Pectinase production by aspergillus niger using banana (musa balbisiana) peel as substrate and its effect on clarification of banana juice

Giảng viên: Nguyễn Đức ChungBộ môn: Công nghệ enzyme Lớp: CNTP 52C Khoa: Cơ khí công nghệ Pectinase production by Aspergillus niger using banana Musa balbisiana peel as substrate and it

Giảng viên: Nguyễn Đức Chung

Bộ môn: Công nghệ enzyme Lớp: CNTP 52C

Khoa: Cơ khí công nghệ

Pectinase production by Aspergillus niger using banana (Musa

balbisiana) peel as substrate and its effect on clarification of

banana juice 

I INTRODUCTION

Pectinase is a general term for enzymes, such as pectin lyase, pectin methylesterase and polygalacturonase, commonly referred to as pectic enzymes These break down pectin, a polysaccharide substrate that is found in the cell walls of plants

The pectinase enzyme is obtained mainly by fungi, in which Aspergillus niger is believed to be capable of producing many pectinase enzymes when cultured under suitable media

I INTRODUCTION

Polygalactorunase obtained from Aspergillus niger cultured on banana peel substrate has the effect of making banana juice

To optimize crude pectinase production using Bhimkol banana peel as

substrate for higher polygalacturonase activity

II Materials

Banana peel is an abundant and low cost agricultural waste residue It accounts for about 40 % of the weight of the raw fruit and is rich in carbohydrates,

protein and various vitamins and mineral elements They have been used for the production of pectinases

 Substrate preparation for enzyme production:

- The bananas were washed under tab water and peeled with hand The peels were then dried at

45 °C for 24 h and then grinded using commercial grinder and passed through 200 mesh sieve to obtain banana peel powder The powder thus produced was then used as substrate for the production of Pectinase enzyme.

III Pectinase production:

Pectinase production:

 Experiments were carried out in 250 ml Erlenmeyer flasks containing KH2PO4 (0.02 % w/v), MgSO4 (0.01 % w/v),

(NH4)2 SO4 (0.04 % w/v), FeSO4 (0.01 % w/v), MnSO4 (0.001 % w/v)

in 100 ml distilled water

 The medium was sterilized and cooled to room temperature

 The pH of the medium: 5,8 (using citrate buffer)

III Pectinase production:

The sterilized flasks were inoculated 

incubation in an incubator shaker

pectinase centrifuge

incubation the culture filtrate was cooled to 4 °C

 2 × 10 8 spores of A

niger

speed of 150 rpm

10,000 rpm for

10 min

 in

refrigerated

centrifuge.

Partial crude enzyme:

-

III Pectinase production:

GIỚI THIỆU