Results HepG2 cells have greater pyruvate requirements under hypoxic conditions Pyruvate content was quantified in cell culture medium under normoxic and hypoxic conditions, and in the ab
Trang 1reoxygenation in hepatocellular carcinoma cells
3Emilie Roudier*, Christine Bachelet and Anne Perrin
Unite´ de Biophysique Cellulaire et Mole´culaire, IFR ‘RMN biome´dicale: de la cellule a` l’homme’, CRSSA, BP 87, La Tronche, France
Pyruvate, as well as lactate, is an end-product of
gly-colysis Its production is enhanced in tumor cells,
where high rates of aerobic glycolysis, historically
known as the Warburg effect, are observed [1] It is
only lately that pyruvate has been described as playing
an important role in cancer progression First of all,
alterations in components of pyruvate metabolism
have been reported in tumor cells, and appeared to
increase cancer cell proliferation [2,3] Moreover,
recent evidence supports a novel role of pyruvate in
metabolic signaling in tumors Pyruvate has been
reported to promote hypoxia-inducible factor (HIF-1)
stability and activate HIF-1-inducible gene expression
This can promote the malignant transformation and
survival of cancer cells [4,5] Pyruvate also exhibits
strong angiogenic activity in vitro and in vivo and
positively affects angiogenic processes [6] As the angiogenic switch is a crucial event in tumorigenesis, pyruvate may be important for cancer progression All together, these findings suggest that pyruvate could induce the molecular signaling usually caused by hypoxia
Chronic or transient hypoxia in the tumor is induced
by heterogeneous bloodflow resulting from impaired vascularization [7] Tumor cells are often exposed to shorter or longer periods of hypoxia or ischemia fol-lowed by reoxygenation or recirculation An adaptive response of cancer cells takes place through multi-faceted changes [8], which are mainly coordinated by HIF-1 [9] The outcome is clonal selection of the tumor cells that are most resistant and well adapted to hypoxia [10] Many alterations occur that induce
Keywords
DNA damage; glutathione; hypoxia;
pyruvate; reoxygenation
Correspondence
A Perrin, CRSSA ⁄ RBP, Unite´ de
biophysique cellulaire et mole´culaire, 24
Avenue des Maquis du Gre´sivaudan, BP 87,
38702 La Tronche Cedex, France
2 Fax ⁄ Tel: + 33 4 76 63 68 79
E-mail: aperrin@crssa.net
*Present address
De´partement de kine´siologie, Universite´ de
Montre´al, Canada
(Received 5 July 2007, revised 10 August
2007, accepted 14 August 2007)
doi:10.1111/j.1742-4658.2007.06044.x
Pyruvate is located at a crucial crossroad of cellular metabolism between the aerobic and anaerobic pathways Modulation of the fate of pyruvate,
in one direction or another, can be important for adaptative response to hypoxia followed by reoxygenation This could alter functioning of the antioxidant system and have protective effects against DNA damage induced by such stress Transient hypoxia and alterations of pyruvate metabolism are observed in tumors This could be advantageous for cancer cells in such stressful conditions However, the effect of pyruvate in tumor cells is poorly documented during hypoxia⁄ reoxygenation In this study, we showed that cells had a greater need for pyruvate during hypoxia Pyruvate decreased the number of DNA breaks, and might favor DNA repair We demonstrated that pyruvate was a precursor for the biosynthesis of thione through oxidative metabolism in HepG2 cells Therefore, gluta-thione decreased during hypoxia, but was restored after reoxygenation Pyruvate had beneficial effects on glutathione depletion and DNA breaks induced after reoxygenation Our results provide more evidence that the a-keto acid promotes the adaptive response to hypoxia followed by reoxy-genation Pyruvate might thus help to protect cancer cells under such stressful conditions, which might be harmful for patients with tumors
Abbreviations
GSH(c-glutamyl), c-glutamyl glutathione; HIF-1, hypoxia-inducible factor; PCA, perchloric acid; ROS, reactive oxygen species.
Trang 2deleterious effects, such as resistance to anticancer
treatment, tumor growth, and metastasis development
[11,12] Moreover, they allow survival under low
par-tial pressure of oxygen
One of the alterations induced by hypoxia involves
modifications of glutathione metabolism Glutathione
is an important intracellular antioxidant and redox
potential regulator that plays a vital role in drug
detoxification and in cellular protection against
damage by free radicals, peroxides, and toxins [13]
Hypoxia enhances the expression of c-glutamylcysteine
synthetase [14] and glutathione transferase [15] in
can-cer cells Such alterations of the glutathione system
can enhance survival of cancer cells
In general, hypoxia and reoxygenation may induce
important modifications to the functioning of the
antioxidative system Multilevel adaptation to oxidative
damage could then follow, tending to enhance
protec-tive mechanisms This could have beneficial effects on
protection against DNA damage [16] Pyruvate may
play a role in this adaptive response This has been
intensively studied in normal cells and tissues, such as
heart, liver, and brain Generally, this a-keto acid is
associated with protective effects against hypoxia and
reoxygenation This is mainly ascribed to its ability to
maintain redox status [17], intervening in the DNA
repair system [18–20] and restoring antioxidant
capaci-ties [21–26] This adaptive response, beneficial in the
case of normal cells and tissues, could become
deleteri-ous for tumor carriers when it takes place in malignant
cells [27,28] However, the role of pyruvate during
hypoxia is poorly documented in cancer cells
We previously showed that pyruvate could favor the
glycolytic pathway from glucose to lactate in glial and
hepatic cells underhypoxic conditions [29] We now
investigated whether such an effect might affect the
adaptive response of tumor cells to hypoxia and
reoxy-genation We particularly focused on the antioxidant
system, in particular glutathione metabolism, and on
studying DNA damage The metabolism of glutathione
and DNA breaks were investigated in hepatocellular
carcinoma HepG2 cells cultivated with or without
pyruvate during and after hypoxia
In the present study, we showed that cells had a
greater need for pyruvate during hypoxia Pyruvate
decreased DNA breaks and might favor DNA repair
We demonstrated that pyruvate was a precursor for
the biosynthesis of glutathione through oxidative
metabolism in HepG2 cells Therefore, glutathione
decreased during hypoxia, but was restored after
reox-ygenation Pyruvate had beneficial effects on
glutathi-one depletion and DNA breaks induced after hypoxia
Our results provide more evidence that a-keto acids
promote adaptative responses to hypoxia followed by reoxygenation Pyruvate might thus help to protect of cancer cells during such stressful conditions, which might be harmful for patients with tumors
Results HepG2 cells have greater pyruvate requirements under hypoxic conditions
Pyruvate content was quantified in cell culture medium under normoxic and hypoxic conditions, and in the absence and presence of pyruvate (0.8 mm) in the cul-ture medium (Fig 1) Total oxygen depletion was com-plete after 1 h of incubation in the anaerobic jar (see Experimental procedures) The detection limits of the assay did not allow measurement of intracellular pyru-vate
Under normoxic conditions and in the absence of pyruvate, HepG2 cells produced and secreted pyruvate The extracellular level rose linearly up to 2.2 lmolÆ
mg)1protein after 6 h, corresponding to 0.4 mm in the culture medium When added to the culture medium, the cells consumed pyruvate, with its concentration decreasing slightly and linearly from 0.75 mm to 0.63 mm between 0 and 6 h of incubation
Under hypoxic conditions, and in the absence of exogenous pyruvate, pyruvate excretion by the cells remained identical to that seen under normoxia for the first hour and slowed down during the second hour After 1–2 h of incubation (depending on the experi-ments, data not shown), HepG2 cells stopped secreting pyruvate and consumed it, as shown by the decrease in pyruvate concentration After reoxygenation (data not shown), the production of pyruvate started again until
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
0 1 2 3 4 5 6
Time (hours)
Normoxia with pyruvate Normoxia without pyruvate
Hypoxia with pyruvate Hypoxia without pyruvate
Fig 1 Extracellular pyruvate content in culture medium of HepG2 cells under normoxia (in dark) and hypoxia (in white) when exoge-nous pyruvate (0.8 m M ) is present (circles) or not present (square) The level of extracellular pyruvate was quantified every hour Val-ues are means ± SD of three independent experiments.
Trang 3a level of approximately 0.4 mm was reached When
added to the culture medium, exogenous pyruvate
disappeared linearly at a higher rate than under
norm-oxia up to 3 h, and then more rapidly from 3 to 5 h of
incubation The concentration stabilized at 0.4 mm
The cells seemed to adjust between production and
consumption of pyruvate in order to maintain the
extracellular pyruvate level at about 0.4 mm in the
cul-ture medium This level was maintained after 6 h (data
not shown) This could not be achieved under hypoxia
when no exogenous pyruvate was added Moreover,
these data show that HepG2 cells have a higher uptake
of pyruvate under hypoxic conditions
Pyruvate decreases oxidative stress during
hydrogen peroxide exposure
Antioxidative properties have been widely ascribed to
pyruvate We therefore investigated whether pyruvate
might have those antioxidant capacities in our
experi-mental conditions
HepG2 cells were cultivated in medium enriched
with 5.5 mm glucose in the absence and presence of
pyruvate (0.8 mm) Cells were exposed to increasing
doses of hydrogen peroxide from 25 to 300 lm
Figure 2 shows the level of reactive oxygen species
(ROS) in HepG2 cells with and without pyruvate
The ROS level was lowered in the presence of
pyru-vate for the range of concentrations from 75 to
300 lm hydrogen peroxide At 240 lm exposure and
after normalization to a control without any cells
(100%), the levels of ROS were 71% and 54% for cells
incubated without and with pyruvate, respectively
In our experimental conditions, pyruvate could thus
decrease the generation of oxidative stress induced by
hydrogen peroxide in HepG2 cells and may also have played a direct antioxidant role in HepG2 cells
Exogenous pyruvate protects DNA under hypoxia and after reoxygenation
We wondered whether the increased pyruvate uptake might be related to a protective effect against the con-sequences of hypoxic stress As both hypoxia and reox-ygenation have been reported to induce DNA damage [30–32], we examined the effects of pyruvate addition during hypoxia and after reoxygenation on DNA damage
HepG2 cells were incubated for 6 h under normoxic and hypoxic conditions without and with pyruvate (0.8 mm) DNA fragmentation was estimated with the comet assay The assay was carried out immediately after the 6 h incubation period, and then 1 and 2 h after reoxygenation of the cell cultures (Fig 3)
Under normoxia, DNA fragmentation remained unchanged irrespective of the condition (with or with-out pyruvate) or incubation time
After 6 h under hypoxia and in the absence of pyru-vate, an increase in DNA fragmentation was observed This increase was not observed in the presence of pyruvate One hour after reoxygenation, DNA frag-mentation reached a maximum in both the absence and the presence of pyruvate, and thereafter decreased The increase induced by 1 h of reoxygenation was lower in the presence of 0.8 mm pyruvate, although
10000
12000
0
2000
4000
6000
8000
with pyruvate without pyruvate
H 2 O 2 (µM)
Radical oxygen species (fluorescence units / s)
Fig 2 Pyruvate decreases the level of ROS in the medium of
HepG2 cells exposed to hydrogen peroxide HepG2 cells were
incu-bated with 5.5 m M glucose in the presence of 0.8 m M pyruvate,
and exposed to increasing doses of hydrogen peroxide (from 0 to
300 l M ) Levels of ROS were estimated by spectrofluorometry
using 2¢,7¢-dichlorodihydrofluoresceine bi-acetate The data are from
one representative experiment.
0 5 10 15 20 25 30 35
40
6 h
6 h + 1 h reoxygenation
6 h + 2 h reoxygenation
Normoxia without pyruvate
Normoxia with pyruvate
Hypoxia without pyruvate
Hypoxia with pyruvate
*
*
*
Conditions
DNA fragmentation (Tail extent moment)
Fig 3 Effect of pyruvate on DNA fragmentation induced by hypoxia and reoxygenation in HepG2 cells Cells were incubated for
6 h with glucose (5.5 m M ) in the absence and presence of pyruvate (0.8 m M ) under normoxic or hypoxic conditions Thereafter, cells exposed to hypoxia were incubated under normoxic conditions (reoxygenation) for 1 or 2 h Other conditions and statistical calculations are described under Experimental procedures Values are means ± SD, and P-values ¼ 0.05 are compared in the following way: *hypoxia versus normoxia; with versus without pyruvate
Trang 4nonsignificant After 2 h of reoxygenation, DNA
dam-age returned to normoxic levels in the cells treated
with 1 mm pyruvate, whereas it remained higher in
nontreated cells (47%)
These results show that the presence of pyruvate
significantly reduces DNA fragmentation induced by
hypoxia and reoxygenation Moreover, the comet
assay has recently been described as an efficient
method for detecting DNA repair [33] We therefore
concluded that, after reoxygenation, HepG2 cells
culti-vated with pyruvate repair DNA damage sooner
Exogenous pyruvate restores glutathione levels
after reoxygenation but is ineffective at
maintaining glutathione levels during hypoxia
Glutathione is one of the main antioxidant compounds
in the cell, and it is also essential for DNA synthesis
and repair [34] We wondered whether the beneficial
effect of pyruvate on DNA damage could be mediated
by glutathione To answer this question, we analyzed
the effect of pyruvate on the glutathione content of
HepG2 cells during hypoxia and after reoxygenation
HepG2 cells were incubated for 6 h under normoxic
and hypoxic conditions in the absence and presence of
pyruvate (0.8 mm) Thereafter, hypoxic cells were
re-incubated under normoxic conditions (reoxygenation)
The total intracellular glutathione content (oxidized
and reduced forms) was assayed in the cells
immedi-ately after incubation (6 h), and 1 and 2 h after
reoxy-genation (Fig 4)
Under normoxia, intracellular reduced and oxidized
glutathione levels remained unchanged irrespective of
the presence of pyruvate and incubation time After
6 h under hypoxia, the glutathione content decreased
by approximately 50%, independently of the presence
of pyruvate This profile remained unchanged after 1 h
of reoxygenation After 2 h of reoxygenation, a clear
difference was observed between cells incubated with
and without pyruvate When pyruvate was lacking, the
glutathione levels remained significantly low, whereas
in the presence of the a-keto acid, the glutathione level
was restored
Pyruvate is a precursor of glutathione under
normoxia
To further investigate the effects of pyruvate on
gluta-thione content during hypoxia, we examined pyruvate
metabolism using 13C-NMR The distribution of 13C
labeling was analyzed after incubation of HepG2 cells
in a culture medium containing [13C3]pyruvate, under
normoxia and hypoxia, for 6 h
[13C3]Pyruvate mainly led to the production of
13C-enriched lactate, alanine, glutamate, glutamine and glutathione as previously described (data not shown)
13C-enriched glutamine and glutamate was concomi-tant with the increased abundance of enriched lactate and alanine This is due to the blockade of the tricar-boxylic acid cycle induced by lack of oxygen We examined the peaks corresponding to the glutathione carbons, and quantified the relative abundance of the corresponding molecules under hypoxia and normoxia (Fig 5A) Incubation with [13C3]pyruvate resulted in labeling of [13C2]c-glutamyl glutathione [GSH(c-glut-amyl)], [13C4]c-glutamyl glutathione and [13C3 ]c-glutamyl glutathione Peaks corresponding to [13C3]glutamine and [13C3]GSH(c-glutamyl) were indis-tinguishable, as were those corresponding to [13C2 ]glu-tamine and [13C2]GSH(c-glutamyl) The presence of [13C4]GSH(c-glutamyl) indicated that HepG2 cells con-sumed pyruvate to form glutathione under normoxia Hypoxia induced decreases, respectively, of 92% and 82% in 13C2 and 13C3 glutamine⁄ glutathione peak intensities The [13C4]glutathione peak was reduced by 85% This dramatic decrease indicated that hypoxia induced strong inhibition of the glutathione synthesis pathway from pyruvate
We also analyzed intracellular glutathione (oxidized and reduced form) by enzymatic assay during normoxia
0 20 40 60 80 100 120 140 160
Conditions
normoxia without pyruvate
normoxia with pyruvate
hypoxia without pyruvate
hypoxia with pyruvate
6 h
6 h + 1 h reoxygenation
6 h + 2 h reoxygenation
*
*
*
*
†
Fig 4 Effect of pyruvate on glutathione content during hypoxia ⁄ reoxygenation in HepG2 cells Cells were incubated for 6 h with glucose (5.5 m M ) in the absence and presence of pyruvate (0.8 m M ) under normoxic or hypoxic conditions Thereafter, cells exposed to hypoxia were incubated under normoxic conditions (reoxygenation) for 1 or 2 h Levels of oxidized and reduced gluta-thione were measured in the cell extracts Other conditions and statistical calculations are described under Experimental proce-dures Values are means ± SD, and P-values ¼ 0.05 are compared
in the following way: *hypoxia versus normoxia; with versus without pyruvate
Trang 5and hypoxia (from 1 to 6 h, Fig 5B) In both the
pres-ence and the abspres-ence of pyruvate, a regular
time-depen-dent increase in glutathione content occurred under
normoxia, whereas no variation was observed under
hypoxia After 3 h of incubation, the glutathione level in
cells exposed to hypoxia was significantly lower than in
cells exposed to normoxia, confirming that addition
of exogenous pyruvate failed to restore glutathione
synthesis
To confirm that the unchanged intracellular
glutathi-one was due to inhibition of synthesis and not simply
excretion from the cell during hypoxia, we also analyzed
extracellular levels after 6 h of incubation (Fig 5C
shows intracellular and extracellular glutathione levels
and the sum of both) Even though the extracellular
content increased in response to hypoxia, indicating
release by the cells, the sum of both contents showed an
overall decrease in the glutathione level This effect was independent of pyruvate supplementation
Together, these data indicate that pyruvate is a precursor of glutathione under normoxic conditions through glutamate generated by oxidative metabolism (Fig 6) However, pyruvate cannot be used as a gluta-thione precursor under hypoxia, because of the lack of oxygen
Discussion Modulation of the fate of pyruvate fate in one direction
or another can be important for adaptive responses to hypoxia followed by reoxygenation [26,35] Repression
of pyruvate dehydrogenase (EC 1.2.4.1) and switching between the highly active tetrameric and the inactive dimeric forms of pyruvate kinase (EC 2.7.1.40) are observed in tumors [2,3] Such alterations of pyruvate metabolism could be advantageous for cancer cells under such stressful conditions [36] Our present work provides new insights into the role of pyruvate in tumor cells during hypoxia We show here that tumor HepG2 cells are inclined to maintain extracellular pyruvate at a constant level (0.4 mm) Hypoxia inhibits such regula-tion, whereas pyruvate supplementation restores it We also observed that hypoxic cells increase their consump-tion of exogenous pyruvate and stop releasing it when the a-keto acid is absent from the medium Our data indicate that the endogenous need for pyruvate increases under hypoxia Pyruvate protects cells from DNA breaks induced by both hypoxia and reoxygenation
0.0
[4- 13 C]-GSH [3- 13 C]- GSH
and/or -Gln 0.5
1.0
1.5
2.0
2.5
3.0
3.5
2.52
0.20
normoxia hypoxia
[2- 13 C]-GSH
and/or - Gln
13 C-enriched metabolites
175
0
25
50
75
100
125
150
0 1 2 3 4 5 6
Time (hours)
*
*
Normoxia without pyruvate
Normoxia with pyruvate
Hypoxia without pyruvate
Hypoxia with pyruvate
0
25
50
75
100
125
150
175
intracellular
Normoxia
with pyruvate
Hypoxia
without pyruvate
Normoxia with
pyruvate
Hypoxia with
pyruvate
Localization
A
B
C
Fig 5 Effect of hypoxia and pyruvate on glutathione synthesis by HepG2 cells (A) Glutathione13C labeling following incubation of the cells with 13 C-enriched pyruvate: cells were incubated for 6 h with [ 13 C3]pyruvate and 5.5 m M glucose under normoxic and hypoxic conditions After NMR analysis, the peaks corresponding to [13C 3 ]glu-tamine (Gln) and ⁄ or [ 13 C3]GSH (c-glutamyl), [ 13 C4]GSH(c-glutamyl), and [ 13 C2]glutamine and ⁄ or [ 13 C2]GSH(c-glutamyl) were identified The relative amount was quantified by integration of the peak area (B) Intracellular kinetics of intracellular glutathione (oxidized and reduced) concentration: biochemical measurement of total glutathi-one was performed every hour HepG2 cells were cultivated in the absence (squares) and presence (circles) of pyruvate under normoxic (in black) or hypoxic (in white) conditions Other conditions and statistical calculations are described under Experimental procedures Values are means ± SD; *P ¼ 0.05 as compared with corresponding normoxic condition (C) Distribution of total glutathione in the intra-cellular and extraintra-cellular compartments: HepG2 cells were incubated for 6 h under normoxic and hypoxic conditions in the absence and presence of pyruvate (1 m M ) Intracellular and extracellular total glu-tathione contents were assayed separately The sum of both is also shown Values are means ± SD; *P ¼ 0.05 as compared with the corresponding normoxic condition.
Trang 6That is not well correlated with the beneficial effect on
the antioxidant glutathione Actually, pyruvate restores
glutathione levels only during reoxygenation, and has
no effect under hypoxia We demonstrate here that the
a-keto acid is a precursor of glutathione through the
tri-carboxylic acid cycle, and that hypoxia severely impairs
its biosynthesis
Regulation of extracellular pyruvate content has
pre-viously been reported in tumor cells as well as in
non-tumor cells [37] This mechanism is assumed to be a
way for cells to lower oxidative stress Pyruvate has
antioxidant properties [21,22] that could possibly also
be manifested under our experimental conditions The
phenomenon described by O’Donnell-Tormey might
well take place in HepG2 cells
Impairment of the regulation of the extracellular
content and the increase of its uptake induced by
hypoxia show that alteration of pyruvate metabolism
takes place The greater uptake might be due to an
increased need to maintain antioxidant capacity in the
cells However, it might also result from a metabolic
requirement Pyruvate increases the ratio of lactate
production to glucose consumption (+ 19%, data not
shown) This is in line with our previous work [29]
indicating enhancement of glycolysis activity in the presence of pyruvate Increased glycolysis might thus maintain the ATP supply during oxygen deprivation while oxidative phosphorylation is seriously impaired Such an increase has been reported to have protective effects against hypoxic stress [38,39]
Hypoxia, known to alter trhe antioxidant system, affects glutathione metabolism as well In our experi-mental conditions, we observed a decrease of intracel-lular content together with release of the glutathione
In rat primary hepatocytes in vitro, hypoxia induced activation of the glutathione transporters, resulting in increased glutathione export [40] A similar mechanism might occur in HepG2 cells, where glutathione trans-port is functional [41] Furthermore, our results also show that hypoxia induces a decrease in total glutathi-one Pyruvate has no effect on the hypoxia-induced decrease of glutathione content, whereas it allows complete restoration of total glutathione after reoxy-genation Hypoxia-induced inhibition of glutathione synthesis from pyruvate might be the main mechanism responsible for such an effect Under normoxia, we demonstrate that pyruvate supplies glutamate through oxidative metabolism in tumor cells Inhibition of the
Glutamate
Mitochondrion
2 × FAD +
Glucose
2 × Pyruvate
2× Lactate
Alanine
2 × NADH, H+
2 × NAD +
Glycolysis
2NAD + + 2ADP + Pi
2NADH, H + + 2ATP
Pyruvate
2 × NADH, H + + 2 × CO2 2×NAD +
Krebs Cycle
2×
2×Acetyl-CoA CoA
CoA
4×CO 2
6 ×NADH, H +
6 × NAD +
2ADP + Pi 2ATP
2 × FADH, H +
Cytosol
Glutamate
5-Oxoproline
γ-Glutamyl-cysteine Cysteine
Glycine
ADP + Pi
+ ATP
+ ATP ADP + Pi
Glutamine
GS-X (conjugate) Amino acids (AA)
γ-Glutamyl
-AA
Cysteinyl-glycine
γ-Glutamyl
-AA
Cysteinylglycine
conjugate
Extracellular area
2×
Glutathione GSH
Fig 6 Pathway of glutathione synthesis from pyruvate, showing how pyruvate, through the tricarboxylic acid cycle, is involved in glutamate synthesis and then in the formation of glutathione (GSH).
Trang 7tricarboxylic acid cycle thus results in significant
inhi-bition of glutathione synthesis Usually, cysteine is
assumed to be the limiting precursor of the two-step
reaction leading to glutathione synthesis However,
glutamate plays an important role in glutathione
syn-thesis [3] Negative feedback from glutathione itself on
the step catalyzed by glutamate-cysteine ligase can be
prevented by glutamate [1,2] It might even become
limiting under conditions of mitochondrion blockage
in muscle [4] and during hypoxia in glial cells [5]
Pyru-vate enhances glycolysis activity during hypoxia in
HepG2 cells [29] By providing more substrates for the
tricarboxylic acid cycle and through indirect effects on
redox status, pyruvate might thus favor restoration of
the glutamate pool and then the glutathione pool after
reoxygenation (Fig 6)
Induction of DNA damage by hypoxia and
reoxygen-ation is a well-known phenomenon mainly caused by
ROS [30–32] A functioning antioxidant system is
essen-tial to reduce such damage Despite the absence of an
effect on glutathione, pyruvate has beneficial effects on
DNA breaks under hypoxia Many studies have
reported that pyruvate improves antioxidant capacities
and protects normal tissues against damage induced
by hypoxia⁄ reoxygenation and ischemia ⁄ reperfusion
[23,26] The increased uptake of pyruvate might allow
HepG2 cells to maintain their antioxidative capacities
in a way independent of glutathione during hypoxia
However, after reoxygenation, the beneficial effect of
pyruvate against DNA breaks is well correlated with
glutathione restoration DNA breaks decrease faster in
cells treated with pyruvate, suggesting stimulation of
DNA repair As glutathione is essential for DNA
syn-thesis in general, as well as for DNA repair [34], the
action of pyruvate on glutathione might also favor
DNA repair During hemorrhagic shock and ischemia
followed by reperfusion, pyruvate interferes with
poly-(ADP-ribose)-polymerase (EC 2.4.2.30) activity by
pre-venting loss of total NAD+ content [19,20,42] This
might enhance DNA repair Pyruvate thus plays a role
in DNA protection and repair in tumor HepG2 cells
during hypoxia and after reoxygenation The
mecha-nism might involve both antioxidant properties and
metabolic activity The exact mechanism underlying the
pyruvate effects has yet to be further examined
In light of the data in the literature and our work
with HepG2 cells, we concluded that pyruvate might
act similarly in tumor cells and nontumor cells It could
then act as a protective agent against DNA damage
under conditions of tumor hypoxia Many strategies
for cancer therapy are based on increases in the number
of DNA strand breaks (e.g radiotherapy and
alkylat-ing agents) Pyruvate might thus reduce the efficiency
of treatment by limiting DNA damage and favoring repair Pyruvate also stimulates angiogenesis [4,6]; this might favor tumor reoxygenation and progression Fur-thermore, a high intracellular glutathione level corre-lates with a high level of proliferation of tumor cells [43] and with resistance to anticancer treatment [44,45] Pyruvate might support a high glutathione level in reoxygenated tumors All of these effects might favor tumor development and lower efficacy of some thera-pies They remain to be verified in vivo in tumors If they were verified, it would confirm that no matter how pyruvate acted, it would be deleterious in cancer
In conclusion, this study confirms the importance and the multilevel and very complex implications of pyruvate for cell responses to hypoxia⁄ reoxygenation Our results are in agreement with current literature identifying pyruvate as a protector of normal and tumor cells subjected to hypoxia Furthermore, this study provides additional data confirming that what-ever the underlying mechanisms at work, the presence
of pyruvate is undesirable in tumor cells Indeed, con-trolling pyruvate levels might be an advantageous way
of modulating tumor resistance and improving the efficiency of certain cancer therapies
Experimental procedures Cell culture
A human hepatocellular carcinoma HepG2 cell line was purchased from the American Type Cell Collection It derives originally from a hepatocellular carcinoma biopsy and synthesizes nearly all human plasma proteins [46] The cell line is not tumorigenic in immunosuppressed mice Cells, used between passages 76 and 82, were grown in Petri dishes coated with type 1 collagen (10 lgÆmL)1,
60 lLÆcm)2, 30 min at 37C; Sigma-Aldrich, Saint-Quentin Fallavier, France) in DMEM (Sigma-Aldrich) supple-mented with 10% fetal bovine serum (Biowhittaker, Cambrex Corporate, East Rutherford, NJ), antibiotics (100 000 UÆL)1 penicillin, 100 mgÆL)1 streptomycin; Boeh-ringer Ingelheim, Paris, France), 4 mm glutamine (Jacques Boy Institute, Reims, France), 1% nonessential amino acids and 1 mm pyruvate (Seromed Biochrom KG, Berlin, Ger-many) in a 95% air⁄ 5% CO2humidified atmosphere Half
of the medium was changed every 2 days The cell cultures were split at confluence with 0.25% (w⁄ v) trypsin (Jacques Boy Institute) and seeded at a density of 4· 104cellsÆcm)2 For the experiments, cells were used at subconfluency (2 days after passage) as determined by the growth curve based on cell numbers and protein quantification
To rule out the presence of mycoplasma contamination, tests were performed using a commercially available detection kit (Polylabo⁄ VWR International,
Trang 8Fontenay-sous-Bois, France) Cell viability was routinely determined
using Trypan Blue exclusion
Incubation of cells
Cells were incubated as previously described [29] Briefly,
subconfluent HepG2 cells were incubated for 6 h with
DMEM base (Sigma-Aldrich) containing 5.5 mm glucose,
10 mm Hepes (Sigma-Aldrich), 10% fetal bovine serum,
antibiotics (100 000 UÆL)1 penicillin, 100 mgÆL)1
strepto-mycin), 1% nonessential amino acids and 4 mm glutamine,
both without and with pyruvate (1% of a 100 mm stock
solution, 0.8 mm final), in a normoxic atmosphere with
5% CO2 and under low oxygen pressure in anaerobic jars
The oxygen content in the jars was monitored with an
oxy-gen electrode (O2sensor; Mettler-Toledo, Viroflay, France)
Similar results were obtained by incubating the cells in an
oxygen-depleted atmosphere using a cell culture incubator
with N2, O2 and CO2 control (Queue Systems Inc.,
Ashe-ville, NC) For the reoxygenation experiments, cells
incu-bated under hypoxia were removed from the jar and placed
in a normoxic atmosphere with 5% CO2
Extraction and quantification of extracellular
pyruvate
Perchloric acid (PCA) (8%, v⁄ v) was added to the culture
medium (2 : 1, v⁄ v) After homogenization, the mixture
was centrifuged
swing-out rotor; Jouan, Saint-Herblain, France) and the
supernatant was stored at) 80 C prior to assay
Pyruvate was quantified by the method previously
described by Marbach & Weil [47], with slight
modifica-tions Briefly, the assay was carried out in a 96-well plate
with 160 lL of sample (PCA extract), 40 lL of Tris⁄ HCl
buffer (1.5 m Trizma base with 0.05% w⁄ v
pH 10.5; Sigma-Aldrich) and 40 lL of a mixture (0.3 : 2,
v⁄ v) of NADH-Na2(4.55 mgÆmL)1) and Trizma base buffer
per well First, the absorbance was measured at 340 nm:
10 lL of lactate dehydrogenase (EC 1.1.1.27) (400 UÆmL)1
in 3.2 m ammonium sulfate, pH 6.5) was added to each
well, and the absorbance was measured after complete
sta-bilization The absorbance at 340 nm resulting from the
oxidation of NADH to NAD reflected the amount of
pyru-vate originally present in the sample The pyrupyru-vate sample
concentration was determined according to a standard
curve established between 0 and 0.5 mm pyruvate
Comet assay
The comet assay was performed according to the method
described by Singh et al [48], using alkaline electrophoresis,
which allows detection of single-strand and double-strand
breaks
Cells cultivated in Petri dishes (25 mm in diameter) were suspended in 0.5 mL of NaCl⁄ Pi, and cell density was estimated with a Malassez slide An aliquot of the suspension was added to low molecular weight agarose (0.8%, p⁄ v in NaCl ⁄ Pi) to obtain a final concentration of
25· 104
cellsÆmL)1 Eighty microliters of agarose-sus-pended cells was placed on an agarose-coated slide (high molecular weight, 1% p⁄ v in NaCl ⁄ Pi) and covered with
a coverslip After 5 min on ice, the coverslip was gently removed The slides were incubated (1 h, 4C in the dark) in lysis buffer (45 mL of buffer containing 2.5 m NaCl, 0.1 m EDTA, 10 mm Tris with 5 mL of dimethyl-sulfoxide and 0.5 mL of Triton X-100, pH 10) The slides were rinsed twice with electrophoresis buffer (300 mm NaOH, 1 mm EDTA) for 10 min, and then for 25 min Electrophoresis was carried out at 25 V and 300 mA for
37 min at room temperature Finally, the slides were washed with Tris⁄ HCl buffer (pH 7.5) DNA was stained using an ethidium bromide solution (0.1 mgÆmL)1, 50 lL per slide, kex¼ 525 nm and kem¼ 650 nm) The slides were read with an epifluorescence microscope
with a CDD camera (Zeiss Axioskop20, Carl Zeiss, Microscope Division, Oberkochen, Germany)
were analyzed with the Komet 3.0 image analysis system (formerly by Kinetic Imaging; now Andor Technology, Belfast, UK) Fragmentation was expressed in Tail Extent Moment, taking into account tail length and the percent-age of DNA in the comet tail Impercent-ages of 500 randomly selected cells were analyzed from each sample
Extraction and quantification of glutathione After incubation, 0.5 mL of water was added to the Petri dishes (100 mm in diameter) At a low temperature, the cell monolayer was removed by scraping Cells were collected in
a 5 mL tube, and the volume was adjusted to 1 mL before addition of 0.2 mL of metaphosphoric acid (6%, p⁄ v) After shaking (30 s), the mixture was centrifuged
10 min, 4C, CR3i centrifuge and swing-out rotor) The pellet was used for protein quantification by the Folin– Lowry method [49] The supernatant was kept at ) 80 C prior to glutathione assay
In the case of medium samples, 0.2 mL of metaphos-phoric acid (6%, p⁄ v) was added to 1 mL of culture med-ium and treated as described above
Total glutathione (oxidized and reduced forms) was quantified as previously described by Tietze [50] The assay was performed in a 96-well plate Twenty microliters
of sample was placed in each well with 150 lL of Mops⁄ EDTA buffer containing 0.165 UIÆmL)1 glutathione reduc-tase Then, 0.267 mgÆmL)1 b-NADPH and 75 lL of 5,5¢-dithiobis(2-nitrobenzoic acid) solution (0.04 mg mL)1
in 0.4 m Mops⁄ 2 mm EDTA, pH 6.75) were added succes-sively After shaking, the absorbance was measured at
Trang 9412 nm using a plate reader spectrophotometer (Thermo
Clinical Labsystems France, Cergy Pontoise, France)
A standard curve was obtained with a commercial
gluta-thione solution (20 mm, Sigma-Aldrich) Standard samples
were treated for extraction using the protocol described for
biological samples
PCA extraction and13C-NMR analysis of cell
extracts
For 13C-NMR, the cell line was incubated in the presence
of 5.5 mm glucose (unenriched) with 1 mm [13C3]pyruvate
(Euriso-Top, Saint Aubin, France) Cells were exposed to
both normoxic and hypoxic conditions
After 6 h of incubation, the medium was discarded and
the cultures were washed twice with cold NaCl⁄ Pi,
immedi-ately frozen in liquid nitrogen, and stored at ) 80 C until
further treatment PCA extraction was performed following
standard procedures Briefly, 0.3 mL of 12% PCA was
added to the Petri dishes (100 mm in diameter), the cell
monolayer was removed by scraping with a spatula, and the
cell suspensions obtained from seven individual Petri dishes
were pooled After homogenization, the final cell suspension
was centrifuged
swing-out rotor), and the supernatant was adjusted to
pH 7.4 with KOH The samples were again centrifuged
8000 g for 10 min (CR3i centrifuge and swing-out rotor),
the supernatant was lyophilized, and the dry residue was
dissolved in 2.2 mL of H2O⁄ 20% D2O for NMR analysis
Proton-decoupled spectra of PCA extracts were recorded
on a Bruker AM400
with a 10 mm 31P⁄13
C probe (Bruker, Wissembourg, France) 13C-NMR spectra were recorded at 100.62 MHz,
and each spectrum was the sum of 15 000 free induction
decays A 90 pulse was applied, with a repetition time of
3 s and an acquisition time of 0.819 s The temperature was
maintained at 20C, and the13
C chemical shifts were refer-enced to the resonance of tetramethylsilane at 0 p.p.m
Peak intensities were normalized to the peak intensity of
ethylene glycol, used as an internal reference Relative areas
of the peaks were normalized to the area of the reference
peak, arbitrarily fixed at 100 The NMR analysis of the
PCA extracts was reproducibly repeated four times for both
normoxic and hypoxic conditions The data shown are
from a representative experiment
ROS measurement
The determination of intracellular oxidant production is
based on the oxidation of 2¢,7¢-dichlorodihydrofluorescein
(Sigma-Aldrich) to the fluorescent 2¢,7¢-dichlorofluorescein
HepG2 cells were incubated in the absence and presence of
pyruvate (1 mm) in DMEM base containing 5 mm glucose
in the fluorescence spectrometer After addition of
2¢,7¢-dichlorodihydrofluorescein (10 lgÆmL)1), fluorescence
emission was measured continuously at 520 nm after excita-tion at 499 nm The value of the slope was proporexcita-tional to the intracellular ROS levels
Statistical analysis of results The experiments were reproducibly repeated four times Values are means ± standard deviation (n¼ 6 separate Petri dishes) Statistical analysis of the data was done by anova The Newman–Keuls unpaired t-test was used to determine statistical significance
Acknowledgements The present study was partially funded by the Institut Fe´de´ratif de Recherche (IFR-1) ‘Biomedical NMR: From Cell to Man’ (Grenoble) In particular, we owe thanks to Professor J.-F Le Bas
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