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Mean corpuscular hemoglobin The results showed that mean white blood cell count WBC of depressed patients was 7.46 G/L, while in healthy people it was 6.86 G/L.. There was a statistical

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A STUDY ON THE CHANGES OF COMPLETE BLOOD COUNT

IN DEPRESSION PATIENTS

Pham Thi Thu 1 , Le Van Quang 2 , Nguyen Tat Dinh 3

SUMMARY

Objectives: To study the changes in some complete blood count indexes in depressed

patients Subjects and methods: Descriptive retrospective study Case group: 138 depressed

patients were treated in the Psychiatric Department, Military Hospital 103, discharged from January 1 st , 2019 to December 31 st , 2019 Control group: 164 healthy people giving from kidney donors at the Urology Department, Military Hospital 103 were discharged from January 1 st ,

2019 to June 30 th , 2020 Data were analyzed by SPSS 20 Results and conclusions: Among

depressed patients, there was an increase in the number of leukocytes, neutrophils and monocytes,

a decrease in the number of erythrocytes and hemoglobin (p < 0.05) Mean corpuscular volume and mean corpuscular hemoglobin was higher in the study group compared to the control group Meanwhile mean corpuscular hemoglobin concentration was lower than that in the control group Red cell distribution width in the case group was statistically higher than that in the control group (p < 0.05) The number and volume of platelets in depressed patients was smaller than in healthy people, statistical significance (p < 0.05)

* Keywords: Depression; Complete blood count

INTRODUCTION

Depression is a common chronic disease

in the world, and associated with a wide

range of impairment and secondary

morbidity [1] Today, there are many

studies on the pathogenesis of depressive

disorders in addition to the role of genes

and neurotransmitters, including the role

of inflammation Many studies show an

association between depression and

inflammatory factors such as cortisol,

CRP, interleukin, white blood cell count

[2] Several indicators of complete blood

count are important factors in the evaluation of inflammatory processes Some studies show an increase in the number of leukocytes and neutrophils,

a decrease in the percentage of lymphocytes in depressed patients [3, 4, 5]

In addition, there are the changes in other indexes of complete blood count in depression In Vietnam, there have not been any studies on this issue Therefore,

we carried out this study aiming: To study

the changes in some complete blood count indexes in depressed patients

1 Department of Psychiatry, Military Hospital 103, Vietnam Military Medical University

2 Naval Commando Brigade 126

3 Department of Psychiatry, Military Hospital 103, Vietnam Military Medical University

Corresponding author: Pham Thi Thu (thu41b@gmail.com)

Date received: 24/11/2020

Date accepted: 27/01/2021

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SUBJECTS AND METHODS

1 Subjects

- Case group: 138 depressed patients

treated in the Psychiatric Department of

Military Hospital 103 were discharged

from January 1st, 2019 to December 31st,

2019

+ Diagnosis of depression: According

to ICD 10 diagnotic criteria

+ Exclusion criteria: Patients with

combined severe somatic disease and

patients with white blood cell counts

above 10 G/L

- Control group: 164 healthy people giving kidney donors at the Urology Department of Military Hospital 103 were discharged from January 1st, 2019 to June

30th, 2020

2 Methods

* Study design: Descriptive retrospective

study

Complete blood count were tested by machine in the Hematology Department, Military Hospital 103 Venous blood had been taken after one day since patient was admitted to the hospital

* Data analysis: By SPSS 20.0 version

RESULTS AND DISCUSSION

1 Gender and age group

Table 1: Gender and age group

Gender

Age

> 0.05

The results showed that 60.1% of patients in the case group were male, this rate

in the control group was 61.0% Subjects in both groups were mainly less than

30 years old (47.8% in the case group and 45.7% in the control group) and from 31

to 50 years old

There were no statistically significant differences in gender and age between the case group and the control group (p > 0.05) In general population, depression was more common in women than in men (female/male ratio: 2/1) [1], however at Military Hospital 103, the majority of patients were military personnel, thus most of them were male and young, which was consistent with the gender and age group of donors

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2 The blood cell count indexes

Table 2: The blood cell count indexes

Mean corpuscular hemoglobin

The results showed that mean white

blood cell count (WBC) of depressed

patients was 7.46 G/L, while in healthy

people it was 6.86 G/L There was a

statistically significant higher number of

leukocytes among depressed patients

than healthy people (p < 0.05) The

number and percentage of neutrophils (N

and N%) in the case group was also

significantly higher than in the control

group (p < 0.05) The number of

lymphocytes (L) in two groups was not

statistically significant different, but the

percentage of lymphocytes (L%) in the

case group was statistically significant

lower than in control group (p < 0.05),

this was due to the fact that the increase

in the percentage of neutrophils reduces

the percentage of lymphocytes Similarly, the number of monocytes in the case group was significantly higher than in the control group (p < 0.05) but the increase

in the percentage was not statistically significant due to the greater increase in neutrophils This result is consistent with other findings [3, 4, 5] The white blood cell count is an important factor in the inflammatory evaluation According to the study by Demir S et al, in depressed patients there was an increase in the number of leukocytes and neutrophils, a decrease in the percentage of lymphocytes, but the number of lymphocytes did not have a statistically significant change compared with control group [4] A study

by Maes et al (1992) on 22 healthy people

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and 109 depressed patients found that

there was a significant increase in

leukocytes, neutrophils and monocytes in

depressed patients In addition, the number

of leukocytes, neutrophils, monocytes

was linearly associated with the severity

of depression [5]

Regarding the indexes of erythrocytes,

the results showed that the number of

erythrocytes and hemoglobin was

significantly lower in depressed patients

than in the control group (p < 0.05),

but no statistically significant difference

was found on hematocrit The mean

corpuscular volume and mean corpuscular

hemoglobin in the case group were

statistically higher than the control group

(p < 0.05), but the mean corpuscular

hemoglobin concentration in the case

group was lower than the control group

(p < 0.05) The indexes of mean

corpuscular volume, mean corpuscular

hemoglobin, mean corpuscular hemoglobin

concentration in both two groups were

within the normal range Vandoolaeghe E

et al conducted a study on 47 depressed

patients and 32 healthy people also found

that depressed patients had a statistically

significantly lower number of erythrocytes

than healthy people Hemoglobin and

hematocrit were also lower in the patient

group, but there was no difference in

mean corpuscular volume and mean

corpuscular hemoglobin According to the

author, this change was due to an

inflammatory response rather than a

deficiency of iron since the mean corpuscular

volume and mean corpuscular hemoglobin

did not decrease [3] According to Nemeth

E et al, inflammatory factors reduce

erythrocytes life, so the number of

erythrocytes in patients with inflammatory

processes is lower than in healthy group [6] Our results show that the red cell distribution width in the case group is statistically higher than the control group Red cell distribution width is the test to evaluate the change in the size and shape of red blood cells A study by Liqiang in 2017 also found that depressed patients had a higher red cell distribution width than healthy people [7]

Regarding the platelet indexes, the results showed that in the case group, the number of platelet as well as the mean platelet volume was significantly lower than the control group (p < 0.05) Platelets are a source of serotonin, a disruption of the serotonin system plays

an important role in the pathogenesis of depressive disorders In addition, platelets are variants in the inflammatory process The study by Liqiang also found that the case group had a statistically significantly larger platelet volume but found no statistically significant difference in platelet count The authors suggest that mean platelet volume may be useful marker of inflammation in depression [7] However, according to Sonmez, during the inflammatory process, activated platelets will stick to the white blood cells, focus on the inflammation site and aggregate, so that the number of platelets

in circulation can decrease [8] Several studies of platelet volume in chronic inflammatory diseases such as chronic periodontitis found that the mean platelet volume in the patient group was statistically significantly smaller than the control group [9] The author believes that large activated platelets have been aggregated and sticked, the rest of the platelets circulating in the blood had small

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size In addition, megakaryocytes in the

bone marrow is interfered by inflammatory

factors, resulting in produced small

platelets [9] A study on panic disorder

patients by Gul IG et al also found that the

patients with panic disorder had a smaller

platelet size than the control group The

authors suggest that abnormal serotonin

metabolism may lead to a decrease in

platelet size [10]

CONCLUSION

Through this study, we found that

many indicators of complete blood count

vary in patients with depressive disorder,

reflecting the association between

depressive disorders and inflammation In

particular, there was a statistically

significant increase in the number of

leukocytes, neutrophils and monocytes, a

decrease in the number of red blood cells

and hemoglobin among depressed patients

(p < 0.05)

Depressed patients had a higher mean

corpuscular volume and mean corpuscular

hemoglobin than the control group, mean

corpuscular hemoglobin concentration was

lower than the control group (p < 0.05)

Red cell distribution width in the case

group was statistically higher than the

control group (p < 0.05)

The number and volume of platelets in

depressed patients was significantly

smaller than in healthy people (p < 0.05)

REFERENCES

1 Kessler RC, Bromet EJ The epidemiology

of depression across cultures Annu Rev Pulic

Health 2013; 34:119-138

2 Haapakoski R, Mathieu J, Ebmeier KP,

Alenius H, Kivimäki M Cumulative

meta-analysis of interleukin-6 and 1β, tumour necrosis factor-α and C-reactive protein in patients with major depressive disorder Brain Behav Immun 2015; 49:206

3 Vandoolaeghe E, Nathalie De Vos, Demedts P, Wauters A, et al Reduced number of red blood cells, lowered hematocrit and hemoglobin, and increased number of reticulocytes in major depression as indicators

of activation of the inflammatory response system: Effects of antidepressant drugs Hum Psychopharmacol Clin 1999; 14:45-52

4 Demir S, Atli A, Bulut M, et al Neutrophil-lymphocyte ratio in patients with major depressive disorder undergoing no pharmacological therapy Neuropsychiatr Dis Treat 2015; 11:2253-2258

5 Maes M, Van Der Planken M, Steven

WJ, et al Leukocytosis, monocytosis and neutrophilia: Hallmarks of severe depression Journal of Psychiatric Research 1992; 26(2):125-134.

6 Nemeth E, Ganz T Anemia of inflammation Hematol Oncol Clin North Am 2014; 28(4):671-681

7 Liqiang Cai, Luoyi Xu, Lili Wei, Wei Chen Relationship of mean platelet volume to MDD: A retrospective study Shanghai Arch Psychiatry 2017; 29(1):21-29

8 Sonmez O, Sonmez M Rostrum role of

platelets in immune system and inflammation Porto Biomedical Journal 2017; 2(6):311-314.

9 Reddy GJ, Chandra MR, Gupta AA, et al Mean platelet volume as an imflammatory marker in patients with chronic periodontitis -

A pilot study Indian J Dent Adv 2018; 10(4):155-159

10 Gul IG, Erylmaz G, Ozten E, Sayar

GH Decreased mean platelet volume in panic disorder Neuropsychiatr Dis Trea 2014; 10:1665-1669

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