Genetic variation in the VP7 gene of rot

1998–2009 Nguyen Van Tranga, Tetsu Yamashirob,c, Le Thi Kim Anha, Vu Thi Bich Haua, Le Thi Luand, Dang Duc Anha,∗ aThe National Institute of Hygiene and Epidemiology, Ha Noi, Viet Nam bC

1998–2009

Nguyen Van Tranga, Tetsu Yamashirob,c, Le Thi Kim Anha, Vu Thi Bich Haua, Le Thi Luand,

Dang Duc Anha,∗

aThe National Institute of Hygiene and Epidemiology, Ha Noi, Viet Nam

bCenter for Infectious Disease Research in Asia and Africa, Institute of Tropical Medicine, Nagasaki University, Japan

cVietnam Research Station, Japan Initiative for Global Research Network on Infectious Diseases (J-GRID), Ha Noi, Viet Nam

dCenter for Research and Production of Vaccines and Biologicals, Ha Noi, Viet Nam

a r t i c l e i n f o

Article history:

Available online 25 February 2012

Keywords:

Rotavirus

G1P[8]

VP7

Vietnam

a b s t r a c t

GroupArotavirusgenotypeG1P[8]isthemostcommonstrainaffectinghumansaroundtheworldover thepastfewdecades.Inthisstudy,weexaminedgeneticvariationintheVP7geneofrotavirusG1P[8] strains,detectedinchildrenoffourmajorcitiesofVietnamduringthreedifferentrotavirusseasons: 1998–1999,2007–2008and2008–2009inordertoassesstheevolutionofthevirusover11years.Fecal samples(n=73)fromchildrenhospitalizedforgastroenteritiscausedbyG1P[8]rotaviruswereanalyzed

byDNAsequencingofgene9encodingtheVP7capsidprotein.PhylogeneticanalysesindicatedthatVP7 geneoftheG1strainsfrom1999containedalineageI,whilerotavirusesfrom2009clusteredinlineage

II.Bothoftheselineageswerefoundco-circulatingin2007–2008season.Whiledifferentsublineages

oflineageIandIIco-circulatedinthe1998–1999and2007–2008seasons,almostallstrainsin2009 belongedtosub-lineageII-C.Intheanalysisusingselected10strains,theVP4genesofthese2VP7-G1 lineageswereallgroupedinF45-likecluster.Deducedaminoacidanalysesindicatedthattherewere thirteenaminoacidsubstitutionsbetweenstrainsoftwolineages.Ofthose,twowerefoundinantigenic regionsAandC,implyingpossibleantigenicdifferencesbetweenthesetwolineages.TheG1P[8]strains

inVietnamareverygeneticallydiverseanddynamic,implyingthefrequentmonitoringonevolutionof rotaviruswillbeimportanttoassessefficacyofrotavirusvaccineinVietnam

© 2012 Elsevier B.V All rights reserved

E-mail address:ducanhnihe@hn.vnn.vn (D.D Anh).

The total numbers of G1P[8] strains detected in 2007–2008 and 2008–2009

(Hoshinoetal.,2004).Inthisstudy,weexaminedmutationsingene

2.1 Samples

2.2 Sequencing of VP7 and VP4 regions

(Gentschetal.,1992).Thecomparisonanalysiswasconductedon

relation-ship(Tamuraetal.,2007)amongsequencesstudied.Themethod

JN790709–JN790718

EF672581

AB118025

3.1 Analyses of VP7 genes from G1P[8] strains collected during

IA

ID

IC

IE

IIC

IIB IID IIA IIE

Fig 2 Phylogenetic tree obtained from nucleotide sequence analyses on VP4 genes of P[8] rotavirus isolated in Vietnam (in boxes) Reference strains were selected from

(Table1)

2009;Matthijnssensetal.,2010b;Richardsonetal.,1984)

3.2 Deduced amino acid differences between the VP7 proteins of lineage I and lineage II strains of G1 rotavirus

Fig 3 Amino acid substitutions in the VP7 protein that are observed between lineages I and II Antigenic regions (A, B, C) are indicated Selected strains from different cities

(JayasingheandPalombo,1999).AstudybyTrinhetal.showedthat

sublin-eageIBandIIB(Aroraetal.,2009).Thereforeitissuggestedthat

vaccine

None

Acknowledgments

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