Bệnh dịch tả heo châu Phi là bệnh truyền nhiễm do virus gây ra. Nghiên cứu được thực hiện nhằm khảo sát đặc điểm lâm sàng và chẩn đoán bệnh dịch tả heo Châu Phi tại một số tỉnh Đồng bằng sông Cửu Long.
Trang 1%ӝP{Q7K~\.KRD1{QJQJKLӋS7UѭӡQJĈҥLKӑF&ҫQ7Kѫ
&KLFөF7K~\YQJ9,,
KHẢO SÁT ĐẶC ĐIỂM LÂM SÀNG VÀ CHẨN ĐOÁN BỆNH DỊCH TẢ HEO
CHÂU PHI TẠI MỘT SỐ TỈNH ĐỒNG BẰNG SÔNG CỬU LONG
3K̩P7K͓1K˱+XǤQK 1 1JX\͍Q3K~F.KiQK 1 , 1JX\͍Q7KDQK/mP 1 7L͉Q1J͕F7LrQ 2 1JX\͍Q7{Q6DQJ 1
7Ï07Ҳ7
1JKLrQFӭXÿѭӧFWKӵFKLӋQQKҵPNKҧRViWFiFÿһFÿLӇPOkPVjQJFӫDEӋQKGӏFKWҧKHRFKkX3KL '7+&3 YjFKҭQÿRiQEӋQKEҵQJNӻWKXұWUHDOWLPH3&5.ӃWTXҧ[pWQJKLӋPPүXWӯKHRQJKL QKLӉPEӋQK'7+&3EҵQJNӻWKXұWUHDOWLPH3&5FKRWKҩ\Fy PүXGѭѫQJWtQKYӟLYLUXV'7+&3 FKLӃPWӹOӋ7URQJÿyWӹOӋPүXGѭѫQJWtQKYӟLYLUXV'7+&3ӣWӍQK+ұX*LDQJOjFDRQKҩW NӃÿӃQOjWӍQK%ҥF/LrX YjWӍQK&j0DX .ӃWTXҧNKҧRViWWULӋXFKӭQJYj EӋQKWtFKFӫDKHRPҳF'7+&3FKRWKҩ\KHREӋQKFyELӇXKLӋQFiFWULӋXFKӭQJQKѭӫUNJEӓăQ VӕWFDR R& VLEӑWKRһFPiXӣPNJLPLӋQJ [XҩWKX\ӃWYQJWDLP}PGD KRNKyWKӣ WLrXFKҧ\SKkQ OүQPiX FiFEӋQKWtFKQKѭOiFKVѭQJWRVүPPjX[XҩWKX\ӃW WKұQ[XQJKX\ӃW[XҩWKX\ӃW SKәL[XҩW KX\ӃWWөKX\ӃW [XҩWKX\ӃWUXӝWJLjUXӝWQRQ KҥFKDPLGDQYjKҥFKOkPEDPjQJWUHRUXӝWVѭQJ[XҩW KX\ӃW7ҫQVXҩW[XҩWKLӋQWULӋXFKӭQJVӕWFDR R& FDRQKҩW NӃÿӃQOj[XҩWKX\ӃWP}P WDLGD ӫUNJEӓăQ 7ҫQVXҩW[XҩWKLӋQEӋQKWtFKOiFKVѭQJWRVүPPjX[XҩWKX\ӃW FDRQKҩW FKLӃPWӹOӋ NӃÿӃQOjKҥFKDPLGDQYjKҥFKOkPEDPjQJWUHRUXӝWVѭQJ[XҩWKX\ӃW YӟLWӹOӋOҫQOѭӧWOjYj7yPOҥLEӋQK'7+&3FyQKLӅXWULӋXFKӭQJYjEӋQKWtFKÿD GҥQJQJRjLYLӋFFăQFӭYjRQKӳQJÿһFÿLӇPQj\FҫQSKӕLKӧSYӟLFKҭQÿRiQEҵQJNӻWKXұWUHDOWLPH 3&5ÿӇFKҭQÿRiQEӋQKPӝWFiFKFKtQK[iF
7ͳNKyD 'ӏFKWҧKHRFKkX3KLWӍQK%ҥF/LrXWӍQK&j0DXWӍQK+ұX*LDQJÿһFÿLӇPOkPVjQJ
UHDOWLPH3&5
Surveillance on clinical symptoms and diagnosing African swine fever
(ASF) in some provinces of the Mekong Delta
Pham Thi Nhu Huynh, Nguyen Phuc Khanh, Nguyen Thanh Lam, Tien Ngoc Tien, Nguyen Ton Sang
SUMMARY
The study was carried out to investigate the clinical symptoms of African swine fever (ASF) and
to detect African swine fever viruses by using realtime-PCR The result of testing 105 samples from the pigs suspecting ASFV infection by realtime-PCR showed that there were 89 samples positive with ASFV, accounting for 84.76% The highest rate of ASFV positive samples was in Hau Giang province (94.20%), followed by Bac Lieu province (83.40%) and Ca Mau province (67.74%) The result of investigating the clinical symptoms and lesions of the ASFV infection pigs showed that the disease pigs presented several manifestations, such as: depression, loss of
Trang 2appetite, high fever (body temperature 40-42oC), epistaxis, mucous discharged from the mouth, noses, hemorrhage in the ears, mouth, skin, coughing, dyspnea bloody diarrhea The lesions of the infection pigs included: splenomega, dark colour, hemorrhage kidney, lung, small and large intestine hemorrhage swollen mesenteric lymph and amidal nodes The occurrence frequency
of high fever was high (75.28%), followed by depression, hemorrhages in the mouth, ears and skin (55.10%), depression and loss of appetite (38.20%), splenomega, dark colour, hemorrhage (59.56%), swollen mesenteric lymph and amidal nodes (25.84%-32.58%, respectively) In conclusion, ASF showed many diverse symptoms and lesions, combination of clinical finding and diagnosis by realtime-PCR method should be used for accurate diagnosis of ASF
Keywords: African swine fever, Bac Lieu province, Ca Mau province, Hau Giang province,
clinical symptoms, realtime-PCR
,0ӢĈҪ8
%ӋQK GӏFK Wҧ KHR FKkX 3KL WrQ WLӃQJ $QK
Oj $IULFDQ VZLQH IHYHU Oj EӋQK WUX\ӅQ QKLӉP
GR YLUXV Jk\ UD OҫQ ÿҫX WLrQ ÿѭӧF P{ Wҧ EӣL
0RQWJRPHU\ 9LUXV'7+&3FyYұWFKҩW
GLWUX\ӅQGҥQJ'1$VӧLNpSWӯÿӃQ NE
YӓEӑFGҥQJ,FR[DKHGHDOWKXӝFKӑAsfarviridae
FKL $V¿YLUXV 'L[RQ Yj FV 9LUXV ÿѭӧF
WuPWKҩ\WURQJPiXFѫTXDQGӏFKEjLWLӃWWӯKHR
QKLӉPEӋQKKRһFFKӃWEӣLEӋQKQj\6ӭFÿӅNKiQJ
FӫDYLUXV'7+&3FDRQKѭQJNKҧQăQJFKӏXQKLӋW
WKҩSEӏGLӋWӣQKLӋWÿӝ&WURQJSK~WKRһF
ӣ&WURQJSK~WULrQJWURQJFiFVҧQSKҭP
WKӏWKHRVӕQJKRһFQҩXӣQKLӋWÿӝWKҩSYLUXVFy
WKӇWӗQWҥLWӯÿӃQWKiQJ $GNLQYjFV
%ӋQK '7+&3 Fy ÿһF ÿLӇP Ok\ ODQ QKDQK
[ҧ\ UD WUrQ PӑL OӭD WXәL Yj ORjL KHR KHR QKj
YjKHRKRDQJGm '7+&3Jk\WKLӋWKҥLQJKLrP
WUӑQJ YӟL Wӹ OӋ FKӃW FDR OrQ ÿӃQ =VDN
YjFV +LӋQQD\FKѭDFyYDF[LQSKzQJ
EӋQK '7+&3 KѫQ QӳD YLUXV Fy NKҧ QăQJ WӗQ
WҥL OkX QJRjL P{L WUѭӡQJ NKy NLӇP VRiW 'R
ÿyYLӋF[iFÿӏQKÿһFÿLӇPOkPVjQJFӫDEӋQK
'7+&3 YӟL FiF EӋQK WUX\ӅQ QKLӉP NKiF Oj
FҫQ WKLӃW QKҵP FKҭQ ÿRiQ EӋQK 1JRjL FKҭQ
ÿRiQOkPVjQJKLӋQQD\FyUҩWQKLӅXNӻWKXұW
JL~S FKҭQ ÿRiQ YLUXV '7+&3 QKѭ HQ]\PH
OLQNHGLPPXQRVRUEHQWDVVD\ (/,6$ LQGLUHFW
IXRUHVFUHQWDQWLERG\ ,)$ ÀXRUHVFHQWDQWLERG\
WHVW )$7 UHDOWLPHSRO\PHUDVHFKDLQUHDFWLRQ
UHDOWLPH3&5 3&5 7URQJ ÿy Nӻ WKXұW FKR
NӃWTXҧFKtQK[iFQKDQKFKyQJәQÿӏQKYӟLÿӝ
QKҥ\FDROjUHDOWLPH3&5 2,(
7ҥL9LӋW1DPFiFQJKLrQFӭXYӅYLUXVJk\ EӋQK'7+&3FzQKҥQFKӃ/r9ăQ3KDQYjFV JKLQKұQәGӏFKÿҫXWLrQӣ+ѭQJ<rQYjR WKiQJQăP +HRFRQYjKHRQiLFyWULӋX FKӭQJ [XҩW KX\ӃW WRjQ WKkQ YLrP NӃW PҥF Yj WLrXFKҧ\FyPiX ULrQJKHRQiLELӇXKLӋQFKiQ ăQWtPWiLYjVӕW !R& .ӃWTXҧFKҭQÿRiQ
EҵQJSKѭѫQJSKiSUHDOWLPH3&5 FiFPүX OiFK
JDQWKұQYjKҥFKEҥFKKX\ӃW WӯKHRFKӃWFKR NӃWTXҧGѭѫQJWtQKYӟLYLUXV'7+&3
'RÿyQJKLrQFӭXQj\ÿѭӧFWKӵFKLӋQQKҵP [iFÿӏQKFiFÿһFÿLӇPOkPVjQJSKӕLKӧSYӟL FKҭQÿRiQFұQOkPVjQJEҵQJNӻWKXұWUHDOWLPH 3&5WҥLFiFәGӏFKWUrQÿӏDEjQFiFWӍQK&j0DX
%ҥF/LrXYj+ұX*LDQJ
,,9Ұ7/,ӊ89¬3+ѬѪ1*3+È3 1*+,Ç1&Ӭ8
7KӡLJLDQYjÿӏDÿLӇPQJKLrQFӭX
7KӡLJLDQQJKLrQFӭXWӯWKiQJÿӃQWKiQJ
QăP WUrQ ÿӏD EjQ &j 0DX %ҥF /LrX Yj +ұX *LDQJ &KҭQ ÿRiQ PүX EҵQJ Nӻ WKXұW UHDOWLPH3&5WҥL&KLFөF7K~\YQJ9,,
ĈӕLWѭӧQJQJKLrQFӭX
&iF ÿjQ KHR ÿѭӧF QX{L WKHR KѭӟQJ Q{QJ Kӝ WUDQJ WUҥL Fy ELӇX KLӋQ EӋQK '7+&3 KHR FKӃWÿӝWQJӝWÿLӅXWUӏNK{QJWKX\rQJLҧPKRһF NK{QJÿLӅXWUӏ/RҥLPүXEӋQKSKҭP PүXSKӫ WҥQJ KҥFKOiFKSKәL PүXKX\ӉQGӏFK PiX
&KҭQÿRiQYLUXV'7+&3EҵQJNӻWKXұW UHDOWLPH3&5
Trang 3&KҭQ ÿRiQ EӋQK '7+&3 EҵQJ Nӻ WKXұW
UHDOWLPH3&5YӟLFһSPӗLÿѭӧFF{QJEӕWUѭӟF
ÿyFӫD.LQJYjFV. WKHRNKX\ӃQFiRFӫD
2,( +ӛQKӧSSKҧQӭQJYjFKXWUuQKQKLӋW
FKRSKҧQӭQJÿѭӧFP{WҧFKLWLӃWӣEҧQJYj EҧQJ.LWVӱGөQJOj3ODWLQXP4XDQWLWDWLYH 3&5 6XSHU0L[ ± 8'* ,QYLWURJHQ 7KHUPR )LVKHU6FLHQWL¿F0$86$
%ҧQJ&KLWLӃWFһSPӗLYjÿRҥQGzVӱGөQJFKRFKҭQÿRiQSKiWKLӋQ
YLUXVWKHRSKѭѫQJSKiSUHDOWLPH3&5
0)$0&&$&***$**$$7$&&$$&&&$*7*
03ULPHU ¶&7*&7&$7**7$7&$$7&77$7&*$¶
03ULPHU ¶*$7$&&$&$$*$7&*&&*7¶
%ҧQJ&KXWUuQKQKLӋWFӫDSKҧQӭQJUHDOWLPH3&5 2,(
3KѭѫQJ SKiS NKҧR ViW WULӋX FKӭQJ Yj
EӋQKWtFKWUrQKHRGREӋQK'7+&3Jk\UD
'ӵD YjR NӃW TXҧ FKҭQ ÿRiQ YLUXV '7+&3
EҵQJNӻWKXұWUHDOWLPH3&5 QKӳQJWUѭӡQJKӧS
GѭѫQJWtQKÿѭӧFÿLӅXWUDKӗLFӭXJKLQKұQÿһF
ÿLӇPOkPVjQJJӗPWULӋXFKӭQJEӋQKWtFKFӫD
WӯQJWUѭӡQJKӧSWURQJELrQEҧQJӱLYӅ&KLFөF
7K~\YQJ9,,
,,,.ӂ748Ҧ9¬7+Ҧ2/8Ұ1
7ӹOӋPүXQKLӉPYLUXV'7+&3WUrQÿӏD
EjQ&j0DX%ҥF/LrXYj+ұX*LDQJ
.LӇPWUDPүXQJKLQKLӉP'7+&3Fy
PүXGѭѫQJWtQK FKLӃPWӹOӋ
EҧQJ +ұX *LDQJ Oj ÿӏD SKѭѫQJ Fy Vӕ
OѭӧQJ PүX GѭѫQJ WtQK FDR QKҩW YӟL
PүX NӃÿӃQOj%ҥF/LrX
Yj WKҩS QKҩW Oj &j 0DX 7KHR
2,( FKҭQ ÿRiQ EӋQK '7+&3 EҵQJ
SKѭѫQJSKiSUHDOWLPH3&5FyWKӇSKiWKLӋQ '1$FӫDYLUXVWӯJLDLÿRҥQQKLӉPEӋQKUҩW VӟP Yj ÿһF ELӋW KӳX tFK WURQJ WUѭӡQJ KӧS PүX NK{QJ SK KӧS ÿӇ SKkQ OұS KRһF SKiW KLӋQ NKiQJ QJX\rQ GR TXi WUuQK EҧR TXҧQ PүX NK{QJ ÿҥW \rX FҫX 7URQJ QKӳQJ QăP TXD UHDOWLPH3&5 ÿѭӧF SKiW WULӇQ Yj F{QJ QKұQÿӇSKiWKLӋQYLUXV'7+&3WKXӝFQKLӅX NLӇXJHQFKӫQJYjÿӝFOӵFÿDGҥQJ $JHUR YjFV )HUQDQGH]3LQHURYjFV LQJYjFV 7LJQRQYjFV =VDN Yj FV &KҭQ ÿRiQ '7+&3 EҵQJ Nӻ WKXұW UHDOWLPH3&5 ÿѭӧF NKX\ӃQ FiR WKHR 2,( 2,( LQJ Yj FV Yj UHDOWLPH3&5EәVXQJÿҫXGzTXDQJSKә83/ 8QLYHUVDOSUREHOLEUDU\ )HUQDQGH]3LQHUR YjFV ÿDQJOjKDLSKѭѫQJSKiSÿѭӧF Vӱ GөQJ UӝQJ UmL QKҩW ÿӇ FKҭQ ÿRiQ WKѭӡQJ TX\ӣFiFSKzQJWKtQJKLӋPWKDPFKLӃXTXӕF JLDFӫD(8 *DOODUGRYjFV
Trang 4.ӃW TXҧ NKҧR ViW ӣ EҧQJ FKR WKҩ\
'7+&3 ÿDQJ Oj EӋQK ҧQK KѭӣQJ QJKLrP
WUӑQJÿӃQÿjQKHRWUrQÿӏDEjQNKҧRViWQyL
ULrQJYjFiFWӍQKÿӗQJEҵQJV{QJ&ӱX/RQJ
QyL FKXQJ 'LӉQ ELӃQ SKӭF WҥS FӫD EӋQK Yj
YLӋFFKѭDFyYDF[LQSKzQJEӋQKFNJQJOjPӝW
ÿLӇPY{FQJEҩWOӧLWURQJYLӋFSKzQJFKӕQJ EӋQK'7+&3
ĈһFÿLӇPOkPVjQJFӫDEӋQK
͇W TX̫ NK̫R ViW WUL͏X FKͱQJ E͏QK '7+&3
%ҧQJ7ӹOӋPүXGѭѫQJWtQKYӟLYLUXV'7+&3WUrQÿӏDEjQ
&j0DX%ҥF/LrXYj+ұX*LDQJ
%ҧQJ7ҫQVXҩW[XҩWKLӋQWULӋXFKӭQJWUrQKHRÿѭӧFJKLQKұQGӵDWUrQFiFWUѭӡQJKӧS ÿѭӧFFKҭQÿRiQGѭѫQJWtQKYӟLEӋQK'7+&3WҥL&j0DX%ҥF/LrXYj+ұX*LDQJ Q
.ӃWTXҧӣEҧQJFKRWKҩ\WULӋXFKӭQJFӫD
EӋQK'7+&3UҩWÿDGҥQJ7URQJÿyWULӋXFKӭQJ
OLrQ TXDQ ÿӃQ EӋQK '7+&3 EDR JӗP VӕW FDR
R& FKLӃPWӹOӋFDRQKҩWYӟL NӃ
ÿӃQOj[XҩWKX\ӃWP}PWDLGD KuQK
Yj ErQFҥQKÿyӫUNJEӓăQJKLQKұQFy
WUѭӡQJKӧS FKLӃPWӹOӋ KRNKyWKӣ
YjFKӃWQKDQKOjKDLWULӋXFKӭQJFyFQJWӹOӋ
Oj VDXFQJOjWULӋXFKӭQJVLEӑWKRһF
PiX ӣ PNJL PLӋQJ KuQK Yj WLrX FKҧ\ SKkQ
FyOүQPiXFyWӹOӋWѭѫQJÿѭѫQJQKDX OҫQOѭӧW
Oj Yj ӃW TXҧ Qj\ SK KӧS
YӟLF{QJEӕWUѭӟFÿyFӫD/r9ăQ3KDQYjFV
YӅәGӏFK'7+&3ÿҫXWLrQWҥL9LӋW1DP
FNJQJJKLQKұQKHRFyELӇXKLӋQ[XҩWKX\ӃWWUrQ
NKҳSFѫWKӇEӓăQYjVӕW !R& 7URQJÿӧW EQJSKiWӣ=LPEDEZHKHRPҳFEӋQK'7+&3 FyELӇXKLӋQVӕW R& EӓăQӫUNJGiQJÿL OҳFOѭ[XҩWKX\ӃWÿLӇPWUrQWDLYjEөQJVDXNKL NLӇPWUDKHRFKӃWJKLQKұQPiXFKҧ\WӯKұXP{Q YjOӛPNJLSKSKәL[XҩWKX\ӃWӣFiFKҥFKEҥFK KX\ӃWPjQJWUHRUXӝWWKұQYjWLP +HHUGHQYj FV 7KHR=KRXYjFV WUDQJWUҥL KHRJӗPFRQÿmFKӃWVDXWKiQJWӯNKLJKL QKұQFiFGҩXKLӋXOkPVjQJÿҫXWLrQEDRJӗP VӕWFDRӫUNJWRjQWKkQ[XҩWKX\ӃW1KӳQJELӇX KLӋQWULӋXFKӭQJFӫD'7+&3SKөWKXӝFYjRÿӝF OӵFFӫDYLUXVPұWÿӝYjÿѭӡQJOk\QKLӉPFӫD YLUXVYjRFѫWKӇKHR EӋQKÿһFWUѭQJEӣLӫUNJ EӓăQVӕWFDR!R&[XҩWKX\ӃWGD ÿһFELӋWOj
Trang 5WRjQWKkQWKѭӡQJFKӃWVDXQJj\VDXNKLFiF
GҩXKLӋXOkPVjQJÿҫXWLrQ[XҩWKLӋQ ÿӃQJLDL
ÿRҥQ FXӕL WKѭӡQJ Fy WKrP ELӇX KLӋQ NKy WKӣ
WKӣQKDQKVLEӑWKRһFPiXWӯPNJLGRSKSKәL
Jk\UDWLrXFKҧ\OүQPiXÿһFELӋW'7+&3FzQ
Jk\UDKLӋQWѭӧQJVҧ\WKDLWUrQQiLFKӱDWӹOӋWӱ
YRQJFDR 6iQFKH]9L]FDtQRYjFV
)$2 JKLQKұQFiFWUѭӡQJKӧStW QJKLrPWUӑQJWKѭӡQJJk\WӹOӋFKӃWGѭӟL FiFGҩXKLӋXOkPVjQJWKѭӡQJ[XҩWKLӋQVDX ÿӃQQJj\QKLӉPEӋQKYӟLFiFELӇXKLӋQQKѭ VӕWQKҽVX\K{KҩSVѭQJNKӟSWӯWUXQJEuQKÿӃQ QһQJ[XҩWKX\ӃWGDYjKRҥLWӱ
.͇WTX̫NK̫RViWE͏QKWtFKE͏QK'7+&3
%ҧQJ7ҫQVXҩW[XҩWKLӋQEӋQKWtFKWUrQKHRÿѭӧFJKLQKұQGӵDWUrQFiFWUѭӡQJKӧS ÿѭӧFFKҭQÿRiQGѭѫQJWtQKYӟLEӋQK'7+&3WҥL&j0DX%ҥF/LrXYj+ұX*LDQJ Q
%ҧQJFKRWKҩ\EӋQKWtFK[XҩWKX\ӃWÿѭӧF
WuP WKҩ\ ӣ KҫX KӃW FiF QӝL WҥQJ FӫD KHR Eӏ
EӋQK'7+&3 KuQK 7URQJÿyEӋQKWtFKӣ
OiFKKҥFKDPLGDQYjKҥFKOkPEDPjQJWUHR
UXӝW Oj ÿһF ÿLӇP TXDQ WUӑQJ ÿӇ FKҭQ ÿRiQ
EӋQK '+7&3 %ӋQK WtFK OiFK VѭQJ WR PjX
VүP[XҩWKX\ӃW KuQK FyWҫQVXҩWFDRQKҩW
FKLӃPWӹOӋ WURQJWәQJVӕ WUѭӡQJ
KӧS GѭѫQJ WtQK YӟL '7+&3 NӃ ÿӃQ Oj KҥFK
DPLGDQVѭQJ[XҩWKX\ӃWYӟLWӹOӋYj
KҥFKOkPEDPjQJWUHRUXӝWVѭQJ[XҩWKX\ӃW
KuQK ErQ FҥQK ÿy JKL QKұQ
ÿѭӧF WUѭӡQJKӧSFyEӋQKWtFK
[XҩW KX\ӃW UXӝW JLj KuQK UXӝW QRQ WKұQ
[XQJKX\ӃW[XҩWKX\ӃW KuQK YjSKәL[XҩW
KX\ӃWWөKX\ӃWFKLӃPWӹOӋOҫQOѭӧWOj
Yj ӃW TXҧ Qj\ SK KӧS YӟL QJKLrQ
FӭX*HYjFV VDXNKLSKkQWtFKәGӏFK
ӣKX\ӋQ6KHQEHL/LrX1LQK7UXQJ4XӕFÿm
EiRFiRFyFRQKHREӏFKӃWQKyPWiF
JLҧJKLQKұQÿѭӧFQKӳQJWәQWKѭѫQJÿLӇQKuQK
FӫD EӋQK '7+&3 Oj OiFK VѭQJ WR Yj KRҥL Wӱ
QJKLrPWUӑQJ ErQFҥQKÿyOjQKӳQJEӋQKOê
NKiFJӗP[XҩWKX\ӃWӣKҥFKDPLGDQSKәLWәQ
WKѭѫQJ ӣ KҥFK OkP ED ӣ KҫXKӑQJ Yj PjQJ WUHRUXӝW[XҩWKX\ӃWGҥGj\&iFWәQWKѭѫQJ FyWKӇTXDQViWWKҩ\ӣKHRQKLӉPYLUXV'7+&3 W\WKXӝFYjRÿӝFOӵFFӫDYLUXV'7+&3ÿһF WUѭQJ EӣL [XҩW KX\ӃW WUrQ GLӋQ UӝQJ Yj SKi Kӫ\P{EҥFKKX\ӃWWәQWKѭѫQJQJKLrPWUӑQJ ӣOiFKWKұQÿѭӡQJWLrXKyD GҥGj\UXӝWQRQ UXӝWJLj JDQYjW~LPұWSKәLYjEjQJTXDQJ ӣFiFWUѭӡQJKӧSQKҽKѫQWKӡLJLDQVӕQJNpR GjLEӋQKWtFKӣÿѭӡQJK{KҩSJӗPYLrP[ѫ SKәL NӃW GtQK PjQJ SKәL YLrP [XҩW KX\ӃW WLP Yj KRҥL Wӱ FNJQJ UҩW SKә ELӃQ 6iQFKH] 9L]FDtQRYjFV
7ӯNӃWTXҧFӫDEҧQJYjFyWKӇWKҩ\EӋQK '7+&3NK{QJSKҧLO~FQjRFNJQJELӇXKLӋQWҩW FҧFiFGҩXKLӋXOkPVjQJ&KҭQÿRiQOkPVjQJ FyWKӇNKyNKăQWURQJJLDLÿRҥQÿҫXNKӣLSKiW FӫDEӋQKKRһFNKLYӟLVӕOѭӧQJKHRPҳFEӋQK tW1JRjLFiFFăQFӭ[iFÿӏQKWӯWULӋXFKӭQJYj EӋQK WtFK EDR JӗP WuQK WUҥQJ [XҩW KX\ӃW WUrQ GLӋQUӝQJYjWӹOӋFKӃWÿӝWQJӝWFҫQSKӕLKӧSYӟL FiFNӻWKXұWSKzQJWKtQJKLӋPÿӇFyFѫVӣFKtQK [iFFKRYLӋFFKXҭQÿRiQEӋQK
Trang 64XD FKҭQ ÿRiQ FiF PүX QJKL QKLӉP YLUXV
'7+&3EҵQJNӻWKXұWUHDOWLPH3&5WӹOӋPүX
GѭѫQJWtQKӣ&j0DX%ҥF/LrXYj+ұX*LDQJOj
+HRPҳFEӋQK'7+&3ELӇXKLӋQWULӋX
FKӭQJÿLӇQKuQKQKѭVӕWFDRR&
[XҩWKX\ӃWP}PWDLGD YjFiFWULӋX
FKӭQJNKiFQKѭVLEӑWKRһFPiXӣPNJLPLӋQJ
KRNKyWKӣ WLrXFKҧ\SKkQOүQPiX FKӃWQKDQK
%ӋQKWtFKÿһFWUѭQJQKѭOiFKVѭQJWRPjXVүP
FDR QKҩW KҥFK DPLGDQ Yj KҥFK OkP
ED PjQJ WUHR UXӝW VѭQJ [XҩW KX\ӃW
Yj FiFEӋQKWtFKNKiFQKѭ[XҩWKX\ӃW
GLӋQUӝQJӣWKұQSKәLUXӝWJLjUXӝWQRQ%ӋQK
'7+&3GLӉQELӃQSKӭFWҥSYjNKyNLӇPVRiW
OjPӕLÿHGӑDOӟQFKRQӅQFKăQQX{LKHRӣ9LӋW
1DPQyLFKXQJYjÿӗQJEҵQJV{QJ&ӱX/RQJ
QyL ULrQJ 3KiW KLӋQ VӟP GӏFK EӋQK JyS SKҫQ
TXDQWUӑQJWURQJYLӋF[ӱOêYjKҥQFKӃVӵOk\
ODQFӫDEӋQK'RÿyNӃWTXҧQJKLrQFӭXOjFѫVӣ
EѭӟFÿҫXÿӇFKҭQÿRiQKHRPҳFEӋQK'7+&3
/ͥLF̫P˯Q Ĉ͉WjLQj\ÿ˱ͫFK͟WUͫEͧL&KL
FͭF7K~\YQJ9,,W̩L&̯Q7K˯&KLFͭF7K~\ FiFW͑QK&j0DX%̩F/LrX+̵X*LDQJ
Ĉ͉WjLQj\ÿ˱ͫFK͟WUͫEͧL'iQQkQJF̭S WU˱ͥQJĈ̩LK͕F&̯Q7K˯913E̹QJQJX͛Q Y͙QYD\2'$WͳFKtQKSKͯ1K̵W%̫Q
7¬,/,ӊ87+$0.+Ҧ2
$JHUR 0 )HUQiQGH] - 5RPHUR / 6iQFKH]0DVFDUDTXH&$ULDV06iQFKH] 9L]FDtQR -0 +LJKO\ VHQVLWLYH 3&5 DVVD\IRUURXWLQHGLDJQRVLVRI$IULFDQVZLQH
IHYHU YLUXV LQ FOLQLFDO VDPSOHV Journal of
&OLQLFDO0LFURELRORJ\ ±
$GNLQ $ &REXUQ + (QJODQG 7 +DOO 6+DUWQHWW(0DURRQH\&:RROGULGJH 0 :DWVRQ ( &RRSHU - &R[ 7 DQG 6HDPDQ 0 5LVN DVVHVVPHQW IRU WKH LOOHJDO LPSRUW RI FRQWDPLQDWHG PHDW DQG PHDW SURGXFWV LQWR *UHDW %ULWDLQ DQG WKH VXEVHTXHQW H[SRVXUH RI *% OLYHVWRFN ,,5$ )RRW DQG 0RXWK 'LVHDVH )0'
+uQK+HRFK͗WGR
E͟QK'7+&3[X̽W
KX\͗WQ͏QJͷGDG̓Q
ÿ͗QWtPWiLWRjQWKkQ
+uQK+HR[X̽W
KX\͗WQ͏QJͷWDL
YjP}P
+uQK+HRFK͗WFy
EL͛XKL͟QFK̻\PiX
PNJLYjP͇W
+uQK;X̽W KX\͗WQͱLW̹QJ FͿDKHRQKL͝P '7+&3
+uQK/iFKPjX
V̓PV́QJWR[X̽W
KX\͗W
+uQK+̹FKOkPED
PjQJWUHRUXͱWV́QJ
[X̽WKX\͗W
+uQK5XͱWJLj
[X̽WKX\͗W
+uQK7KͅQ V́QJ[XQJKX\͗W [X̽WKX\͗W
Trang 7IHYHU $6) 6ZLQH9HVLFXODUGLVHDVH 69'
9HWHULQDU\/DERUDWRULHV$JHQF\1HZ+DZ
'L[RQ /. (VFULEDQR -0 0DUWLQV &
5RFN '/ 6DODV 0/ :LONLQVRQ 3-
$VIDUYLULGDH ,Q 9LUXV WD[RQRP\ WK
UHSRUW RI WKH ,&79 )DXTXHW &0 0D\R
0$ 0DQLORII - 'HVVHOEHUJHU 8 %DOO
/$ (GV Elsevier/Academic Press
/RQGRQ8.±
)$2 $IULFDQ VZLQH IHYHU 'HWHFWLRQ
DQGGLDJQRVLV$QLPDOSURGXFWDQGKHDOWK
)HUQDQGH]3LQHUR - *DOODUGR & (OL]DOGH
05REOHV$*RPH]&%LVKRS5+HDWK
/&RXDF\+\PDQQ()DVLQD)23HOD\R
9 6ROHU $ $ULDV 0 0ROHFXODU
GLDJQRVLV RI $IULFDQ VZLQH IHYHU E\ D QHZ
UHDOWLPH 3&5 XVLQJ XQLYHUVDO SUREH OLEUDU\
7UDQVERXQG(PHUJLQJ'LVHDVHV ±
*DOODUGR & 6ROHU $ 5RG]H , 1LHWR
5 &DQR*yPH] & )HUQDQGH]3LQHUR
- $ULDV 0 $WWHQXDWHG DQG QRQ
KDHPDGVRUELQJ QRQ+$' JHQRW\SH ,,
$IULFDQVZLQHIHYHUYLUXV $6)9 LVRODWHGLQ
(XURSH/DWYLDTransbound Emerging
'LVHDVHV ±
+HHUGHQ-90DODQ.*DGDJD%0DQG
6SDUJR505HHPHUJHQFHRI$IULFDQ
VZLQH IHYHU LQ =LPEDEZH Emerging
,QIHFWLRXV'LVHDVHV
LQJ '3 5HLG 60 +XWFKLQJV *+
*ULHUVRQ 66 :LONLQVRQ 3- 'L[RQ
/. %DVWRV $' 'UHZ 7:
'HYHORSPHQW RI D 7DT0DQ 3&5 DVVD\ ZLWK
LQWHUQDODPSOL¿FDWLRQFRQWUROIRUWKHGHWHFWLRQ
RI $IULFDQ VZLQH IHYHU YLUXV Journal of
virological methods ±
0RQWJRPHU\ 5( 2Q D IRUP RI
VZLQH IHYHU RFXUULQJ LQ %ULWLVK (DVW$IULFD
HQ\D &RORQ\ Journal of Comparative
Pathology.
2,( $¿ULFDQ VZLQH IHYHU 0DQXDO
RI GLDJQRVWLF WHVWV DQG YDFFLQHV WHUUHVWULDO
DQLPDOV &KDSWHU
/H93-HRQJ'*<RRQ6:.ZRQ +07ULQK7%11JX\HQ7/%XL 771 2K - LP -% &KHRQJ 0 7X\HQ19%DH(9X77+<HRP0 6RQJ'1D: 2XWEUHDNRI$IULFDQ
VZLQH IHYHU 9LHWQDP Emerging
,QIHFWLRXV'LVHDVHV
6iQFKH]9L]FDtQR -0 /DGGRPDGD$ DQG
$ULDV0 $IULFDQVZLQHIHYHUYLUXV
'LVHDVHVRI6ZLQH
*H6/L-)DQ;/LX)/L/:DQJ 45HQ-%DR&/LX+:DQJ<X/LX
<R=KDQJ7L;X;L:X=:DQJ 0ROHFXODU &KDUDFWHUL]DWLRQ RI $IULFDQ
VZLQH IHYHU YLUXV &KLQD Emerging
,QIHFWLRXV'LVHDVHV
7LJQRQ0*DOODUGR&,VFDUR&+XWHW ( 6WHGH <9 ROEDVRY ' 0LD*0' 3RWLHU0)/ %LVKRS 53 $ULDV 0 RHQHQ ) 'HYHORSPHQW DQG LQWHU ODERUDWRU\ YDOLGDWLRQ VWXG\ RI DQ LPSURYHG QHZ UHDOWLPH 3&5 DVVD\ ZLWK LQWHUQDO FRQWUROIRUGHWHFWLRQDQGODERUDWRU\GLDJQRVLV
RI $IULFDQ VZLQH IHYHU YLUXV Journal of
virological methods. ± ±
=KRX ; /L 1 /XR < /LX < 0LDR ) &KHQ 7 =KDQJ 6 &DR 3 /L ; 7LDQ 4LX ++X 5 (PHUJHQFH RI $IULFDQ VZLQH IHYHU LQ &KLQD
7UDQVERXQGDU\DQG(PHUJLQJ'LVHDVHV
=VDN/%RUFD095LVDWWL*5=VDN$ )UHQFK 5$ /X = XWLVK *) 1HLODQ -* &DOODKDQ -' 1HOVRQ :0 5RFN '/3UHFOLQLFDOGLDJQRVLVRI$IULFDQ VZLQH IHYHU LQ FRQWDFWH[SRVHG VZLQH E\ D
UHDOWLPH 3&5 DVVD\ Journal of Clinical
0LFURELRORJ\ ±
1Jj\QKұQ
1Jj\SKҧQELӋQ
1Jj\ÿăQJ