Only a single nonconservative amino acid substitution between both H1.a allelic isoforms was detected by Edman degradation: glutamic acid present at position 117 in histone H1.a1 was rep
Trang 1Ewa Go´rnicka-Michalska1, Jan Pałyga1, Andrzej Kowalski1and Katarzyna Cywa-Benko2
1 Department of Genetics, Institute of Biology, Akademia S´wie˛tokrzyska, Kielce, Poland
2 Department of Animal Production, Faculty of Biology and Agriculture, Rzeszo´w University, Poland
In eukaryotes, the arrays of nucleosomes are linked
together by DNA to form chromatin fibers with a
diameter of 10 nm In bulk chromatin, one molecule
of histone H1 binds to a linker DNA sealing off two
turns of DNA around a core histone octamer Thus,
histone H1 plays a direct role in stabilizing
nucleo-somal and higher-order chromatin structures
A histone H1 family in higher eukaryotes is
repre-sented by several primary-sequence subtypes (nonallelic
variants) encoded by different, albeit closely related
genes [1,2] Disruption of a linker histone gene in both
protista and fungi demonstrated [3] that it is not
essen-tial for cell survival On the other hand, elimination of
three but not one or two H1 subtypes in mouse leads
to embryonic lethality due to about 50% reduction in
an H1 to nucleosome ratio [4]
It has been shown that the expression patterns of multiple H1 genes can change during development and tissue differentiation [3] The particular H1 variants can also be nonrandomly distributed in chromatin, differ in their turnover rates and in the extent of post-translational phosphorylation [5] Such differences suggest that linker histone variants could play a dis-tinct role in the regulation of chromatin activity Regu-latory function of the histone H1 may include a selective control of individual gene expression [3,6], inhibition of DNA repair by homologous recombina-tion [7], and transmission of apoptotic signals from the nucleus to the mitochondria [8], as well as targeting and activation of a major apoptotic nuclease DFF40⁄ CAD during terminal stages of apoptosis [9] Heritable developmental defects accompanied by
Keywords
allelic isoforms; chicken; genetic
polymorphism; histone H1; peptide
microsequencing
Correspondence
E Go´rnicka-Michalska, Department of
Genetics, Institute of Biology, Akademia
S´wie˛tokrzyska, ul S´wie˛tokrzyska 15;
25-406 Kielce, Poland
Fax: +48 41 3496292
Tel: +48 41 3496333
E-mail: egorn@pu.kielce.pl or
jpalyga@pu.kielce.pl
(Received 22 August 2005, revised 16
January 2006, accepted 19 January 2006)
doi:10.1111/j.1742-4658.2006.05147.x
Two allelic isoforms (H1.a1 and H1.a2) of histone H1.a were identified within two conservative flocks (R11 and R55) of Rhode Island Red chick-ens These proteins form three phenotypes: a1, a2 and a1a2 Birds with phenotype a1 were most common (frequency 0.825–0.980) while the a1a2 chickens appeared relatively rarely (0.017–0.175) The third phenotype a2, not detected in the tested populations, has only been revealed in progeny
of the purpose-mated a1a2 birds The polymorphism of histone H1.a was observed in all examined chicken tissues, so that the H1 preparations isola-ted from the lung, spleen, kidney and testis from the same individual exhib-ited identical phenotypes (a1, a2, or a1a2) This finding, together with inheritance data, supports the genetic nature of the H1.a polymorphism
As indicated by cleavages with a-chymotrypsin and protease V8, the H1.a1 and H1.a2 are two highly related proteins which differ within N-terminal part of their C-terminal tails Only a single nonconservative amino acid substitution between both H1.a allelic isoforms was detected by Edman degradation: glutamic acid present at position 117 in histone H1.a1 was replaced by lysine in histone H1.a2 Furthermore, using microsequencing techniques we have found a sequence homology between the N- and C-ter-minal parts of an unknown minor protein H1.y, present in the phenotype a2, and similar regions of histone H1.b
Abbreviations
2-ME, 2-mercaptoethanol; PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene difluoride; RBS, 0.01 M Tris ⁄ HCl, pH 7.4, 0.01 M NaCl, 0.003 M MgCl 2 ; NaCl ⁄ Cit, 0.15 M NaCl, 0.015 M sodium citrate; SDS, sodium dodecyl sulfate; TCA, trichloroacetic acid.
Trang 2changes in DNA methylation following a knockdown
of histone H1 genes in Arabidopsis [10], as well as
dis-turbances in chromatin structure leading to reduced
DNA methylation at specific regulatory sites and⁄ or
altered expression of specific genes in triple-H1 null
mouse embryonic stem cells [11] suggest that linker
histones could operate as epigenetic regulators of
higher-order chromatin conformations
Several studies support the idea that individual H1
subtypes could exhibit functional specialization For
example, histone B4, a Xenopus embryonic linker
his-tone, may confer a totipotency to embryonic cells [12]
while H1b, a specific subtype of mouse histone H1
complement, can restrain muscle differentiation by
forming a complex with a homeoprotein Msx1 [13] It
has been demonstrated [14] that H1.1, one out of seven
linker histones in Caenorhabditis elegans, took part in
chromatin silencing in a germ line In addition, this
protein was also able to enhance a telomeric position
effect in budding yeast [15] The authors suggested that
specific structural features of the H1.1 variant could
dedicate this protein to epigenetic silencing
Metazoan histone H1 proteins have a characteristic
tripartite structure which consists of a central globular
domain flanked by two basic N- and C-terminal tails
that are largely unstructured in a physiological
solu-tion [1] However, these disordered regions can assume
a secondary structure when interacting with DNA
[16,17] While the globular domain is the most highly
conserved region amongst somatic histone H1 variants,
the sequences of H1 tails are more variable, both in
length and amino acid composition, indicating that
they evolved by insertion or deletion and nucleotide
substitutions [18]
By expression of domain-swapping mutants, Brown
et al [6] determined that the central globular domains
of H10 and H1c were responsible for their differential
effects on gene expression Recently, using targeted
mutations, Hendzel et al [19] showed that the
C-ter-minal domain is required for a high affinity binding of
the histone H1 to chromatin Moreover, they
discov-ered differences in the binding affinity of the individual
histone H1 subtypes in vivo attributed to the
subtype-specific properties of the C-terminus [20]
Several nonallelic H1 variants may also be
represen-ted by two or more allelic isoforms in the chromatin
of several species [21–25] including humans [26]
Dif-ferences in the amino acid sequences between allelic
variants of a given H1 subtype were demonstrated in
only a few instances They were located mainly in
ter-minal domains of H1 molecule and usually originated
as a result of amino acid substitutions [25–28] or
inser-tions and deleinser-tions of repeated sequence motifs [21,24]
As in the case of the nonallelic variants [20], it seems that the alterations in molecular structure of allelic H1 isoforms may affect their affinity to DNA and chroma-tin, and their ability to interact with other chromatin proteins Most probably, this would result in certain phenotypic effects [24,29,30]
In our laboratory we have revealed a polymorphism for subtypes H1.a, H1.b, and H1.z in duck [23,31–33] and quail [34,35] In this work, in addition to well-known allelic variants of erythrocyte histone H5 [27] distributed differently in distinct chicken breeds [36],
we also observed two allelic isoforms of histone H1.a
in the chickens with a rare histone H1.a2 in a Rhode Island Red breed only Herein, we have demonstrated the genetic nature of the chicken histone H1.a poly-morphism and have employed a limited enzymatic cleavage and amino acid microsequencing to find a difference in the primary structure of the H1.a1 and H1.a2 allelic isoforms
Results
Variability of histone H1.a in Rhode Island Red chickens
Histone H1 proteins were extracted with a diluted per-chloric acid solution from erythrocyte nuclei of indi-vidual chickens and analyzed in an acetic acid–urea polyacrylamide gel As seen in Fig 1, the perchloric acid-soluble proteins predominantly contained a fast moving histone H5 and six slow migrating H1 sub-types They were designated according to the nomencla-ture of [37] as H1.a,.a¢,.b,.c,.c¢ and d Two erythrocyte isoforms of histone H1.a (H1.11 L), H1.a1 and H1.a2, were identified in the acetic acid–urea gel within Rhode Island Red chicken populations These variants form three phenotypes: a1, a2 and a1a2 (Fig 1) The animals with phenotype a1 possess a high level of a single pro-tein H1.a1 Histone H1.a from the birds with phenotype a1a2 was resolved into two well-separated bands, H1.a1 and H1.a2, with a slightly lower level of the H1.a2 isoform The third phenotype a2 has only been found following purposely mating the birds with phenotype a1a2 In the a2 individuals, in addition to a high level of the expected histone H1.a2, a low amount of either H1.a1 or H1.a1-like protein (H1.y) was discerned (Fig 1) The differences in the electrophoretic mobility between H1.a1 and H1.a2 in the acetic acid–urea gel (Figs 1 and 2) and a lack of such differences in the poly-acrylamide gel electrophoresis conducted in the presence
of SDS (Fig 3) seem to indicate that both proteins have a similar apparent molecular weight and can differ from each other in a net charge
Trang 3A phenotypic variability of histone H1.a was
detec-ted only in the Rhode Island Red chickens (Table 1)
In the R11 and R55 conservative flocks, we found two
phenotypes, a1 and a1a2 Birds with phenotype a1
were most common (frequency 0.825–0.980) while the
heterozygous a1a2 chickens were quite rare (frequency
0.017–0.175) Moreover, the frequency of the
pheno-type a1a2 was very similar in two different genetic
groups (R11 and R55) and varied widely between two
independent populations of the same conservative flock
R11 (Table 1)
A distribution of the phenotypes of erythrocyte
his-tone H1.a in a progeny from various matings of the
Rhode Island Red chickens is presented in Table 2 As
we were unable to detect animals with phenotype a2 in
all tested farm populations, we could only perform
three available types of crosses: a1· a1, a1a2 · a1a2,
and a1· a1a2 As expected, the third phenotype a2
was revealed after mating the a1a2 individuals In the
offspring of such parents, we found three categories of
phenotypes: a1, a1a2 and a2 in a ratio 1 : 2 : 1 The
data obtained from all crosses support the assumption
that a single gene with two co-dominant alleles is
responsible for the observed polymorphism of histone H1.a
The histone H1 preparations isolated from lung, spleen, kidney and testis of three Rhode Island Red
Fig 1 A comparison of three phenotypes of erythrocyte histone
H1.a in Rhode Island Red chickens Histone H1 proteins from
erythrocytes nuclei were resolved in acetic acid–urea
polyacryl-amide gel H1 and H5, histone H1 and H5; H1.a,.a¢,.b,.c,.c¢,.d,
his-tone H1subtypes; H1.a1, H1.a2, two isoforms of hishis-tone H1.a;
H1.y, H1.a1-like protein The phenotypes of histone H1.a were
(L), spleen (S), kidney (K) and testis (T) of chicken individuals with phenotype a1, a2 and a1a2 Proteins were separated in acetic acid– urea polyacrylamide gel All symbols are explained in the legend to Fig 1.
Fig 3 Concentration and purification of histone H1.a isoforms and H1.y protein from chicken erythrocyte nuclei by a preparative SDS– gel electrophoresis The bands containing H1.a and H1.y were excised from the gel, avoiding contamination with remnants of H1.a¢.
Trang 4chicken phenotypes were also resolved in the acetic
acid-urea gel (Fig 2) into six main subtypes [37],
which exhibited tissue specificity (Fig 2) A
poly-morphism of histone H1.a was observed in all
exam-ined chicken tissues (Fig 2) so that H1 isolated from
the lung, spleen, kidney and testis of the same
individ-ual exhibited identical phenotypes (a1, a2 or a1a2)
These results, together with inheritance data (Table 2),
support the genetic nature of the H1.a polymorphism
Limited proteolysis of H1.a isoforms and protein
H1.y
The stained protein bands containing appropriate
all-elic forms of the chicken erythrocyte histone H1.a or
protein H1.y were cut out from the acetic acid–urea
gel After concentration and purification by
prepara-tive sodium dodecyl sulfate (SDS) electrophoresis
(Fig 3), the proteins were recovered from gel strips by
electroelution and then precipitated from the solution
Samples of the H1.a and H1.y prepared in such a way
were suitable for further analysis of the protein
struc-ture
By using a limited digestion with a-chymotrypsin, we
revealed that the polymorphic isoforms of histone H1.a
differed in their C-terminal peptides (Fig 4A) As
his-tone H1 molecule is cleaved with a-chymotrypsin on
the C-terminal side of a single phenylalanine [32], a
limited a-chymotrypsin digestion of the chicken histone H1.a yielded two fragments [37]: a C-peptide that rep-resented the C-terminal part of the H1.a (residue 110–224) and a slightly shorter N-peptide from the N-terminus to Phe109 (Fig 5) In the acetic acid–urea gel, a relative migration of a-chymotrypsin generated
C1- and C2-peptides resembled that of the native pro-teins H1.a1 and H1.a2 (Fig 4A), while that of the N-peptides was identical regardless of the H1.a iso-form Moreover, molecular masses of the chymotryptic N-terminal peptides from H1.a1 and H1.a2 isoforms determined by an electrospray ionization mass spectro-metry were found to be identical except for a hetero-geneity within the H1.a1 N-peptide that appeared to be represented by a mixture of N-acetylated and N-un-acetylated species (results not shown) Collectively, these results clearly indicate that a putative difference
in the amino acid sequence between H1.a isoforms is located in the region spanning amino acid residue 110 (arginine) and the C-terminus (Fig 5) Similarly, one of the two peptides obtained by H1.y digestion with a-chymotrypsin migrated in the gel like H1.a1- or H1.a2-derived N-peptide, whereas the second one, with
an electrophoretic mobility similar to H1.a C-peptides, was almost undetectable in the gel (Fig 4A)
To demonstrate which part of the C-terminal domain
is responsible for putative amino acid differences between both isoforms, the H1.a1 and H1.a2 were
Table 1 A frequency of phenotypes and alleles of erythrocyte histone H1.a in conservative flocks of four chicken breeds Chickens from the same genetic group, R11, were bred in two different farms in Szczytno near De˛blin and Chorzelo´w near Mielec.
Breed and genetic group Total number of birds
Number of birds with
Frequency of alleles
Rhode Island Red
Table 2 Distribution of phenotypes of erythrocyte histone H1.a in progeny from various matings of Rhode Island Red R11 chickens.
Frequency of phenotypes in progeny (observed ⁄ expected)
v 2
Trang 5digested with Staphylococcus aureus protease V8 After
histone H1.a treatment with the protease V8 that can
preferentially cleave a peptide bond following glutamic
acid, only one distinct peptide band, identified as
C-ter-minal tail of H1.a (results not shown), was observed in
the gel (Fig 4B) In the C-terminal domain of H1.a
molecule there are only two glutamic acid residues at
positions 117 and 120 [37] Thus, a limited digestion of
H1.a with protease V8 generated a little shorter
C-ter-minal peptide ranging from residue 118 or 121 to the
C-terminus (Fig 5) Moreover, the remaining part of
the histone H1.a, with several glutamic acid residues
(positions 2, 10, 46, 57 and 78), would have to undergo
further cleavages into a number of smaller fragments
[37] undetectable in the gel As seen in Fig 4B, the
elec-trophoretic mobilities of the C-terminal peptides
obtained from H1.a1 and H1.a2 isoforms following
digestion with protease V8 were similar As we revealed
distinct differences in a migration between C1- and
C2-peptides derived from H1.a1 and H1.a2 proteins
after limited proteolysis with a-chymotrypsin, the
uni-form migration of protease V8-cleaved C-peptides
seemed to point out that differences between H1.a iso-forms were confined to a short region spanning from amino acid residue 110 to the glutamic acid at either the position 117 or 120 (Fig 5) As mentioned for H1.a isoforms, a protease V8 digestion of the protein H1.y also yielded only one peptide (Fig 4B)
Tandem mass spectrometry
To find more precisely which site could be altered between the two allelic H1.a isoforms, we applied a tandem mass spectrometry for a sequence prediction of the peptides generated by trypsin digestion As a sim-ilar set of peptides was detected in both proteins (results not shown) we were unable to reveal any meaningful alterations between the isoforms using this procedure
However, a primary structure of protein H1.y was partially revealed by the tandem mass spectrometric analysis of its trypsin products Besides five pep-tides present in almost all of the H1 subtypes, the trypsin digestion generated one peculiar peptide
high degree of similarity to histone H1.b (H1.03) (resi-dues 1–19) This 19-amino acid peptide was a part of
Fig 4 Limited proteolysis of H1.a1 and H1.a2 histones, and H1.y
(H1.a1-like protein) from chicken erythrocytes with a-chymotrypsin
(A) and Staphylococcus aureus V8 protease (B) The digestion
prod-ucts were resolved in the acetic acid–urea gel C 1 , C 2 , chymotryptic
C-terminal peptides generated from the H1.a1 and H1.a2,
respect-ively; N, chymotryptic N-terminal peptides produced from the
H1.a1, H1.a2 and H1.y; C, V8 protease C-terminal peptides
obtained from the H1.a1, H1.a2 and H1.y; H1, undigested histone
H1 subtypes.
Fig 5 The main peptides generated by a limited cleavage of the chicken erythrocyte histone H1.a isoforms with a-chymotrypsin and Staphylococcus aureus protease V8 H1.a1 and H1.a2, isoforms of histone H1.a; C and N, C- and N-termini of histone H1.a; (C1, C2 )-chym., chymotryptic C 1 - and C 2 -peptides generated from the H1.a isoforms; N-chym., chymotryptic N-peptides produced from the H1.a1 and H1.a2; C-V8, the possible protease V8 C-peptides cleaved from the H1.a Using a published sequence [37], we assumed that limited proteolysis of H1.a with the protease V8 cre-ated a C-terminal peptide running from residue 118 or 121 to the C-terminus The expected difference between H1.a isoforms is located at a short region spanning from Arg110 to the glutamic acid either at position 117 or 120.
Trang 6the N-terminal domain, the most variable and specific
region for each H1 subtype [37] As the individual ion
score for H1.y N-peptide was 75 and this value was
significantly higher than the threshold score of 45, it
strongly supports either the identity or very extensive
homology (P < 0.05) between this peptide and the
his-tone H1.b (data not shown)
Partial microsequencing of chymotryptic
C-terminal fragments
We also applied automated Edman degradation for a
partial sequence determination in the H1.a1 and H1.a2
proteins in the region affected by a mutation For this
purpose, the C-terminal fragments of histone H1.a
iso-forms produced by a-chymotrypsin digestion have
been analyzed by amino acid sequencing after
trans-blotting the proteins from the SDS–polyacrylamide gel
to a polyvinylidene difluoride (PVDF) membrane
Fig-ure 6 shows a partial sequence comparison of chicken
histone H1.a variants In a region spanning the
resi-dues 110–120 in both H1.a1 and H1.a2 we revealed 10
identical amino acids out of 11 residues so that the
all-elic isoforms differed only by one amino acid A
gluta-mic acid at the position 117 in the histone H1.a1 was
replaced by a lysine in the H1.a2 isoform The partial
histone H1.a2 sequence data was deposited in the
Uni-Prot Knowledgebase (UniProtKB, http://www.ebi
ac.uk) under the accession number P8451533
We also determined the first 11 amino acid residues
for the longer C-terminal chymotryptic peptide cleaved
off the chicken H1.y (Fig 6) The analyzed fragment
corresponded exactly to the beginning of the
C-ter-minal domain of the histone H1.b (residues 109–119;
with serine at position 6 and aspartic acid at position
8 of the peptide) and that of the histone H1.a2 (with
proline at position 6 and lysine at position 8 of the
peptide) As the protein H1.y was isolated from the
chicken phenotype a2 enriched in the isoform H1.a2, the bands used for a histone H1.y concentration were cut out from the acetic acid-urea gel in which H1.y and H1.a2 migrated close to each other (Fig 1) Further separation of H1.y protein from the histone H1.a2 could not be achieved by the preparative SDS–gel elec-trophoresis due to almost identical migration of both proteins (Fig 3) Therefore, we conclude that our his-tone H1.y preparation was sequenced as a mixture of the histone H1.y and an accompanying histone H1.a2 Thus, the automated Edman degradation of H1.y chymotryptic C-peptide revealed a sequence homology between the beginning of the C-terminal part of H1.y and that of H1.b (residues 109–119; Fig 6)
Discussion
Each of the six H1 genes (11L, 11R, 03, 0.10, 01 and 02) identified in the chicken genome [38] encodes for a different H1 subtype [37] Differences in amino acid sequences among H1 subtypes could be connected with their differential capacity for interaction with chroma-tin [1,20] In a current dynamic view [39], the H1 mole-cules, with a residence time on the nucleosome modulated by a phosphorylation, are continuously exchanged between chromatin binding sites in a ‘stop-and-go’ mode A specificity in amino acid sequence may also preferentially alter the chromatin residence time of a unique variant at a specific locus or chroma-tin region [20,39] Thus, a microheterogeneity in the primary structure may imply functional differences between distinct H1 subtypes The linker histone het-erogeneity can further increase due to a genetic poly-morphism and⁄ or post-translational modifications [36,40,41] In this study, two allelic isoforms (H1.a1 and H1.a2) of histone H1.a were identified within two conservative flocks (R11 and R55) of Rhode Island Red chickens Histone H1.a also exhibits a distinct intrapopulation variability in other avian species such
as duck [31] and quail [34] As in the chicken, the poly-morphic H1.a subtype from duck erythrocytes was rep-resented by two electromorphs, H1.a1 and H1.a2, with similar apparent molecular weights and different net charges [31] The genetic polymorphism of histone H1.a in Pharaoh quail was associated with a lack of this proteins in some birds or its occurrence at either
an intermediate or high level in other individuals [34]
In both flocks of Rhode Island Red chickens (Table 1), representing a typical multipurpose breed with a brownish red plumage, a frequency of allele a2 was quite low (0.01–0.09) Similarly, the allele a2, which was also rare or absent in most genetic duck groups, was enriched in the color-feathered duck
popu-Fig 6 Alignment of the first 11 amino acid residues in the
chymo-tryptic C-peptides obtained from chicken histones H1.a1, H1.a2 and
protein H1.y Chicken histone H1.a (residues 110–120) and H1.b
(residues 109–119) sequences designated with the asterisk
[Uni-Prot Knowledgebase (Uni[Uni-ProtKB) accession numbers P08287 and
P08285, respectively] are presented for comparison.
Trang 7lations [31] Surprisingly, the number of homozygous
a2 chickens was not reduced and the proportion of
other phenotypes did not differ from the expected
val-ues in the offspring of purpose-mated heterozygous
birds As the homozygous individuals a2 did not seem
to be less viable, it is likely that a lack of this
pheno-type in both conservative chicken flocks might be
either fortuitous or caused by breeding conditions The
changes in histone H1 allele frequencies were observed
in quail populations divergently selected for a high or
low reduction in body mass following transient
starva-tion [30] Only a1a1 homozygotes and a1a0
hetero-zygotes were detected among individuals of those
populations, while rare homozygotes a0a0 were found
exclusively in a control random mating population
[30] Thus, it was likely that a divergent selection in
quail could have acted against the allele a0 Berdnikov
et al [42] analyzed the influence of environment on the
histone H1 allele frequencies in Pisum sativum Using a
collection of 833 accessions of the cultivated pea
ori-ginating from different regions of the Old World they
revealed that the alleles of the specific H1 subtypes
(H1–5 and, possibly, H1–1) were subjected to
climatic-ally dependent natural selection under conditions of
primitive farming
The similar molecular weights and different net
charges of the chicken H1.a1 and H1.a2 suggest that
one of these proteins might have arisen as a result of
amino acid substitution or post-translational
modifica-tion In order to check whether phosphorylation is
responsible for H1.a heterogeneity, we performed a
digestion of H1 samples from the individuals differing
in the H1.a phenotype with alkaline phosphatase
(results not shown) As the enzyme treatment did not
change the patterns of histone H1.a migration, we
believed that phosphorylation is not responsible for
the observed polymorphism Thus, the most
convin-cing explanation of the intrapopulation variability of
H1.a is that the H1.a1 and H1.a2 represent allelic
pro-teins encoded by a gene with two codominant alleles at
a locus Previously, we detected [36] two allelic
electro-morphs (a and b) of the chicken erythrocyte histone
H5 A limited proteolysis with a-chymotrypsin and
subsequent microsequencing (unpublished results)
revealed that both polymorphic variants differed in
their N-terminal tails and therefore they resembled two
chicken H5 sequence variants Va and Vb with a
gluta-mine-to-arginine substitution at the position 15 [27]
Most of the allelic variants so far reported for the
polymorphic histone H1 subtypes differ in the N- or
C-terminal tails [23,24,27,32], though an amino acid
replacement in the globular domain was also detected
[25] Specific site cleavages in H1.a1 and H1.a2
pro-teins with a-chymotrypsin and protease V8 have shown that a variable region of the H1.a isoforms is located at the beginning of the C-terminal tail, between amino acid residue 110 and glutamic acid either at position 117 or 120 Relying on the sequence data for the chicken histone H1 subtypes [37] and their genes [38], a limited amino acid variability is apparent in the part of C-terminal tail adjacent to the globular domain In this region there are several conservative (N⁄ S, E ⁄ D, V ⁄ G) and nonconservative (V⁄ T, P⁄ S,
K⁄ L) substitutions between paralogous H1 subtypes including those involving charged residues
The C-terminal domain of histone H1 is presumably involved in the organization of the linker DNA and may be responsible for stabilization of condensed chro-matin fiber [19,43] Vila et al [16] have studied con-formational properties of a C-terminal peptide CH-1, which is placed adjacent to the central globular domain
in rodent histone H10 (residues 99–121), by a Fourier-transform infrared spectroscopy The authors showed the presence of inducible helical and b-turn (S⁄ TPKK) elements in this peptide in both trifluoroethanol solu-tion and in the complexes with DNA In the presence
of helical inducers, the helical region has a marked am-phipatic nature with all basic residues on one site of the helix and all hydrophobic residues on the other one [17] The amino acid substitutions in this H1 region may influence its binding to chromatin [19], including binding to specific chromatin proteins, for histone H1 C-terminal region may mediate protein–protein interac-tions [9,44] Lu and Hansen [43] demonstrated that the ability of the H10 to alter linker DNA conformation and to stabilize condensed chromatin structure is not distributed evenly throughout the entire domain The sequences that mediate folding were localized to two specific subdomains within C-terminal tail (97–121 and 146–169) Interestingly, the two H10 S⁄ TPKK motifs were found in each of the C-terminal subdomain (resi-dues 97–121 and 146–169) These sequences form b-turns that bind the minor groove of DNA [45]
A comparison of sequence data of the C-terminal region responsible for H1.a variability indicates that allelic isoforms of the chicken histone H1.a differ by only one amino acid residue At position 117, there is
a glutamic acid in histone H1.a1 and lysine in histone H1.a2 At the nucleotide level, a transition from G to
A in the first position of glutamic acid codons (GAA
or GAG) might have resulted in the replacement of Glu117 to Lys (codons: AAA or AAG) in H1.a2 Thus, a charge difference between both H1.a allelic isoforms was due to a nonconservative Glu117Lys substitution which could in turn explain a different mobility of H1.a1 and H1.a2 in the acetic acid–urea gel
Trang 8The amino acid sequence variation among different
paralogous H1 subtypes might influence a positive
charge of the protein and thereby affect the interaction
between negatively charged DNA and positively
charged C-terminal tails Recent studies have shown
[46,47] that a modulation of the coulombic interactions
between H1 and DNA by post-translational
modifica-tions or by amino-acid mutamodifica-tions is essential for gene
regulation Thus, the substitution of a negatively
charged amino acid (Glu) in the chicken H1.a1 to a
positively charged one (Lys) in the H1.a2 is an
essen-tial sequence alteration with possible functional
conse-quences The electrostatic DNA–histone interactions
are supplemented by the regulated interactions between
histones and effector proteins in the chromatin region
posed for gene transcription, as proposed by the
‘histone code’ hypothesis [48] Histone covalent
modifi-cations, especially on core histone N-tails, act
sequen-tially or in a combination to form a nucleosomal
‘epigenetic code’ that can differentially interact with
structural, enzymatic and⁄ or regulatory protein
com-plexes [48] As suggested [10], the members of the
his-tone H1 family which could also harbor epigenetic
markings [40] may act themselves as epigenetic
regula-tors Lately, some experiments seem to provide
evi-dence for functional specificity among H1 subtypes
[8,12,13,44]
Recently, Berdnikov et al [24] compared
quantita-tive traits in near isogenic lines of lentil and grasspea
carrying allelic variants of the most abundant subtype
H1-1 In this experiment, small (1–8%) but significant
differences have been revealed for some quantitative
traits such as: the mean number of seeds per pod,
mean seed mass, the flower fresh mass and the number
of ovules in a carpel These effects might have reflected
a direct association between histone H1 complement
and fitness, as evidenced for a Lys178Asn substitution
in the C-terminus of H1–5)1, a slow allelic variant of
pea histone H1–5 [25] that was most abundant in
geo-graphical region with a cold climate [42]
At present, the functional significance of allelic
vari-ation within the relevant H1 subtype is unknown
Although no satisfactory assay for assessing their role
in chromatin is currently available [26], we [30] and
others [42] observed a differential susceptibility of
H1 allelic variants to artificial and natural selection,
respectively, in response to environmental stimuli that
most likely might challenge a genetic buffering capacity
of Hsp90 chaperone complexes and⁄ or other capacitors
of phenotypic variation [49] Recent observation [50]
that Hsp90 facilitates loading linker histones to a
speci-fic binding and transporting protein NASP seems to
support a putative role of the chaperone in buffering
phenotypic effects of genetic variation in H1 histones under normal conditions
Experimental procedures
Animals
In this study we used Rhode Island Red chickens main-tained as the conservative flocks (R11 and R55) at the breeding poultry farm in Szczytno near De˛blin and in Chorzelo´w (R11) near Mielec, Poland The Cornish chick-ens were obtained from Poultry Research and Development Centre in Zakrzewo near Poznan´ All the remaining chicken breeds were from the farm in Szczytno near De˛blin Blood from wing vein of individual birds was separately collected into NaCl⁄ Cit solution (0.15 m NaCl, 0.015 m sodium citrate) containing 0.1 mm phenylmethylsulfonyl fluoride (PMSF) Further experiments were carried out using lung, spleen, kidney and testis from three 5-month-old indi-viduals of the Rhode Island Red R11 breed After quick decapitation, the tissues were immediately removed, perfused
or washed with ice-cold NaCl⁄ Cit solution supplemented with phenylmethylsulfonyl fluoride and stored at )20 C, but no longer than three weeks These experiments were car-ried out in accordance with the European Communities Council Directive of November 24 1986 (86/609/EEC)
Isolation of erythrocytes, nuclei and histone H1 extraction
All procedures were performed at 0–4C The blood mixed with NaCl⁄ Cit solution was centrifuged at 2000 g for 10 min (MPW-360, MPW Med Instruments, Warsaw, Poland) The supernatant with a layer of leukocytes was aspirated, and the erythrocytes were washed twice with NaCl⁄ Cit solution containing 0.5 mm phenylmethylsulfonyl fluoride The pellet
of red blood cells was stored at)20 C until used
Nuclei were isolated by one of two methods: (a) Erythro-cyte nuclei were prepared by a lysis in 0.1 m phosphate buffer, pH 7.0, containing 0.9% (w⁄ v) NaCl, 0.03% (w ⁄ v) saponin and 1 mm phenylmethylsulfonyl fluoride [28] (b) Nuclei from lung, spleen, kidney and testis were pre-pared by a sucrose-Triton X-100 method [51]
Histone H1 proteins were extracted from the washed nuclei first with 1 m and then with 0.5 m perchloric acid Protein from the combined supernatants were precipitated with 20% (w⁄ v) trichloroacetic acid, washed twice with aci-dified acetone (acetone⁄ concentrated HCl, 250 : 1, v ⁄ v) and acetone, and then air dried
Electrophoretic analysis
Histone H1 proteins were separated in an acetic acid–urea gel containing 15% (w⁄ v) acrylamide, 0.9 m acetic acid and
Trang 98 m urea and stained in Coomassie Blue R-250 [32] For
preparative SDS gel electrophoresis, approximately 18
stained gel fragments of the acetic acid–urea gel containing
H1.a isoforms and H1.a1-like protein (H1.y) were
sepa-rately incubated in adaptation buffer [2% (w⁄ v) SDS, 10%
(v⁄ v) 2-mercaptoethanol (2-ME), 10% (v ⁄ v) glycerol,
0.125 m Tris⁄ HCl, pH 6.8] The gel pieces were then
stacked in a well of one-dimensional 15% (w⁄ v)
polyacryl-amide gel containing 0.1% (w⁄ v) SDS [35]
Isolation of histone H1.a isoforms and protein
H1.y
The stained bands of the appropriate protein, purified and
concentrated during preparative electrophoresis, were
excised from the SDS gel to avoid neighboring
contamina-tions [35] Three protein bands of the same kind (H1.a1,
H1.a2 or H1.y) were pooled into glass tubes, and put over a
dialysis membrane Electroelution was performed in a buffer
containing 0.1% (w⁄ v) SDS, 0.025 m Tris, and 0.192 m
glycine Protein samples precipitated with 0.2 m KCl were
centrifuged at 10 000 g for 10 min (K24D, Janetzki, Leipzig,
Germany) Precipitated SDS was then removed by washing
the pellets with 20% (w⁄ v) trichloroacetic acid A small
amount of pellet remaining after centrifugation was washed
twice with acidified acetone and acetone, and dried
Digestion with a-chymotrypsin and protease V8
from Staphylococcus aureus
Histone H1.a isoforms and protein H1.y isolated by
elec-troelution were dissolved in: (a) 10 lL 0.05 m Tris⁄ HCl,
pH 8.0 and a-chymotrypsin (45 UÆmg)1, Serva
Electrophor-esis, Heidelberg, Germany) was added to a concentration
of 0.2 ngÆlL)1; (b) 10 lL 0.1 m Tris⁄ HCl, pH 7.5 and
pro-tease V8 (660 UÆmg)1, Sigma, St Louis, MO, USA) was
added to final enzyme concentration 7 ngÆlL)1 In both
cases, the proteolysis was performed at room temperature
for 15 and 45 min, respectively The reaction was stopped
by boiling the samples for 2 min Then appropriate volumes
of 8 m urea and concentrated acetic acid, 2-ME and
gly-cerol were added to receive 2.5 m urea, 0.9 m acetic acid,
5% (v⁄ v) 2-ME and 10% (v ⁄ v) glycerol in the reaction
solution The proteolytic degradation products were
resolved in the acetic acid–urea polyacrylamide gel
Identification of protein resolved in the gel
by mass spectrometry
The strips of the acetic acid–urea gel containing H1.a
iso-forms and protein H1.y, stained with Coomassie blue
R-250, were equilibrated with SDS-adaptation buffer Only
one gel piece per well was loaded into the wells of 15%
(w⁄ v) polyacrylamide gel containing SDS After
electro-phoresis, the protein bands were excised, in-gel reduced, S-alkylated and digested with modified trypsin (Promega, Madison, WI, USA) Tryptic peptides, washed out from the gels, were resolved by RP-HPLC using LC Packings (Sunnyvale, CA, USA) C18 precolumn (300 lm· 5 mm) and C18 column (75 lm· 15 cm) Peptides were eluted at 0.2 lLÆmin)1with a linear gradient of acetonitrile from 0%
to 25% (v⁄ v) in 25 min in the presence of 0.05% (v ⁄ v) for-mic acid The column outlet was coupled to an electrospray mass spectrometer (Micromass, Manchester, UK)
Molecular mass analysis was performed using the nano-Z-spray ion source of quadrupole-time of flight electrospray mass spectrometer working with the regime of data depend-ent on MS to tandem MS switch, allowing for a 3-s sequen-cing scan for each detected peptide The data were analyzed using the mascot program (http://www.matrixscience.com) All electrospray experiments were carried out at the mass spectrometry facility of the Institute of Biochemistry and Biophysics of the Polish Academy of Sciences in Warsaw, Poland
In-gel digestion, electroblotting and amino acid analysis
Stained bands containing concentrated and purified iso-forms of histone H1.a and protein H1.y were cut out from the SDS-preparative gel and soaked in adaptation buffer [0.1% (w⁄ v) SDS, 1 mm EDTA, 10% (v ⁄ v) glycerol, 0.125 m Tris⁄ HCl, pH 6.8] Next, the samples were loaded into the separate wells of 15% (w⁄ v) polyacrylamide gel containing 0.1% (w⁄ v) SDS and overlayered with 4 lL of the adaptation buffer containing 1 lgÆlL)1of a-chymotryp-sin (45 UÆmg)1, Serva Electrophoresis) After electrophor-esis, the proteins and polypeptides were electroblotted for 1.5 h at 90 V onto PVDF membrane (Roche, Mannheim, Germany) using 0.01 m 3-cyclohexylamino-1-propane-sulfonic acid, 10% (v⁄ v) methanol, pH 11 as a transfer buf-fer The PVDF membrane was washed in MilliQ (Millipore, Bedford, MA, USA) deionized water, stained with 0.1% (w⁄ v) Coomassie Blue R-250 in 40% (v ⁄ v) methanol and 1% (v⁄ v) acetic acid After destaining in 50% (v ⁄ v) meth-anol, the filter was rinsed in MilliQ deionized water and air dried Finally, the bands of chymotryptic C-peptides derived from H1.a isoforms and protein H1.y were excised from the blots and analyzed by automated Edman sequencing The N-terminal amino acid sequence analysis was per-formed on a gas-phase sequencer (Model 491, Perkin Elmer-Applied Biosystems, Foster City, CA, USA) at Bio-Center (Jagiellonian University, Krako´w, Poland) The phenylthiohydantoin derivatives were analyzed by on-line gradient high performance liquid chromatography on Microgradient Delivery System Model 140C equipped with Programmable Absorbance Detector Model 785 A (both from Perkin Elmer-Applied Biosystems)
Trang 10This work was supported by grant KBN 6P06D03520
from the Polish Ministry of National Education and
Science
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