AATs regenerate the carbon skeletons Keywords aspartate aminotransferase; Bacillus subtilis; conserved active residues; kinetic parameters; protein sequence analysis Correspondence X.-W.
Trang 1aspartate aminotransferase from Bacillus subtilis B3
Hui-Jun Wu1,*, Yang Yang1,*, Shuai Wang2,*, Jun-Qing Qiao1, Yan-Fei Xia1, Yu Wang1,
Wei-Duo Wang1, Sheng-Feng Gao1, Jun Liu1, Peng-Qi Xue1and Xue-Wen Gao1
1 Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management
of Crop Diseases and Pest Insects, Ministry of Agriculture, China
2 Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China
Introduction
Aspartate aminotransferases (AAT; EC 2.6.1.1)
cata-lyze the reversible reaction of transamination between
four- and five-carbon dicarboxylic amino acids and
the corresponding a-keto-acids by a ping-pong, bi-bi
mechanism, with pyridoxal 5¢-phosphate (PLP) as an
essential cofactor [1] The enzyme plays a key role in
the metabolic regulation of carbon and nitrogen
metabolism in all organisms [2] In eukaryotes, AAT
along with malate dehydrogenase comprise a system
(i.e the malate-aspartate shuttle) for transporting reducing equivalents across organellar membranes [3]
In prokaryotes, AAT represents a central enzyme in metabolism of the Krebs citric acid cycle intermedi-ates For example, AAT converts newly-formed organic nitrogen to the nitrogen carriers, Glu and Asp, and the formation of Asp is used to generate several essential amino acids such as Asn, Met, Thr, Lys and Ile AATs regenerate the carbon skeletons
Keywords
aspartate aminotransferase; Bacillus subtilis;
conserved active residues; kinetic
parameters; protein sequence analysis
Correspondence
X.-W Gao, Department of Plant Pathology,
College of Plant Protection, Nanjing
Agricultural University, Key Laboratory of
Monitoring and Management of Crop
Diseases and Pest Insects, Ministry of
Agriculture, Nanjing 210095, China
Fax: +86 25 84395268
Tel: +86 25 84395268
E-mail: gaoxw@njau.edu.cn
*These authors contributed equally to this
work
(Received 4 December 2010, revised 20
January 2011, accepted 11 February 2011)
doi:10.1111/j.1742-4658.2011.08054.x
In the present study, we report the identification of a new gene from the Bacillus subtilisB3 strain (aatB3), which comprises 1308 bp encoding a 436 amino acid protein with a monomer molecular weight of 49.1 kDa Phylo-genetic analyses suggested that this enzyme is a member of the Ib subgroup
of aspartate aminotransferases (AATs; EC 2.6.1.1), although it also has conserved active residues and thermostability characteristic of Ia-type AATs The Asp232, Lys270 and Arg403 residues of AATB3 play a key role
in transamination The enzyme showed maximal activity at pH 8.0 and
45C, had relatively high activity over an alkaline pH range (pH 7.0–9.0) and was stable up to 50C AATB3 catalyzed the transamination of five amino acids, with L-aspartate being the optimal substrate The Km values were determined to be 6.7 mM for L-aspartate, 0.3 mMfor a-ketoglutarate, 8.0 mMforL-glutamate and 0.6 mMfor oxaloacetate A 32-residue N-termi-nal amino acid sequence of this enzyme has 53% identity with that of Bacillus circulansAAT, although it is absent in all other AATs from differ-ent organisms Further studies on AATB3 may confirm that it is poten-tially beneficial in basic research as well as various industrial applications
Database The nucleotide sequence data have been deposited in the GenBank database under accession Numbers AY040867.1
Abbreviations
AAT, aspartate aminotransferase; PLP, pyridoxal 5¢-phosphate.
Trang 2(a-ketoglutarate) for further primary nitrogen
assimi-lation [4]
AATs from many species have been classified into
the aminotransferase family I and then divided into
two subgroups, Ia and Ib, on the basis of their amino
acid sequences [5,6] The Ia subgroup contains AATs
from eubacteria and eukaryotes, such as Escherichia
coli, yeast, chickens and pigs, whereas Ib includes
those from thermophilic eubacteria and
thermoacido-philic archaebacteria, such as Thermus thermophilus
HB8 [6], Bacillus sp YM-2 [7] and Rhizobium meliloti
[8] More recently, a novel prokaryote-type AAT was
identified in plants belonging to the Ib subfamily in
eukaryotic organisms [2,9] The amino acid sequence
identities between subgroups Ia and Ib are only
15% Up until now, the most extensively
investi-gated AATs, with studies reported on their structure
as well as their function, are those from subgroup Ia,
whereas much less is known about AATs from
sub-group Ib Recently, the 3D structures of the subsub-group
Ib AATs from T thermophilus, Phormidium lapideum
and Thermotoga maritima were solved, showing that
the structures of the enzymes in subgroups Ia and Ib
are very similar [10–12] and that the active site residues
are well-conserved [6]
X-ray crystallographic studies in conjunction with
site-directed mutagenesis experiments have elucidated
the function of several conserved active residues of
AAT The Tyr70 is hydrogen bonded to the
phos-phate group of the co-enzyme PLP and stabilizes the
transition state [13] The Asn194 and Tyr225 residues
regulate the electron distribution through
hydrogen-bonding to O (3¢) of the co-enzyme PLP [14] Asp222
serves as a protein ligand tethering the co-enzyme in
a productive mode within the active site and
stabi-lizes the protonated N(1) of the co-enzyme to
strengthen the electron-withdrawing capacity of the
co-enzyme [15] The active site Lys258 transfers a
proton from the amino acid substrate to the cofactor
and forms an internal Schiff base with the cofactor
[16] Arg292 of the large domain in subgroup Ia
AAT recognizes the distal carboxyl groups of
dicarb-oxylate substrates [17]; however, this residue is not
found in the corresponding regions of subgroup Ib,
and the Lys109 residue performs this function instead
in subgroup Ib [18] Arg386 of the small domain
binding the a-COO) of the substrate plays a key role
in the activity of the enzyme [19,20] The functions
of the above-mentioned conserved active residues
were all identified by using the AAT from E coli as
the template, except for that of the Lys109 residue in
subgroup Ib, which was determined from the AAT
of T thermophilus
In Bacillus spp., AAT plays a very important role in the Krebs cycle, which synthesizes aspartate from oxaloacetate and is also involved in the synthesis of several essential amino acids [21] AATs have been iso-lated and characterized from several Bacillus spp In
B subtilis 168, the AAT is encoded by the aspB gene, which appears to be constitutively expressed [22] However, there are four other putative AATs in B sub-tilis168 based on whole genome analysis The AAT from alkalophilic Bacillus circulans contains an addi-tional N-terminal sequence of 32 amino acid residues, which functions to stabilize the structure over a wide
pH range and to prevent aromatic fluorophores from quenching by water [23] A preliminary X-ray structure
of the AAT from Bacillus sp YM-2 has been obtained [7] More recently, aminotransferases were divided into six subgroups and classified from B subtilis as members
of the If subgroup instead of the Ia subgroup [24] However, the generally accepted view is that AAT from
B subtilisis a member of the Ib subgroup
In the present study, a new gene aatB3 (accession number AY040867) encoding an AAT was cloned from the B subtilis B3 strain and analyzed phylogenetically
We also describe the expression in E coli and charac-terization of the recombinant enzyme by determining the optimum pH and temperature, substrate specifici-ties, kinetic parameters and the active-site residues
Results
DNA and protein sequence analysis The aatB3 gene and its regulatory element within a
3642 bp genomic region of B subtilis B3 were previ-ously sequence (accession number AY040867) [25] By analysis using software available online (as described in the Materials and methods), the sequence of the aatB3 gene was shown to comprise 1308 bp, including an ATG initiation codon and a TGA termination codon The G+C ratio of the ORF is 48.6%, which is 2% and 6% higher than the genomic G+C ratio of Bacil-lus amyloliquefaciensFZB42 (46.4%) and B subtilis 168 (43.5%) [26], respectively The deduced 436 amino acid product of aatB3 was predicted to have a molecular weight of 49.1 kDa, which is slightly lower than the value obtained on SDS⁄ PAGE ( 55 kDa) This differ-ence is the result of an additional 38 amino acid sequence including a 6· His tag fused to the N-termi-nus of AATB3 The calculated isoelectric point of AATB3 is 5.4 The putative promoter and ribosomal binding site regions were found upstream of the aatB3 gene The promoter has a typical )35, )10 and transcription start site, and there is a rho-independent
Trang 3transcription terminator flanking the stop codon of the
aatB3gene
The amino acid sequence of AATB3 showed 97–
98% identities with the putative AATs from other
B subtilis strains, although their enzymatic activities
have not been identified From the protein sequence
alignment of AATB3 and ATTs from several other
organisms (Fig 1), the AATB3 showed 56% identity
with B circulans AAT, and 16% and 14% with
Bacil-lus sp YM-2 and T thermophilus HB8 AATs,
respec-tively The latter two AATs belong to subgroup Ib
[5,6], although AATB3 showed 12% and 10%
identi-ties, respectively, with the E coli and pig cytosolic
AATs, which belong to subgroup Ia [5,6] Therefore,
based on the results described above, it appeared that
the AATs from B subtilis and B circulans should
belong to subgroup Ib
Expression and purification of AATB3 and its
mutants
To produce recombinant AATB3 and the three mutant
proteins, the aatB3 gene and its mutants were expressed
in E coli The recombinant proteins were purified by a
single chromatographic step using Ni2+-nitrilotriacetic
acid metal-chelating affinity chromatography as
described in the Materials and methods The purified
enzyme and three mutants each migrated as a
single band on SDS⁄ PAGE with a molecular weight of
55.0 kDa (Fig 2A), which is identical to the
calcu-lated value The sizes of the AATB3 protein and its
mutant proteins were slightly larger than the natural
forms (49.1 kDa) as a result of the additional 38 amino
acids, including a 6· His Tag sequence for affinity
chromatography fused to the N-terminus
Activities and functions of AATB and its mutants
To determine whether this new AAT from B
subtil-isB3 might also have AAT activity, the enzymatic
activity of the recombinant AATB3 expressed and
purified from E coli was analyzed Native PAGE
anal-ysis showed that the wild-type AATB3 had AAT
acti-vity when l-aspartate and the a-ketoglutarate were
used as amino donor and acceptor, respectively
(Fig 2C) In the paper chromatography analysis of
amino acids (Fig 3), the AATB3 also demonstrated
the ability to transfer the a-amino of the l-tryptophan
to a-ketoglutarate and oxaloacetate to produce
l-glu-tamate (Fig 3A) and l-aspartate, respectively
(Fig 3B) The results of the spectrophotometry
analy-sis showed that AATB3 also has weak l-tyrosine and
l-phenylalanine aminotransferase activities (Table 1)
To confirm which residues play key roles in the interaction between B subtilis B3 AAT and PLP, the Asp232 and Lys270 residues (corresponding to Asp222 and Lys258 in E coli AAT) were replaced with Asn and His using site-directed mutagenesis to obtain the mutants D232N and K270H, respectively The Asp232fi Asn replacement led to a loss of the nega-tive charge at position 232, and the Lys270fi His replacement introduced an imidazole ring into the enzyme and changes the structure of the enzyme No enzymatic activities were determined on native gels for the D232N and K270H mutant enzymes (Fig 2C), which is consistent with the spectrophotometry
Fig 1 Alignment of sequences of AATs Alignment was per-formed using CLUSTAL X [29] B.B3, B subtilis B3; B.circ., B circu-lans; B.YM, Bacillus sp YM2; T.th., T thermophilus HB8; cPig, pig cytosolic Gaps in the alignment are shown by gray dashes Identi-cal residues are shown in black; similar residues are shown in gray.
Trang 4analysis These two mutants also lost their
transamina-tion ability when using l-Trp and l-Phe as amino
donors (data not shown) To determine the exact role
of Arg403 (corresponding to Arg386 in E coli AAT)
in B subtilis B3, the R403Y mutant enzyme was con-structed The Arg403fi Tyr replacement disrupted the PLP-Asn194-Arg403 hydrogen-bond linkage system and changed the conformation of the active center of the enzyme The enzyme activity analysis showed that the R403Y mutant also lost transamination activity (Fig 2C) These results showed that the Asp232, Lys270 and Arg403 residues of B subtilis B3 AAT play key roles in transamination
Comparison and alignment of AAT sequences
To confirm the exact contributions of the Asp232, Lys270 and Arg403 residues to the function of B sub-tilisB3 AAT, the deduced amino acid sequence was compared with the five AATs identified from B circu-lans, pig cytosolic, E coli, T thermophilus HB8 and Bacillussp YM-2 The alignment results revealed 19 invariant amino acids in these six AATs (Fig 1) Among these conserved residues, the Tyr70, Asn194, Asp222, Tyr225, Lys258 and Arg266 residues in E coli AAT (numbered on the basis of the pig cytosolic AAT) are involved in the binding of PLP, which acts
as the co-enzyme [19,27] The Asp232 and Lys270 resi-dues in B subtilis B3 AAT correspond to Asp222 and Lys258, respectively, in E coli AAT Together with
Fig 2 Purification and functional analysis of the recombinant
wild-type (WT) and mutant AATB3 enzymes (A) Aliquots of purified
enzyme for the wild-type and each AATB3 mutant were separated
by SDS ⁄ PAGE and stained with Coomassie Brilliant Blue (B)
Aliqu-ots of purified enzyme for the wild-type and each AATB3 mutant
were separated by native PAGE and stained with Coomassie
Bril-liant Blue (C) Native PAGE gel was stained with Fast Blue in
accor-dance with the method described by de la Torre et al [9].
Fig 3 Detection of L -tryptophan aminotransferase activity using
paper chromatography of amino acids (A) the a-ketoglutarate was
used as the amino acceptor; L -Glu, standard L -Glu; L -Try, standard
L -Try; 1-3, reaction sample (B) The oxaloacetate was used as the
amino acceptor; L -Asp, standard L -Asp; L -Try, standard L -Try; 1–3,
reaction sample.
Table 1 Activity of purified AATB3 towards different amino acids and oxo acids The reaction was performed at 25 C for 20–40 min The activity was measured as described in the Materials and methods.
Concentration (m M )
Relative activity (%) Amino donor a
Amino acceptor b
a
The AAT from B subitilis B3 showed relative high activity toward
L -aspartate and L -glutamate, although the activities were very weak toward three aromatic amino acid aminotransferases ( L -tryptophan,
L -tyrosine and L -phenylalanine) Therefore, 30 m M was used for
L -aspartate and L -glutamate, and 6 m M for the three aromatic amino acid substrates a-ketoglutarate (10 m M ) was used as amino group acceptor except for the oxaloacetate (10 m M ) used for L -glutamate The activity of L -aspartate was adjusted to 100.b30 m M L -aspartate was used as amino donor for a-ketoglutarate, and 30 m M L -gluta-mate was used as amino donor for oxaloacetate The activity of a-ketoglutarate was adjusted to 100.
Trang 5analysis of the activities of the mutants D232N and
K270H (Fig 2C), we concluded that Asp232 in B
sub-tilisB3 AAT, which corresponds to Asp222 in E coli
AAT [15], is the residue that enhances the function of
the enzyme-bound co-enzyme PLP The Lys270 residue
of B subtilis B3 AAT serves the same function as
Lys258 in E coli AAT, which binds to PLP and forms
an internal Schiff base [16]
The conserved residues Asn194 and Arg386 in
E coli AAT participate in substrate binding [14,20],
which correspond to Asn199 and Arg403, respectively,
in B subtilis B3 AAT The loss of transamination
activity of the R403Y mutant confirmed that the
B subtilisB3 AAT utilizes the Arg403 residue to bind
the a-COO) of the substrate, which is similar to the
role of Arg386 in E coli AAT The Arg292 residue,
which is the invariant residue in the subgroup Ia AATs
[17] identified in the primary structure of B subtilis B3
and B circulans AATs, interacts directly with the
dis-tal carboxyl groups of dicarboxylate substrates
(Fig 1) However, this residue is not found in the
cor-responding regions of subgroup Ib By contrast, the
conserved active residue Lys109 in subgroup Ib carries
out the function of recognizing the substrates as does
the Arg292 residue in subgroup Ia [18] From the
alignment, the Thr109 was shown also to be conserved
in B subtilis B3, B circulans, E coli and pig cytosolic
AATs, and the Trp140 invariant among the six AATs
(Fig 1) These two residues provide hydrogen bonds
to the phosphate group and distal carboxyl group of
the substrate [27,28]
Molecular phylogeny
To examine the phylogenetic relationship of this new
bacteria gene with AAT genes from plants, animals,
protozoa, eubacteria and archeabacteria, a phylogram
was constructed using the Neighbor-joining method
with 44 full-length AAT amino sequences from
Gen-Bank As shown in Fig 4, the AATs were divided into
six main branches: animal mitochondrial, animal
cyto-plasmic, plant mitochondrial, plant cytoplasmic and
the two branches in bacteria The AAT from B
subtil-isB3, clustering together with the AAT from B
circu-lans, is in the large branch of bacterial AATs From
the phylogenetic tree analysis, the AATs from different
organisms can also be divided into two major
sub-groups according to the classification system
estab-lished by Jensen and Gu [5] The Ia subgroup contains
eubacterial and eukaryotic AATs, including enzymes
from E coli, Haemophilus influenzae, animals and
plants The Ib subgroup consists almost exclusively of
AATs from prokaryotes, including AATs from
proto-zoa, archaebacteria and bacteria Interestingly, plants also have Ib subgroup-prokaryote-type AATs [2,9] Although the AATs from B subtilis B3 and B circu-lans belong to the Ib subgroup in our analysis, these new AATs show significant differences from other Ib subgroup-type AATs They occupy a small separate branch at a far phylogenetic distance from AATs belonging to another large branch of the Ib subgroup From the homology analysis, the identity between the two AATs from B subtilis B3 and B circulans was
56%, and the AAT from B subtilis B3 showed rela-tively high identity ( 19%) with the AAT from Syn-echocystis sp compared to other AATs from the Ib subgroup
Enzyme specificity and kinetics parameters The purified AATB3 was optimally active at 45C (at
pH 7.2), and more than 80% of the maximum activity was retained in the temperature range 25–55C (Fig 5A) After incubation at 50C for 30 min, the enzyme had more than 85% of the maximum activity (Fig 5B) When incubated at 60C for 15 min, the enzyme also had 65% activity, although increasing the treatment time to 30 min caused the enzyme to lose almost all activity Above 65C, the stability of the enzyme decreased rapidly (Fig 5B) The optimal pH for the enzyme activity was pH 8.0 at the optimal tem-perature (45C) (Fig 5C) The enzyme activity over the pH range 7.0–8.6 was more than 80% of the maxi-mum activity From these results, we demonstrated that AATB3 tended to have relatively high activity and stability in alkaline environments
Table 2 summarizes the effect of some metal ions on the activity of the purified aminotransferase At a low concentration (1 mm), Cu2+ and Mn2+ could inhibit the activity of the purified aminotransferase, and other metal ions had no remarkable effects, although Ca2+ and Co2+ could promote the reaction to some extent Partial inhibition was observed in the presence of some metal ions at 10 mm, and the order of the ions by enzyme inhibitory activity was Zn2+>Cu2+>Mg2+
>Mn2+ It could be concluded that the enzyme is not metal ion-dependent because EDTA had no inhibitory
or stimulatory effects on the activity (Table 2)
AATB3 showed transamination activity between various amino acids and a-ketoglutarate (Table 1), with l-aspartate being the best substrate Aromatic amino acids such as l-tryptophan, l-tyrosine and
l-phenylalanine were weakly active as amino donors, and the activity of transamination activity toward
l-tryptophan was relatively higher than the other two residues
Trang 6To further characterize the enzyme, the kinetic
parameters Km, Vmax and kcatwere determined for the
purified AATB3 Values for Km and Vmax for both
amino donors (l-aspartate and l-glutamate) and
ac-ceptors (a-ketoglutarate and oxaloacetate) were
calcu-lated from the double-reciprocal plots The Km values
of AATB3 were 6.7, 0.3, 8.0 and 0.6 mm for
l-aspar-tate, a-ketoglutarate, l-glutamate and oxaloacel-aspar-tate,
respectively For the amino donors, AATB3 showed
more affinity for l-aspartate than l-glutamate,
whereas, for the amino acceptors, this enzyme had
more affinity for a-ketoglutarate (Table 3) The
calcu-lated Vmax for l-aspartate, a-ketoglutarate,
l-gluta-mate and oxaloacetate were 0.23, 0.21, 0.07 and
0.11 mmÆmin)1, respectively (Table 3) The kcat⁄ Km
ratios listed in the Table 3, which represent the
cata-lytic efficiency, show that the enzyme had relative
higher catalytic efficiency for oxo acids than for amino
acids The enzyme variants D232N, K270H and
R403Y were almost inactive (Fig 2C), and therefore
no kinetic parameters could be determined
Discussion
AATs that catalyze the tricarboxylic acid cycle inter-mediates to amino acids have been studied in a variety
of organisms These enzymes play a key role in aspar-tate catabolism and biosynthesis as well as in linking carbon metabolism with nitrogen metabolism In the present study, we cloned and characterized such an AAT from the B subtilis B3 strain This enzyme con-sists of 436 amino acid residues and is encoded by the aatB3 gene We found the typical promoter and termi-nator regions upstream and downstream, respectively,
of this new gene
To examine explicitly the phylogenetic relationship between the AATB3 and other AATs from different organisms, a phylogenetic tree was constructed using
Fig 4 Phylogenetic tree of AATs from dif-ferent organisms The phylogenetic tree was constructed with full-length AAT amino acid sequences using the Neighbor-joining method of MEGA 4.0 Bootstrap values are expressed as percentages of 1000 replica-tions Bar 0.1 sequence divergence c, cyto-solic; ch, chloroplastic; cy, cytoplastimic; p, plastidic; m, mitochondrial GenBank acces-sion numbers of the AATs are shown The black circle represents the branch of AAT from B subtilis B3 and B circulans; the black triangle shows the prokaryote-type AATs from plants.
Trang 7previously characterized AAT sequences from animals,
plants and prokaryotes The AATs from B subtilis B3
and B circulans clustered together with other bacterial
AATs and appeared to be more closely related to the
Ib-type of bacterial AATs than to the Ia-type of other
bacterial AATs (Fig 4) However, AATB3 showed low
identify with AATs from the Ib subgroup, and the
highest identity was only 19% compared to AAT
from Synechocystis sp (Ib subgroup)
Multiple alignments, which were built using AATs
of distant species, clearly show that most of the
resi-dues interacting with the PLP and the substrates
[27,29] are conserved in AATB3 (Fig 1) From this
comparison, the AATB3 tends to have more conserved
active residues that belong to the Ia subgroup but do
not exist in the Ib subgroup For example, the Gly38,
Thr109 and Arg292 residues (numbered on the basis of
the pig cytosolic AAT), which are conserved in AATB3 and Ia subgroup AATs, are not found in the
Ib subgroup AATs These three residues are all involved in the interaction with the substrate [27,28], especially the Arg292 residue, which plays a key role
in recognizing the distal carboxylate of the substrate [17] In subgroup Ib, the same role appears to be car-ried out by Lys109 [18] Therefore, AATB3 is more similar to the AATs from the Ia subgroup than the Ib subgroup in structure
We used site-directed mutagenesis to determine the exact role of three residues in AATB3 The loss of the activity from the mutations together with the multiple alignment analysis indicated that the Asp232 residue
of AATB3 enhances the function of the enzyme-bound coenzyme PLP and that the Lys270 residue mediates binding of PLP, whereas the Arg403 residue is respon-sible for recognizing the a-COO) of the substrate These functions are performed by the corresponding residues of Asp222, Lys258 and Arg386 of the AAT from E coli [15,16,19,20]
We also described in detail the physicochemical and catalytic properties of AAT from B subtilis B3 The purified enzyme was demonstrated to have an optimal temperature at 45C and thermostability of only up to
Fig 5 Characterization of the purified AATB3 (A) Effect of
tempera-ture on activity of AATB3 (pH 7.2) (B) Thermostability of AATB3 The
enzyme was pre-incubated at 40, 50, 60 or 65 C for 5, 15 or 30 min
before the assay (C) Effect of pH on activity of AATB3 The assay
was performed at 45 C in buffers with pH in the range 4.4–10.2.
Table 2 Effect of metal ions on the activity of purified AATB3 Values represent the means of triplicates relative to the untreated control samples.
Chemicals
Relative activity (%)
Table 3 Kinetic parameters for recombinant AATB3 from Bacil-lus subtilis B3 Kinetic parameters were obtained from double reci-procal plots as described in the Materials and methods Values represent the mean ± SD of three determinations.
Substrates
B subtilis B3 AATB3
V max
(m M ÆL)1Æmin)
k cat
(s)1) K m (m M )
k cat ⁄ K m
(m M )1Æs)1)
L -aspartate 0.23 ± 0.03 30 ± 3 6.68 ± 1.45 4.50
L -glutamate 0.07 ± 0.01 14 ± 2 8.00 ± 1.32 1.75 a-ketoglutarate 0.21 ± 0.01 27 ± 1 0.32 ± 0.08 84.38 Oxaloacetate 0.11 ± 0.01 22 ± 1 0.60 ± 0.06 36.67
Trang 850C These characteristics are similar to those of the
AAT from E coli [30] and not of AATs from the Ib
subgroup, which usually have high thermostability The
thermostability appears to be related to the amino acid
composition of the AAT Okamoto et al [6] reported
that the high Pro content of the Ib-type AAT from
T thermophilus (6.5%) will render the enzyme rigid
and thermostable The same features are also found in
other subgroup Ib AATs, such as Thermus aquaticus
YT1 AAT (7.0%) [31] and Phormidium lapdideum
(6.1%), as well as the newly found Ib-prokaryote-type
AAT in Pinus pinaster (6.4%) [9,11] The Pro content
of B subtilis B3 AAT is 4.1%, which is similar to that
of subgroup Ia E coli AAT (3.8%) and is much lower
than that of subgroup Ib T thermophilus AAT For
this reason, the thermostability of B subtilis B3 AAT is
similar to that of E coli AAT and is lower than that of
T thermophilusAAT by 20 C [6]
We showed that the AAT from B subtilis B3 had an
optimal pH at 8.0 and had relatively high activity over
a wide alkaline pH range (pH 7.0–9.0) This
character-istic is similar to that of the AAT from B circulans
The B circulans AAT has been reported to have high
optimal pH and a wide pH stability range as a result
of the N-terminal two a-helical segments, which
con-tain an additional sequence of 32 acid residues not
found in many AATs [23] Interestingly, B subtilis B3
AAT also has a similar additional N-terminal sequence
of 32 acid residues (Fig 1), which shows 53% identity
with that of B circulans AAT, and the additional
N-terminus of B subtilis B3 AAT appears to perform
the same function as that of B circulans AAT
The results obtained in the present study indicate
that the AAT from B subtilis B3 can catalyze
l-aspar-tate, l-glutamate, l-tryptophan, l-tyrosine and
l-phen-ylalanine transamination, with l-aspartate being the
best substrate However, the activity of AATB3
toward three aromatic amino acids were weak, similar
to that of AAT from Bacillus sp YM-2 strain [32],
and was unlike AAT from E coli, which was shown to
have 22% of the activity of the total tyrosine
amino-transferase [33] The Km values for AATB3 were 6.7,
0.3, 8.0 and 0.6 mm for l-aspartate, a-ketoglutarate,
l-glutamate and oxaloacetate, respectively Similar to
the other AATs, the Kmvalues for oxo acids are lower
than that for the amino acids [9,32,34] However, it is
worth noting that both kcat and kcat⁄ Km values are
lower than those of AAT from E coli [35]
This new AAT phylogenetically belongs to subgroup
Ib of AAT, although it also has conserved active
resi-dues and thermostability characteristic of Ia-type
AATs Although our combined results appear to be
contradictory, we propose that the B subtilis gene
described in the present study may have arisen from the interaction between the Ia-type and Ib-type aat genes during evolution A similar phenomenon is seen when the genome segment of B subtilis B3 is com-pared with those of B subtilis A1⁄ 3 and B amylolique-faciensFZB42 The aatB3 gene frequently appears in the region between the srf operon and sfp gene This region is the putative regulatory region relevant to bio-synthesis of the lipopeptides, especially for the sfp gene, which is essential for biosynthesis of the lipopep-tides [26] We presume that the aat gene in this region can regulate the biosynthesis of the lipopeptides The experiments performed in the present study showed that this AAT can form Glu and Asp, and the forma-tion of Glu and Asp is used to synthesize Gln and Asn, respectively These four residues are common components in lipopeptides, such as surfactin, iturin and fengycin Another interesting observation was that the B subtilis B3 has another aat gene similar to aspB outside this region This could be explained by the need to synthesize more AATs to provide adequate nutrients (carbon and nitrogen sources) and lipopep-tides so as to survive in complex environments and deal with competitors
In summary, a new AAT with an additional N-ter-minal sequence was identified from B subtilis B3 Having both Ia-type and Ib-type characteristics and a high activity over an alkaline pH range, this enzyme may regulate the biosynthesis of lipopeptides and has various potential industrial applications, such as
in the synthesis of l-tyrosine, l-phenylalanine and
l-homophenylalanine A detailed characterization of the role of B subtilis B3 AAT and its structure are in progress
Materials and methods
Bacterial strains, plasmids and growth conditions
The bacterial strains and plasmids used in the present study are described in Table 4 E coli DH5a was used as the host for amplification of all plasmids, and recombinant proteins were expressed in E coli BL21 B subtilis B3 was used for cloning the aatB3 gene LB broth was used for the growth
of E coli and B subtilis strains When required, antibiotics were added at the final concentrations: ampicillin (Amp),
100 lgÆmL)1; kanamycin (Km), 50 lgÆmL)1
DNA manipulation and transformation
The isolation and manipulation of recombinant DNA were performed using standard techniques All enzymes used in the present study were purchased from Takara Bio Inc
Trang 9(Otsu, Japan) The specific primers used for the PCR are
described in Table 5
The original sequence of the aatB3 gene was obtained
through the B subtilis B3 gene library constructed in a
pre-vious study (accession number AY040867) [25] To express
the recombinant AATB3 protein in E coli, the entire aatB3
ORF was amplified using primers P1 and P2 using B
sub-tilisB3 chromosomal DNA as the template; the amplified
product was digested with KpnI and EcoRI, and cloned into
the same sites of the cloning vector pUC19 and expression
vector pET30a(+), resulting in the plasmids pUCAAT and
pETAAT, respectively The entire cloned regions were
con-firmed by sequencing (Invitrogen Biotechnology Co., Ltd, Shanghai, China)
Site-directed mutagenesis via PCR
Single mutations were introduced into the cloned AATB3 using the Takara MutanBEST Kit (Takara) Reactions were carried out using the primer pairs: for D232N, D232N-F and D232N-R; for K270H, K270H-F and K270H-R; and, for R403Y, R403Y-F and R403Y-R The pUCAAT vector was used as a template The introduced mutations in the aatB3 gene were confirmed by DNA sequencing The resulting
pUCR403Y, and the three different DNA fragments carrying mutant aatB3 genes from these vectors were subcloned into the KpnI and EcoRI restriction sites of the pET30a(+) expression vector to obtain pETD232N, pETK270H and pETR403Y, respectively
Expression and purification of recombinant wild-type and mutant AATB3 enzymes
The E coli strain BL21 (DE3) was transformed with pETAAT or the three expression plasmids carrying different
Table 4 Bacterial strains and plasmids used in the present study Resistance markers were: Amp r , ampicillin resistance; Km r , kanamycin resistance.
Strains
E coli
B subtilis
Plasmids
Darmstadt, Germany)
pETAAT The aatB3 fragment was inserted into KpnI and EcoRI sites of
pET30a(+) for the expression of protein AATB3; T7 promoter-based expression vector; Km r
Present study
pUCAAT The aatB3 fragment was inserted into KpnI and EcoRI sites of
pUC19 for construction the mutant of AATB3 protein; Ampr
Present study
pETD232N The fragment from pUCD232N was inserted into KpnI and EcoRI
sites of pET30a(+) for the expression of protein D232N; T7 promoter-based expression vector; Km r
Present study
pETK270H The fragment from pUCK270H was inserted into KpnI and EcoRI
sites of pET30a(+) for the expression of protein K270H; T7 promoter-based expression vector; Km r
Present study
pETR403Y The fragment from pUCR403Y was inserted into KpnI and EcoRI
sites of pET30a(+) for the expression of protein R403Y; T7 promoter-based expression vector; Km r
Present study
a Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing, China.
Table 5 Oligo DNA primers used in the present study Restriction
sites or mutation sites in primers are underlined.
Trang 10mutant aatB3 genes The transformants were cultivated at
37C with shaking in LB medium containing 50 lgÆmL)1
kanamycin until D600 of 0.5–0.7 was reached Flasks
con-taining the cultures were supplemented with isopropyl
thio-b-d-galactoside at a final concentration of 1 mm After
incu-bation at 37C for a further 6 h with vigorous shaking, the
cells were harvested by centrifugation at 6000 g for 20 min
The cell pellets were resuspended in a buffer containing
20 mm potassium phosphate, 500 mm NaCl, 5% glycerol
and 20 mm imidazole buffer at pH 7.3 Cells were lysed by
sonication, and cell debris was removed by centrifugation at
10 000 g for 20 min The recombinant enzymes were purified
by a single chromatographic step using HisTrapHP (GE
Healthcare, Milwaukee, WI, USA) The column was loaded
with the bacterial cell lysate, and the non-adherent proteins
were removed by rinsing with 20 volumes of wash buffer
(20 mm potassium phosphate, pH 7.3, 5% glycerol, 500 mm
NaCl, 20 mm imidazole) The proteins were eluted with a
gradient of 10–500 mm imidazole in wash buffer The
purified enzymes were stored at )20 C after salt removal
using the HiTrap Desalting columns (GE Healthcare)
Pro-tein concentrations were measured with a BCA-100 proPro-tein
quantitative analysis kit (Biocolor Biotech, Shanghai, China)
using BSA as the standard
Determination of enzyme activities
AAT activity was assayed as described by Collier and
Kohlhaw [36] The assay mixture contained (in 0.8 mL total
volume): 0.1 m potassium phosphate buffer (pH 7.2),
30 mm l-aspartate, 10 mm a-ketoglutarate, 38 lm pyridoxal
5¢-phosphate and enzyme The stock solution of
a-ketoglu-tarate was prepared daily, and its pH was adjusted to 7.2
with NaOH The assay was performed at 25C for 20–
40 min, and the reaction was stopped with 0.1 mL of 10 m
NaOH After 30 min at room temperature, the increase in
absorbance at 265 nm was measured for the test sample, as
well as a control to which NaOH had been added before
the addition of a-ketoglutarate A molar extinction
coeffi-cient for oxaloacetate of 780 m)1Æcm)1 was used, and one
unit of activity was defined as the amount of enzyme
neces-sary to form 1 lmolÆmin)1of oxaloacetate
The aromatic amino acid aminotransferases were assayed
according to Mavrides and Orr [37] The assay was
estab-lished for AAT except that aspartate was replaced with
6 mm tryptophan, tyrosine or phenylalanine, and the
con-centration of the a-ketoglutarate was decreased to 10 mm
The increase in absorbance of the reaction solution was
measured at 335, 330 and 315 nm The molar extinction
coefficients for the reaction products indole pyruvate,
q-hy-droxyphenylpyruvate and phenylpyruvate were 10 000,
19 500 and 17 500 m)1Æcm)1, respectively One unit of
aro-matic amino acid aminotransferase activity was defined as
the amount of enzyme necessary to form 1 lmol of indole
pyruvate, q-hydroxyphenylpyruvate or phenylpyruvate
A paper chromatography assay for amino acids was also used to detect the activity toward tryptophan The reaction was performed as described above, and a-ketoglutarate and oxaloacetate were used as amino acceptors At the end of the reaction, 10 lL of the reaction solution was spotted onto a filter paper and separated by chromatography (n-butyl alcohol⁄ ethanol ⁄ water at 4 : 1 : 1, v ⁄ v) Subse-quently, the filter paper was sprayed with 0.1% ninhydrin After drying, the products of the amino acid on the filter paper were displayed purple in color
To determine the effects of pH, temperature and inhibi-tors, l-aspartate and a-ketoglutarate were used as amino donor and acceptor, respectively, and the reactions were performed as described above To investigate the effect of
pH at the optimum temperature (45C), three buffered systems at a final concentration of 50 mm were used: acetate⁄ sodium acetate (pH 4.4–6.0), potassium phosphate (pH 6.0–8.0) and glycine⁄ sodium hydroxide (pH 8.0–10.2) The temperature dependence was determined at pH 7.2, and the stability of the enzyme was examined by keeping the pure preparation for 5, 15 and 30 min at 40, 50, 60 and 65C before the assay The effect of inhibitors was established with the reaction system containing different metal ions at final concentrations of 1 and 10 mm The specific activities for amino acids were analyzed under similar conditions
Kinetic experiments
For determination of kinetic parameters, an assay was established by coupling with malate dehydrogenase as described previously [38] In the routine assay, the reaction
(pH 7.6), 25 lm pyridoxal 5¢-phosphate, 0.5 mm NADH, 0.08 U malate dehydrogenase and 0.5 lL of purified enzyme in a reaction volume of 200 lL The temperature was 30C The reaction was monitored by the decrease in absorbance of NADH at 340 nm over 180 s with a Thermo Multiskan Ascent (Thermo Fisher Scientific Inc., Waltham,
MA, USA) and the data were recorded every 20 s AAT substrate concentrations were varied in the range 1–20 mm
l-aspartate with a fixed concentration of 10 mm a-ketoglu-tarate (for KL asp
m ) and in the range 0.5–10 mm a-ketogluta-rate with a fixed concentration of 20 mm l-aspartate (for
Ka KG
m ) The kinetic parameters for l-glutamate and oxalo-acetate were coupled to glutamate dehydrogenase [39] Our assay was established using the same methods, and the
200 lL reactions contained l-glutamate, oxaloacetate,
1 mm NADH, 2 U of glutamate dehydrogenase and 12 mm
NH4Cl (as second substrate for glutamate dehydrogenase)
in 0.1 m potassium phosphate buffer (pH 7.6) AAT sub-strate concentrations were varied in the range 1.0–27 mm
l-glutamate with a fixed concentration of 5 mm oxaloace-tate (for KL glu
m ) and in the range 0.5–20 mm oxaloacetate
(for KOAA
m ) Km and Vmax values were estimated from the