaction properties of Rabaptin-5d with the small GTPases Rab4 and Rab5, we have applied protein interaction assays using the yeast two-hybrid system and a glutathione S-transferase pull-down assay We found that unlike Rabaptin-5, that interacts with both GTPases in GTP-bound conforma-tions, Rabaptin-5d interacts only with GTP-bound Rab5, and does not interact with Rab4, presumably due to a disrupted Rab4 binding site Immunofluorescence microscopy analysis carried out to address the locali-zation of Rabaptin-5d relative to GTP-bound Rab4 and Rab5 in BHK-21 cells supported these data Our data suggests that while Rabaptin-5 was proposed to act as a molecular linker between Rab5 and Rab4, to coordi-nate endocytic and recycling traffic, Rabaptin-5d is involved only in the Rab5-driven events
Abbreviations
3AT, 3-amino-(1,2,4)-triazole; EGFP, enhanced green fluorescent protein; GEF, guanine nucleotide exchange factor; GAL4AD, GAL4
transcriptional activating domain; GAL4BD, GAL4 DNA-binding domain; GST, glutathione S-transferase; SD, synthetic dextrose;
TGN, trans-Golgi network.
Trang 2and heterotypic fusion between early endosomes and
clatrin-coated vesicles [12–14] Cytosolic Rabaptin-5 is
complexed with Rabex-5, a Rab5 guanine nucleotide
exchange factor (GEF), and both proteins act
syner-gistically to activate Rab5 in early endosome fusion
events [13,15]
As well as being a Rab5 effector, Rabaptin-5 has
been suggested to play a role in connecting different
steps of the membrane transport process First,
Rabaptin-5 was demonstrated to interact through a
distinct binding domain with another small GTPase,
Rab4 [14], which was implicated in regulation of
rapid recycling from early endosomes [16,17] Second,
Rabaptin-5 can participate in balancing and
coordina-tion of endo- and exocytosis through interaccoordina-tion with
Rabphilin, a regulator of the exocytic pathway
[18,19] Finally, Rabaptin-5 interacts with the ear
domains of c1-adaptin, a subunit of the AP-1 adaptor
complex of clathrin-coated vesicles derived from
trans-Golgi network (TGN) [20], and GGAs, a family
of Arf-dependent clathrin adaptors involved in
selec-tion of TGN cargo [21] This reveals a funcselec-tional link
between proteins regulating TGN cargo export and
endosomal tethering⁄ fusion events through
Rabaptin-5 Thus, Rabaptin-5, along with several other
pro-teins, emerges to be a multivalent effector molecule
with a possible role in the regulation of
subcompart-mental organization and sorting of membrane vesicles
[22]
Rabaptin-5 is a 100-kDa protein encoded by the
RAB5EPgene Recent studies revealed that in addition
to the main transcript, a number of minor transcripts
exist that bear small deletions in the coding region
[23–25] Evidence was provided that these transcripts
are probably generated by alternative splicing from a
single pre-mRNA [23] One of the recently identified
Rabaptin-5 isoforms is Rabaptin-5d [23] Compared to
Rabaptin-5, this isoform has a short deletion of 40
amino acid in the N-terminus The deleted region is
partially inside of the second N-terminal coiled-coil
domain of Rabaptin-5 [14,23] (Fig 1) The ubiquitous
occurrence of the Rabaptin-5d transcript suggests that
this protein could play a significant role in the cell,
probably through modulation of the Rabaptin-5
func-tions In an attempt to clarify this suggestion, and to
better understand the functional role of Rabaptin-5d,
we have characterized this molecule with respect to
its ability to interact with the known Rabaptin-5
interaction partners, Rab4, Rab5 and Rabex-5 as well
as its ability to be recruited to specific endosomal
compartments
Results Rabaptin-5d interacts differentially with the small GTPases Rab4 and Rab5
The interaction of Rabaptin-5 with the small GTPases Rab4 and Rab5 can be assessed readily in the yeast two-hybrid system [12,14] We therefore used this assay
to analyze the interaction of Rabaptin-5d with Rab4 and Rab5
Similarly to Rabaptin-5, Rabaptin-5d was not able
to bind Rab4 or Rab5 in the inactive, GDP-bound form as no HIS3 reporter gene trans-activation was observed upon coexpression of GAL4AD–Rabaptin-5
or -Rabaptin-5d and GAL4BD fused to Rab5S34N or Rab4S22N, dominant negative mutants of Rab5 and Rab4 with decreased affinity for guanine nucleotides (Figs 2 and 3) Likewise, no HIS3 reporter gene trans-activation was revealed upon coexpression of the wild-type Rab5 bait and the Rabaptin-5d isoform prey (Fig 2), which is consistent with no detectable reporter gene activation upon Rab5 bait and full-length Rabap-tin-5 prey coexpression [14] Therefore, the GTPase-deficient mutant of Rab5, Rab5Q79L, was used as a bait to assay the interaction with Rabaptin-5d
Similarly to what has been reported for Rabaptin-5 [12,14], expression of the Rabaptin-5d prey together with the Rab5Q79L bait resulted in the HIS3 reporter gene trans-activation (Fig 2)
Unlike Rab5, both wild-type Rab4 and its GTPase-deficient mutant bait coexpression with full-length Rabaptin-5 prey have been shown to result in readily detectable reporter gene trans-activations [14] We
R4BD
R4BD
Rabaptin-5
5 135
187-226
R5BD
R5BD
aa
R4BD
140 295
R4BD
Fig 1 Schematic representation of Rabaptin-5 and its d isoform Coiled-coil regions (CC1–1, CC1–2, CC2–1, CC2–2) are shown in black Positions of the Rab4 binding domain (R4BD) are marked as white and black hatched boxes as determined in [14] and [26], respectively The Rab5 binding domain (R5BD) is marked as a dot-filled box The region deleted in Rabaptin-5d is shown in white Positions of coiled-coil regions CC1-1 and CC1-2, Rab4 binding domain, and the region deleted in Rabaptin-5d are numbered according to the amino acid sequence of mouse Rabaptin-5 (Gene-Bank Accession No D86066).
Trang 3observed a similar pattern of the reporter gene
trans-activations for Rabaptin-5 (Fig 3) However, when
Rabaptin-5d was assayed as a prey in the yeast
two-hybrid system, no HIS3 reporter gene trans-activation
was observed with neither bait, wild-type Rab4 nor its
GTPase-deficient mutant (Fig 3) suggesting the lack
of interaction between Rab4 and Rabaptin-5d
The interaction properties of the Rabaptin-5
iso-forms with Rab4 and Rab5 were further assayed in
glutathione S-transferase (GST) pull-down assays
GST–Rab4 preloaded with either GDP or the
unhy-drolyzable GTP analogue, GTPcS, was unable to pull
down enhanced green fluorescent protein
(EGFP)-tagged Rabaptin-5d from cytosol of transiently
trans-fected BHK-21 cells (Fig 4A) At the same time,
consistent with the data from yeast two-hybrid system,
EGFP–Rabaptin-5d was pulled down by
GTPcS-loa-ded but not with GDP-loaGTPcS-loa-ded GST-Rab5; similar to
Rabaptin-5 (Fig 4B) To exclude the possibility that
the EGFP-tag located at the N-terminus of
Rabaptin-5d, which is close to the mapped Rab4 binding site,
might interfere with Rab4 binding, similar experiments were performed with cytosols prepared from BHK-21 cells transiently expressing Rabaptin-5 or Rabaptin-5d with a C-terminal FLAG tag (DYKDDDDK) Simi-larly to experiments with EGFP-tagged proteins, GST–Rab4 was unable to pull down Rabaptin-5d– FLAG in either GDP or GTPcS-bound form At the same time, Rabaptin-5 was specifically pulled down from cytosol by GTPcS-loaded GST-Rab4 (Fig 4C)
In summary, both protein interaction analyses in the yeast two-hybrid system and GST pull-down assays suggest that Rabaptin-5d can specifically interact only with Rab5 but not with Rab4
Cytosolic Rabaptin-5d is complexed with Rabex-5 The Rab5 effector properties of Rabaptin-5 are mani-fested upon complexing of Rabaptin-5 with Rabex-5 [13,15] Although Rabaptin-5 can interact with Rab5–
Fig 2 Interaction specificity of Rabaptin-5d with Rab5 in the yeast
two-hybrid system Y153 reporter yeast cells were cotransformed
with plasmids encoding GAL4BD alone or fused to Rab5 or its
mutants, and GAL4AD alone or fused to Rabaptin-5 (Rn5) or
Rabaptin-5d (Rn5d) HIS3 reporter gene activation caused by
inter-action between GAL4BD- and GAL4AD-fused proteins in yeast was
assessed by spotting onto synthetic minimal medium
supplemen-ted (bottom) or not supplemensupplemen-ted (top) with 25 m M
3-amino-(1,2,4)-triazole (3AT).
Fig 3 Interaction specificity of Rabaptin-5d with Rab4 in the yeast two-hybrid system Y153 reporter yeast cells were cotransformed with plasmids encoding GAL4BD alone or fused to Rab4 or its mutants, and GAL4AD alone or fused to Rabaptin-5 (Rn5) or Rabap-tin-5d (Rn5d) HIS3 reporter gene activation caused by interaction between GAL4BD- and GAL4AD-fused proteins in yeast was assessed by spotting yeast onto synthetic minimal medium supple-mented (bottom) or not supplesupple-mented (top) with 25 m M 3AT.
Trang 4GTP in vitro, Rabaptin-5 alone could not support
bio-logical activity of Rab5 in early endosome fusion [12]
We therefore asked if the Rabaptin-5d isoform is
complexed with Rabex-5 in vivo As shown in Fig 5,
Rabex-5 coimmunoprecipitates with EGFP-tagged
ated with the Rab5 GEF, Rabex-5, in vivo
Subcellular localization of Rabaptin-5d upon coexpression with GTPase-deficient mutants of Rab4 or Rab5 GTPases
The findings based on the yeast two-hybrid system and GST pull-down analyses suggest that, whereas Rabap-tin-5 interacts with both Rab4 and Rab5 in their GTP-bound forms, Rabaptin-5d can interact only with GTP-bound Rab5 and not with GTP-bound Rab4 To address the question of how relevant these findings are
to the protein interaction properties in vivo, we next examined the subcellular localization of Rab4, Rab5 and Rabaptin-5d by confocal immunofluorescence microscopy As the available antibodies against Rab4 and Rab5 failed to detect the endogenous proteins, we coexpressed myc-tagged Rab4Q67L or Rab5Q79L with Rabaptin-5 isoforms as a C-terminal fusion partner of EGFP
Expression of the GTPase-deficient Rab5 mutant results in the appearance of enlarged Rab5-positive early endosomes that also recruit Rabaptin-5 [12] Consistent with the results of protein interaction assays
in vitro and in the yeast two-hybrid system, EGFP-Rabaptin-5d was localized on enlarged myc-positive vesicular structures when coexpressed with myc-Rab5Q79L in BHK-21 cells (Fig 6B,B¢) In this case, localization of EGFP and the myc-epitope in cotransfected cells were similar to those observed upon
B
C
Fig 4 Interaction specificity of Rabaptin-5d with Rab4 and Rab5 in
a GST pull-down assay GST–Rab4 (A, C) or GST-Rab5 (B) were
immobilized on Gluthatione Sepharose 4B beads and preloaded
with either GDP or GTPcS as indicated EGFP-tagged, or EGFP
alone (E) (A, B) or C-terminally FLAG-tagged Rabaptin-5 (5),
Ra-baptin-5d (5d) (C) were pulled down from cytosols prepared from
BHK-21 cells transiently expressing respective proteins Pulled
down proteins (upper panels) and aliquots of cytosols (bottom
pan-els) were separated in SDS ⁄ PAGE and immunoblotted with
poly-clonal antiserum against Rabaptin-5 (A, B) or with anti-FLAG-M2
monoclonal Igs (C) Arrows indicate endogenous Rabaptin-5 (Rn5)
and EGFP-fused Rabaptin-5 and its isoform (E-Rn5’s) (A,B), and
FLAG-tagged Rabaptin-5 and its isoform (Rn5-FLAG) (C).
D C
Fig 5 Cytosolic Rabaptin-5d expressed in BHK-21 cells is com-plexed with Rabex-5 Cytosols were prepared from BHK-21 cells transiently expressing EGFP alone (E) or EGFP C-terminally fused with Rabaptin-5 (5) or Rabaptin-5d (5d) Cytosols (A, C) or proteins immunoprecipitated from cytosols with anti-EGFP Igs (B, D) were separated by SDS ⁄ PAGE and immunoblotted with polyclonal anti-sera against Rabaptin-5 (A, B) or against Rabex-5 (C, D) Arrows indicate endogenous Rabaptin-5 (Rn5) and EGFP-fused Rabaptin-5 and its isoform (E-Rn5’s), Rabex-5 (Rabex-5), and antibody used for immunoprecipitation (Ab).
Trang 5coexpression of EGFP–Rabaptin-5 and myc-tagged
Rab5Q79L (Fig 6A,A¢)
When a myc-tagged GTPase-deficient mutant of
Rab4, Rab4Q67L, was coexpressed in BHK-21 cells
with EGFP–Rabaptin-5, we found that the two
pro-teins colocalize on vesicular structures in the
perinu-clear area (Fig 7A,A¢ and insets), which is consistent
with previous reports [14,24] Whereas
EGFP–Rabap-tin-5d also concentrated in the perinuclear region when
coexpressed with myc-tagged Rab4Q67L, the two
pro-teins did not show any significant colocalization
(Fig 7B,B¢ and insets)
To exclude the possibility that N-terminally fused
EGFP protein influences Rab4-binding properties of
Rabaptin-5d, similar colocalization experiments were
performed with N-terminally FLAG-tagged Rabap-tin-5 and RabapRabap-tin-5d Figure 8 shows that FLAG– Rabaptin-5d does not colocalization with Rab4Q67L-positive structures while FLAG–Rabaptin-5 inten-sively colocalizes with Rab4Q67L
Taken together, the data from confocal immunofluo-rescence microscopy provide further support that, unlike Rabaptin-5, Rabaptin-5d exclusively interacts with Rab5 but not with Rab4
Discussion
We have identified previously a cDNA encoding a novel protein, Rabaptin-5d This protein is similar to Rabaptin-5 but has small deletions in the N-terminal
Fig 6 Confocal immunofluorescence
analysis of BHK-21 cells coexpressing
myc-tagged Rab5Q79L and EGFP-myc-tagged
Rabap-tin-5 or EGFP-tagged RabapRabap-tin-5d Cells
cotransfected with myc-tagged Rab5Q79L
and EGFP-tagged Rabaptin-5 (A–A¢) or
Rabaptin-5d (B–B¢) were stained with mouse
monoclonal anti-(myc 9E10 Ig) followed by
Alexa546-conjugated anti-mouse secondary
Igs (A¢, B¢), or EGFP fluorescence was
recorded (A, B) Overlays are shown in
A and B with yellow color indicating
colocalization, red color indicating myc–
Rab5Q79L, and green color indicating
EGFP-fused proteins Panel dimensions are
80 lm 80 lm.
Fig 7 Confocal immunofluorescence
analysis of BHK-21 cells coexpressing
myc-tagged Rab4Q67L and EGFP-myc-tagged
Rabap-tin-5 or EGFP-tagged RabapRabap-tin-5d Cells
cotransfected with myc-tagged Rab4Q67L
and EGFP-tagged Rabaptin-5 (A–A¢) or
Rabaptin-5d (B–B¢) were stained with mouse
monoclonal anti-(myc 9E10 Ig) followed by
Alexa546-conjugated anti-mouse secondary
antibodies (A¢, B¢), or EGFP fluorescence
was recorded (A, B) Overlays are shown in
A and B with yellow color indicating
colo-calization, red color indicating
myc-Rab4Q67L, and green color indicating
EGFP-fused proteins The black-and white insets
in panels A¢ and B¢ shows higher
magnifica-tion of indicated regions for green (left) and
red (right) channels Panel dimensions are
100 lm 100 lm.
Trang 6portion of the polypeptide chain [23] Rabaptin-5 was
identified initially as a Rab5 effector protein that
specif-ically interacted with Rab5 in the GTP-bound form and
was an essential component for homotypic early
endo-some fusion and heterotypic fusion between
clathrin-coated vesicles and early endosomes [12–14] It was
pro-posed that Rabaptin-5 functioned by coupling Rab5
to its GEF, Rabex-5, which is bound to Rabaptin-5
[13,15] Besides a Rab5 effector function, the specific
interaction between Rabaptin-5 and another small
GTPase in the GTP-bound conformation, Rab4, was
demonstrated [11,14] thus suggesting the possibility that
Rabaptin-5 could function as a linker between two
sequential steps in membrane transport; early endosome
fusion and recycling [22] Here we report the
characteri-zation of Rabaptin-5d isoform interaction properties
with the small GTPases, Rab4 and Rab5
The analyses in the yeast two-hybrid system and
pull-down experiments suggest that Rabaptin-5d
spe-cifically interacts with Rab5 in its GTP-bound
confor-mation However unlike Rabaptin-5, Rabaptin-5d prey
failed to trans-activate reporter genes when either
Rab4 or its GTPase-deficient mutant were used as a
bait This suggests a lack of interaction between Rab4
and Rabaptin-5d, and this was further supported by
results of GST pull-down experiments Interestingly,
both the deletion found in the Rabaptin-5d
polypep-tide chain and the Rab4 binding site of Rabaptin-5 are
located in the N-terminal portion of protein However,
some discrepancy exists in the literature concerning the
position of the Rab4-binding site While Vitale et al
mapped it between amino acids 5 and 135, by
analyz-ing protein–protein interactions in yeast two-hybrid
system [14], the recent findings of Deneka et al suggest
that the Rab4-binding motif of Rabaptin-5 resides between amino acids 140 and 295 as demonstrated in GST pull-down experiments [26] (Fig 1) To clarify this point, we assayed the interaction properties of dif-ferent N-terminal fragments of Rabaptin-5 with Rab4
in the yeast two-hybrid system Consistent with the data of Deneka et al [26], amino acids 140–294 of Rabaptin-5 were sufficient to interact with Rab4 as judged by trans-activation of the LacZ reporter gene, while a polypeptide consisting of the first 149 amino acids was not (Fig 9) At the same time, a fragment of Rabaptin-5d corresponding to amino acids 140–294 of Rabaptin-5, which contains the deleted region, was unable to bind Rab4 In addition, the first 251 amino acids of the Rabaptin-5c isoform, bearing a natural deletion of amino acids 22–64 of Rabaptin-5 [23], was able to interact with Rab4, thus demonstrating the dis-pensability of these amino acids for the interaction (Fig 9) Taken together, the conclusion can be made that Rab4 binding site is located between amino acids
140 and 294 of Rabaptin-5 Rabaptin-5d has amino acids 187–226 deleted from the polypeptide chain, which is inside the minimal Rab4 binding fragment This suggests that the deletion disrupts the Rab4 bind-ing site of Rabaptin-5, which is further supported by the demonstrated lack of interaction between Rab4 and Rabaptin-5d
The colocalization studies in BHK-21 cells support the assumption that Rabaptin-5d can interact with Rab5 but not with Rab4 Accordingly, the recruitment
of EGFP–Rabaptin-5d on the enlarged Rab5Q79L-positive endosomes was observed whereas no apparent colocalization with Rab4Q67L was seen Finally, we observed association of Rabaptin-5d with Rabex-5
ysis of BHK-21 cells coexpressing myc-tagged Rab4Q67L and FLAG-myc-tagged Rabaptin-5 or FLAG-tagged Rabaptin-5d Cells cotransfected with myc-tagged Rab4Q67L and FLAG-tagged
Rabaptin-5 (A–A¢) or Rabaptin-Rabaptin-5d (B–B¢) were stained with mouse monoclonal anti-(myc 9E10 Ig) and anti-FLAG rabbit polyclonal Igs fol-lowed by Alexa546-conjugated anti-mouse and Alexa488-conjugated anti-rabbit secon-dary Igs Red (A¢, B¢) or green (A, B) fluores-cence was recorded Overlays are shown in
A and B with yellow color indicating colo-calization, red color indicating myc-Rab4Q67L, and green color indicating FLAG-tagged proteins Panel dimensions are 100 lm 100 lm.
Trang 7in vivo Taking into account that association of
Rabap-tin-5 with Rabex-5 is essential for exerting Rab5
effec-tor functions, this finding suggests that Rabaptin-5d
not merely binds Rab5, but similarly to Rabaptin-5,
can function as its effector
In summary, our data suggest that whereas
Rabaptin-5 is a bifunctional protein interacting with two
GTP-ases, Rab5 and Rab4, the Rabaptin-5d isoform lacks
this bifunctionality Our results indicate that
Rabaptin-5d is a Rab5 effector but unlike Rabaptin-5, does not
interact with the Rab4, presumably due to a disrupted
Rab4 binding site, and is thus unlikely to provide a link
to a Rab4-positive domain It is tempting to hypothesize
that selective recruitment of the d Rabaptin-5 isoform
by an early endosome would disfavor it moving along
the early recycling pathway This model should be
veri-fied experimentally, and if confirmed, it would provide
an example of membrane traffic regulation through
selective recruitment of a minor Rab effector variant
Experimental procedures
Plasmids
To construct Rab5, Rab5Q79L and
pPC97-Rab5S34N, the respective cDNAs were excised from
pLexA-Rab5, pLexA-Rab5Q79L and pLexA-Rab5S34N
[12] with EcoRI and NheI The cDNAs with filled-in EcoRI site were subcloned between XbaI and filled-in BamHI sites
in pBK-CMV vector (Stratagene, La Jolla, CA, USA), and subsequently excised and cloned between SalI and NotI sites in pPC97 vector [27] to obtained in-frame fusion with GAL4 DNA-binding domain (GAL4BD)
To construct pPC97-Rab4, pPC97-Rab4Q67L and pPC97-Rab4S22N, the respective cDNAs were excised from pLexA-Rab4, pLexA-Rab4Q67L and pLexA-Rab4S22N [14] with EcoRI and SalI and cloned between EcoRI and XhoI sites in pBK-CMV vector After digestion with SpeI, filling-in and self-ligation, the cDNAs were excised and cloned between the SalI and NotI sites in pPC97 vector to produce in-frame fusion with GAL4BD pPC86-Rabaptin-5 plasmid with a pPC86 backbone [27] for expression of the GAL4 transcription activating domain (GAL4AD) linked
to the full-length mouse Rabaptin-5 was described previ-ously [23] pPC86-Rabaptin-5d was constructed in a similar way
To obtain pPC97-Rabaptin-5 and pPC97-Rabaptin-5d, the respective cDNAs from Rabaptin-5 and pPC86-Rabaptin-5d were subcloned into the pPC97 vector between SalI and NotI sites To construct deletion mutants
of Rabaptin-5 in pPC86 vector, the Rabaptin-5 cDNA was subcloned from pPC86-Rabaptin-5 vector to pBlue-script SK II(+)plasmid (Stratagene) between SalI and NotI sites (plasmid pRn5) The resulting plasmid was used as a template to amplify cDNA fragments encoding amino acids 1–294 and 140–294 with the antisense primer 5¢-GTCTCA CATCAGCAAACGCT-3¢ As a sense primer, plasmid T7 primer or the primer 5¢-AGCAGGTCGACAGCACAGT GGGCACAGTAT-3¢ containing SalI site were used, respectively Amplified sequences were cloned between SalI and SmaI sites in the pBluescript SK II(+) vector, se-quenced, and subcloned into the pPC86 vector between the SalI and NotI sites pPC86 plasmid expressing amino acids 1–149 of Rabaptin-5 fused to GAL4AD was obtained by subcloning of the SalI-PstI 5¢-end fragment of Rabaptin-5 cDNA from pRn5 between the SalI and PstI sites of pBlue-script SK II(+) vector with sequential subcloning of the SalI-NotI fragment into the pPC86 vector
Deletion mutants of Rabaptin-5d and Rabaptin-5c in the pPC86 vector were constructed in a similar way To construct plasmids for expression of enhanced green fluor-escent protein (EGFP)-tagged Rabaptin-5 and Rabaptin-5d, the respective cDNAs were excised from the pRn5 and pRn5d plasmids with XhoI and NotI (the NotI site was blunted after digestion) and cloned between the XhoI and SmaI sites in pEGFP-C2 vector (Clontech Palo Alto, CA, USA) Plasmids for expression of N-terminally FLAG-tagged Rabaptin-5 and Rabaptin-5d were constructed in a following way The 5¢-end of the Rabaptin-5 coding region from amino acid 2 was amplified from the pRn5 template with the antisense primer used to construct deletion mutants of Rabaptin-5 in the pPC86 vector and the
Fig 9 Mapping of the Rab4 binding site in Rabaptin-5 using the
yeast two-hybrid protein–protein interaction assay Indicated
frag-ments of Rabaptin-5 (Rn5), Rabaptin-5d (Rn5d) or Rabaptin-5c
(Rn5c) fused to GAL4AD or GAL4AD alone (none) were
coex-pressed with GAL4BD fused to Rab4Q67L in yeast Y153 Amino
aids of Rabaptin-5 or its isoforms fused to GAL4AD are shown in
brackets, and deleted regions in Rabaptin-5 isoforms are presented
as thin lines LacZ reporter gene activation caused by interaction
between GAL4BD- and GAL4AD-fused proteins (b-gal) in yeast was
assessed by filter a b-galactosidase assay A similar assay with
GAL4BD alone as a bait revealed no activation of the LacZ reporter
gene (data not shown).
Trang 8SK II(+) vector using the internal HindIII site (plasmid
p5Rn5) The 3¢-end of the Rabaptin-5 coding region
inclu-ding the translation termination codon was amplified with
the sense primer
5¢-ACAAGGAATTCAGATTCAGGAA-3¢ and the antisense primer 5¢-GCAGTCTAGAATCCTGC
TATC-3¢ containing an XbaI site, and cloned between the
EcoRI and XbaI sites of the p5Rn5 multiple cloning site
using the internal EcoRI site in the amplified fragment
(plasmid p53Rn5)
All the amplified fragments of coding regions were
sequenced to confirm their authenticities The complete
coding regions of Rabaptin-5 and Rabaptin-5d were
obtained by cloning of ApaI-EcoRV internal fragments
from pRn5 and pRn5d, respectively, into the p53Rn5
plas-mid (plasplas-mids pRn5NF and pRn5dNF)
Finally, the entire coding regions were excised from the
pRn5NF and pRn5dNF plasmids with BglII and XbaI and
cloned into the pFLAG-CMV2 vector (Sigma, St Louis,
MO, USA) to obtain plasmids for expression of
N-termin-ally tagged Rabaptin-5 and Rabaptin-5d (plasmids
pFLAG-Rn5 and pFLAG-Rn5d) To construct plasmids
for expression of C-terminally FLAG-tagged Rabaptin-5
and Rabaptin-5d, the 3¢-end of the open reading frame of
Rabaptin-5cDNA was amplified with a sense primer
upstream of the unique EcoRV internal site and an
anti-sense primer that was designed to substitute the Rabaptin-5
translation termination codon for a coding triplet followed
by the FLAG epitope sequence, a translation
termin-ation codon and a NotI site The amplified fragment was
sequenced to confirm its authenticity The amplified
frag-ment was used to substitute the 3¢-ends in Rabaptin-5 and
Rabaptin-5d cDNAs in the pRn5 and pRn5d plasmids The
resulting cDNAs encoding C-terminally FLAG-tagged
proteins were then excised with SalI and NotI and cloned
between the XhoI and NotI sites of the pEGFP-N1 vector
(Clontech) to produce expression plasmids for
FLAG-tagged Rabaptin-5 and Rabaptin-5d (the EGFP coding
sequence was removed by digestion with XhoI and NotI)
The plasmid for expression of myc-tagged Rab5Q79L
was described previously [28] For construction of the
myc-Rab4Q67L expression vector, the myc-Rab4Q67L cDNA from
pPC97-Rab4Q67L was excised and cloned between the SalI
and NotI sites into a pBK-CMV vector engineered to
con-tain after the CMV promoter the fragment of the human
preproinsulin cDNA 5¢-untranslated region and ATG
codon (GeneBank Accession No NM_000207,
nucleo-tides 10–47) linked by an NcoI site with the NcoI-EcoRI
fragment from the pECT plasmid encoding His6 and myc
tags To construct plasmids for expression of glutathione
S-transferase (GST)-tagged Rab4 and Rab5, pGEX-Rab4
and pGEX-Rab5, the respective cDNAs were excised with
EcoRI and SalI from pLexA-Rab4 and pLexA-Rab5 and
Yeast two-hybrid methods The protein interaction assay was performed as described [29] In brief, the yeast strain Y153 was used to cotrans-form bait and prey plasmids, and transcotrans-formants were selec-ted by plating onto synthetic dextrose (SD) leucine and tryptophan dropout plate To evaluate HIS3 reporter gene trans-activation, yeast from a single colony was spotted onto SD medium lacking leucine and tryptophan, and sup-plemented or not with 25 mm 3-amino-(1,2,4)-triazole (3AT), and grown at 30C The HIS3 reporter gene trans-activation was monitored by the ability of yeast to grow on 3AT-containing medium or LacZ reporter gene trans-acti-vation was assessed in filter b-galactosidase assay
Cells and transfection BHK-21 cells were maintained in Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA, USA) supple-mented with 10% fetal bovine serum, 110 mgÆL)1 sodium pyruvate and 100 unitsÆmL)1 penicillin⁄ 100 lgÆmL)1 strep-tomycin For transfections, cells were plated at 100 mm cell culture dish at a density 1.2 106cells per dish For immu-nofluorescence microscopy, cells were plated on 10 mm cov-erslips in six-well plates at a density 200 000 cell per well The next day, cells were transfected with 0.25 lg of plasmid DNA per 0.75 lL Unifectin-56 liposome transfection rea-gent (kindly provided by A Surovoy, Shemyakin’s and Ovchinnikov’s Institute of Bioorganic Chemistry, Moscow, Russia) for a six-well plate, or with 1 lg of plasmid DNA per 3 lL Unifectin-56 liposome transfection reagent for a
100 mm dish
Antibodies The anti-Rabex-5 rabbit polyclonal antiserum [13] was a gift from M Zerial (Max-Plank Institute of Molecular Cell Biology and Genetics, Dresden, Germany) Mouse anti-(EGFP 2G7) monoclonal Igs were kindly provided by A Surovoy (Moscow, Russia) Mouse anti-(myc 9E10) mono-clonal Igs were used as supernatant from the respective hybridoma, and Alexa546-conjugated anti-mouse Igs (Molecular Probes, To detect the FLAG-epitope, rabbit anti-FLAG polyclonal antibodies (Sigma) were used following Alexa-488-conjugated antirabbit antibodies (Molecular Probes, Eugene, OR, USA) Rabbit polyclonal anti-(Rabaptin-5 Ig) antiserum was raised using a His6 -tagged mouse Rabaptin-5 fragment (amino acids 407–663)
as the immunogen To produce and purify recombinant protein, QIAexpressionist expression and purification system (Qiagen, Valencia, CA, USA) was used
Trang 9Confocal immunofluorescence microscopy
Twenty-four hours after transfection, cells on coverslips
were washed in phosphate-buffered saline (PBS) and fixed
with 3% (w⁄ v) paraformaldehyde Free aldehyde groups
were quenched with 50 mm ammonium chloride, and cells
were permeabilized with 0.05% (w⁄ v) saponin (Sigma)
After permeabilization, coverslips were washed with PBS
and incubated with primary antibodies diluted in PBS
con-taining 5% (w⁄ v) nonfat dry milk and 0.1% (w ⁄ v)
Tween 20 for 1 h After washing with PBS coverslips were
incubated as above with secondary antibody solution,
washed and mounted in Mowiol (EMD Biosciences, Inc.,
San Diego, CA, USA) Coverslips were examined with
Leica (Wetzlar, Germany) or Radiance 2100 (Bio-Rad,
Hemmel Hempstead, UK) confocal microscopes and images
were taken at 100 magnification and captured at
1024 1024 pixels or at 60 magnification and captured
at 512 512 pixels, respectively Montages of images were
prepared with use of photoshop 5.0 (Adobe, Mountain
View, CA, USA)
Preparation of cytosol
Cytosol for GST pull-down experiments was prepared as
described previously [12] with minor modifications
Thirty-six hours after transfection, cells from four 100 mm (diam.)
cell culture dishes were scraped into PBS, then pelleted and
homogenized in 400 lL of 250 mm sucrose, 10 mm sodium
phosphate, pH 7.2 by passages through a 27 gauge needle
attached to 1 mL syringe Cell breakage and nucleus
integ-rity were monitored by phase-contrast microscopy Nuclei
and debris were pelleted by centrifugation at 4000 r.p.m
for 10 min in an Eppendorf microcentrifuge The
postnu-clear supernatant was centrifuged at 60 000 r.p.m for 1 h
at 4C in a Beckman TLA-100 rotor to obtain cytosol
Cytosol for coimmunoprecipitation with Rabex-5 was
obtained in a similar way but cells were homogenized in a
buffer known to preserve the Rabaptin-5–Rabex-5
com-plex [13] [20 mm Hepes⁄ KOH, pH 7.2, 5 mm MgCl2,
1 mm dithiothreitol, 100 mm NaCl, 1 mm EDTA
contain-ing Protease Inhibitor Cocktail (Sigma)]
Recombinant proteins
GST-Rab4 and GST-Rab5 were produced in
Escheri-chia coliBL21 carrying pGEX-Rab4 and pGEX-Rab5
plas-mids Production and purification of GST-tagged proteins
were carried out on Gluthatione Sepharose 4B (APBiotech)
according to the manufacturer’s instructions Eluted
pro-teins were extensively dialyzed against 50 mm tris⁄ HCl,
pH 8.0, 135 mm NaCl, 1 mm EDTA followed by dialysis
against the same buffer containing 50% (v⁄ v) glycerol, and
stored at)20 C The final protein concentration was about
2 mgÆmL)1with purity over 95%
GST pull-down assay GST pull-down assays with GDP- or GTPcS-loaded GST– Rab4 and GST–Rab5 were performed essentially as des-cribed [11] Binding and washing were performed in batch, and cytosols prepared from two 100 mm (diam.) dishes of BHK-21 transiently transfected with plasmids for expression
of EGFP- or FLAG-tagged proteins were used to pull down EGFP- or FLAG-tagged 5 or Rabaptin-5d Before addition of cytosols to immobilized and nucleo-tide-preloaded GST-Rab4 or GST-Rab5, cytosols were diluted two-fold to adjust a final binding buffer composition After washing, SDS⁄ PAGE loading buffer was added to Sepharose beads, samples were boiled and loaded onto a 7.5% SDS⁄ polyacrylamide gel After separation, proteins were transferred onto Immobilon P poly(vinylidene difluo-ride) (PVDF) membrane (Millipore, Billerica, MA, USA), and membrane was immunoblotted with rabbit polyclonal anti-(Rabaptin-5 Ig) antiserum for EGFP-tagged proteins
or with monoclonal anti-(FLAG-M2) Igs (Sigma) for FLAG-tagged proteins Aliquots of cytosols were also immunoblotted with respective antibodies to monitor input protein contents
Coimmunoprecipitation and immunoblotting For coimmunoprecipitation of EGFP-tagged Rabaptin-5 or Rabaptin-5d with Rabex-5, cytosol was prepared from four
100 mm dishes Cytosol was incubated for 1 h with Protein
G Sepharose beads (Amersham Biosciences) precoated with
2 lg of mouse monoclonal anti-(EGFP 2G7) Igs, and washed three times with buffer used for cytosol preparation After addition of SDS⁄ PAGE loading buffer, samples were boiled and loaded onto a 7.5% SDS⁄ polyacrylamide gel After separation, proteins were transferred onto Immobilon P PVDF membrane (Millipore), and membrane was immuno-blotted with rabbit polyclonal anti-(Rabex-5 Ig) serum or rabbit polyclonal anti-(Rabaptin-5 Ig) serum Aliquots of cytosols were also immunoblotted with anti-Rabex-5 serum
or anti-Rabaptin-5 serum to monitor input protein contents
Acknowledgements
We thank Dr Marino Zerial (Max-Plank Institute of Molecular Cell Biology and Genetics, Dresden, Ger-many) for the anti-Rabex5 antiserum and Dr Andrey Surovoy (Shemyakin’s and Ovchinnikov’s Institute of Bioorganic Chemistry, Moscow, Russia) for anti-EGFP Igs and transfection reagents This work was supported by grants from the Russian Foundation for Basic Research and the Physical and Chemical Biology Program of the Russian Academy of Sciences E.K was partially supported by a FEBS Short-Term Travel Fellowship
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