Antioxidant activity of the flavonoid hesperidin in chemical and biological systems

Antioxidant Activity of the Flavonoid Hesperidin in Chemical and Biological Systems Antioxidant Activity of the Flavonoid Hesperidin in Chemical and Biological Systems Scientific seminar Lam Thu Huyen K63D2 Phamarceutical Chemistry Ha Noi university of Science I Background I Background General about hesperidin Wilmsen P ; J Agric Food Chem 2005, 53, 4757−4761 Hesperidin I Background I Background properties of hesperidin Wilmsen P ; J Agric Food Chem 2005, 53, 4757−4761 antiallergenic anticarcino.

Antioxidant Activity of the Flavonoid Hesperidin in Chemical and Biological

Systems

Scientific seminar - Lam Thu Huyen

I.Background: General about hesperidin

Hesperidin

I.Background: properties of hesperidin

antimicrobial vasodilator properties

I.Background: hesperidin's ability to scavenge free radicals and reactive oxygen species

cancer cell

1 General about free radicals

I.Background: hesperidin's ability to scavenge free radicals and reactive oxygen species

 orange juice

 mandarin juice

→ reduce in colon and lung cancer

2 Experiment

and result:

→ study the antioxidant activity of hesperidin

I.Background: method used to evaluate antioxidant capacity of hesperidin

Method: in vitro

In vivo

hesperidin & trolox

(diluted in DMSO)

250 µM of DPPH (radical) dissolved in ethanol + Tris-HCl buffer ( difference

concentration)

maintained in dark 20min befor using spectrophometry method

at 517 nm

III.Methods & results: modified Yamaguchi et al method

III.Methods & results: modified

Yamaguchi et al method

 reduced the level of the DPPH٠ in a similar way to the

antioxidant trolox ( positive control) except 10 µM

 higher capacity of reducing the DPPH radical than trolox

Figure 2 DPPH٠ concentration reduced by hesperidin (■) and trolox ()

III Methods & results: using yeast cells

. Evaluation of antioxidant capacity using yeast cells

2 Advantages of method

. testing of various products

. fast

. sensitive

. reproducible

. reliable results

3 Yeast use in method

Saccharomyces cereVisiae

• possesses a variety of antioxidant defenses

• in experiment: using superoxide-dismutase ( SOD) proficient and deficient strains

III Methods & results: using yeast cells

3 Procedure

hesperidin in DMSO

( 25 and 50 µM)

stressing agents 1mM (paraquat herbicide

& hydrogen peroxide)

2 × 106 cells/mL exponential-phase S.cereVisiae cells

III Methods & results: using yeast cells

incubated in YPD medium

6h, 28°C, 130 rpm

diluted in 0.9% (w/v) NaCl, plated onto YPD agar

incubated 72h, 28°C

compare with control plates (beginning)

III Methods & results: using yeast cells

4 Result

Table 2 Survival of SOD Proficient and Deficient S cerevisiae Strains Treated and Untreated with the Antioxidant Hesperidin and/or the

Stressing Agents Paraquat or Hydrogen Peroxide

• protect cells

from damage

induced by

stressing agents

IV Conclusions & discussions