Các mũi nhọn Công nghệ sinh học hiện nay và các tiếp cận tại Việt Nam... Công nghệ tê bào mâm Stem cell producing cells... Công nghệ tê bào mam Stem cell... dNTP, DNA polymerase, ddATP
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Các mũi nhọn Công nghệ
sinh học hiện nay và các
tiếp cận tại Việt Nam
Trang 2¬
Net
sen
m Cena
n Tri Phuong va BV An Binh
inh — Khoa Y - DHYD
Trang 3© Plasmid put into
Trang 4Đề có gen mong muôn
Trang 5KY thuat Southern blotting dé do duoc
doan gen mong muon
Trang 6Dung PCR dé nhan ban gen
PCR
Té bao
Trang 7Phai di tu
mRNA néu
la gen co
Intron
Trang 9Công nghệ nhân bản động vat
The making of a clone
u unfertilised nee 2 with Y ‘s mitochondria
Sheep Y 2) =- Y ‘s mitochondria in e
Trang 10Recipient Blastocyst
Trang 12Công nghệ tê bào mâm (Stem cell)
producing cells
Trang 13Công nghệ tê bào mam (Stem cell)
Trang 16Dopamine-Neuron Transplantation
Stem cell tri
Parkinson
Before Surgery After Surgery
Figure 8.3 Positron Emission Tomography (PET) images from a Parkinson's patient
before and after fetal tissue transplantation The image taken before surgery (left)
shows uptake of a radioactive form of dopamine (red) only in the caudate nucleus,
indicating that dopamine neurons have degenerated Twelve months after surgery, an
image from the same patient (right) reveals increased dopamine function, especially
in the putamen (Reprinted with permission from N Eng J Med 2001;344 (10) p 710.)
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4 injected into the muscle of c®? stem cells are injected into (4 3
Trang 18+ delivery vehicle \ 4 a’ delivery vehicle `
| delivery cell such as
/ a stem cell that Is
i \ often derived from
| The genetically modified
| cells (e.g., stem cells)
are multiplied in the
Trang 20Cong nghe DNA
therapy
Cloned gene
(normal
from | - @ insert normal allele
patient's | | into retrovirus
7 © Let retrovirus infect bone
patient and cultured
€) Viral DONA carrying the
normal allete inserts into chromosome
2
marrow cell from
cells into patient
C12PỚAA Abbe Weary (uy ée re
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dNTP, DNA polymerase, ddATP
dNTP, DNA polymerase, ddCTP
dNTP, DNA polymerase, ddGTP
dNTP, DNA polymerase, ddT TP
CGTAAGGdC CGTAAGGCCACGdT CGTAAGGCdC CGTAAGGCCACGTAdT CGTAAGGCCAdC | |
CGTAAGGCCACGTATdG a
CGTAAGGCCACGTdA a CGTAAGGCCACGdT " oo
CGTAAGGCCACdG "¬ Chiêu điện di
CGTAAGGCCAdC a CGTAAGGCCdA a CGTAAGGCdC "
Trang 24Cong ngheé microarray
Trang 26Công nghệ chân đoán nhanh bằng sắc ký miễn dịch
Vd: Phat hiện S pyogenes trong quyet hong
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Công nghệ kháng thê đơn dòng
Lấy lách chuột ———> XS
|
Nghiền qua luới
để tách các tế bào sản xuất kháng
thể
Gây miền dịch trên chuột
⁄⁄/Tếbào sản xuất ` Hoà nhập TB sx KT
kháng thể bị với TB K tủy chuột
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Sản xuất KTĐD bằng công nghệ gen
Gene transfer Immunization
Trang 30Sản xuất KTĐD bằng công nghệ gen
Đoạn gen chu trách
Trang 32Nanoengineered Smart Particles
Công nghệ sinh học nano
Trang 35Công nghệ PCE phát hiện bệnh
ADN đích
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»% www.nk-biotek.com.vn
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Cudi 11/2004, tổ
chức chứng nhận
RWTUV Asia
Pacific la thanh viên
tap doan TUV Nord
cong nhan dat tiéu
procedure that NAM KHOA MOLECULAR BIOLOGY COMPANY
793/58 Tran Xuan Soan Street, District 7,
Ho Chi Minh City, Vietnam
has established and applies a quality system for
Manufacturing and Supply of Bio — laboratorial Material and Equipment: Bio — cultures media, Toolkit Test for Disease Detection, Bio - chemical for
Health Services and Biotechnologies
An audit was performed, Report No 2.5-4503/2005 Proof has been furnished that the requirements according to
Trang 38QUALITY SYSTEM ASSESSMENT
TEMPORARY STATEMENT to Certificate No 14-NQA/TIBEAM/2005
This ts to certify that the Quality System of
NAM KHOA BIOTECHNOLOGY CO., LTD
7958 Tran Kuan Soan - Tan Hung Ward - District 7 - TPHCM
has Deen assessed and found to conform to, subject to any outstanding matter and subject to completed review of Assessment Report by the Accreditation Management Representative the Quality System Standards :
ISO 9001:2000, WHO GMP/GLP
The final certificate will be valid for the following product or service ranges
Manufacture and Supply of
* Blo-aboratorial Materials and Equipments
+ Blo<ultures media + Tool kit test for clsease detection
* Blo-Chemical for Health Services and Biotechnologies
Accredited by the URAS, London
Trang 40Bracnnd nanan
Trang 41Công nghệ lây dâu vân tay DNA
(DNA finger printing)
Trang 43ig
Botte
„2 5g
