Solution NMR structure of five representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity Laurent Volpon and Jean-Marc Lancelin Laboratoire de
Trang 1Solution NMR structure of five representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity
Laurent Volpon and Jean-Marc Lancelin
Laboratoire de RMN Biomole´culaire associe´ au CNRS, Universite´ Claude Bernard – Lyon 1 and Ecole Supe´rieure de Chimie Physique & Electronique de Lyon, Villeurbanne, France
Glycosylated polyene macrolide antibiotics, as nystatins and
amphotericins, are amphiphilic structures known to exert
antifungal activity by disrupting the fungal cell membrane,
leading to leakage of cellular materials, and cell death This
membrane disruption is strongly influenced by the presence
and the exact nature of the membrane sterols The solution
structures of five representative glycosylated members, three
tetraenes (pimaricin, nystatin A1and rimocidin) and two
heptaenes (candidin and vacidin A) have been calculated
using geometric restraints derived from1H-NMR data and
random searches of their conformational space Despite a
different apparent structural order, the NMR solutions structure indicate that the hydroxyl groups all clustered on one side of the rod-shaped structures, and the glycosyl moieties are structurally conserved both in their conforma-tion and their apparent order The molecular structures afford an understanding of their selective interaction with the membrane sterols and the design of new polyene macrolides with improved activities
Keywords: antifungal antibiotics; polyene macrolides; sterol-dependant antibiotics; NMR solution structure; 1, 3-polyols
The vast family of polyenes antibiotics [1,2] includes
amphiphilic compounds mostly produced by Streptomyces
species with potent antifungal properties Polyene
macro-lides are of an authentic clinical value for efficient therapies
against animals, and human infectious diseases caused by
pathogenic fungi In particular, nystatin A1, amphotericin
B, and pimaricin (natamycin) are the most common
polyene macrolides used for the treatment of fungal
infections Due to its particular low toxicity, pimaricin
also has been used for a decade as a food preservative [3,4]
allowed in the European Union (additive E235) and
USA for preserving foods from mold contamination and
possible inherent risks of mycotoxin poisoning The
polyene macrolides target the cytoplasmic membranes of
fungi where they interact selectively with ergosterol,
causing a major disorganization of the membrane structure
[5] leading to the leakage of cellular materials and in turn
the cellular death
Depending on their molecular structures, polyene
macrolides have a more or less toxicity, in part due to a
residual interaction with cholesterol in mammalian
cyto-plasmic membranes This gives to polyene macrolides
therapies undesired hemolytic and nephrolytic side-effects
Other relevant effects assigned to some polyene
macro-lides, such as antiviral properties against several groups of
enveloped viruses [6,7] or stimulation of the immune
response at lower concentrations [8,9], have been also
reported These activities make of polyene macrolides a source of lead structures for the engineering of future molecules with improved medicinal purposes In parti-cular, the gene clusters involved in the biosynthesis of pimaricin in S natalensis [10,11] and nystastin in S nour-sei [12] have been recently cloned and new models for their biosynthetic pathways been proposed These new insights make bioengineering possible for new polyene macrolides in addition to chemical synthesis
Despite their discovery over 50 years ago, the under-standing of the selective affinity of polyenes macrolides for sterols in biomembranes has yet no experimental molecular explanation at atomic resolution The con-formational analysis of different members of the polyene family is one important step essential in understanding their structure-to-activity relationships Three-dimen-sional structures of only three polyene macrolides of disparate nature and activity, have been described to date Amphotericin B [13,14] and roxaticin [15] were solved by crystallography, while filipin III was solved using solution NMR [16]
Full stereochemical information (with the exception of one chiral center at position 42 of the vacidin A side chain, Fig 1) are available for at least five polyene macrolides that belong to a group of polyenes specifically glycosylated by mycosamine, a hexose of theD-series The glycosylation by
an amino sugar occurs near a carboxylic acidic function of the macrolide, so that these polyene macrolides are zwiter-ionic in addition to being amphiphilic Nystatin A1was the first polyene macrolide discovered [17] Its covalent struc-ture (without stereochemistry) was confirmed in 1970 [18] and 1971 [19] Pimaricin, or natamycin [20], was isolated in
1957 [21] and its covalent structure was established by Golding et al [22] Rimocidin from S rimosus was reported
in 1951 [23] and its covalent structure finally described in
1977 [24] Vacidin A, one of the main components of the aureofacin complex from S aureofaciens, belongs to the
Correspondence to J.-M Lancelin, Laboratoire de RMN
Biomole´culaire, Universite´ Claude-Bernard – Lyon 1, Domaine
Scientifique de La Doua, CPE – Lyon, 43, boulevard du 11 Novembre
1918, F-69622 Villeurbanne cedex, France.
Fax/Tel.: + 33 4 72 43 1 3 95,
E-mail: lancelin@hikari.cpe.fr
Abbreviation: ROE, rotating frame Overhauser effect.
(Received 11 March 2002, revised 17 July 2002, accepted 23 July 2002)
Trang 2aromatic macrolide group [25] Finally, candidin is a
main component of the antibiotic complex produced by
S viridoflavus[26] These five polyene macrolides have a
26-to 38-membered macrolac26-tone ring, containing a polyol
and a polyene part, which is the origin of their amphiphilic
nature, and a D-mycosamine sugar (Fig 1) Asymmetric
centers of these five glycosylated polyenes were
character-ized by of NMR spectroscopy and stereo-controlled organic
synthesis for nystatin A1[27,28], pimaricin [29,30],
rimo-cidin [31], varimo-cidin A [32] and candidin [33]
We took the advantage of the complete knowledge of the
stereochemical information of these five polyene macrolides
to study their conformation in solution, using NMR and
molecular modeling protocols used to explore the
confor-mation space of biopolymers [34] We report herein, the first
comparative solution NMR structures of these five
repre-sentative 26–38-membered polyene macrolides glycosylated
byD-mycosamine
M A T E R I A L S A N D M E T H O D S NMR experiments
Nystatin A1sample was obtained from Dr C Cimarusti, The Squibb Institute for Medical Research (Princeton, New Jersey, USA) [35] and rimocidin (Pfizer Lot #4157-47-2) from Prof Kenneth L Rinehart, University of Illinois (Urbana-Champaign, Illinois, USA) The antibiotic solu-tions were prepared under dry argon in methanol-d4 at 3–5 mMconcentration All NMR spectra were recorded at
25C on a Bruker Avance DRX 500 spectrometer (1H¼ 500 MHz) using a 5-mm (1H, 13C, 15N) triple-resonance probe head, equipped with a supplementary self shielded z-gradient coil Spectra were processed using Bruker XWINNMR and GIFA V.4 [36] software Homo-nuclear two-dimensional spectra DQF-COSY [37], TOCSY (HOHAHA) [38,39] and ROESY [40,41], were recorded with a 1.5-s recovery delay in the phase-sensitive mode using the States-TPPI method [42] as data matrices of 512 (t1)· 1024 (t2) complex data points Mixing times of
80 ms for TOCSY and 250 ms for ROESY spectra were used The spectral width in both dimensions was 3500 Hz The data were apodized with shifted sine-bell and Gaussian window functions in both F1and F2dimensions after zero-filling in the t1dimension to obtain a final matrix of 1024 (F1)· 1024 (F2) real data points Chemical shifts were referenced to the solvent chemical shift (CHD2OD,d (1H)¼ 3.31p.p.m.)
For heteronuclear spectroscopy, phase-sensitive 13 C-heteronuclear single quantum coherence [43] were recorded with a 1.5-s recovery delay using the echo-antiecho method [44] The coherence pathway selection was achieved by applying pulsed-field gradients as coherence-filters [45,46] The FID was collected as a data matrix of 512 (t1,
13C)· 1024 (t2, 1H) complex data points and 150 scans per t1increment Spectral widths were 3500 Hz in F2and
17450 Hz in F1with carrier frequencies at 3.7 and 70 p.p.m., respectively
For the other three polyenes, the NMR data at
1H¼ 300 MHz were taken from the literature where complete1H-NMR assignment and experimental restraints are available Pimaricin was studied in methanol-d4[29,30], candidin in a methanol-d4/pyridine-d5/DMSO-d6(2 : 2 : 1 , v/v) mixture [33], vacidin A in a pyridine-d5/methanol-d4
(9 : 1, v/v) mixture [32]
Experimental NMR restraints For nystatin A1, pimaricin, rimocidin and vacidin A, all the interproton-distance restraints between non J-coupled pro-tons, are derived from the two-dimensional homonuclear ROESY experiments Interproton restraints were classified into three categories Upper bounds were fixed at 2.8, 3.3 and 4.0 A˚ for strong, medium and weak correlations, respectively For candidin, each of the ROE correlations were considered as weak correlations as no information concerning the ROEs relative intensity were given [32] A lower bound was fixed at 1.8 A˚, which corresponds to the sum of the hydrogen van der Waals’ radii The intensity of a
Hi) Hi+2ROEs within the polyene part was considered as reference intensity for strong correlations [29,30] Pseudo atom corrections [47] of the upper bounds were applied for
Fig 1 Molecular structures (A) Pimaricin (B) nystatin A1(C)
rimo-cidin (with R 1 : CH 2 –CH 3 ; R 2 : CH 2 –CH 2 –CH 3 ) (D) candidin and (E)
vacidin A R or S absolute configurations are indicated for asymmetric
centers Carbons are numbered according to their position in the
macrolide sequence Primed indices are assigned to the D -mycosamine
glycoside on the right.
Trang 3distance restraints involving the unresolved methylene and
methyl protons (+1A˚) For nonstereospecifically assigned
but spectroscopically resolved diastereotopic methylene
protons, the interproton distances were treated as single
(Ær)6æ))1/6 average distances When possible, H–C–C–H
dihedral angle were restrained to dihedral domains
accord-ing to the different 3JHC,CH coupling constants measured
using optimized Karplus dihedral relations [16] When
different or very large domains were deduced, some of them
could be further restricted from intermediate structure
calculations without dihedral restraints If the resulting
models with acceptable energy (see Results) gave a
parti-cular dihedral value compatible with the 3JHC,CH, the
corresponding restraint was applied in a narrower domain
The smallest final dihedral domains were not more restricted
than an arbitrary value of ± 20 (Table 3) in case of a
correct match between the dihedral angles and the measured
couplings If the dihedral value in the intermediate models
was too dispersed, no further restriction was applied
Structure calculations
Models were calculated using theX-PLORsoftware version
3.851[48] as previously described [16] Initial atomic
coordinates and structure files for each polyene macrolides
were generated step by step (given as supporting
informa-tion) from the X-PLOR libraries and topology files of
different parts of other molecules taken from the Protein
Data Bank [49] For each molecule, the atoms involved in
of the lactone function (C–CO–O–C) were maintained in a
plane The hemi-ketal 6-membered rings of the macrolides
were maintained in a chair conformation by definition of
suitable improper angle restraints The results were
visu-alized using the programMOLMOLversion 2.4 [50] Starting
from 30 randomized coordinates, the sampling of the
conformational space was performed following a simulated
annealing protocol (random SA) proposed by Nilges et al
[51] The simplified allhdg.pro force field of X-PLOR was
used The nonbonded van der Waals’ interactions were
represented by a simple repulsive quadratic term [34,51]
The experimental distance restraints were represented as
a soft asymptotic potential, and electrostatic interactions
were ignored The force constant associated with the
distance restraints was kept to 50 kcalÆmol)1ÆA˚)2
through-out the protocol One cycle of random SA consisted of
1500 steps of 3 fs at 1000 K followed by 3000 cooling steps
of 1fs from 1000 K to 100 K At the end, each structure
was subjected to 1500 steps of conjugate gradient energy
minimization
R E S U L T S
NMR assignments of nystatin A1 and rimocidin in
methanol-d4
The structure-specific assignment of the 1H and 13C
resonances of nystatin A1and rimocidin in neat
meth-anol-d4(Table 1) were carried out based on the
identifica-tion of various unambiguous resonances These signals were
used as starting points for the complete assignments In
particular, for nystatin A1, starting points were: (a) the
well-defined scalar correlations between the two methylene
protons at C28 and C29 with their neighboring CH (Fig 2);
(b) the narrow resonance of the anomeric proton H1¢ of the
D-mycosamine which is located near d 4.6 p.p.m., and weakly dependent upon the solvent or polyene macrolide nature (Fig 3); and (c) methyl, ethyl or propyl resonances in the aliphatic region (d 0.95–1.25 p.p.m.) located near the lactone function
NMR-derived geometric restraint
In addition to the regular H–C–C–H dihedral restraints derived from the 3J coupling constants, a particular geometric restraint could be applied for the structure calculation of pimaricin Indeed, a long-range coupling constant4JHO9,H10a¼ 0.5–1Hz was already non ambigu-ously assigned [30] to a specific coplanar disposition of the H10a–C10–C9–O9–H9 atoms in the so-called W-arrange-ment [52] The dihedral angle restraints derived from NMR data in neat methanol for nystatin A1and rimocidin are summarized in Table 2 Due to some spectrally degenerate proton resonances in the polyene and the polyol regions, complete extraction of the J coupling constants and the ROE information was not possible The total number of interproton distance restraints derived from the NMR data was 21, 22, 25, 20 and 50 distance restraints for pimaricin (data from [30]), nystatin A1(this study, Table 1), rimocidin (this study, Table 1), candidin (data from [33]) and vacidin
A (data from [32]), respectively
Structure calculations for the five glycosylated macrolide polyenes
From the 30 structures calculated, 21, 25, 29, 25 and 29 were retained, respectively, for pimaricin, nystatin A1, rimocidin, candidin and vacidin A on the basis of their low experimental and nonexperimental potential energies (Ftotal< 1 2 kcalÆmol)1) The models had no ROE viola-tions greater than 0.1A˚ nor dihedral violaviola-tions greater than 5 Structural statistics are given in Table 3
Structural analysis of pimaricin The superposition for a minimum rmsd of the pimaricin heavy atoms led to a single type of main-chain conforma-tion (Fig 4A, left) This is likely due in part to the particular macrolactone ring of pimaricin, which is the smallest of the series studied here This can afford an intrinsic constraint leading to a single conformer under the NMR restraint The lactone function with the conjugated C2–C3 double bond, the C4–C5 epoxide function, the chair conformation of the C9–C13 heterocycle and the conjugated tetraene C16–C23, altogether form a tightly constrained molecular topology This topology, in addition to the experimental restraints deduced from ROEs (Table 1) and3Jinformation (Table 2) give a very high apparent structural order The analysis of the model ensemble indicates, a possible hydrogen bond between the hydrogen of OH9 (HO9) and the oxygen of OH7 (OH7) which can contribute to the stabilization of the C7–C9 region of the macrolide
Structural analysis of nystatin A1 The spectral degeneracy of1H resonances in the polyol as well as in the polyene parts, precluded the derivation of
Trang 4structural restraints within and between these two structural
regions Therefore, the 25 final models of nystatin A1
appeared well ordered in only two regions: the C11–C27
which is used for the superposition for a minimal atomic
rmsd in Fig 4B (left) and in Table 3, and the C30–C1
segment near the lactone function An apparent strong
structural and local disorder is present in the C2–C10 polyol
segment as well as for the two methylenes C28 and C29 at
the junction between the tetraene C20–C27 and the diene C30–C33 The absence of experimental restraints in these two regions and the conformational space allowed to the C2–C10 and C28–C29 segments give a strong apparent disorder as shown in Fig 4 Correlated to this feature, from the six hydroxyl and the carbonyl of the C1–C13 segment of nystatin A1, only the two hydroxyl groups at C11 and C13 are hydrogen bonded in the models (Fig 4B, left) Other
Table 1 1 H and 13 C NMR assignments and ROEs of nystatin A1 and rimocidin (in MeOH-d 4 , 25 °C).
Nystatin A1
Position
d 1Ha
(p.p.m.)
d 13Ca (p.p.m.) ROEs b
Rimocidin Position
d 1Ha (p.p.m.)
d 13Ca (p.p.m.) ROEs b
H18 d
, H1¢ e
, H2¢ e
, H36¢ d , H37 e
, H5¢ c
a
Accuracy of the chemical shifts measured are ± 0.02 p.p.m and ± 0.2 p.p.m., respectively.bThe ROEs connectivities are listed once according to the proton having the lower number Intensity of the ROEs are strong ( c ), medium ( d ) or weak ( e ) f Refers to the pseudo-axial (ax) or pseudo-equatorial (eq) orientation of these protons relative to the average plane of the macrocycle.
Trang 5hydrogen bonds between the other hydroxyl groups appear
only erratically
Structural analysis of rimocidin
The small number of restraints that could be applied in the
region of C4–C8 segment and for the two aliphatic side
chains (Table 2; Fig 1) yielded a number of different
possible conformers in the C27–C8 polyol segment
(Fig 4C, left) The CO groups of C1and C5 are spread
into two major conformations, which are near mirror
images each from the other A hydrogen bond between
hydroxyl groups on C9 and C11 (HO11–OH9) was detected
in 11 out of the 29 models
Structural analysis of candidin
As for rimocidin, the polyol part appears the most disordered (Fig 4D, left) However, for the C1–C7 segment, the two hydroxyl groups on C3 and C5 are, respectively, hydrogen bonded with the CO groups of C1and C7 in the majority of the models For the C10–C13 segment, the hydroxyl groups on C11 and C13 form alternatively a
HO13–OH1 1 or a HO11–OH13 hydrogen bond The absence
of information concerning the ROEs relatives intensities (Material and methods; and [33]) is most likely the origin of moderate order of the mycosamine moiety as this region is structurally conserved compared to the other polyene macrolides of this study (Fig 4, right)
Structural analysis of vacidin A The number of experimental restraints available [32] was large for vacidin A, and the 29 final models appear well ordered, except for the aromatic side chain which is spread
in random conformations (Fig 4E, left) As for the four previous polyene macrolides, no experimental information
Table 2 Angle restraints deduced (see the text) from J coupling constants for nystatin A1 and rimocidin (500 MHz) in MeOH-d 4 , 25 °C.
J coupling constantsa Angle applied for the calculation
3 J H17–H18eq ¼ 1 Hz H17–C17–C18–H18eq ¼ )90 ± 20
3
J H18ax–H19 ¼ 1.5 Hz H18ax.–C18–C19–H19 ¼ +90 ± 20
3 J H19–H20 ¼ 8.0 Hz H19–C19–C20–H20 ¼ 180 ± 40
3 J H33–H34 ¼ 8.5 Hz H33–C33–C34–H34 ¼ 180 ± 30
3
3 J H13–H14 ¼ 10.7 Hz H13–C13–C14–H14 ¼ 180 ± 20
3
3
J H15–H16eq ¼ 1 Hz H15–C15–C16–H16eq ¼ )90 ± 20
3 J H16ax.–H17 ¼ 1 Hz H16ax.–C16–C17–H17 ¼ + 90 ± 20
3 J H17–H18 ¼ 8.5 Hz H17–C17–C18–H18 ¼ 180 ± 20
a
Accuracy of the J coupling constants measured is ± 0.2 Hz.
Fig 2 Ethylenic-to-aliphatic region (F 2 , F 1 axis) of the phase sensitive
DQF-COSY spectrum of nystatin A1, 3 m M in MeOH-d 4 recorded at
500 MHz and 25 °C The F 1 noise strip at d 3.31and 4.87 p.p.m are
due to residual signals of the solvent The two numbers indicated near
the cross peaks correspond to the numbering indicated in Fig 1of the
protons correlated in the F 1 and F 2 axis, respectively.
Fig 3. 1H NMR spectrum of nystatin A1, 3 m M in MeOH-d 4 recorded
at 1 H-500 MHz and 25 °C Resonance lines are labeled according to the hydrogen numbering indicated in Fig 1 Asterisks indicate residual signals of the solvent (d 3.31and 4.87 p.p.m.).
Trang 6are available about the conformation of hydroxyl groups
as they are fully exchanged with the solvent (pyridine-d5/
methanol-d4(9 : 1, v/v) mixture) However, the
conforma-tion of the macrolide backbone in the polyol part and the
1,3-syn configuration of the hydroxyl groups from C7 to
C15 allow a hydrogen bond network involving HO7 to
OH1 5 or HO15 to O5 in the majority of the models The
O38-C4 segment is little more disordered and two
confor-mations were found for the lactone group, which are mirror
images each from the other as for rimocidin
D I S C U S S I O N Solution structures of five polyene macrolides, glycosylated
by D-mycosamine have been calculated under NMR-derived geometric restraints The solubilities of polyene macrolides are too low in water for NMR purpose and the NMR studies were carried out in various polar organic solvents The five glycosylated polyene antibiotics chosen, are representative of the polyene macrolide family due to their pronounced antifungal activities and their different
Table 3 Structural statistics for the pimaricin and nystatin A1.
Cartesian coordinate rmsd (A˚) vs the average geometric structurea
Potential energiesb in kcalÆmol)1calculated from X-PLOR – allhdg.pro
F total 11.86 (± 0.53) 7.85 (± 0.42) 8.37 (± 0.50) 6.51 (± 0.58) 9.11 (± 0.72)
F angle 7.29 (± 0.23) 6.32 (± 0.20) 5.48 (± 0.21) 5.08 (± 0.32) 6.23 (± 0.38)
a rmsd are calculated for backbone heavy atoms (C) without the side chains (in bracket are given the segment corresponding to the rmsd value) b F bond is the bond-length deviation energy; F angle is the valence angles deviation energy; F impr deviation energy for the improper angles used to maintain the planarity of certain groups of atoms; F VDW is the van der Waals energy function; F roe is the experimental ROE function calculated using a force constant of 25 kcalÆmol)1ÆA˚)2in the case of the CHARMM22 force field, and F cdih is the experimental function corresponding to the violation of the dihedral angle restraints In bracket are given the rmsd for certain energetic terms.
Fig 4 Stereoviews of the final NMR models Left: pimaricin (A), nystatin A1(B), rimocidin (C), candidin (D) vacidin A (E) Right: Views
of the NMR ensembles, seen along the long axis of the polyene with the D -mycosamine front (indicated with an arrow) The bonds of the hydroxyl groups are represented with bold lines Carbons are labelled according to their numbering indicated in Fig 1 Models are superposed for a minimum rmsd as indicated
in Table 3.
Trang 7macrolactone ring sizes Two of them, nystatin A1and
pimaricin, are used for human therapies against pathogenic
fungi Pimaricin is the smallest molecule with a
26-membered ring and vacidin A the largest with a
38-membered macrolactone ring
The five NMR ensembles have different apparent
struc-tural order: nystatin A1appears (Fig 4) the most
disor-dered Due to the lack of direct evidence of conformational
averaging, the apparent disorder in the nystatin models
cannot be discussed in terms of structural dynamics The
unrestricted two saturated carbons C28 and C29, are
however, a possible source of conformational variation by
rotation around the axis of the saturated C28–C29 bond
The terminal region C34 to C37 region next to the diene
appears well ordered locally and relative to the C30–C33
diene as previously observed [28] The other four polyene
macrolides do not have these saturated carbons within their
polyene parts These polyenes are fully conjugated and are
consequently more conformationally restricted The planar
conjugated polyenes are certainly the important elements
contributing to the high structural order found in the
models of pimaricin, rimocidin, candidin and vacidin A
The extended polyenes constrain the polyol segments of the
macrolactone ring to adopt an almost linear staggered
conformation to satisfy the ring closure Altogether, this
gives rise to the rod-shaped amphiphilic structure, common
to all polyene macrolides We should note in addition, that
the staggered and extended conformation of regular syn
1,3-polyol motifs was proven stable only in apolar media where
intramolecular regular hydrogen bonds were found to
stabilize this sort of conformation [16,35] In the presence of
competing interactions with polar, protic or aprotic
sol-vents, van der Waals repulsion dominate and the
1,3-polyols twist to form more stable gauche conformers
The case of the nystatin A1is interesting regarding its
interaction with the sterols and its incorporation into
membranes Unlike to filipin III, a polyene antibiotic
belonging to the group of nonglycosylated polyene
macro-lides with well-defined conformation [16], the penetration
of nystatin [53], as well as amphotericin B [54], into a
dilauroylphosphotidylcholine membrane is not possible
without the presence of sterols The conformational
variability in the nystatin models gives a better overall
solvent accessibility to the hydroxyl groups in the C1–C17
region We note that this feature correlates to the 10-fold
greater solubility in methanol of nystatin A1relative to
amphotericin B Amphotericin B is structurally very close
to nystatin A1 It differs basically by a fully conjugated
heptaene segment instead of the potentially flexible polyene
motif of nystatin A1 Keeping in mind the greater
acces-sibility of the hydroxyls, we hypothesize that nystatin A1
would only insert into a membrane containing sterols after
the formation of nystatin-sterols complexes at the
mem-brane surface Such complexes would then yield more rigid
molecular edifices, less solvated by water, and in turn more
favorable to the antibiotic insertion in the membrane
A common feature appears upon comparison of the five
polyenes macrolide solution structures To illustrate this, we
have represented in Fig 4 (right) the overlays of the
different NMR ensembles The structures are seen from
the zwiterionic-head, in the polyene plane common to each
antibiotic From this orientation, we observed that the
hydroxyl groups are clustered on one side of the plane, or
even on a single axis, parallel to the long axis of the macrolide (pimaricin and vacidin, Fig 4, right) This correlates also very well with the crystal structure of amphotericin B [14] and the filipin III structure [16] Another structural character shared by the five macrolides,
is theD-mycosamine glycosyl moities always located on the opposite side of the polyene plane
Noteworthy, these common structural features are conserved, regardless of the solvent used to collect the NMR data The solvents include neat methanol for pimaricin, nystatin A1and rimocidin ([29,30] and this study); a ternary mixture of methanol/pyridine/DMSO (2 : 2 : 1) for candidin [33]; and a binary mixture of pyridine/methanol (9 : 1) for vacidin A [32] Clearly, the polyene macrolides share a specific topology of the polar hydroxyls due to a strong intrinsic geometric constraint This constraint is independent of the solvent and relies on the structure of the macrolide itself The common struc-tural feature is then likely conserved when the polyenes are transferred from the aqueous compartment to the biomembranes
The five NMR solution structures described here, are important steps in the rationalization of their selective affinity for sterols in biomembranes, and in the design of new polyene macrolides [55] with improved properties
A C K N O W L E D G E M E N T S
L.V is recipient of a PhD fellowship 1998–2001 from the French Ministe`re de l’Education Nationale de la Recherche et de la Technologie We thank Dr C Cimarusti, The Squibb Institute for Medical Research, Princeton, New Jersey and Prof K L Rinehart, University of Illinois at Urbana-Champaign, Illinois, USA for the generous gift of nystatin A1and rimocidin, respectively.
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