A smaller population that is readily accessed by agonist peptides on the surface of intact cells constitutes less than 30% of Y2 receptors detected in particulates after cell homogenizat
Trang 1P R I O R I T Y P A P E R
A pool of Y2 neuropeptide Y receptors activated by modifiers
of membrane sulfhydryl or cholesterol balance
Steven L Parker1, Michael S Parker2, Justin K Kane1and Magnus M Berglund3
1
Department of Pharmacology, University of Tennessee College of Medicine, Memphis, TN, USA;2Department of Microbiology and Molecular Cell Sciences, University of Memphis, TN, USA; 3 Unit of Pharmacology, Department of Neuroscience,
University of Uppsala, Sweden
The cloned guinea-pig Y2 neuropeptide Y (NPY) receptors
expressed in Chinese hamster ovary (CHO) cells, as well as
the Y2 receptors natively expressed in rat forebrain, are
distributed in two populations A smaller population that is
readily accessed by agonist peptides on the surface of intact
cells constitutes less than 30% of Y2 receptors detected in
particulates after cell homogenization A much larger
frac-tion of cell surface Y2 sites can be activated by sulfhydryl
modifiers A fast and large activation of these masked or
cryptic sites could be obtained with membrane-permeating,
vicinal cysteine-bridging arsenical phenylarsine oxide A
lower activation is effected by N-ethylmaleimide, an
alkyla-tor that slowly penetrates lipid bilayers The restricted-access
alkylator, 2-[(trimethylammonium)ethyl]methanethiosulfo-nate, was not effective in unmasking these sites Some of the hidden cell surface Y2 sites could be activated by polyene filipin III through complexing of membrane cholesterol The results are consistent with the presence of a large Y2 reserve
in a compartment that can be accessed by alteration of sulfhydryl balance or fluidity of the cell membrane, and
by treatments that affect the anchoring and aggregation of membrane proteins
Keywords: receptor sequestration; receptor reserve; receptor signaling; receptor masking
Synaptic discharge of many neurotransmitters produces
concentrations of these receptor agonists that saturate the
respective binding sites, with a potential for prolonged and
excessive signaling With receptors characterized by high
binding affinities, which represent a large fraction of
rhodopsin-related neurotransmitter receptors, it may not
be possible to adequately constrain the signaling by
dissociation of the agonist For neuropeptide receptors, a
paracrine regulation via secretion of specific peptidases
would meet large difficulties in both the selectivity and the
economy of action Scavenging by cell membrane- resident
ectoproteinases by way of in situ encounters with
extra-cellular agonists may not satisfy the clearance needs
created by agonist discharge A much more selective (and
potentially quicker) regulation could be provided by
sequestration or internalization of the receptor–ligand
complex and further intramembrane or intracellular
processing (reviewed in [1,2]) This could be accomplished
by recycling sequestration (e.g the m1 muscarinic receptor [3]), by recycling internalization (e.g the m2 muscarinic receptor [4] or the neuropeptide Y (NPY) Y1 receptor [5]), and by lysosome-linked disposing internalization (e.g the endothelin-B receptor [6]), all possibly enacted in relation
to the prevailing levels of the respective agonists and the extent of preservation of the respective receptor molecules Among neuropeptide transmitters, large levels of NPY are present in many areas of the forebrain [7], enabling an important regulation of feeding [8] The forebrain NPY receptors include all principal Y receptor types [9], with Y1 and Y2 receptors detected at largest levels [10] The slowly internalizing Y5 receptors [11] could represent a substantial component of sustained feeding regulation by NPY However, both the Y5 and the feeding-coregulative [8] Y1 receptors (which could be strongly driven to internalize even by picomolar concentrations of NPY [5,12]) might be overwhelmed by large NPY release, in view of high nanomolar levels of the peptide in rodent [7] and even in human forebrain locations [13] The discharge overloads could be handled through participation of another NPY receptor, the Y2 receptor The Y2 receptor
is strongly expressed especially in hypothalamic areas [10], and exists in two affinity states, one of which shows a very high binding affinity and is linked to a large degree of receptor aggregation [14] The Y2 receptor is also distinguished by a low rate of internalization compared
to the Y1 receptor when expressed in CHO cells [12] A large portion of the Y2 complement is not detected on membranes of intact cells, but becomes accessible to agonist peptides upon cell homogenization, or upon treatment with a membrane-penetrating crosslinker of
Correspondence to S L Parker, Department of Pharmacology,
University of Tennessee College of Medicine, Memphis,
TN 38163, USA.
Tel.: + 1 901 850 7617,
E-mail: StevenLeonardParker@msn.com
Abbreviations: NPY, neuropeptide Y; hNPY, human/rat NPY;
hPYY(3–36), human peptide YY(3–36); PYY, peptide YY; NEM,
N-ethylmaleimide; MTSET,
2-[(trimethylammonium)ethyl]methane-thiosulfonate bromide; PAO, phenylarsine oxide.
(Received 22 February 2002, revised 18 March 2002,
accepted 22 March 2002)
Trang 2vicinal cysteines, phenylarsine oxide (PAO) [12] This
study presents evidence for activation of these sites by
agents that affect the membrane sulfhydryl balance or
cholesterol, and also the rate of internalization of the Y2
receptor
M A T E R I A L S A N D M E T H O D S
Chemicals
The Y peptides hPYY(3–36) and hNPY were obtained from
the American Peptide Company (San Diego, CA, USA)
Filipin III, N-ethylmaleimide (NEM) and phenylarsine
oxide were purchased from Sigma (St Louis, MO, USA)
Cholesteryl hemisuccinate was obtained from Calbiochem
(La Jolla, CA, USA) Filipin III and PAO were dissolved in
dimethylsulfoxide and stored in aliquots at)80 C Filipin
complex (Sigma; a mixture of three isomers of filipin) was
only about 25% as active as filipin III, and hence was not
used Cholesteryl hemisuccinate was prepared as a water
emulsion and also stored at )80 C Restricted-access
methanethiosulfonate MTSET
(2-[(trimethylammo-nium)ethyl]methanethiosulfonate bromide) was obtained
from Toronto Research Chemicals (North York, Ontario,
Canada) Solutions of this agent and of NEM were made
within 15 min before use
Labeled peptides
All iodinations of Y peptides were performed as described
previously [15] The radioactive Y peptides were 75–90%
monoiodinated and had specific activities in the range of
1500–1800 CiÆmmol)1(70–80% theoretical), as deduced by
comparison in saturation assays with HPLC-purified
monoiodinated 125I-labeled Y peptides hNPY and
hPYY(3–36) supplied by PerkinElmer/NEN, Cambridge,
MA, USA (specific activity 2170 CiÆmmol)1)
Cell cultures and labeling
All cell types were cultured in F12/D-MEM medium (Gibco,
Long Island, NY, USA) at 250 lgÆmL)1of geneticin and
2 mM GlutaMax1 (Gibco) The guinea-pig Y1 receptor
(gpY1-CHO; [16]); and the guinea-pig Y2 receptor
(gpY2-CHO; [17]) were expressed in Chinese hamster ovary (CHO)
cells The cell lines used in this study had stable particulate
receptor density, at the level of 10–20 fmol per 100 000 cells,
over up to 40 passages in F12/D-MEM at 250 lgÆmL)1of
geneticin (Gibco) At full confluence, which was reached
within 48 h with seeds of 25 000 cells per cm2 at 37C
in 95% O2/5% CO2, the cell count was 200 000–235 000
per cm2 After four washes with OptiMem medium
(Gibco, Long Island, NY, USA) to remove any external
proteinase activity, the experimental incubations were
car-ried in 48-well (0.8 cm2Æwell)1) plates, in a volume of
0.25 mL, using OptiMem medium The labeling of Y
peptides was carried out at 50 pM of 125I-labeled peptide
tyrosine, using 300 nMnonlabeled peptides for
nonsaturat-ing (or nonspecific) bindnonsaturat-ing correction In all experiments,
less than 15% of the labeled peptides were degraded over the
incubation period, as verified by Bio-Gel P-4
chromatogra-phy [15] The incubations were terminated by the removal of
the medium by suction, two washes with cold Opti-Mem
buffer, and extraction for 12 min at 0–4Cwith ice-cold 0.2MCH3COOH/0.5MNaCl (pH 2.6), which was found to quantitatively dissociate the cell-surface attached Y peptides, without significant extraction of internalized peptides [12,18] The binding of Y peptides to particulates from gpY2-CHO cell or rat forebrain tissue particulates was, on the other hand, more than 90% extracted by cold acid saline,
as expected from the known importance of the arginine residues of NPY in Y2 (and Y1) receptor binding [19] Rat forebrain tissue (a pool of hypothalamic and piriform cortex slices) was diced by scissors into fragments 1-mm
in diameter, and then dispersed in 0.14MNaCl/0.01MNa phosphate/0.001M EDTA (NaCl/Pi/EDTA buffer; final
pH 7.4) at about 50 mg fresh tissue per ml, using five slow passages through a 12-gauge needle The suspension was brought to 5 FALGPA units per mL (5 lmol of furyl-acroyl-Leu-Gly-Pro-Ala hydrolyzed per min at pH 7.5 and
25C) of Sigma collagenase and incubated for 60 min at 23–24Cwith five slow passages through 16-gauge and 18-gauge needles repeated at 10-min intervals The suspension was then filtered through a 100-mesh sieve, and the filtrate sedimented for 5 min at 100 g to recover the cells The cells (>95% excluding Trypan blue stain) were resuspended in Opti-Mem buffer, and 200 000 cells per well were imme-diately plated for experimental incubations The density of Y2 receptors did not significantly change in the above dissociation procedure and the additional incubation of up
to 2 h at 37C At the end of the incubation period, the cells were brought to 0–4C, harvested by sedimentation at
100 g for 5 min and surface-washed with cold Opti-Mem buffer The pellets were then dispersed in ice-cold 0.2M
CH3COOH/0.5M NaCl, and after 12 min at 0–4C sedimented for 5 min at 4000 g to separate the extracted (originally cell-surface attached) and the residual (internal-ized) radioactive peptide
Receptor characterization The homogenization or NPY receptor assay buffer con-tained 8% sucrose, 0.2% proteinase-free BSA (Sigma), 0.025% bacitracin, 1 mM diisopropylfluorophosphate (Sigma), 4 mMCaCl2, 2 mMMgCl2, 20 mM hepes.NaOH (pH 7.4) and 50 lM ATP Particulates from gpY2-CHO cells or from dispersed rat forebrain cells were isolated by homogenization in the cold NPY receptor assay buffer, applying 12 complete strokes of a Teflon pestle (clearance 0.10 mm) in a Potter-Elvehjem homogenizer at about
800 r.p.m., followed by the removal of debris for 5 min at
100 g, and the sedimentation of particulates for 15 min at
16 000 g (all at 0–4C) The pelleted particulates were aliquoted and kept at )80 C Particulate receptors were assayed as described previously [20] The particle concen-tration was 100–125 lgÆmL)1, the assay volume was 0.2 mL, and the incubation time was 90 min at 23–24C, with the appropriate competitors or inhibitors The assay was terminated by centrifugation for 15 min at 12 000 g at
4C, the supernatants were discarded, and the pellets surface-washed by cold assay buffer prior to counting in a gamma-scintillation counter The binding properties of the cell-surface receptors were characterized on monolayer cultures in Opti-Mem medium Iodinated Y peptides were input at 50 pM, and competed by up to nine concentrations
of homologous or isologous peptides in the range of
Trang 33· 10)11to 1· 10)6M Polyethyleneglycol precipitation of
particulates was carried out as described previously [15]
Data evaluation
Binding parameter calculations were carried out in the
LIGAND program [21] Multiple comparisons following a
positiveANOVAwere done in Tukey’s t-test [22]
R E S U L T S
Activation of cell surface Y2 sites by phenylarsine oxide
PAO inhibited the binding of Y2-selective agonist hPYY
(3–36) to particulates from gpY2-expressing cells or rat
forebrain only at concentrations above 1 mM (Table 1)
Pretreatment with the arsenical at 100 lM decreased the
affinity of the Y2 binding to CHO cell or forebrain
particulates by not more than 40% (Table 2) However, PAO consistently increased the Y2 agonist binding to CHO cell monolayers by fourfold to fivefold (Fig 1A), as previously shown [12] This activation occurred with little change in Y2 site affinity (Fig 1A) The binding of the Y2 agonist to dispersed rat forebrain cells was also increased by PAO up to fourfold (Fig 1B), without a significant change
in affinity (Fig 1B) The activation by PAO saturated with increasing concentration of the arsenical between 10 and
30 lMwith either the Y2-CHO cells (Fig 1A), or with rat forebrain cells (Fig 1B) Pretreatment of gpY1-CHO cells
at 24 or 37Cwith 30 lMPAO did not produce significant change in subsequent surface labeling by125I-labeled hNPY
or125I-labeled (Leu31,Pro34)hPYY (data not shown) The activation of Y2 sites on intact cells by PAO did not involve more than half of the receptors detectable in particulates after cell homogenization (Fig 1A) Homoge-nization of gpY2-CHO cells alone resulted in a large activation of Y2 sites, to about twice the level measured after treatment of cell monolayers by PAO, and exceeding the monolayer control binding by a factor of at least four in the absence of PAO treatment Pretreatment or cotreatment
of either the cell monolayers or the isolated particulates by PAO (at 30 lM) did not produce a consistent increase in particulate Y2 binding relative to control preparations (Fig 1A) Likewise, the Y2 binding to particulates isolated from homogenates of dispersed rat forebrain cells was increased more than sixfold relative to whole cells, but not
Table 1 Inhibition of Y2 receptor binding by the agents used
Particu-lates from gpY2-CHO cells or rat piriform cortex were labeled over
90 min at 23–24 Cby 50 p M125I-labeled hPYY(3–36) in the presence
of 10–12 different concentrations of the respective agents, between 0.01
and 10 m M The results are averages of three separate experiments,
shown ± SEM The assay conditions are specified in the Methods
section The nonspecific binding was defined at 300 n M of nonlabeled
hPYY(3–36) Percent of the total specific binding displaced at 10 m M
of an agent is shown in parenthesis after the corresponding K I value.
K I , m M
Agent guinea pig Y2-CHO rat forebrain
Dithiothreitol 3.37 ± 0.66 (46%) 3.35 ± 0.44 (34%)
Phenylarsine oxide 2.24 ± 0.75 (78%) 1.51 ± 0.34 (94%)
N-Ethylmaleimide 0.146 ± 0.014 (75%) 0.708 ± 0.104 (73%)
MTSET 0.264 ± 0.027 (72%) 1.86 ± 0.14 (65%)
Filipin III > 0.1 > 0.1
Table 2 Effect of pretreatment with various sulfhydryl-active agents on
the affinity of Y2 binding The K d values are in p M hPYY(3–36),
± SEM The particulates were preincubated for 30 min at 24 Cin the
assay buffer in presence of the indicated molarities of SH-active agents,
then sedimented to remove the agents, surface-washed and assayed (see
Materials and methods) Data represent triplicate competition assays
employing 8–10 concentration points in the range of 10–30 000 p M
nonlabeled Y2 agonist, and 50 p M125I-labeled hPYY(3–36) Defining
the nonspecific binding at 100 n M nonlabeled hPYY(3–36), more than
90% displacement of 125 I-labeled hPYY(3–36) was observed at 30 n M
unlabeled hPYY(3–36) after any treatment Note that the Y2 binding
in both C HO and rat forebrain cells could be resolved into two
sig-nificant components with K d values of 5–15 and 300–700 p M ,
respectively [14].
K d , p M
Agent and molarity used gpY2-CHO cells Rat forebrain cells
Control 422 ± 40 316 ± 43
Dithiothreitol (1 m M ) 541 ± 70
Phenylarsine oxide (100 l M ) 627 ± 70 334 ± 52
N-Ethylmaleimide (30 l M ) 601 ± 72
MTSET (100 l M ) 637 ± 46
Fig 1 Effects of phenylarsine oxide (PAO) on the binding of Y2 agonist
125 I-labeled hPYY (3–36) to gpY2-CHO monolayers or particulates, and
to dispersed rat forebrain cells The labeled Y2 agonist was input at
50 p M in all cases For assay details see Methods All data, shown
± SEM, are averages of three experiments Asterisks indicate differ-ences significant vs the control binding in the Tukey t-test at the level
of 95% (*) or 99% (**) confidence (A) The surface binding to monolayers or particulates from gpY2-CHO cells as related to pre-treatment (30 min at 37 C) of monolayers with PAO in the range of 3–100 l M , and pretreatment or cotreatment of particulates with 30 l M
of the arsenical Competition by nonlabeled hPYY(3–36) showed K d
of 442 ± 37 p M for control surface binding, and of 638 ± 74 p M for surface binding after pretreatment by 30 l M PAO (n ¼ 3) (B) The surface binding to dispersed rat forebrain cells after pretreatment (30 min at 37 C) with 3–100 l M PAO, and also the binding to par-ticulates from cells pretreated for 30 min at 37 Cwith 30 l M PAO Competition by nonlabeled hPYY(3–36) showed K d of 407 ± 38 p M
for control surface binding, and of 451 ± 24 p M for surface binding after pretreatment by 30 l PAO (n ¼ 3).
Trang 4further augmented significantly by a pretreatment with
30 lMPAO (Fig 1B)
The effect of 30 lMPAO could be largely suppressed by
100 lM sulfhydryl protector dithiothreitol, and was
com-pletely prevented by 1 mM dithiothreitol (Fig 2)
Dithio-threitol reduced the binding of hPYY(3–36) to a fraction
(<50%) of particulate Y2 sites at a KI value of about
3.5 mM with either the gpY2 or the rat forebrain Y2
receptor (Table 1) However, dithiothreitol did not affect
the binding to cell surface sites at up to 1 mM (Fig 2)
Pretreatment with dithiothreitol at up to 1 mM did not
affect gpY2 internalization relative to controls, and also
neutralized any decrease in receptor- linked Y2 ligand
internalization by PAO (Fig 2)
Effects of the alkylators NEM and MTSET
Effects of alkylators on availability of Y2 sites were
examined only with gpY2 receptor expressed in CHO cells
NEM, an alkylator slowly penetrating the lipid bilayer [23],
also significantly activated the monolayer Y2 sites The
increase appeared to saturate between 10 and 100 lM
(Fig 3), and was followed by a significant decrease at
300 lM, reflecting inactivation of a fraction of the Y2 sites at
high concentrations of the alkylator [14] The
restricted-access alkylator MTSET [24] did not produce a significant
stimulation of the gpY2 surface binding at 10–100 lM, and
was inhibitory at 300 lM (Fig 3) Both alkylators
essen-tially prevented internalization of hPYY(3–36) at 300 lM,
and their activity at that concentration was fully neutralized
by 1 mMdithiothreitol (Fig 3); a highly selective blockade
of internalization by NEM was also found for the
gpY1-CHO receptor (data not shown)
Activation of Y2 sites by filipin III Possible effects of the cholesterol-complexing polyene filipin III on the availability of cell-surface Y2 receptors were also assessed in gpY2-CHO cells A substantial increase in surface Y2 binding could be shown, dependent on concen-tration of the antibiotic The increase reached about twice the control level at 3 lMwas somewhat reduced at 10 lM, and then dropped to almost the control levels at 30 lM (Fig 4) In the same set of experiments, the increase in surface Y2 binding at 10 lM PAO was, as routinely observed, close to five times the control value (Fig 4) Stimulation of the binding by filipin was largely prevented
by equimolar cholesterol, and was essentially cancelled at a cholesterol concentration three times in excess to that of filipin (Fig 4) The unmasking of the Y2 sites by 10 lM PAO was not altered by equimolar cholesterol (Fig 4) Cholesterol alone at 10 lM slightly increased the surface binding of the Y2 agonist (Fig 4)
Activation of the Y2 sites by nonionic detergents or emulsifiers could not be satisfactorily studied with cell monolayers, due to loss of cell attachment With either gpY2-CHO or rat forebrain particulates, pretreatment with polyoxyethylene sorbitan emulsifiers Tween 40 (monopalm-itate) or Tween 80 (monooleate) at up to 10 mMproduced less than 10% activation (data not shown)
Dynamics of activation of the surface Y2 sites
by phenylarsine oxide indicates little accumulation due to receptor externalization
The dynamics of appearance of additional surface sites at
30 lMPAO indicated a fast activation, as the increase in the labeling by agonist peptides relative to control values
Fig 2 Unmasking of the binding sites for Y2 selective ligand
125
I-labeled hPYY (3–36) on gpY2-CHO cells by phenylarsine oxide is
prevented by dithiothreitol The cells were pretreated for 30 min at
37 Cwith 30 l M PAO with or without dithiothreitol (100 l M )1 m M ),
or with 1 m M dithiothreitol alone, washed and labeled with 125 I-labeled
hPYY(3–36) for 30 min at 37 C , followed by extraction of
surface-bound ligand with acid saline at 0–4 C(see Materials and methods).
The data are averages of three experiments In this and further graphs,
asterisks indicate differences significant vs the control cell surface
binding at the level of 95% (*) or 99% (**) confidence in Tukey t-tests,
while ampersands (&, &&) show the corresponding differences for the
internalized binding DTT, dithiothreitol.
Fig 3 Effects of two alkylating agents on surface binding and inter-nalization of125I-labeled hPYY (3–36) in gpY2-CHO cells The labeling
at 50 p M of the Y2 agonist was carried out for 40 min at 37 C , in the presence of the indicated concentrations of NEM, MTSET or DTT The separation of surface and internalized tracer was done as in Figs 1 and 2 In the same set of experiments (n ¼ 3), the surface binding
of the Y2 agonist in the presence of 10 l M PAO was 101 ± 2.8 fmolÆmg)1cell protein Significance in Tukey t-tests is indicated in Fig 2 DTT, dithiothreitol.
Trang 5saturated within 20 min at 24C, and in less than 10 min at
37C(Fig 5A) More than 50% of the total increase in
surface gpY2-CHO sites by PAO was observed within
3 min of labeling at any temperature (Fig 5A) A similar
fast activation was observed for Y2 receptors of rat
forebrain cells (not shown) This was contrasted by a
gradual, steady accumulation of surface Y1 sites in
gpY1-CHO cells at 3 lM filipin or 10 lM PAO, related to
inhibition of Y1 receptor internalization by these agents
(Fig 5B) With the gpY1-CHO receptor (which is
inten-sively internalized and recycled, as different from the gpY2
receptor expressed in the same cell type [12]), there was a
gradual relative increase (over eightfold) in labeled surface
sites between 3 and 60 min of incubation at 24Cin the
presence of either filipin III or PAO (Fig 5B)
D I S C U S S I O N
Several rhodopsin-family receptors were described as partly
cryptic, hidden, masked, or compartmentalized, for example
the pituitary LHRH [25], the protease-activated sevenfold
receptors such as the thrombin receptor [26], the
a2-adrenergic receptor reserve (60–70% of all sites [27]), and
the 5-HT1B receptor reserve (up to 90% of the total
receptor [28]) The muscarinic acetylcholine receptor, the
classic example of an aggregated rhodopsin-like receptor, is
usually examined in relation to its sequestration by agonists
However, its massive aggregation by agrins, which also
involves cell-surface dystroglucans [29], could induce a
degree of constitutive, agonist-unrelated sequestration Among the Y receptors, the hypothalamic Y2 receptors induced by estrogen show fast density changes by pro-gesterone [30], possibly related to receptor masking In this study, competition by Y2 agonist after treatment by PAO indicated an essentially normal affinity for the unmasked Y2 sites, which should derive from a constitutively sequestered membrane compartment
This work finds a considerable activation of cell mem-brane Y2 sites by a vicinal dithiol-bridging agent, and a lower activation by the alkylating agent NEM, or by the choles-terol-complexing agent filipin III With PAO, the cell-surface activation reaches 50% of the receptor numbers found in particulates derived by mechanical disruption of the cells In cell cultures, many of the surface receptors may not be readily available for interaction with larger peptidic ligands due to cell–cell interactions or interactions with the substra-tum It is therefore reasonable to assume that the arsenical would expose a majority of the sites that can be labeled in the absence of cell disruption by 34–36-residue peptides used for the Y2 labeling Lack of Y2 activation by the restricted membrane-access alkylator MTSET [24] indicates a large role for a cell membrane compartment in the masking of Y2 sites From our previous work [14], a substantial part of Y2 receptors in this compartment should be aggregated and anchored to cytoskeletal proteins, some of which contain PAO-sensitive vicinal dithiol and even trithiol motifs Activation of masked populations of surface receptor sites by PAO was shown previously for macroglobulin,
Fig 4 Unmasking of surface Y2 sites in gpY2-CHO cells by filipin III
and effect of cholesterol The cell monolayers were labeled by
125
I-labeled hNPY at 50 p M for 40 min at 37 Cat 1, 3, 10 or 30 l M
filipin III (FIII) without or with 10 l M cholesteryl hemisuccinate
(Chol), and the tracer attached to surface receptors was extracted by
acid saline at 0–4 C(see Materials and methods) Unmasking by
10 l M PAO resulted in surface binding of 90 ± 1.47 fmolÆmg)1
pro-tein without Chol, and of 89.3 ± 0.9 fmolÆmg)1protein with 10 l M
Chol (n ¼ 3) Significance vs the control binding was evaluated as in
Fig 1.
Fig 5 Comparative dynamics of labeling of Y2 sites in gpY2-CHO cells and of Y1 sites in gpY1-CHO cells in the presence of phenylarsine oxide
or filipin III The ligands (see below) were used at 50 p M for the indi-cated periods of time After removal of the unbound tracer and washing, the cells were extracted with cold acid saline to separate the cell surface-bound and internalized ligand (see Materials and meth-ods) The results are expressed as percent of control labeling The data are averages of three experiments (A) Surface labeling (ext) and internalization (int) of 125 I-labeled hPYY(3–36) in gpY2-CHO cells at
10 l M PAO The control labeling at 60 min was 20.3 ± 0.6 and 6.9 ± 0.23 (37 C), and 24 ± 0.83 and 3.3 ± 0.15 fmolÆmg)1total cell protein (24 C) for the cell-surface and internalized fraction, respectively For any time point, the elevation of Y2 binding in the presence of PAO was highly significant vs the respective control binding in Tukey t-testing (B) Surface labeling (ext) and internaliza-tion (int) of 125 I-labeled hNPY in gpY1-CHO cells at 24 Cin the presence of 10 l M PAO or 3 l M filipin III After 60 min at 24 C , the control labeling was 7.5 ± 0.23 and 37.7 ± 0.3 fmolÆmg)1total cell protein for the cell-surface and internalized fraction, respectively Note that the internalized fraction of the Y1 binding with either inhibitor was less than 5% of control values.
Trang 6transferrin and mannose-tipped glycoprotein receptors [31],
some of which are C-lectins possessing internal vicinal
dithiols [32], and could be activated by shedding or
disengaging the membrane neighbors through bridging by
PAO This study finds that the surface Y2 binding to either
CHO cells or rat forebrain cells is strongly activated by PAO
below 100 lM The Y2 receptor that contains no vicinal
dithiols [17] is inhibited by PAO only above 1 mM, i.e at
molarities many orders of magnitude in excess of those
producing the maximum unmasking of cell-surface Y2 sites
The activation of the Y2 receptor by PAO could mainly
result from alterations in arrangement of neighbors
posses-sing vicinal dithiols
The communication between the extracellular matrix
(ECM) and the actin cytoskeleton (especially the a-actinin
component) indicates a direct involvement of basal lamina
molecules such as fibronectin, collagen IV, or laminin in
cytoskeletal handling of the plasma membrane nicotinic
receptor [33] This could also apply to the Y2 receptor The
shear-stress signal transduction proposed by Kano et al
[34] could relate to the known aggregation of Y2 receptors
[14], and to an involvement of basement membrane
constituents The shear can result from PAO-imposed
bridging of vicinal cysteines in ECM molecules, as well as of
tandem dithiols in a-actinins, adaptins and selectins A role
for single intramembrane thiols could also be assumed,
based on the lack of Y2 activation by MTSET, and limited
activation by NEM, an alkylator that would access both the
exposed and the membrane-hidden thiols, but in a different
time frame [23] Examination of the activity of the alkylating
agents, however, is complicated by their partial inactivation
of Y2 binding at molarities below 1 mM([14]; this work)
A thiol/disulfide redox system could regulate cell surface
Y2 availability mainly through neighboring or interacting
membrane proteins
De-anchoring by PAO should also be considered in the
context of thiol-disulfide redox equilibria Molecules
sensi-tive to trivalent arsenicals could be sought especially among
the strongly expressed adhesion and interaction factors such
as integrins (e.g a1-integrin, a laminin and collagen
receptor), cadherins, and glypicans Adhesion system
ligands possessing multiple vicinal dithiols such as melusin
[35], and multifunctional receptor/ligand proteins, e.g
laminins, could also be modified by PAO [36], and this
might change the membrane protein arrangement in the
vicinity of Y2 sites Anchoring of selectins (e.g.L-type; [37]),
as well as of other adhesion molecules [38] can be reduced by
dithiol bridging, and this type of change might alter the
availability of Y2 sites for agonist binding
However, PAO can also act on intramembrane and
intracellular sites, as it penetrates the cell membrane with
ease, and does accumulate in the cytosol [39] Among cell
membrane molecules that can be affected by the arsenical to
alter the state of aggregation of the Y2 receptor, certain
types of phosphatases could be of importance [40] This,
however, may not be connected directly to the Y2 receptor,
which is poorly internalized in response to agonist binding
([12]; this work) Most of the activation of the Y2 sites
appears to be related to unmasking of occluded surface
receptors, and is also accomplished by shearing involved in
cell homogenization Alkylators and PAO might also
increase cell permeability, as observed in the case of
occludin proteolysis [41] Dethiolation of protein disulfides
via thioreductase could also be sensitive to PAO [42], and the loss of disulfide dynamics could be partly responsible for the observed Y2 accumulation Stretch receptors and mechanoreceptors could also participate in regulation of Y2 sites Tight junction-permeability could be increased by tyrosine phosphorylation [43], to loosen the structure of these aggregates, and PAO might act to improve the phosphorylation by inhibiting tyrosine protein phospha-tases [44]
Decrease in affinity of cell-surface Y2 sites after PAO is small, and could mainly reflect disaggregation of the sites The change should be related to modifications of the membrane environment of the receptor, as there are no vicinal cysteines in the molecule of the rodent Y2 [17] Unlike the metabotropic glutamate receptors or the adren-ergic receptors, the Y receptors, except the Y5 [45], do not contain vicinal cysteines that could impart a particular sensitivity to PAO in ligand binding Thus, the particulate Y2 has a PAO KIabove 1 mM, the Y1 of 400 lM, while the Y5 shows a KIof only about 10 lM[46] (implicating the seventh transmembrane segment in Y5 ligand binding) Various proteasome subunits possess vicinal cysteines [47], which can be modified by PAO to alter either the proteolytic function, the anchoring, or the assembly of proteasome complexes The fast activation of Y2 binding by PAO would indicate cell membrane rather than intracellular targets However, proteasomes are known to be associated with cell membrane as well as with intracellular membrane systems Intramembrane targets of PAO could also include G-protein b- and c-subunits that contain vicinal cysteines,
as well as some rab and ras G-proteins
The stimulation of Y2 binding by filipin should not result from accumulation of surface sites (as we have shown in parallel experiments for the rapidly internalizing Y1 recep-tor expressed in CHO cells), but rather from a direct unmasking due to complexing of membrane cholesterol, as cholesterol was able to abolish the effect of the polyene This may involve glycosylphosphatidylinositol anchors, known
to undergo a constitutive cholesterol-dependent sequestra-tion into early endosomes [48]
The Y2 receptor activation by alteration of sulfhydryl or cholesterol balance was found in this study for cells with quite different plasma membrane systems The CHO cells are of epithelial derivation The forebrain cells expressing the Y2 receptor might, in addition to neuronal cells [49], also include glia, in an analogy to kidney epithelia [50] The Y2 activation indicates involvement of a general mechanism that can operate across cell types to ensure rapid engage-ment of a sequestered receptor pool in response to stimuli that might range from micromechanical to oxidoreductive This segregation is not present with rapidly recycling Y1 receptors, but could be shared by other receptors that show low rates of ligand-induced internalization [12]
A C K N O W L E D G E M E N T S
This research was partly supported by NIH grants 13703 and 12844, and by State of Tennessee Educational Funds.
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